################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I Eco47III EcoNI EcoO109I EcoO65I EcoRV EcoT14I FseI HgaI HindIII HpaI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MunI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 166 CGCG CG^CG AcyI 133 GGCGCC G[GA]^CG[CT]C AlwI 76 GGATC GGATC(4/5) AocI 529 CCTAAGG CC^T[ACGT]AGG AseI 354 ATTAAT AT^TAAT AsnI 354 ATTAAT AT^TAAT Asp700I 378 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 551 CCCGGG C^[CT]CG[GA]G AvaII 170 GGTCC G^G[AT]CC AviII 2 TGCGCA TGC^GCA BanI 133 GGCGCC G^G[CT][GA]CC BbeI 133 GGCGCC GGCGC^C BbsI 505 GAAGAC GAAGAC(2/6) BclI 566 TGATCA T^GATCA BglII 279 AGATCT A^GATCT BpuAI 505 GAAGAC GAAGAC(2/6) BsaHI 133 GGCGCC G[GA]^CG[CT]C BsoBI 551 CCCGGG C^[CT]CG[GA]G BspHI 662 TCATGA T^CATGA BssSI 213 CTCGTG CACGAG(-5/-1) BstUI 166 CGCG CG^CG BstYI 279 AGATCT [GA]^GATC[CT] Bsu36I 529 CCTAAGG CC^T[ACGT]AGG Eco81I 529 CCTAAGG CC^T[ACGT]AGG EcoT22I 380 ATGCAT ATGCA^T FbaI 566 TGATCA T^GATCA FspI 2 TGCGCA TGC^GCA HaeII 133 GGCGCC [GA]GCGC^[CT] Hin1I 133 GGCGCC G[GA]^CG[CT]C HincII 325 GTCAAC GT[CT]^[GA]AC HindII 325 GTCAAC GT[CT]^[GA]AC KasI 133 GGCGCC G^GCGCC MaeIII 361 GTCAC ^GT[ACGT]AC MflI 279 AGATCT [GA]^GATC[CT] MseI 355 TTAA T^TAA MslI 630 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 166 CGCG CG^CG NarI 133 GGCGCC GG^CGCC NsiI 380 ATGCAT ATGCA^T NspI 178 GCATGC [GA]CATG^[CT] PshBI 354 ATTAAT AT^TAAT RcaI 662 TCATGA T^CATGA SapI 426 GCTCTTC GCTCTTC(1/4) SfoI 133 GGCGCC GGC^GCC SmaI 551 CCCGGG CCC^GGG SphI 178 GCATGC GCATG^C TfiI 659 GATTC G^A[AT]TC Tru9I 355 TTAA T^TAA Tsp45I 361 GTCAC ^GT[GC]AC XhoII 279 AGATCT [GA]^GATC[CT] XmaCI 551 CCCGGG C^CCGGG XmaI 551 CCCGGG C^CCGGG XmnI 378 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcsI 391 GAATTC [GA]^AATT[CT] 406 GAATTC ApoI 391 GAATTC [GA]^AATT[CT] 406 GAATTC BanII 64 GGGCTC G[GA]GC[CT]^C 335 GGGCTC BmyI 64 GGGCTC G[AGT]GC[ACT]^C 335 GGGCTC BpmI 67 CTCCAG CTGGAG(16/14) 365 CTCCAG BsiEI 26 CGGCCG CG[GA][CT]^CG 44 CGGCCG BsiYI 151 CCCGGAGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 152 CCGGAGCGGGG BslI 151 CCCGGAGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 152 CCGGAGCGGGG BsmAI 644 GAGAC GTCTC(1/5) 218 GTCTC BsmI 378 GAATGC GAATGC(1/-1) 382 GCATTC Bsp1286I 64 GGGCTC G[AGT]GC[ACT]^C 335 GGGCTC BsrBI 82 GAGCGG CCGCTC(-3/-3) 155 GAGCGG BsrFI 24 GCCGGC [GA]^CCGG[CT] 46 GCCGGC CfoI 3 GCGC GCG^C 134 GCGC Cfr10I 24 GCCGGC [GA]^CCGG[CT] 46 GCCGGC Cfr13I 170 GGTCC G^G[ACGT]CC 248 GGCCC EaeI 26 CGGCCG [CT]^GGCC[GA] 44 CGGCCG EagI 26 CGGCCG C^GGCCG 44 CGGCCG EarI 427 CTCTTC CTCTTC(1/4) 481 GAAGAG EclXI 26 CGGCCG C^GGCCG 44 CGGCCG Eco52I 26 CGGCCG C^GGCCG 44 CGGCCG EcoRI 391 GAATTC G^AATTC 406 GAATTC HhaI 3 GCGC GCG^C 134 GCGC HinP1I 3 GCGC G^CGC 134 GCGC HphI 537 GGTGA GGTGA(8/7) 555 GGTGA Ksp632I 427 CTCTTC CTCTTC(1/4) 481 GAAGAG NaeI 24 GCCGGC GCC^GGC 46 GCCGGC NgoMIV 24 GCCGGC G^CCGGC 46 GCCGGC Sau96I 170 GGTCC G^G[ACGT]CC 248 GGCCC TaqI 605 TCGA T^CGA 657 TCGA TspRI 222 CACTG CA[GC]TG[ACGT][ACGT]^ 411 CAGTG TthHB8I 605 TCGA T^CGA 657 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI BbvI BcnI BsaJI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI ScrFI SfaNI TseI Tsp509I