Restriction map for
pFN21AE6364
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AviII AvrII BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
KspI MaeI MamI MluI MslI
MspA1I MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tsp45I
TspRI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 516 TCCGGA T^CCGGA
AflIII 317 ACATGT A^C[GA][CT]GT
ApaI 848 GGGCCC GGGCC^C
Asp700I 456 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 86 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 379 CCCGGG C^[CT]CG[GA]G
BbrPI 919 CACGTG CAC^GTG
BbvI 433 GCAGC GCAGC(8/12)
BclI 176 TGATCA T^GATCA
BglII 976 AGATCT A^GATCT
BsaI 398 GAGACC GGTCTC(1/5)
BsaWI 516 TCCGGA [AT]^CCGG[AT]
BseAI 516 TCCGGA T^CCGGA
BsmI 952 GAATGC GAATGC(1/-1)
BsoBI 379 CCCGGG C^[CT]CG[GA]G
BspDI 367 ATCGAT AT^CGAT
BspEI 516 TCCGGA T^CCGGA
BspLU11I 317 ACATGT A^CATGT
BsrDI 913 GCAATG GCAATG(2/0)
ClaI 367 ATCGAT AT^CGAT
DraI 762 TTTAAA TTT^AAA
DraII 1000 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 1000 GGGACCC [GA]G^G[ACGT]CC[CT]
FauI 644 CCCGC CCCGC(4/6)
FbaI 176 TGATCA T^GATCA
Fnu4HI 433 GCAGC GC^[ACGT]GC
HincII 667 GTCAAC GT[CT]^[GA]AC
HindII 667 GTCAAC GT[CT]^[GA]AC
ItaI 433 GCAGC GC^[ACGT]GC
MaeIII 858 GTTAC ^GT[ACGT]AC
MfeI 438 CAATTG C^AATTG
MroI 516 TCCGGA T^CCGGA
MunI 438 CAATTG C^AATTG
MwoI 918 GCACGTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 317 ACATGT [GA]CATG^[CT]
PflFI 86 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 919 CACGTG CAC^GTG
PmlI 919 CACGTG CAC^GTG
PpuMI 1000 GGGACCC [GA]G^G[AT]CC[CT]
SmaI 379 CCCGGG CCC^GGG
SnaBI 206 TACGTA TAC^GTA
StuI 187 AGGCCT AGG^CCT
TseI 433 GCAGC G^C[AT]GC
Tth111I 86 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 72 CCATTTACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 379 CCCGGG C^CCGGG
XmaI 379 CCCGGG C^CCGGG
XmnI 456 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 793 GAATTT [GA]^AATT[CT]
808 GAATTT
ApoI 793 GAATTT [GA]^AATT[CT]
808 GAATTT
BalI 59 TGGCCA TGG^CCA
611 TGGCCA
BanII 359 GGGCTC G[GA]GC[CT]^C
848 GGGCCC
BmyI 359 GGGCTC G[AGT]GC[ACT]^C
848 GGGCCC
BpmI 555 CTGGAG CTGGAG(16/14)
943 CTGGAG
BsaAI 206 TACGTA [CT]AC^GT[GA]
919 CACGTG
BseRI 653 GAGGAG GAGGAG(10/8)
1015 GAGGAG
Bsp1286I 359 GGGCTC G[AGT]GC[ACT]^C
848 GGGCCC
BstYI 513 AGATCC [GA]^GATC[CT]
976 AGATCT
EaeI 59 TGGCCA [CT]^GGCC[GA]
611 TGGCCA
EarI 14 CTCTTC CTCTTC(1/4)
821 CTCTTC
Ksp632I 14 CTCTTC CTCTTC(1/4)
821 CTCTTC
MflI 513 AGATCC [GA]^GATC[CT]
976 AGATCT
MluNI 59 TGGCCA TGG^CCA
611 TGGCCA
MscI 59 TGGCCA TGG^CCA
611 TGGCCA
SfaNI 217 GATGC GCATC(5/9)
265 GATGC
SfcI 101 CTATAG C^T[GA][CT]AG
873 CTACAG
TfiI 417 GATTC G^A[AT]TC
939 GAATC
XhoII 513 AGATCC [GA]^GATC[CT]
976 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BcnI BsaJI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
FokI HaeIII HapII HinfI HpaII
HphI MaeII MboI MboII MnlI
MseI MspI MvaI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI TaqI Tru9I Tsp509I
TthHB8I