(Circular) (Six-base) MAPSORT of: pFN21AE3103.seq Check: 547 from: 1 to: 5512 pFN21AE3103 5512 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2407 2407 Size: 5512 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3322 278 Size: 53 83 186 936 1786 2468 Fragments arranged by size: 2468 1786 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2855 1937 Size: 918 4594 Acc65I G'GTAC_C Cuts at: 2833 2833 Size: 5512 AclI AA'CG_TT Cuts at: 1563 3639 4012 1563 Size: 2076 373 3063 Fragments arranged by size: 3063 2076 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2005 2170 3575 4959 5227 1632 Size: 373 165 1405 1384 268 1917 Fragments arranged by size: 1917 1405 1384 373 268 165 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5315 AflIII A'CryG_T Cuts at: 1203 4411 5388 1203 Size: 3208 977 1327 Fragments arranged by size: 3208 1327 977 AgeI A'CCGG_T Cuts at: 4343 4343 Size: 5512 AhdI GACnn_n'nnGTC Cuts at: 4241 4241 Size: 5512 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5512 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5512 AlwNI CAG_nnn'CTG Cuts at: 3255 4827 3255 Size: 1572 3940 ApaI G_GGCC'C Cuts at: 2128 2295 2336 2342 2564 2128 Size: 167 41 6 222 5076 Fragments arranged by size: 5076 222 167 41 6 ApaLI G'TGCA_C Cuts at: 3569 4725 3569 Size: 1156 4356 ApoI r'AATT_y Cuts at: 1493 2821 3047 3199 5308 1493 Size: 1328 226 152 2109 1697 Fragments arranged by size: 2109 1697 1328 226 152 AscI GG'CGCG_CC Cuts at: 2046 2046 Size: 5512 AseI AT'TA_AT Cuts at: 160 1032 4066 160 Size: 872 3034 1606 Fragments arranged by size: 3034 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5512 AvaI C'yCGr_G Cuts at: 1870 1943 2335 2535 2579 2837 1870 Size: 73 392 200 44 258 4545 Fragments arranged by size: 4545 392 258 200 73 44 AvrII C'CTAG_G Cuts at: 2128 2128 Size: 5512 BamHI G'GATC_C Cuts at: 2842 3209 2842 Size: 367 5145 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2141 2833 4288 618 Size: 325 217 627 354 692 1455 1842 Fragments arranged by size: 1842 1455 692 627 354 325 217 BanII G_rGCy'C Cuts at: 1391 2128 2247 2295 2336 2342 2373 2564 2831 Size: 737 119 48 41 6 31 191 267 Cuts at: 2831 1391 Size: 4072 Fragments arranged by size: 4072 737 267 191 119 48 41 31 6 BbeI G_GCGC'C Cuts at: 2145 2145 Size: 5512 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4541 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2593 2627 3703 3737 1903 Size: 34 656 34 1076 34 3678 Fragments arranged by size: 3678 1076 656 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3404 4614 3404 Size: 1210 4302 BclI T'GATC_A Cuts at: 1548 1548 Size: 5512 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2031 2094 2331 Size: 107 122 71 631 605 359 63 237 Cuts at: 2331 4123 136 Size: 1792 1525 Fragments arranged by size: 1792 1525 631 605 359 237 122 107 71 63 BglII A'GATC_T Cuts at: 5507 5507 Size: 5512 BlpI GC'TnA_GC Cuts at: 2929 2929 Size: 5512 Bme1580I G_kGCm'C Cuts at: 1790 2128 2220 2295 2336 2342 2564 3573 4729 Size: 338 92 75 41 6 222 1009 1156 Cuts at: 4729 1790 Size: 2573 Fragments arranged by size: 2573 1156 1009 338 222 92 75 41 6 BmgBI CAC'GTC Cuts at: 1318 2481 1318 Size: 1163 4349 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2386 3243 4201 Size: 416 53 83 399 24 969 857 958 Cuts at: 4201 442 Size: 1753 Fragments arranged by size: 1753 969 958 857 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5512 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2246 2318 4172 1239 Size: 1007 72 1854 2579 Fragments arranged by size: 2579 1854 1007 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2345 3637 4502 4800 2345 Size: 1292 865 298 3057 Fragments arranged by size: 3057 1292 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4175 882 Size: 617 2676 2219 Fragments arranged by size: 2676 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3303 493 Size: 711 2099 2702 Fragments arranged by size: 2702 2099 711 BsaBI GATnn'nnATC Cuts at: 5506 5506 Size: 5512 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2142 3319 3701 4336 Size: 53 83 186 936 609 1177 382 635 Cuts at: 4336 275 Size: 1451 Fragments arranged by size: 1451 1177 936 635 609 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3945 4343 4617 4764 5175 3945 Size: 398 274 147 411 4282 Fragments arranged by size: 4282 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2186 2216 1178 Size: 30 165 30 783 30 4474 Fragments arranged by size: 4474 783 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5512 BseYI C'CCAG_C Cuts at: 2768 4715 5211 2768 Size: 1947 496 3069 Fragments arranged by size: 3069 1947 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2687 1163 Size: 573 951 3988 Fragments arranged by size: 3988 951 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2457 2790 2962 3723 4751 1540 Size: 275 186 456 333 172 761 1028 2301 Fragments arranged by size: 2301 1028 761 456 333 275 186 172 BsiHKAI G_wGCw'C Cuts at: 2247 3573 3658 4729 2247 Size: 1326 85 1071 3030 Fragments arranged by size: 3030 1326 1071 85 BsmI GAATG_Cn' Cuts at: 3023 3116 3023 Size: 93 5419 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2128 2220 2247 2295 2336 2342 2373 Size: 399 338 92 27 48 41 6 31 Cuts at: 2373 2564 2831 3573 3658 4729 1391 Size: 191 267 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 338 267 191 92 85 48 41 31 27 6 BspEI T'CCGG_A Cuts at: 5175 5175 Size: 5512 BspHI T'CATG_A Cuts at: 3401 5131 3401 Size: 1730 3782 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2407 2867 844 Size: 1563 460 3489 Fragments arranged by size: 3489 1563 460 BsrBI CCG'CTC Cuts at: 1394 1823 2348 3399 1394 Size: 429 525 1051 3507 Fragments arranged by size: 3507 1051 525 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4007 4181 65 Size: 1183 2759 174 1396 Fragments arranged by size: 2759 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2549 2586 2738 2785 4156 4343 1700 Size: 110 739 37 152 47 1371 187 2869 Fragments arranged by size: 2869 1371 739 187 152 110 47 37 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5512 BssHII G'CGCG_C Cuts at: 1925 2039 2046 2321 1925 Size: 114 7 275 5116 Fragments arranged by size: 5116 275 114 7 BssSI C'ACGA_G Cuts at: 3572 4584 3572 Size: 1012 4500 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5512 BstBI TT'CG_AA Cuts at: 4316 4316 Size: 5512 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5512 BstYI r'GATC_y Cuts at: 1972 2842 3209 3594 3611 5063 5149 5161 5507 Size: 870 367 385 17 1452 86 12 346 Cuts at: 5507 1972 Size: 1977 Fragments arranged by size: 1977 1452 870 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2107 2205 2439 2569 513 Size: 552 1042 98 234 130 3456 Fragments arranged by size: 3456 1042 552 234 130 98 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2596 487 Size: 683 56 1370 3403 Fragments arranged by size: 3403 1370 683 56 BtsI GCAGTG_nn' Cuts at: 3028 3822 3842 3028 Size: 794 20 4698 Fragments arranged by size: 4698 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5512 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5477 DraI TTT'AAA Cuts at: 2816 2816 Size: 5512 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4519 809 Size: 507 3203 1802 Fragments arranged by size: 3203 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2421 2454 Size: 54 1425 50 275 18 378 213 33 Cuts at: 2454 2736 2787 2959 3848 5477 8 Size: 282 51 172 889 1629 43 Fragments arranged by size: 1629 1425 889 378 282 275 213 172 54 51 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2454 2787 2959 1537 Size: 275 642 333 172 4090 Fragments arranged by size: 4090 642 333 275 172 EarI CTCTTCn'nnn_ Cuts at: 1353 2024 2252 2264 2537 3442 1353 Size: 671 228 12 273 905 3423 Fragments arranged by size: 3423 905 671 273 228 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4099 4473 4619 387 Size: 1064 138 2510 374 146 1280 Fragments arranged by size: 2510 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2245 2245 Size: 5512 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2005 2170 2246 2318 3575 4172 4959 Size: 393 373 165 76 72 1257 597 787 Cuts at: 4959 5227 1239 Size: 268 1524 Fragments arranged by size: 1524 1257 787 597 393 373 268 165 76 72 EcoO109I rG'GnC_Cy Cuts at: 2124 2125 2291 2292 2339 2560 2731 2124 Size: 1 166 1 47 221 171 4905 Fragments arranged by size: 4905 221 171 166 47 1 1 EcoRI G'AATT_C Cuts at: 2821 3199 2821 Size: 378 5134 FseI GG_CCGG'CC Cuts at: 2742 2742 Size: 5512 FspI TGC'GCA Cuts at: 2431 2621 4018 2431 Size: 190 1397 3925 Fragments arranged by size: 3925 1397 190 FspAI rTGC'GCAy Cuts at: 2431 2431 Size: 5512 HaeII r_GCGC'y Cuts at: 1678 2013 2145 2178 2193 2550 4659 1678 Size: 335 132 33 15 357 2109 2531 Fragments arranged by size: 2531 2109 357 335 132 33 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4153 4185 4227 4259 1373 Size: 32 135 32 2581 32 42 32 2626 Fragments arranged by size: 2626 2581 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2856 3102 669 Size: 988 281 918 246 3079 Fragments arranged by size: 3079 988 918 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5512 HpaI GTT'AAC Cuts at: 3102 3102 Size: 5512 KasI G'GCGC_C Cuts at: 2141 2141 Size: 5512 KpnI G_GTAC'C Cuts at: 2837 2837 Size: 5512 MfeI C'AATT_G Cuts at: 3111 3111 Size: 5512 MluI A'CGCG_T Cuts at: 5388 5388 Size: 5512 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4626 4810 1305 Size: 3321 184 2007 Fragments arranged by size: 3321 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3690 Fragments arranged by size: 3690 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2615 3470 3829 3988 518 Size: 648 1449 855 359 159 2042 Fragments arranged by size: 2042 1449 855 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2109 2571 2593 2764 2928 3605 4753 4998 Size: 503 462 22 171 164 677 1148 245 Cuts at: 4998 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 503 462 245 171 164 22 NaeI GCC'GGC Cuts at: 2551 2740 2787 2551 Size: 189 47 5276 Fragments arranged by size: 5276 189 47 NarI GG'CG_CC Cuts at: 2142 2142 Size: 5512 NcoI C'CATG_G Cuts at: 513 1065 2205 513 Size: 552 1140 3820 Fragments arranged by size: 3820 1140 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5512 NgoMIV G'CCGG_C Cuts at: 2549 2738 2785 2549 Size: 189 47 5276 Fragments arranged by size: 5276 189 47 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5512 NotI GC'GGCC_GC Cuts at: 2454 2959 2454 Size: 505 5007 NspI r_CATG'y Cuts at: 1132 1229 2870 4415 1132 Size: 97 1641 1545 2229 Fragments arranged by size: 2229 1641 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5512 PciI A'CATG_T Cuts at: 4411 4411 Size: 5512 PflMI CCAn_nnn'nTGG Cuts at: 1108 2713 1108 Size: 1605 3907 PmeI GTTT'AAAC Cuts at: 2816 2816 Size: 5512 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3581 3613 5094 5126 3581 Size: 32 1481 32 3967 Fragments arranged by size: 3967 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2731 2731 Size: 5512 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5512 PsiI TTA'TAA Cuts at: 3082 3082 Size: 5512 PspOMI G'GGCC_C Cuts at: 2124 2291 2332 2338 2560 2124 Size: 167 41 6 222 5076 Fragments arranged by size: 5076 222 167 41 6 PstI C_TGCA'G Cuts at: 830 2864 830 Size: 2034 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5512 PvuII CAG'CTG Cuts at: 2764 2764 Size: 5512 RsrII CG'GwC_CG Cuts at: 2566 2566 Size: 5512 SacI G_AGCT'C Cuts at: 2247 2247 Size: 5512 SacII CC_GC'GG Cuts at: 2110 2572 2110 Size: 462 5050 SalI G'TCGA_C Cuts at: 1936 2854 1936 Size: 918 4594 SapI GCTCTTCn'nnn_ Cuts at: 2024 2252 2537 2024 Size: 228 285 4999 Fragments arranged by size: 4999 285 228 SbfI CC_TGCA'GG Cuts at: 2864 2864 Size: 5512 ScaI AGT'ACT Cuts at: 3760 5472 3760 Size: 1712 3800 SfcI C'TryA_G Cuts at: 826 1045 2860 3995 4676 4867 826 Size: 219 1815 1135 681 191 1471 Fragments arranged by size: 1815 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2143 2143 Size: 5512 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5512 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5512 SmaI CCC'GGG Cuts at: 1872 2337 2537 2839 1872 Size: 465 200 302 4545 Fragments arranged by size: 4545 465 302 200 SmlI C'TyrA_G Cuts at: 820 1017 1943 2360 3616 4517 4779 820 Size: 197 926 417 1256 901 262 1553 Fragments arranged by size: 1553 1256 926 901 417 262 197 SnaBI TAC'GTA Cuts at: 493 3303 493 Size: 2810 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5512 SphI G_CATG'C Cuts at: 1132 2870 1132 Size: 1738 3774 SrfI GCCC'GGGC Cuts at: 2337 2337 Size: 5512 SspI AAT'ATT Cuts at: 5 52 3436 5 Size: 47 3384 2081 Fragments arranged by size: 3384 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5512 StyI C'CwwG_G Cuts at: 513 1065 2128 2205 2328 2951 513 Size: 552 1063 77 123 623 3074 Fragments arranged by size: 3074 1063 623 552 123 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3539 3556 3709 3894 4339 325 Size: 1255 1959 17 153 185 445 1498 Fragments arranged by size: 1959 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3758 5470 96 Size: 274 80 33 51 1443 1781 1712 138 Fragments arranged by size: 1781 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5472 5504 5472 Size: 32 5480 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5512 XbaI T'CTAG_A Cuts at: 2848 2848 Size: 5512 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5512 XmaI C'CCGG_G Cuts at: 1870 2335 2535 2837 1870 Size: 465 200 302 4545 Fragments arranged by size: 4545 465 302 200 XmnI GAAnn'nnTTC Cuts at: 3641 3641 Size: 5512 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3320 276 Size: 53 83 186 936 1786 2468 Fragments arranged by size: 2468 1786 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI NruI NsiI PacI PfoI PmlI PspXI PsrI SanDI SexAI SfiI SwaI XcmI