(Linear) (Six-base) MAPSORT of: KIBB7309.seq Check: 3128 from: 1 to: 2733 KIBB7309.orf With 182 enzymes: SgfI * December 5, 2014 12:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2466 2733 Size: 2466 267 AccI GT'mk_AC Cuts at: 0 46 2733 Size: 46 2687 AflIII A'CryG_T Cuts at: 0 702 1577 2716 2733 Size: 702 875 1139 17 Fragments arranged by size: 1139 875 702 17 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1622 2733 Size: 1622 1111 AlwNI CAG_nnn'CTG Cuts at: 0 70 571 969 1602 1617 2376 2733 Size: 70 501 398 633 15 759 357 Fragments arranged by size: 759 633 501 398 357 70 15 ApaI G_GGCC'C Cuts at: 0 1945 2733 Size: 1945 788 ApaLI G'TGCA_C Cuts at: 0 344 2174 2733 Size: 344 1830 559 Fragments arranged by size: 1830 559 344 ApoI r'AATT_y Cuts at: 0 300 667 2678 2733 Size: 300 367 2011 55 Fragments arranged by size: 2011 367 300 55 AvaI C'yCGr_G Cuts at: 0 18 2733 Size: 18 2715 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2241 2274 2733 Size: 2241 33 459 Fragments arranged by size: 2241 459 33 BamHI G'GATC_C Cuts at: 0 929 2733 Size: 929 1804 BanII G_rGCy'C Cuts at: 0 1945 2107 2453 2557 2733 Size: 1945 162 346 104 176 Fragments arranged by size: 1945 346 176 162 104 BbsI GAAGACnn'nnnn_ Cuts at: 0 40 1574 2444 2733 Size: 40 1534 870 289 Fragments arranged by size: 1534 870 289 40 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 942 976 2733 Size: 942 34 1757 Fragments arranged by size: 1757 942 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1111 2733 Size: 1111 1622 BglI GCCn_nnn'nGGC Cuts at: 0 1489 1695 2733 Size: 1489 206 1038 Fragments arranged by size: 1489 1038 206 BglII A'GATC_T Cuts at: 0 389 902 2733 Size: 389 513 1831 Fragments arranged by size: 1831 513 389 Bme1580I G_kGCm'C Cuts at: 0 348 1611 1945 2178 2311 2733 Size: 348 1263 334 233 133 422 Fragments arranged by size: 1263 422 348 334 233 133 BmgBI CAC'GTC Cuts at: 0 1580 2733 Size: 1580 1153 BmrI ACTGGGnnnn_n' Cuts at: 0 384 2005 2043 2733 Size: 384 1621 38 690 Fragments arranged by size: 1621 690 384 38 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1331 2733 Size: 1331 1402 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 47 84 568 1572 1639 1785 2020 2469 Size: 47 37 484 1004 67 146 235 449 Cuts at: 2469 2733 Size: 264 Fragments arranged by size: 1004 484 449 264 235 146 67 47 37 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1339 2733 Size: 1339 1394 BsaI GGTCTCn'nnnn_ Cuts at: 0 2519 2733 Size: 2519 214 BsaAI yAC'GTr Cuts at: 0 705 2719 2733 Size: 705 2014 14 Fragments arranged by size: 2014 705 14 BsaBI GATnn'nnATC Cuts at: 0 272 756 2733 Size: 272 484 1977 Fragments arranged by size: 1977 484 272 BsaWI w'CCGG_w Cuts at: 0 83 308 2733 Size: 83 225 2425 Fragments arranged by size: 2425 225 83 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 700 730 2733 Size: 700 30 2003 Fragments arranged by size: 2003 700 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2415 2418 2482 2733 Size: 2415 3 64 251 Fragments arranged by size: 2415 251 64 3 BseYI C'CCAG_C Cuts at: 0 1210 2191 2304 2733 Size: 1210 981 113 429 Fragments arranged by size: 1210 981 429 113 BsiHKAI G_wGCw'C Cuts at: 0 348 1798 1928 2107 2178 2453 2733 Size: 348 1450 130 179 71 275 280 Fragments arranged by size: 1450 348 280 275 179 130 71 BsmI GAATG_Cn' Cuts at: 0 948 2733 Size: 948 1785 Bsp1286I G_dGCh'C Cuts at: 0 348 1611 1798 1928 1945 2107 2178 2311 Size: 348 1263 187 130 17 162 71 133 Cuts at: 2311 2453 2557 2733 Size: 142 104 176 Fragments arranged by size: 1263 348 187 176 162 142 133 130 104 71 17 BspEI T'CCGG_A Cuts at: 0 83 2733 Size: 83 2650 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 517 1986 2466 2733 Size: 517 1469 480 267 Fragments arranged by size: 1469 517 480 267 BsrBI CCG'CTC Cuts at: 0 1323 2733 Size: 1323 1410 BsrDI GCAATG_nn' Cuts at: 0 620 2733 Size: 620 2113 BsrFI r'CCGG_y Cuts at: 0 1469 2733 Size: 1469 1264 BssSI C'ACGA_G Cuts at: 0 208 1561 2733 Size: 208 1353 1172 Fragments arranged by size: 1353 1172 208 BstAPI GCAn_nnn'nTGC Cuts at: 0 95 2733 Size: 95 2638 BstXI CCAn_nnnn'nTGG Cuts at: 0 773 1472 2733 Size: 773 699 1261 Fragments arranged by size: 1261 773 699 BstYI r'GATC_y Cuts at: 0 389 578 902 929 2137 2733 Size: 389 189 324 27 1208 596 Fragments arranged by size: 1208 596 389 324 189 27 Bsu36I CC'TnA_GG Cuts at: 0 925 982 2733 Size: 925 57 1751 Fragments arranged by size: 1751 925 57 BtgI C'CryG_G Cuts at: 0 734 1028 1760 2733 Size: 734 294 732 973 Fragments arranged by size: 973 734 732 294 BtsI GCAGTG_nn' Cuts at: 0 2178 2206 2553 2733 Size: 2178 28 347 180 Fragments arranged by size: 2178 347 180 28 DraI TTT'AAA Cuts at: 0 299 2733 Size: 299 2434 DrdI GACnn_nn'nnGTC Cuts at: 0 42 2733 Size: 42 2691 EaeI y'GGCC_r Cuts at: 0 357 584 815 1025 1757 2733 Size: 357 227 231 210 732 976 Fragments arranged by size: 976 732 357 231 227 210 EarI CTCTTCn'nnn_ Cuts at: 0 488 1709 1786 1916 2394 2534 2562 2733 Size: 488 1221 77 130 478 140 28 171 Fragments arranged by size: 1221 488 478 171 140 130 77 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1002 1413 1431 1863 2733 Size: 1002 411 18 432 870 Fragments arranged by size: 1002 870 432 411 18 EcoICRI GAG'CTC Cuts at: 0 2105 2451 2733 Size: 2105 346 282 Fragments arranged by size: 2105 346 282 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 47 84 568 1572 1639 1785 2020 2469 Size: 47 37 484 1004 67 146 235 449 Cuts at: 2469 2733 Size: 264 Fragments arranged by size: 1004 484 449 264 235 146 67 47 37 EcoNI CCTnn'n_nnAGG Cuts at: 0 2342 2733 Size: 2342 391 EcoO109I rG'GnC_Cy Cuts at: 0 126 1941 1942 1975 2335 2733 Size: 126 1815 1 33 360 398 Fragments arranged by size: 1815 398 360 126 33 1 EcoRV GAT'ATC Cuts at: 0 421 2733 Size: 421 2312 FspI TGC'GCA Cuts at: 0 919 2733 Size: 919 1814 FspAI rTGC'GCAy Cuts at: 0 919 2733 Size: 919 1814 HaeII r_GCGC'y Cuts at: 0 2326 2733 Size: 2326 407 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 98 130 1638 1670 1684 1716 2643 2675 Size: 98 32 1508 32 14 32 927 32 Cuts at: 2675 2733 Size: 58 Fragments arranged by size: 1508 927 98 58 32 32 32 32 14 HincII GTy'rAC Cuts at: 0 47 2016 2733 Size: 47 1969 717 Fragments arranged by size: 1969 717 47 HindIII A'AGCT_T Cuts at: 0 187 2733 Size: 187 2546 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 881 1478 1535 2711 2733 Size: 881 597 57 1176 22 Fragments arranged by size: 1176 881 597 57 22 MscI TGG'CCA Cuts at: 0 359 586 817 1027 2733 Size: 359 227 231 210 1706 Fragments arranged by size: 1706 359 231 227 210 MslI CAynn'nnrTG Cuts at: 0 1116 1132 1604 2023 2733 Size: 1116 16 472 419 710 Fragments arranged by size: 1116 710 472 419 16 MspA1I CmG'CkG Cuts at: 0 1569 1755 1823 1860 2224 2733 Size: 1569 186 68 37 364 509 Fragments arranged by size: 1569 509 364 186 68 37 NcoI C'CATG_G Cuts at: 0 734 1028 2733 Size: 734 294 1705 Fragments arranged by size: 1705 734 294 NdeI CA'TA_TG Cuts at: 0 326 1707 2733 Size: 326 1381 1026 Fragments arranged by size: 1381 1026 326 NspI r_CATG'y Cuts at: 0 98 2733 Size: 98 2635 PasI CC'CwG_GG Cuts at: 0 1912 2286 2455 2733 Size: 1912 374 169 278 Fragments arranged by size: 1912 374 278 169 PflMI CCAn_nnn'nTGG Cuts at: 0 330 1617 2733 Size: 330 1287 1116 Fragments arranged by size: 1287 1116 330 PfoI T'CCnGG_A Cuts at: 0 100 218 276 2733 Size: 100 118 58 2457 Fragments arranged by size: 2457 118 100 58 PmlI CAC'GTG Cuts at: 0 705 2719 2733 Size: 705 2014 14 Fragments arranged by size: 2014 705 14 PpuMI rG'GwC_Cy Cuts at: 0 1975 2335 2733 Size: 1975 360 398 Fragments arranged by size: 1975 398 360 PshAI GACnn'nnGTC Cuts at: 0 1774 2576 2633 2733 Size: 1774 802 57 100 Fragments arranged by size: 1774 802 100 57 PspOMI G'GGCC_C Cuts at: 0 1941 2733 Size: 1941 792 PstI C_TGCA'G Cuts at: 0 656 875 965 2733 Size: 656 219 90 1768 Fragments arranged by size: 1768 656 219 90 PvuII CAG'CTG Cuts at: 0 1569 1755 1823 2224 2733 Size: 1569 186 68 401 509 Fragments arranged by size: 1569 509 401 186 68 RsrII CG'GwC_CG Cuts at: 0 311 2733 Size: 311 2422 SacI G_AGCT'C Cuts at: 0 2107 2453 2733 Size: 2107 346 280 Fragments arranged by size: 2107 346 280 SalI G'TCGA_C Cuts at: 0 45 2733 Size: 45 2688 SapI GCTCTTCn'nnn_ Cuts at: 0 1786 1916 2562 2733 Size: 1786 130 646 171 Fragments arranged by size: 1786 646 171 130 SfcI C'TryA_G Cuts at: 0 652 871 961 2586 2733 Size: 652 219 90 1625 147 Fragments arranged by size: 1625 652 219 147 90 SmaI CCC'GGG Cuts at: 0 20 2733 Size: 20 2713 SmlI C'TyrA_G Cuts at: 0 1318 2733 Size: 1318 1415 StuI AGG'CCT Cuts at: 0 959 2733 Size: 959 1774 StyI C'CwwG_G Cuts at: 0 734 1028 2733 Size: 734 294 1705 Fragments arranged by size: 1705 734 294 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1679 1786 2733 Size: 1679 107 947 Fragments arranged by size: 1679 947 107 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 325 357 700 732 2079 2111 2733 Size: 325 32 343 32 1347 32 622 Fragments arranged by size: 1347 622 343 325 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 852 2733 Size: 852 1881 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 177 2733 Size: 177 2556 XmaI C'CCGG_G Cuts at: 0 18 2733 Size: 18 2715 XmnI GAAnn'nnTTC Cuts at: 0 781 2377 2733 Size: 781 1596 356 Fragments arranged by size: 1596 781 356 Enzymes that do cut: AarI AccI AflIII AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanII BbsI BcgI BciVI BglI BglII Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI FspAI HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NdeI NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII RsrII SacI SalI SapI SfcI SmaI SmlI StuI StyI TaqII TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AloI AscI AseI AsiSI AvrII BanI BbeI BbvCI BclI BfrBI BlpI BmtI Bpu10I BsaHI BsgI BsiEI BsiWI BsmBI BspHI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DraIII EagI EcoRI FalI FseI HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI SacII SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI XhoI ZraI