(Circular) (Six-base) MAPSORT of: pFN21AE5256.seq Check: 547 from: 1 to: 5428 pFN21AE5256 5428 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3238 278 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2771 1937 Size: 834 4594 Acc65I G'GTAC_C Cuts at: 2749 2749 Size: 5428 AclI AA'CG_TT Cuts at: 1563 3555 3928 1563 Size: 1992 373 3063 Fragments arranged by size: 3063 1992 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2609 2681 3491 4875 5143 1632 Size: 977 72 810 1384 268 1917 Fragments arranged by size: 1917 1384 977 810 268 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5231 AflIII A'CryG_T Cuts at: 1203 4327 5304 1203 Size: 3124 977 1327 Fragments arranged by size: 3124 1327 977 AgeI A'CCGG_T Cuts at: 4259 4259 Size: 5428 AhdI GACnn_n'nnGTC Cuts at: 4157 4157 Size: 5428 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5428 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5428 AlwNI CAG_nnn'CTG Cuts at: 3171 4743 3171 Size: 1572 3856 ApaLI G'TGCA_C Cuts at: 3485 4641 3485 Size: 1156 4272 ApoI r'AATT_y Cuts at: 1493 2396 2411 2737 2963 3115 5224 1493 Size: 903 15 326 226 152 2109 1697 Fragments arranged by size: 2109 1697 903 326 226 152 15 AseI AT'TA_AT Cuts at: 160 1032 2360 3982 160 Size: 872 1328 1622 1606 Fragments arranged by size: 1622 1606 1328 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 AvaI C'yCGr_G Cuts at: 1870 1943 2556 2753 1870 Size: 73 613 197 4545 Fragments arranged by size: 4545 613 197 73 BamHI G'GATC_C Cuts at: 2758 3125 2758 Size: 367 5061 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 2749 4204 618 Size: 325 217 627 351 611 1455 1842 Fragments arranged by size: 1842 1455 627 611 351 325 217 BanII G_rGCy'C Cuts at: 1391 2073 2344 2747 1391 Size: 682 271 403 4072 Fragments arranged by size: 4072 682 403 271 BbeI G_GCGC'C Cuts at: 2142 2142 Size: 5428 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2517 928 Size: 971 618 3839 Fragments arranged by size: 3839 971 618 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3619 3653 1903 Size: 34 50 34 1598 34 3678 Fragments arranged by size: 3678 1598 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3320 4530 3320 Size: 1210 4218 BclI T'GATC_A Cuts at: 1548 2571 1548 Size: 1023 4405 BfrBI ATG'CAT Cuts at: 2387 2387 Size: 5428 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4039 136 Size: 107 122 71 631 605 2367 1525 Fragments arranged by size: 2367 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2284 5423 2284 Size: 3139 2289 BlpI GC'TnA_GC Cuts at: 2845 2845 Size: 5428 Bme1580I G_kGCm'C Cuts at: 1790 3489 4645 1790 Size: 1699 1156 2573 Fragments arranged by size: 2573 1699 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5428 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3159 4117 442 Size: 416 53 83 399 24 1742 958 1753 Fragments arranged by size: 1753 1742 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5428 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2355 4088 1239 Size: 818 298 1733 2579 Fragments arranged by size: 2579 1733 818 298 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3553 4418 4716 3553 Size: 865 298 4265 Fragments arranged by size: 4265 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4091 882 Size: 617 2592 2219 Fragments arranged by size: 2592 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3219 493 Size: 711 2015 2702 Fragments arranged by size: 2702 2015 711 BsaBI GATnn'nnATC Cuts at: 5422 5422 Size: 5428 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2139 3235 3617 4252 Size: 53 83 186 936 606 1096 382 635 Cuts at: 4252 275 Size: 1451 Fragments arranged by size: 1451 1096 936 635 606 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3861 4259 4533 4680 5091 3861 Size: 398 274 147 411 4198 Fragments arranged by size: 4198 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5203 Fragments arranged by size: 5203 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5428 BseYI C'CCAG_C Cuts at: 4631 5127 4631 Size: 496 4932 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4855 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 2052 2878 3639 4667 1540 Size: 275 186 33 18 826 761 1028 2301 Fragments arranged by size: 2301 1028 826 761 275 186 33 18 BsiHKAI G_wGCw'C Cuts at: 3489 3574 4645 3489 Size: 85 1071 4272 Fragments arranged by size: 4272 1071 85 BsmI GAATG_Cn' Cuts at: 2387 2389 2939 3032 2387 Size: 2 550 93 4783 Fragments arranged by size: 4783 550 93 2 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2344 2747 3489 3574 4645 1391 Size: 399 283 271 403 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 403 399 283 271 85 BspEI T'CCGG_A Cuts at: 5091 5091 Size: 5428 BspHI T'CATG_A Cuts at: 2667 3317 5047 2667 Size: 650 1730 3048 Fragments arranged by size: 3048 1730 650 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2783 844 Size: 1939 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2089 2162 3315 1394 Size: 429 266 73 1153 3507 Fragments arranged by size: 3507 1153 429 266 73 BsrDI GCAATG_nn' Cuts at: 65 1248 3923 4097 65 Size: 1183 2675 174 1396 Fragments arranged by size: 2675 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2029 2051 4072 4259 1700 Size: 110 219 22 2021 187 2869 Fragments arranged by size: 2869 2021 219 187 110 22 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5428 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5428 BssSI C'ACGA_G Cuts at: 2218 3488 4500 2218 Size: 1270 1012 3146 Fragments arranged by size: 3146 1270 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5428 BstBI TT'CG_AA Cuts at: 4232 4232 Size: 5428 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5428 BstYI r'GATC_y Cuts at: 1972 2284 2758 3125 3510 3527 4979 5065 5077 Size: 312 474 367 385 17 1452 86 12 Cuts at: 5077 5423 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 474 385 367 346 312 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2535 2535 Size: 5428 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4876 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4689 Fragments arranged by size: 4689 683 56 BtsI GCAGTG_nn' Cuts at: 2944 3738 3758 2944 Size: 794 20 4614 Fragments arranged by size: 4614 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5428 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5393 DraI TTT'AAA Cuts at: 2732 2732 Size: 5428 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4435 809 Size: 507 3119 1802 Fragments arranged by size: 3119 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2049 2875 Size: 54 1425 50 275 18 201 18 826 Cuts at: 2875 3764 5393 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 826 275 201 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2031 2049 2875 1537 Size: 275 219 18 826 4090 Fragments arranged by size: 4090 826 275 219 18 EarI CTCTTCn'nnn_ Cuts at: 1353 2438 2481 3358 1353 Size: 1085 43 877 3423 Fragments arranged by size: 3423 1085 877 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2030 4015 4389 4535 387 Size: 1064 138 441 1985 374 146 1280 Fragments arranged by size: 1985 1280 1064 441 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2355 2609 2681 3491 4088 4875 Size: 393 425 298 254 72 810 597 787 Cuts at: 4875 5143 1239 Size: 268 1524 Fragments arranged by size: 1524 810 787 597 425 393 298 268 254 72 EcoRI G'AATT_C Cuts at: 2396 2411 2737 3115 2396 Size: 15 326 378 4709 Fragments arranged by size: 4709 378 326 15 FspI TGC'GCA Cuts at: 2009 3934 2009 Size: 1925 3503 FspAI rTGC'GCAy Cuts at: 2009 2009 Size: 5428 HaeII r_GCGC'y Cuts at: 1678 2142 4575 1678 Size: 464 2433 2531 Fragments arranged by size: 2531 2433 464 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2556 2588 4069 4101 4143 Size: 32 135 32 984 32 1481 32 42 Cuts at: 4143 4175 1373 Size: 32 2626 Fragments arranged by size: 2626 1481 984 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2332 2772 3018 669 Size: 988 281 394 440 246 3079 Fragments arranged by size: 3079 988 440 394 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5428 HpaI GTT'AAC Cuts at: 3018 3018 Size: 5428 KasI G'GCGC_C Cuts at: 2138 2138 Size: 5428 KpnI G_GTAC'C Cuts at: 2753 2753 Size: 5428 MfeI C'AATT_G Cuts at: 3027 3027 Size: 5428 MluI A'CGCG_T Cuts at: 5304 5304 Size: 5428 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2202 2315 4542 4726 1305 Size: 897 113 2227 184 2007 Fragments arranged by size: 2227 2007 897 184 113 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3606 Fragments arranged by size: 3606 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2639 3386 3745 3904 518 Size: 648 1473 747 359 159 2042 Fragments arranged by size: 2042 1473 747 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2844 3521 4669 4914 1606 Size: 1238 677 1148 245 2120 Fragments arranged by size: 2120 1238 1148 677 245 NaeI GCC'GGC Cuts at: 2031 2053 2031 Size: 22 5406 NarI GG'CG_CC Cuts at: 2139 2139 Size: 5428 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4876 NdeI CA'TA_TG Cuts at: 387 387 Size: 5428 NgoMIV G'CCGG_C Cuts at: 2029 2051 2029 Size: 22 5406 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5428 NotI GC'GGCC_GC Cuts at: 2875 2875 Size: 5428 NsiI A_TGCA'T Cuts at: 2389 2389 Size: 5428 NspI r_CATG'y Cuts at: 1132 1229 2187 2786 4331 1132 Size: 97 958 599 1545 2229 Fragments arranged by size: 2229 1545 958 599 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5428 PciI A'CATG_T Cuts at: 4327 4327 Size: 5428 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5428 PmeI GTTT'AAAC Cuts at: 2732 2732 Size: 5428 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3497 3529 5010 5042 3497 Size: 32 1481 32 3883 Fragments arranged by size: 3883 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5428 PsiI TTA'TAA Cuts at: 2998 2998 Size: 5428 PstI C_TGCA'G Cuts at: 830 2780 830 Size: 1950 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5428 SalI G'TCGA_C Cuts at: 1936 2770 1936 Size: 834 4594 SapI GCTCTTCn'nnn_ Cuts at: 2438 2438 Size: 5428 SbfI CC_TGCA'GG Cuts at: 2780 2780 Size: 5428 ScaI AGT'ACT Cuts at: 3676 5388 3676 Size: 1712 3716 SfcI C'TryA_G Cuts at: 826 1045 2776 3911 4592 4783 826 Size: 219 1731 1135 681 191 1471 Fragments arranged by size: 1731 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2140 2140 Size: 5428 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5428 SmaI CCC'GGG Cuts at: 1872 2558 2755 1872 Size: 686 197 4545 Fragments arranged by size: 4545 686 197 SmlI C'TyrA_G Cuts at: 820 1017 1943 3532 4433 4695 820 Size: 197 926 1589 901 262 1553 Fragments arranged by size: 1589 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3219 493 Size: 2726 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5428 SphI G_CATG'C Cuts at: 1132 2187 2786 1132 Size: 1055 599 3774 Fragments arranged by size: 3774 1055 599 SspI AAT'ATT Cuts at: 5 52 3352 5 Size: 47 3300 2081 Fragments arranged by size: 3300 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5428 StyI C'CwwG_G Cuts at: 513 1065 2867 513 Size: 552 1802 3074 Fragments arranged by size: 3074 1802 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3455 3472 3625 3810 4255 325 Size: 1255 1875 17 153 185 445 1498 Fragments arranged by size: 1875 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3674 5386 96 Size: 274 80 33 51 1443 1697 1712 138 Fragments arranged by size: 1712 1697 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2431 2463 5388 5420 2431 Size: 32 2925 32 2439 Fragments arranged by size: 2925 2439 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5428 XbaI T'CTAG_A Cuts at: 2764 2764 Size: 5428 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5428 XmaI C'CCGG_G Cuts at: 1870 2556 2753 1870 Size: 686 197 4545 Fragments arranged by size: 4545 686 197 XmnI GAAnn'nnTTC Cuts at: 2387 3557 2387 Size: 1170 4258 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3236 276 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI