(Circular) (Six-base) MAPSORT of: pFN21AB7309.seq Check: 1518 from: 1 to: 7438 pFN21AB7309 7438 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4471 4471 Size: 7438 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5248 278 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2051 4781 1937 Size: 114 2730 4594 Fragments arranged by size: 4594 2730 114 Acc65I G'GTAC_C Cuts at: 4759 4759 Size: 7438 AclI AA'CG_TT Cuts at: 1563 5565 5938 1563 Size: 4002 373 3063 Fragments arranged by size: 4002 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5501 6885 7153 1632 Size: 3869 1384 268 1917 Fragments arranged by size: 3869 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7241 AflIII A'CryG_T Cuts at: 1203 2707 3582 4721 6337 7314 1203 Size: 1504 875 1139 1616 977 1327 Fragments arranged by size: 1616 1504 1327 1139 977 875 AgeI A'CCGG_T Cuts at: 6269 6269 Size: 7438 AhdI GACnn_n'nnGTC Cuts at: 6167 6167 Size: 7438 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7438 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3627 1184 Size: 2443 4995 AlwNI CAG_nnn'CTG Cuts at: 2075 2576 2974 3607 3622 4381 5181 6753 2075 Size: 501 398 633 15 759 800 1572 2760 Fragments arranged by size: 2760 1572 800 759 633 501 398 15 ApaI G_GGCC'C Cuts at: 3950 3950 Size: 7438 ApaLI G'TGCA_C Cuts at: 2349 4179 5495 6651 2349 Size: 1830 1316 1156 3136 Fragments arranged by size: 3136 1830 1316 1156 ApoI r'AATT_y Cuts at: 1493 2305 2672 4683 4747 4973 5125 7234 1493 Size: 812 367 2011 64 226 152 2109 1697 Fragments arranged by size: 2109 2011 1697 812 367 226 152 64 AseI AT'TA_AT Cuts at: 160 1032 5992 160 Size: 872 4960 1606 Fragments arranged by size: 4960 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 AvaI C'yCGr_G Cuts at: 1870 1943 2023 4763 1870 Size: 73 80 2740 4545 Fragments arranged by size: 4545 2740 80 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4246 4279 4246 Size: 33 7405 BamHI G'GATC_C Cuts at: 2934 4768 5135 2934 Size: 1834 367 5237 Fragments arranged by size: 5237 1834 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4759 6214 618 Size: 325 217 627 2972 1455 1842 Fragments arranged by size: 2972 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3950 4112 4458 4562 4757 1391 Size: 2559 162 346 104 195 4072 Fragments arranged by size: 4072 2559 346 195 162 104 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2045 3579 4449 928 Size: 971 146 1534 870 3917 Fragments arranged by size: 3917 1534 971 870 146 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2947 2981 5629 5663 1903 Size: 34 1010 34 2648 34 3678 Fragments arranged by size: 3678 2648 1010 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3116 5330 6540 3116 Size: 2214 1210 4014 Fragments arranged by size: 4014 2214 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7438 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3494 3700 6049 Size: 107 122 71 631 605 1822 206 2349 Cuts at: 6049 136 Size: 1525 Fragments arranged by size: 2349 1822 1525 631 605 206 122 107 71 BglII A'GATC_T Cuts at: 2394 2907 7433 2394 Size: 513 4526 2399 Fragments arranged by size: 4526 2399 513 BlpI GC'TnA_GC Cuts at: 4855 4855 Size: 7438 Bme1580I G_kGCm'C Cuts at: 1790 2353 3616 3950 4183 4316 5499 6655 1790 Size: 563 1263 334 233 133 1183 1156 2573 Fragments arranged by size: 2573 1263 1183 1156 563 334 233 133 BmgBI CAC'GTC Cuts at: 1318 3585 1318 Size: 2267 5171 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2389 4010 4048 Size: 416 53 83 399 24 972 1621 38 Cuts at: 4048 5169 6127 442 Size: 1121 958 1753 Fragments arranged by size: 1753 1621 1121 972 958 416 399 83 53 38 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7438 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3336 3336 Size: 7438 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2052 2089 2573 3577 3644 3790 4025 4474 Size: 813 37 484 1004 67 146 235 449 Cuts at: 4474 6098 1239 Size: 1624 2579 Fragments arranged by size: 2579 1624 1004 813 484 449 235 146 67 37 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3344 5563 6428 6726 3344 Size: 2219 865 298 4056 Fragments arranged by size: 4056 2219 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4524 6101 882 Size: 617 3025 1577 2219 Fragments arranged by size: 3025 2219 1577 617 BsaAI yAC'GTr Cuts at: 493 1204 2710 4724 5229 493 Size: 711 1506 2014 505 2702 Fragments arranged by size: 2702 2014 1506 711 505 BsaBI GATnn'nnATC Cuts at: 2277 2761 7432 2277 Size: 484 4671 2283 Fragments arranged by size: 4671 2283 484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5245 5627 6262 275 Size: 53 83 186 936 3712 382 635 1451 Fragments arranged by size: 3712 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2088 2313 5871 6269 6543 6690 7101 2088 Size: 225 3558 398 274 147 411 2425 Fragments arranged by size: 3558 2425 411 398 274 225 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2705 2735 1178 Size: 30 165 30 1302 30 5881 Fragments arranged by size: 5881 1302 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4420 4423 4487 1186 Size: 3234 3 64 4137 Fragments arranged by size: 4137 3234 64 3 BseYI C'CCAG_C Cuts at: 3215 4196 4309 6641 7137 3215 Size: 981 113 2332 496 3516 Fragments arranged by size: 3516 2332 981 496 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6865 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4888 5649 6677 1540 Size: 275 186 2887 761 1028 2301 Fragments arranged by size: 2887 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2353 3803 3933 4112 4183 4458 5499 5584 6655 Size: 1450 130 179 71 275 1041 85 1071 Cuts at: 6655 2353 Size: 3136 Fragments arranged by size: 3136 1450 1071 1041 275 179 130 85 71 BsmI GAATG_Cn' Cuts at: 2953 4949 5042 2953 Size: 1996 93 5349 Fragments arranged by size: 5349 1996 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2353 3616 3803 3933 3950 4112 4183 Size: 399 563 1263 187 130 17 162 71 Cuts at: 4183 4316 4458 4562 4757 5499 5584 6655 1391 Size: 133 142 104 195 742 85 1071 2174 Fragments arranged by size: 2174 1263 1071 742 563 399 195 187 162 142 133 130 104 85 71 17 BspEI T'CCGG_A Cuts at: 2088 7101 2088 Size: 5013 2425 BspHI T'CATG_A Cuts at: 5327 7057 5327 Size: 1730 5708 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2522 3991 4471 4793 844 Size: 1678 1469 480 322 3489 Fragments arranged by size: 3489 1678 1469 480 322 BsrBI CCG'CTC Cuts at: 1394 1823 3328 5325 1394 Size: 429 1505 1997 3507 Fragments arranged by size: 3507 1997 1505 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2625 5933 6107 65 Size: 1183 1377 3308 174 1396 Fragments arranged by size: 3308 1396 1377 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3474 6082 6269 1700 Size: 110 1664 2608 187 2869 Fragments arranged by size: 2869 2608 1664 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7438 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7438 BssSI C'ACGA_G Cuts at: 2213 3566 5498 6510 2213 Size: 1353 1932 1012 3141 Fragments arranged by size: 3141 1932 1353 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2100 794 Size: 1306 6132 BstBI TT'CG_AA Cuts at: 6242 6242 Size: 7438 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7438 BstXI CCAn_nnnn'nTGG Cuts at: 2778 3477 2778 Size: 699 6739 BstYI r'GATC_y Cuts at: 1972 2394 2583 2907 2934 4142 4768 5135 5520 Size: 422 189 324 27 1208 626 367 385 Cuts at: 5520 5537 6989 7075 7087 7433 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1208 626 422 385 367 346 324 189 86 27 17 12 Bsu36I CC'TnA_GG Cuts at: 2930 2987 2930 Size: 57 7381 BtgI C'CryG_G Cuts at: 513 1065 2004 2739 3033 3765 513 Size: 552 939 735 294 732 4186 Fragments arranged by size: 4186 939 735 732 552 294 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6699 Fragments arranged by size: 6699 683 56 BtsI GCAGTG_nn' Cuts at: 4183 4211 4558 4954 5748 5768 4183 Size: 28 347 396 794 20 5853 Fragments arranged by size: 5853 794 396 347 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7438 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7403 DraI TTT'AAA Cuts at: 2304 4742 2304 Size: 2438 5000 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2047 6445 809 Size: 507 731 4398 1802 Fragments arranged by size: 4398 1802 731 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2362 2589 2820 Size: 54 1425 50 275 18 532 227 231 Cuts at: 2820 3030 3762 4885 5774 7403 8 Size: 210 732 1123 889 1629 43 Fragments arranged by size: 1629 1425 1123 889 732 532 275 231 227 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4885 1537 Size: 275 3073 4090 Fragments arranged by size: 4090 3073 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2493 3714 3791 3921 4399 4539 4567 5368 Size: 1140 1221 77 130 478 140 28 801 Cuts at: 5368 1353 Size: 3423 Fragments arranged by size: 3423 1221 1140 801 478 140 130 77 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3007 3418 3436 3868 6025 6399 Size: 1064 138 1418 411 18 432 2157 374 Cuts at: 6399 6545 387 Size: 146 1280 Fragments arranged by size: 2157 1418 1280 1064 432 411 374 146 138 18 EcoICRI GAG'CTC Cuts at: 4110 4456 4110 Size: 346 7092 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2052 2089 2573 3577 3644 3790 4025 Size: 393 420 37 484 1004 67 146 235 Cuts at: 4025 4474 5501 6098 6885 7153 1239 Size: 449 1027 597 787 268 1524 Fragments arranged by size: 1524 1027 1004 787 597 484 449 420 393 268 235 146 67 37 EcoNI CCTnn'n_nnAGG Cuts at: 4347 4347 Size: 7438 EcoO109I rG'GnC_Cy Cuts at: 2131 3946 3947 3980 4340 2131 Size: 1815 1 33 360 5229 Fragments arranged by size: 5229 1815 360 33 1 EcoRI G'AATT_C Cuts at: 4747 5125 4747 Size: 378 7060 EcoRV GAT'ATC Cuts at: 2426 2426 Size: 7438 FspI TGC'GCA Cuts at: 2924 5944 2924 Size: 3020 4418 FspAI rTGC'GCAy Cuts at: 2924 2924 Size: 7438 HaeII r_GCGC'y Cuts at: 1678 4331 6585 1678 Size: 2653 2254 2531 Fragments arranged by size: 2653 2531 2254 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2103 2135 3643 3675 3689 Size: 32 135 32 531 32 1508 32 14 Cuts at: 3689 3721 4648 4680 6079 6111 6153 6185 1373 Size: 32 927 32 1399 32 42 32 2626 Fragments arranged by size: 2626 1508 1399 927 531 135 42 32 32 32 32 32 32 32 32 14 HincII GTy'rAC Cuts at: 669 1657 1938 2052 4021 4782 5028 669 Size: 988 281 114 1969 761 246 3079 Fragments arranged by size: 3079 1969 988 761 281 246 114 HindIII A'AGCT_T Cuts at: 748 2192 748 Size: 1444 5994 HpaI GTT'AAC Cuts at: 5028 5028 Size: 7438 KpnI G_GTAC'C Cuts at: 4763 4763 Size: 7438 MfeI C'AATT_G Cuts at: 5037 5037 Size: 7438 MluI A'CGCG_T Cuts at: 7314 7314 Size: 7438 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2886 3483 3540 4716 6552 6736 1305 Size: 1581 597 57 1176 1836 184 2007 Fragments arranged by size: 2007 1836 1581 1176 597 184 57 MscI TGG'CCA Cuts at: 10 64 1489 1832 2364 2591 2822 3032 10 Size: 54 1425 343 532 227 231 210 4416 Fragments arranged by size: 4416 1425 532 343 231 227 210 54 MslI CAynn'nnrTG Cuts at: 518 1166 3121 3137 3609 4028 5396 5755 5914 Size: 648 1955 16 472 419 1368 359 159 Cuts at: 5914 518 Size: 2042 Fragments arranged by size: 2042 1955 1368 648 472 419 359 159 16 MspA1I CmG'CkG Cuts at: 1606 3574 3760 3828 3865 4229 4854 5531 6679 Size: 1968 186 68 37 364 625 677 1148 Cuts at: 6679 6924 1606 Size: 245 2120 Fragments arranged by size: 2120 1968 1148 677 625 364 245 186 68 37 NcoI C'CATG_G Cuts at: 513 1065 2004 2739 3033 513 Size: 552 939 735 294 4918 Fragments arranged by size: 4918 939 735 552 294 NdeI CA'TA_TG Cuts at: 387 2331 3712 387 Size: 1944 1381 4113 Fragments arranged by size: 4113 1944 1381 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7438 NotI GC'GGCC_GC Cuts at: 4885 4885 Size: 7438 NspI r_CATG'y Cuts at: 1132 1229 2103 4796 6341 1132 Size: 97 874 2693 1545 2229 Fragments arranged by size: 2693 2229 1545 874 97 PasI CC'CwG_GG Cuts at: 1348 3917 4291 4460 1348 Size: 2569 374 169 4326 Fragments arranged by size: 4326 2569 374 169 PciI A'CATG_T Cuts at: 6337 6337 Size: 7438 PflMI CCAn_nnn'nTGG Cuts at: 1108 2335 3622 1108 Size: 1227 1287 4924 Fragments arranged by size: 4924 1287 1227 PfoI T'CCnGG_A Cuts at: 2105 2223 2281 2105 Size: 118 58 7262 Fragments arranged by size: 7262 118 58 PmeI GTTT'AAAC Cuts at: 4742 4742 Size: 7438 PmlI CAC'GTG Cuts at: 2710 4724 2710 Size: 2014 5424 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5507 5539 7020 7052 5507 Size: 32 1481 32 5893 Fragments arranged by size: 5893 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3980 4340 3980 Size: 360 7078 PshAI GACnn'nnGTC Cuts at: 1613 3779 4581 4638 1613 Size: 2166 802 57 4413 Fragments arranged by size: 4413 2166 802 57 PsiI TTA'TAA Cuts at: 5008 5008 Size: 7438 PspOMI G'GGCC_C Cuts at: 3946 3946 Size: 7438 PstI C_TGCA'G Cuts at: 830 2661 2880 2970 4790 830 Size: 1831 219 90 1820 3478 Fragments arranged by size: 3478 1831 1820 219 90 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7438 PvuII CAG'CTG Cuts at: 3574 3760 3828 4229 3574 Size: 186 68 401 6783 Fragments arranged by size: 6783 401 186 68 RsrII CG'GwC_CG Cuts at: 2316 2316 Size: 7438 SacI G_AGCT'C Cuts at: 4112 4458 4112 Size: 346 7092 SalI G'TCGA_C Cuts at: 1936 2050 4780 1936 Size: 114 2730 4594 Fragments arranged by size: 4594 2730 114 SapI GCTCTTCn'nnn_ Cuts at: 3791 3921 4567 3791 Size: 130 646 6662 Fragments arranged by size: 6662 646 130 SbfI CC_TGCA'GG Cuts at: 4790 4790 Size: 7438 ScaI AGT'ACT Cuts at: 5686 7398 5686 Size: 1712 5726 SfcI C'TryA_G Cuts at: 826 1045 2657 2876 2966 4591 4786 5921 6602 Size: 219 1612 219 90 1625 195 1135 681 Cuts at: 6602 6793 826 Size: 191 1471 Fragments arranged by size: 1625 1612 1471 1135 681 219 219 195 191 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7438 SmaI CCC'GGG Cuts at: 1872 2025 4765 1872 Size: 153 2740 4545 Fragments arranged by size: 4545 2740 153 SmlI C'TyrA_G Cuts at: 820 1017 1943 3323 5542 6443 6705 820 Size: 197 926 1380 2219 901 262 1553 Fragments arranged by size: 2219 1553 1380 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5229 493 Size: 4736 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7438 SphI G_CATG'C Cuts at: 1132 4796 1132 Size: 3664 3774 SspI AAT'ATT Cuts at: 5 52 5362 5 Size: 47 5310 2081 Fragments arranged by size: 5310 2081 47 StuI AGG'CCT Cuts at: 1517 2964 1517 Size: 1447 5991 StyI C'CwwG_G Cuts at: 513 1065 2004 2739 3033 4877 513 Size: 552 939 735 294 1844 3074 Fragments arranged by size: 3074 1844 939 735 552 294 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3684 3791 5465 5482 5635 5820 6265 Size: 1255 2104 107 1674 17 153 185 445 Cuts at: 6265 325 Size: 1498 Fragments arranged by size: 2104 1674 1498 1255 445 185 153 107 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5684 7396 96 Size: 274 80 33 51 1443 3707 1712 138 Fragments arranged by size: 3707 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2330 2362 2705 2737 4084 4116 7398 7430 2330 Size: 32 343 32 1347 32 3282 32 2338 Fragments arranged by size: 3282 2338 1347 343 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2857 1316 Size: 1541 5897 XbaI T'CTAG_A Cuts at: 4774 4774 Size: 7438 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2182 2182 Size: 7438 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7438 XmaI C'CCGG_G Cuts at: 1870 2023 4763 1870 Size: 153 2740 4545 Fragments arranged by size: 4545 2740 153 XmnI GAAnn'nnTTC Cuts at: 2786 4382 5567 2786 Size: 1596 1185 4657 Fragments arranged by size: 4657 1596 1185 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5246 276 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI SacII SanDI SexAI SfiI SfoI SrfI SwaI