(Circular) (Six-base) MAPSORT of: pFN21AE6262.seq Check: 743 from: 1 to: 5539 pFN21AE6262 5539 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3349 278 Size: 53 83 186 936 1813 2468 Fragments arranged by size: 2468 1813 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2882 1937 Size: 945 4594 Acc65I G'GTAC_C Cuts at: 2860 2860 Size: 5539 AclI AA'CG_TT Cuts at: 1563 3666 4039 1563 Size: 2103 373 3063 Fragments arranged by size: 3063 2103 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2690 2823 3602 4986 5254 1632 Size: 1058 133 779 1384 268 1917 Fragments arranged by size: 1917 1384 1058 779 268 133 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5342 AflIII A'CryG_T Cuts at: 1203 4438 5415 1203 Size: 3235 977 1327 Fragments arranged by size: 3235 1327 977 AgeI A'CCGG_T Cuts at: 4370 4370 Size: 5539 AhdI GACnn_n'nnGTC Cuts at: 4268 4268 Size: 5539 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5539 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5539 AlwNI CAG_nnn'CTG Cuts at: 3282 4854 3282 Size: 1572 3967 ApaI G_GGCC'C Cuts at: 2110 2110 Size: 5539 ApaLI G'TGCA_C Cuts at: 3596 4752 3596 Size: 1156 4383 ApoI r'AATT_y Cuts at: 1493 2750 2785 2848 3074 3226 5335 1493 Size: 1257 35 63 226 152 2109 1697 Fragments arranged by size: 2109 1697 1257 226 152 63 35 AseI AT'TA_AT Cuts at: 160 1032 4093 160 Size: 872 3061 1606 Fragments arranged by size: 3061 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 AvaI C'yCGr_G Cuts at: 1870 1943 2109 2178 2864 1870 Size: 73 166 69 686 4545 Fragments arranged by size: 4545 686 166 73 69 BamHI G'GATC_C Cuts at: 2326 2869 3236 2326 Size: 543 367 4629 Fragments arranged by size: 4629 543 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2100 2860 4315 618 Size: 325 217 627 313 760 1455 1842 Fragments arranged by size: 1842 1455 760 627 325 313 217 BanII G_rGCy'C Cuts at: 1391 2110 2858 1391 Size: 719 748 4072 Fragments arranged by size: 4072 748 719 BbeI G_GCGC'C Cuts at: 2104 2104 Size: 5539 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4568 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3730 3764 1903 Size: 34 1793 34 3678 Fragments arranged by size: 3678 1793 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3431 4641 3431 Size: 1210 4329 BclI T'GATC_A Cuts at: 1548 2631 1548 Size: 1083 4456 BfrBI ATG'CAT Cuts at: 2677 2677 Size: 5539 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4150 136 Size: 107 122 71 631 605 2478 1525 Fragments arranged by size: 2478 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2260 5534 2260 Size: 3274 2265 BlpI GC'TnA_GC Cuts at: 2956 2956 Size: 5539 Bme1580I G_kGCm'C Cuts at: 1790 2110 3600 4756 1790 Size: 320 1490 1156 2573 Fragments arranged by size: 2573 1490 1156 320 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5539 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2377 3270 4228 Size: 416 53 83 399 24 960 893 958 Cuts at: 4228 442 Size: 1753 Fragments arranged by size: 1753 960 958 893 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5539 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4199 1239 Size: 2960 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3664 4529 4827 3664 Size: 865 298 4376 Fragments arranged by size: 4376 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4202 882 Size: 617 2703 2219 Fragments arranged by size: 2703 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3330 493 Size: 711 2126 2702 Fragments arranged by size: 2702 2126 711 BsaBI GATnn'nnATC Cuts at: 2187 5533 2187 Size: 3346 2193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2101 2121 3346 3728 Size: 53 83 186 936 568 20 1225 382 Cuts at: 3728 4363 275 Size: 635 1451 Fragments arranged by size: 1451 1225 936 635 568 382 186 83 53 20 BsaWI w'CCGG_w Cuts at: 2056 3972 4370 4644 4791 5202 2056 Size: 1916 398 274 147 411 2393 Fragments arranged by size: 2393 1916 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5314 Fragments arranged by size: 5314 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5539 BseYI C'CCAG_C Cuts at: 2022 4742 5238 2022 Size: 2720 496 2323 Fragments arranged by size: 2720 2323 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2277 1163 Size: 573 541 4425 Fragments arranged by size: 4425 573 541 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2989 3750 4778 1540 Size: 275 186 988 761 1028 2301 Fragments arranged by size: 2301 1028 988 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3600 3685 4756 3600 Size: 85 1071 4383 Fragments arranged by size: 4383 1071 85 BsmI GAATG_Cn' Cuts at: 2832 3050 3143 2832 Size: 218 93 5228 Fragments arranged by size: 5228 218 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2110 2858 3600 3685 4756 1391 Size: 399 320 748 742 85 1071 2174 Fragments arranged by size: 2174 1071 748 742 399 320 85 BspEI T'CCGG_A Cuts at: 5202 5202 Size: 5539 BspHI T'CATG_A Cuts at: 3428 5158 3428 Size: 1730 3809 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2894 844 Size: 2050 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2128 2143 3426 1394 Size: 429 305 15 1283 3507 Fragments arranged by size: 3507 1283 429 305 15 BsrDI GCAATG_nn' Cuts at: 65 1248 4034 4208 65 Size: 1183 2786 174 1396 Fragments arranged by size: 2786 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4183 4370 1700 Size: 110 2373 187 2869 Fragments arranged by size: 2869 2373 187 110 BsrGI T'GTAC_A Cuts at: 96 2434 96 Size: 2338 3201 BssHII G'CGCG_C Cuts at: 1925 2173 1925 Size: 248 5291 BssSI C'ACGA_G Cuts at: 3599 4611 3599 Size: 1012 4527 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5539 BstBI TT'CG_AA Cuts at: 4343 4343 Size: 5539 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5539 BstYI r'GATC_y Cuts at: 1972 2260 2318 2326 2869 3236 3621 3638 5090 Size: 288 58 8 543 367 385 17 1452 Cuts at: 5090 5176 5188 5534 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 543 385 367 346 288 86 58 17 12 8 BtgI C'CryG_G Cuts at: 513 1065 2004 2305 2757 513 Size: 552 939 301 452 3295 Fragments arranged by size: 3295 939 552 452 301 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4800 Fragments arranged by size: 4800 683 56 BtsI GCAGTG_nn' Cuts at: 3055 3849 3869 3055 Size: 794 20 4725 Fragments arranged by size: 4725 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5539 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5504 DraI TTT'AAA Cuts at: 2843 2843 Size: 5539 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2378 4546 809 Size: 507 1062 2168 1802 Fragments arranged by size: 2168 1802 1062 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2986 3875 5504 Size: 54 1425 50 275 18 1156 889 1629 Cuts at: 5504 8 Size: 43 Fragments arranged by size: 1629 1425 1156 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2986 1537 Size: 275 1174 4090 Fragments arranged by size: 4090 1174 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3469 1353 Size: 2116 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4126 4500 4646 387 Size: 1064 138 2537 374 146 1280 Fragments arranged by size: 2537 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2690 2823 3602 4199 4986 5254 1239 Size: 393 1058 133 779 597 787 268 1524 Fragments arranged by size: 1524 1058 787 779 597 393 268 133 EcoNI CCTnn'n_nnAGG Cuts at: 2291 2291 Size: 5539 EcoO109I rG'GnC_Cy Cuts at: 2371 2371 Size: 5539 EcoRI G'AATT_C Cuts at: 2750 2848 3226 2750 Size: 98 378 5063 Fragments arranged by size: 5063 378 98 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2491 2491 Size: 5539 FspI TGC'GCA Cuts at: 4045 4045 Size: 5539 HaeII r_GCGC'y Cuts at: 1678 2096 2104 4686 1678 Size: 418 8 2582 2531 Fragments arranged by size: 2582 2531 418 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 4180 4212 4254 Size: 32 135 32 431 32 2145 32 42 Cuts at: 4254 4286 1373 Size: 32 2626 Fragments arranged by size: 2626 2145 431 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2883 3129 669 Size: 988 281 945 246 3079 Fragments arranged by size: 3079 988 945 281 246 HindIII A'AGCT_T Cuts at: 748 2698 748 Size: 1950 3589 HpaI GTT'AAC Cuts at: 3129 3129 Size: 5539 KasI G'GCGC_C Cuts at: 2100 2100 Size: 5539 KpnI G_GTAC'C Cuts at: 2864 2864 Size: 5539 MfeI C'AATT_G Cuts at: 3138 3138 Size: 5539 MluI A'CGCG_T Cuts at: 5415 5415 Size: 5539 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4653 4837 1305 Size: 3348 184 2007 Fragments arranged by size: 3348 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3717 Fragments arranged by size: 3717 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2676 3497 3856 4015 518 Size: 648 1510 821 359 159 2042 Fragments arranged by size: 2042 1510 821 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2455 2955 3632 4780 5025 1606 Size: 531 318 500 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 531 500 318 245 NarI GG'CG_CC Cuts at: 2101 2101 Size: 5539 NcoI C'CATG_G Cuts at: 513 1065 2004 2305 2757 513 Size: 552 939 301 452 3295 Fragments arranged by size: 3295 939 552 452 301 NdeI CA'TA_TG Cuts at: 387 387 Size: 5539 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5539 NotI GC'GGCC_GC Cuts at: 2986 2986 Size: 5539 NsiI A_TGCA'T Cuts at: 2679 2679 Size: 5539 NspI r_CATG'y Cuts at: 1132 1229 2677 2897 4442 1132 Size: 97 1448 220 1545 2229 Fragments arranged by size: 2229 1545 1448 220 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5539 PciI A'CATG_T Cuts at: 4438 4438 Size: 5539 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5539 PmeI GTTT'AAAC Cuts at: 2843 2843 Size: 5539 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3608 3640 5121 5153 3608 Size: 32 1481 32 3994 Fragments arranged by size: 3994 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2371 2371 Size: 5539 PshAI GACnn'nnGTC Cuts at: 1613 2769 1613 Size: 1156 4383 PsiI TTA'TAA Cuts at: 2226 2792 3109 2226 Size: 566 317 4656 Fragments arranged by size: 4656 566 317 PspOMI G'GGCC_C Cuts at: 2106 2106 Size: 5539 PstI C_TGCA'G Cuts at: 830 2079 2891 830 Size: 1249 812 3478 Fragments arranged by size: 3478 1249 812 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5539 SalI G'TCGA_C Cuts at: 1936 2881 1936 Size: 945 4594 SanDI GG'GwC_CC Cuts at: 2371 2371 Size: 5539 SbfI CC_TGCA'GG Cuts at: 2891 2891 Size: 5539 ScaI AGT'ACT Cuts at: 3787 5499 3787 Size: 1712 3827 SfcI C'TryA_G Cuts at: 826 1045 2075 2887 4022 4703 4894 826 Size: 219 1030 812 1135 681 191 1471 Fragments arranged by size: 1471 1135 1030 812 681 219 191 SfoI GGC'GCC Cuts at: 2102 2102 Size: 5539 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5539 SmaI CCC'GGG Cuts at: 1872 2111 2180 2866 1872 Size: 239 69 686 4545 Fragments arranged by size: 4545 686 239 69 SmlI C'TyrA_G Cuts at: 820 1017 1943 3643 4544 4806 820 Size: 197 926 1700 901 262 1553 Fragments arranged by size: 1700 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3330 493 Size: 2837 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5539 SphI G_CATG'C Cuts at: 1132 2897 1132 Size: 1765 3774 SspI AAT'ATT Cuts at: 5 52 3463 5 Size: 47 3411 2081 Fragments arranged by size: 3411 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5539 StyI C'CwwG_G Cuts at: 513 1065 2004 2305 2757 2978 513 Size: 552 939 301 452 221 3074 Fragments arranged by size: 3074 939 552 452 301 221 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3566 3583 3736 3921 4366 325 Size: 1255 1986 17 153 185 445 1498 Fragments arranged by size: 1986 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2434 2548 3785 Size: 274 80 33 51 1443 457 114 1237 Cuts at: 3785 5497 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1237 457 274 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5499 5531 5499 Size: 32 5507 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5539 XbaI T'CTAG_A Cuts at: 2875 2875 Size: 5539 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5539 XmaI C'CCGG_G Cuts at: 1870 2109 2178 2864 1870 Size: 239 69 686 4545 Fragments arranged by size: 4545 686 239 69 XmnI GAAnn'nnTTC Cuts at: 2667 3668 2667 Size: 1001 4538 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3347 276 Size: 53 83 186 936 1813 2468 Fragments arranged by size: 2468 1813 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI