(Circular) (Six-base) MAPSORT of: pFN21AE1645.seq Check: 8555 from: 1 to: 5185 pFN21AE1645 5185 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2995 278 Size: 53 83 186 936 1459 2468 Fragments arranged by size: 2468 1459 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2528 1937 Size: 591 4594 Acc65I G'GTAC_C Cuts at: 2506 2506 Size: 5185 AclI AA'CG_TT Cuts at: 1563 3312 3685 1563 Size: 1749 373 3063 Fragments arranged by size: 3063 1749 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2073 2348 3248 4632 4900 1632 Size: 441 275 900 1384 268 1917 Fragments arranged by size: 1917 1384 900 441 275 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4988 AflIII A'CryG_T Cuts at: 1203 4084 5061 1203 Size: 2881 977 1327 Fragments arranged by size: 2881 1327 977 AgeI A'CCGG_T Cuts at: 4016 4016 Size: 5185 AhdI GACnn_n'nnGTC Cuts at: 3914 3914 Size: 5185 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5185 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5185 AlwNI CAG_nnn'CTG Cuts at: 2928 4500 2928 Size: 1572 3613 ApaLI G'TGCA_C Cuts at: 3242 4398 3242 Size: 1156 4029 ApoI r'AATT_y Cuts at: 1493 2378 2494 2720 2872 4981 1493 Size: 885 116 226 152 2109 1697 Fragments arranged by size: 2109 1697 885 226 152 116 AseI AT'TA_AT Cuts at: 160 1032 2142 3739 160 Size: 872 1110 1597 1606 Fragments arranged by size: 1606 1597 1110 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5185 AvaI C'yCGr_G Cuts at: 1870 1943 2510 1870 Size: 73 567 4545 Fragments arranged by size: 4545 567 73 BamHI G'GATC_C Cuts at: 2171 2515 2882 2171 Size: 344 367 4474 Fragments arranged by size: 4474 367 344 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2506 3961 618 Size: 325 217 627 719 1455 1842 Fragments arranged by size: 1842 1455 719 627 325 217 BanII G_rGCy'C Cuts at: 1391 2326 2429 2504 1391 Size: 935 103 75 4072 Fragments arranged by size: 4072 935 103 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4214 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3376 3410 1903 Size: 34 1439 34 3678 Fragments arranged by size: 3678 1439 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3077 4287 3077 Size: 1210 3975 BclI T'GATC_A Cuts at: 1548 1548 Size: 5185 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3796 136 Size: 107 122 71 631 605 2124 1525 Fragments arranged by size: 2124 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5180 5180 Size: 5185 BlpI GC'TnA_GC Cuts at: 2602 2602 Size: 5185 Bme1580I G_kGCm'C Cuts at: 1790 3246 4402 1790 Size: 1456 1156 2573 Fragments arranged by size: 2573 1456 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5185 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2916 3874 442 Size: 416 53 83 399 24 1499 958 1753 Fragments arranged by size: 1753 1499 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5185 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3845 1239 Size: 2606 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3310 4175 4473 3310 Size: 865 298 4022 Fragments arranged by size: 4022 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3848 882 Size: 617 2349 2219 Fragments arranged by size: 2349 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2976 493 Size: 711 1772 2702 Fragments arranged by size: 2702 1772 711 BsaBI GATnn'nnATC Cuts at: 5179 5179 Size: 5185 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2992 3374 4009 275 Size: 53 83 186 936 1459 382 635 1451 Fragments arranged by size: 1459 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3618 4016 4290 4437 4848 3618 Size: 398 274 147 411 3955 Fragments arranged by size: 3955 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2325 2355 1178 Size: 30 165 30 922 30 4008 Fragments arranged by size: 4008 922 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5185 BseYI C'CCAG_C Cuts at: 4388 4884 4388 Size: 496 4689 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4612 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2635 3396 4424 1540 Size: 275 186 634 761 1028 2301 Fragments arranged by size: 2301 1028 761 634 275 186 BsiHKAI G_wGCw'C Cuts at: 2326 3246 3331 4402 2326 Size: 920 85 1071 3109 Fragments arranged by size: 3109 1071 920 85 BsmI GAATG_Cn' Cuts at: 2696 2789 2696 Size: 93 5092 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2326 2429 2504 3246 3331 4402 1391 Size: 399 536 103 75 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 536 399 103 85 75 BspEI T'CCGG_A Cuts at: 4848 4848 Size: 5185 BspHI T'CATG_A Cuts at: 3074 4804 3074 Size: 1730 3455 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2540 844 Size: 1696 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3072 1394 Size: 429 1249 3507 Fragments arranged by size: 3507 1249 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3680 3854 65 Size: 1183 2432 174 1396 Fragments arranged by size: 2432 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3829 4016 1700 Size: 110 2019 187 2869 Fragments arranged by size: 2869 2019 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5185 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5185 BssSI C'ACGA_G Cuts at: 3245 4257 3245 Size: 1012 4173 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5185 BstBI TT'CG_AA Cuts at: 3989 3989 Size: 5185 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5185 BstYI r'GATC_y Cuts at: 1972 2171 2294 2515 2882 3267 3284 4736 4822 Size: 199 123 221 367 385 17 1452 86 Cuts at: 4822 4834 5180 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 221 199 123 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4633 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4446 Fragments arranged by size: 4446 683 56 BtsI GCAGTG_nn' Cuts at: 2701 3495 3515 2701 Size: 794 20 4371 Fragments arranged by size: 4371 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5185 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5150 DraI TTT'AAA Cuts at: 2217 2489 2217 Size: 272 4913 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4192 809 Size: 507 2876 1802 Fragments arranged by size: 2876 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2632 3521 5150 Size: 54 1425 50 275 18 802 889 1629 Cuts at: 5150 8 Size: 43 Fragments arranged by size: 1629 1425 889 802 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2632 1537 Size: 275 820 4090 Fragments arranged by size: 4090 820 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3115 1353 Size: 1762 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3772 4146 4292 387 Size: 1064 138 2183 374 146 1280 Fragments arranged by size: 2183 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2324 2324 Size: 5185 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2073 2348 3248 3845 4632 4900 1239 Size: 393 441 275 900 597 787 268 1524 Fragments arranged by size: 1524 900 787 597 441 393 275 268 EcoRI G'AATT_C Cuts at: 2494 2872 2494 Size: 378 4807 FspI TGC'GCA Cuts at: 3691 3691 Size: 5185 HaeII r_GCGC'y Cuts at: 1678 4332 1678 Size: 2654 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2294 2326 3826 3858 3900 Size: 32 135 32 722 32 1500 32 42 Cuts at: 3900 3932 1373 Size: 32 2626 Fragments arranged by size: 2626 1500 722 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2149 2251 2529 2775 669 Size: 988 281 211 102 278 246 3079 Fragments arranged by size: 3079 988 281 278 246 211 102 HindIII A'AGCT_T Cuts at: 748 748 Size: 5185 HpaI GTT'AAC Cuts at: 2149 2775 2149 Size: 626 4559 KpnI G_GTAC'C Cuts at: 2510 2510 Size: 5185 MfeI C'AATT_G Cuts at: 2784 2784 Size: 5185 MluI A'CGCG_T Cuts at: 5061 5061 Size: 5185 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4299 4483 1305 Size: 2994 184 2007 Fragments arranged by size: 2994 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3363 Fragments arranged by size: 3363 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3143 3502 3661 518 Size: 648 1977 359 159 2042 Fragments arranged by size: 2042 1977 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2601 3278 4426 4671 1606 Size: 995 677 1148 245 2120 Fragments arranged by size: 2120 1148 995 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4633 NdeI CA'TA_TG Cuts at: 387 387 Size: 5185 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5185 NotI GC'GGCC_GC Cuts at: 2632 2632 Size: 5185 NspI r_CATG'y Cuts at: 1132 1229 2543 4088 1132 Size: 97 1314 1545 2229 Fragments arranged by size: 2229 1545 1314 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5185 PciI A'CATG_T Cuts at: 4084 4084 Size: 5185 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5185 PmeI GTTT'AAAC Cuts at: 2489 2489 Size: 5185 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3254 3286 4767 4799 3254 Size: 32 1481 32 3640 Fragments arranged by size: 3640 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5185 PsiI TTA'TAA Cuts at: 2755 2755 Size: 5185 PstI C_TGCA'G Cuts at: 830 2537 830 Size: 1707 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5185 SacI G_AGCT'C Cuts at: 2326 2326 Size: 5185 SalI G'TCGA_C Cuts at: 1936 2527 1936 Size: 591 4594 SbfI CC_TGCA'GG Cuts at: 2537 2537 Size: 5185 ScaI AGT'ACT Cuts at: 3433 5145 3433 Size: 1712 3473 SfcI C'TryA_G Cuts at: 826 1045 2533 3668 4349 4540 826 Size: 219 1488 1135 681 191 1471 Fragments arranged by size: 1488 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5185 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5185 SmaI CCC'GGG Cuts at: 1872 2512 1872 Size: 640 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3289 4190 4452 820 Size: 197 926 1346 901 262 1553 Fragments arranged by size: 1553 1346 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2976 493 Size: 2483 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5185 SphI G_CATG'C Cuts at: 1132 2543 1132 Size: 1411 3774 SspI AAT'ATT Cuts at: 5 52 3109 5 Size: 47 3057 2081 Fragments arranged by size: 3057 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5185 StyI C'CwwG_G Cuts at: 513 1065 2204 2624 513 Size: 552 1139 420 3074 Fragments arranged by size: 3074 1139 552 420 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3212 3229 3382 3567 4012 325 Size: 1255 1632 17 153 185 445 1498 Fragments arranged by size: 1632 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3431 5143 96 Size: 274 80 33 51 1443 1454 1712 138 Fragments arranged by size: 1712 1454 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2325 2357 5145 5177 2325 Size: 32 2788 32 2333 Fragments arranged by size: 2788 2333 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5185 XbaI T'CTAG_A Cuts at: 2521 2521 Size: 5185 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5185 XmaI C'CCGG_G Cuts at: 1870 2510 1870 Size: 640 4545 XmnI GAAnn'nnTTC Cuts at: 3314 3314 Size: 5185 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2993 276 Size: 53 83 186 936 1459 2468 Fragments arranged by size: 2468 1459 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI