################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FseI FspI HincII HindII HpaI KpnI Ksp632I KspI MfeI MluI MluNI MroI MscI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### ApaI 101 GGGCCC GGGCC^C Asp700I 658 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 321 GGATCC G^GATCC BanI 95 GGCGCC G^G[CT][GA]CC BanII 101 GGGCCC G[GA]GC[CT]^C BbeI 95 GGCGCC GGCGC^C BclI 626 TGATCA T^GATCA BfaI 266 CTAG C^TAG BglII 255 AGATCT A^GATCT BmyI 101 GGGCCC G[AGT]GC[ACT]^C BsaBI 178 GATTTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 51 ACCGGA [AT]^CCGG[AT] BsgI 251 GTGCAG GTGCAG(16/14) BsmI 827 GCATTC GAATGC(1/-1) Bsp1286I 101 GGGCCC G[AGT]GC[ACT]^C Bsp1407I 429 TGTACA T^GTACA BsrGI 429 TGTACA T^GTACA BssHII 168 GCGCGC G^CGCGC DraII 365 GGGACCC [GA]G^G[ACGT]CC[CT] DrdI 367 GACCCCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 282 CCTCCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 365 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 745 GAATTC G^AATTC EcoT22I 670 ATGCAT ATGCA^T FbaI 626 TGATCA T^GATCA HgaI 116 GCGTC GACGC(5/10) HindIII 693 AAGCTT A^AGCTT KasI 95 GGCGCC G^GCGCC MaeI 266 CTAG C^TAG MaeII 392 ACGT A^CGT MaeIII 204 GTTAC ^GT[ACGT]AC MamI 178 GATTTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 653 TTAA T^TAA MslI 667 CACATGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 95 GGCGCC GG^CGCC NsiI 670 ATGCAT ATGCA^T NspI 668 ACATGC [GA]CATG^[CT] PpuMI 365 GGGACCC [GA]G^G[AT]CC[CT] PshAI 760 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 70 CTGCAG CTGCA^G SfaNI 578 GCATC GCATC(5/9) SfcI 70 CTGCAG C^T[GA][CT]AG SfoI 95 GGCGCC GGC^GCC SspBI 429 TGTACA T^GTACA Tru9I 653 TTAA T^TAA XmnI 658 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 745 GAATTC [GA]^AATT[CT] 780 AAATTT AcyI 95 GGCGCC G[GA]^CG[CT]C 115 GGCGTC AfaI 430 GTAC GT^AC 544 GTAC ApoI 745 GAATTC [GA]^AATT[CT] 780 AAATTT AvaI 104 CCCGGG C^[CT]CG[GA]G 173 CCCGGG BsaHI 95 GGCGCC G[GA]^CG[CT]C 115 GGCGTC BsmFI 365 GGGAC GGGAC(10/14) 118 GTCCC BsoBI 104 CCCGGG C^[CT]CG[GA]G 173 CCCGGG BsrBI 121 CCGCTC CCGCTC(-3/-3) 136 CCGCTC DsaI 300 CCATGG C^C[GA][CT]GG 752 CCATGG EcoT14I 300 CCATGG C^C[AT][AT]GG 752 CCATGG FauI 120 CCCGC CCCGC(4/6) 28 GCGGG HaeII 87 GGCGCT [GA]GCGC^[CT] 95 GGCGCC Hin1I 95 GGCGCC G[GA]^CG[CT]C 115 GGCGTC HphI 739 GGTGA GGTGA(8/7) 140 TCACC MspA1I 130 CCGCTG C[AC]G^C[GT]G 448 CAGCGG NcoI 300 CCATGG C^CATGG 752 CCATGG PleI 145 GACTC GAGTC(4/5) 305 GAGTC RsaI 430 GTAC GT^AC 544 GTAC SmaI 104 CCCGGG CCC^GGG 173 CCCGGG StyI 300 CCATGG C^C[AT][AT]GG 752 CCATGG TaqI 182 TCGA T^CGA 399 TCGA TspRI 327 CACTG CA[GC]TG[ACGT][ACGT]^ 361 CACTG TthHB8I 182 TCGA T^CGA 399 TCGA XmaCI 104 CCCGGG C^CCGGG 173 CCCGGG XmaI 104 CCCGGG C^CCGGG 173 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AccII AciI AluI AlwI AvaII BbvI BcnI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MflI MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TfiI TseI Tsp509I XhoII