(Linear) (Six-base) MAPSORT of: KIEE5256.seq Check: 194 from: 1 to: 723 KIEE5256.orf With 182 enzymes: SgfI * January 29, 2014 11:53 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 676 723 Size: 604 72 47 Fragments arranged by size: 604 72 47 ApoI r'AATT_y Cuts at: 0 391 406 723 Size: 391 15 317 Fragments arranged by size: 391 317 15 AseI AT'TA_AT Cuts at: 0 355 723 Size: 355 368 AvaI C'yCGr_G Cuts at: 0 551 723 Size: 551 172 BanI G'GyrC_C Cuts at: 0 133 723 Size: 133 590 BanII G_rGCy'C Cuts at: 0 68 339 723 Size: 68 271 384 Fragments arranged by size: 384 271 68 BbeI G_GCGC'C Cuts at: 0 137 723 Size: 137 586 BbsI GAAGACnn'nnnn_ Cuts at: 0 512 723 Size: 512 211 BclI T'GATC_A Cuts at: 0 566 723 Size: 566 157 BfrBI ATG'CAT Cuts at: 0 382 723 Size: 382 341 BglII A'GATC_T Cuts at: 0 279 723 Size: 279 444 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 350 723 Size: 52 298 373 Fragments arranged by size: 373 298 52 BsaHI Gr'CG_yC Cuts at: 0 134 723 Size: 134 589 BsiEI CG_ry'CG Cuts at: 0 29 47 723 Size: 29 18 676 Fragments arranged by size: 676 29 18 BsmI GAATG_Cn' Cuts at: 0 382 384 723 Size: 382 2 339 Fragments arranged by size: 382 339 2 Bsp1286I G_dGCh'C Cuts at: 0 68 339 723 Size: 68 271 384 Fragments arranged by size: 384 271 68 BspHI T'CATG_A Cuts at: 0 662 723 Size: 662 61 BsrBI CCG'CTC Cuts at: 0 84 157 723 Size: 84 73 566 Fragments arranged by size: 566 84 73 BsrFI r'CCGG_y Cuts at: 0 24 46 723 Size: 24 22 677 Fragments arranged by size: 677 24 22 BssSI C'ACGA_G Cuts at: 0 213 723 Size: 213 510 BstYI r'GATC_y Cuts at: 0 279 723 Size: 279 444 Bsu36I CC'TnA_GG Cuts at: 0 530 723 Size: 530 193 EaeI y'GGCC_r Cuts at: 0 26 44 723 Size: 26 18 679 Fragments arranged by size: 679 26 18 EagI C'GGCC_G Cuts at: 0 26 44 723 Size: 26 18 679 Fragments arranged by size: 679 26 18 EarI CTCTTCn'nnn_ Cuts at: 0 433 476 723 Size: 433 43 247 Fragments arranged by size: 433 247 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 25 723 Size: 25 698 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 350 604 676 723 Size: 52 298 254 72 47 Fragments arranged by size: 298 254 72 52 47 EcoRI G'AATT_C Cuts at: 0 391 406 723 Size: 391 15 317 Fragments arranged by size: 391 317 15 FspI TGC'GCA Cuts at: 0 4 723 Size: 4 719 FspAI rTGC'GCAy Cuts at: 0 4 723 Size: 4 719 HaeII r_GCGC'y Cuts at: 0 137 723 Size: 137 586 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 551 583 723 Size: 551 32 140 Fragments arranged by size: 551 140 32 HincII GTy'rAC Cuts at: 0 327 723 Size: 327 396 KasI G'GCGC_C Cuts at: 0 133 723 Size: 133 590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 197 310 723 Size: 197 113 413 Fragments arranged by size: 413 197 113 MslI CAynn'nnrTG Cuts at: 0 634 723 Size: 634 89 NaeI GCC'GGC Cuts at: 0 26 48 723 Size: 26 22 675 Fragments arranged by size: 675 26 22 NarI GG'CG_CC Cuts at: 0 134 723 Size: 134 589 NgoMIV G'CCGG_C Cuts at: 0 24 46 723 Size: 24 22 677 Fragments arranged by size: 677 24 22 NsiI A_TGCA'T Cuts at: 0 384 723 Size: 384 339 NspI r_CATG'y Cuts at: 0 182 723 Size: 182 541 SapI GCTCTTCn'nnn_ Cuts at: 0 433 723 Size: 433 290 SfoI GGC'GCC Cuts at: 0 135 723 Size: 135 588 SmaI CCC'GGG Cuts at: 0 553 723 Size: 553 170 SphI G_CATG'C Cuts at: 0 182 723 Size: 182 541 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 426 458 723 Size: 426 32 265 Fragments arranged by size: 426 265 32 XmaI C'CCGG_G Cuts at: 0 551 723 Size: 551 172 XmnI GAAnn'nnTTC Cuts at: 0 382 723 Size: 382 341 Enzymes that do cut: AcuI ApoI AseI AvaI BanI BanII BbeI BbsI BclI BfrBI BglII BpmI BsaHI BsiEI BsmI Bsp1286I BspHI BsrBI BsrFI BssSI BstYI Bsu36I EaeI EagI EarI EciI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII KasI MmeI MslI NaeI NarI NgoMIV NsiI NspI SapI SfoI SmaI SphI TstI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI BspEI BspMI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoO109I EcoRV FalI FseI HindIII HpaI KpnI MfeI MluI MscI MspA1I NcoI NdeI NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI TaqII TatI Tth111I XbaI XcmI XhoI ZraI