################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AvrII BanI BbeI BbvCI BclI BfrI BlnI BlpI Bpu1102I BsaHI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FbaI FseI Hin1I HpaI KasI KpnI KspI MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 45 GTCGAC GT^[AC][GT]AC AccIII 83 TCCGGA T^CCGGA ApaI 1941 GGGCCC GGGCC^C AspI 849 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 18 CCCGGG C^[CT]CG[GA]G AviII 917 TGCGCA TGC^GCA BamHI 929 GGATCC G^GATCC BcgI 953 CGACAGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1100 GTATCC GTATCC(6/5) BsaI 2525 GAGACC GGTCTC(1/5) BseAI 83 TCCGGA T^CCGGA BsmI 948 GCATTC GAATGC(1/-1) BsoBI 18 CCCGGG C^[CT]CG[GA]G BspEI 83 TCCGGA T^CCGGA BsrBI 1321 GAGCGG CCGCTC(-3/-3) BsrDI 621 CATTGC GCAATG(2/0) BsrFI 1469 GCCGGT [GA]^CCGG[CT] BstAPI 89 GCAAAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 1469 GCCGGT [GA]^CCGG[CT] CpoI 310 CGGACCG CG^G[AT]CCG DraI 297 TTTAAA TTT^AAA DrdI 36 GACTTCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2338 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 419 GATATC GAT^ATC FspI 917 TGCGCA TGC^GCA HaeII 2322 AGCGCC [GA]GCGC^[CT] HgaI 174 GACGC GACGC(5/10) HindIII 187 AAGCTT A^AGCTT MroI 83 TCCGGA T^CCGGA NspI 94 ACATGC [GA]CATG^[CT] PflFI 849 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC RsrII 310 CGGACCG CG^G[AT]CCG SalI 45 GTCGAC G^TCGAC SmaI 18 CCCGGG CCC^GGG SmlI 1318 CTTGAG C^T[CT][GA]AG StuI 957 AGGCCT AGG^CCT Tth111I 849 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 170 CCAGGACGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 18 CCCGGG C^CCGGG XmaI 18 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 176 CGCG CG^CG 892 CGCG Alw44I 344 GTGCAC G^TGCAC 2174 GTGCAC AocI 924 CCTCAGG CC^T[ACGT]AGG 981 CCTTAGG ApaLI 344 GTGCAC G^TGCAC 2174 GTGCAC Asp700I 777 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2373 GAAACTGTTC BbrPI 703 CACGTG CAC^GTG 2717 CACGTG BglI 1483 GCCAGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1689 GCCTGAGAGGC BglII 389 AGATCT A^GATCT 902 AGATCT BsaAI 703 CACGTG [CT]AC^GT[GA] 2717 CACGTG BsaBI 268 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 752 GATTCTTATC BsaWI 83 TCCGGA [AT]^CCGG[AT] 308 ACCGGA BssSI 208 CACGAG CACGAG(-5/-1) 1561 CACGAG BstUI 176 CGCG CG^CG 892 CGCG BstXI 766 CCAAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1465 CCAAGCCGGTGG Bsu36I 924 CCTCAGG CC^T[ACGT]AGG 981 CCTTAGG Eco81I 924 CCTCAGG CC^T[ACGT]AGG 981 CCTTAGG EcoT14I 734 CCATGG C^C[AT][AT]GG 1028 CCATGG HincII 45 GTCGAC GT[CT]^[GA]AC 2014 GTTGAC HindII 45 GTCGAC GT[CT]^[GA]AC 2014 GTTGAC MamI 268 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 752 GATTCTTATC MvnI 176 CGCG CG^CG 892 CGCG NcoI 734 CCATGG C^CATGG 1028 CCATGG NdeI 325 CATATG CA^TATG 1706 CATATG PflMI 324 CCATATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1611 CCAGAAACTGG PmaCI 703 CACGTG CAC^GTG 2717 CACGTG PmlI 703 CACGTG CAC^GTG 2717 CACGTG PpuMI 1974 AGGACCT [GA]G^G[AT]CC[CT] 2334 GGGACCT SacI 2103 GAGCTC GAGCT^C 2449 GAGCTC StyI 734 CCATGG C^C[AT][AT]GG 1028 CCATGG TfiI 752 GATTC G^A[AT]TC 911 GAATC Van91I 324 CCATATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1611 CCAGAAACTGG XmnI 777 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2373 GAAACTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BalI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PshAI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII