################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsoBI Bsp1286I BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT14I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI StuI StyI SwaI Tsp45I Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 386 GTAGAC GT^[AC][GT]AC AcsI 677 GAATTC [GA]^AATT[CT] AflIII 251 ACATGT A^C[GA][CT]GT AlwI 525 GGATC GGATC(4/5) ApoI 677 GAATTC [GA]^AATT[CT] AseI 466 ATTAAT AT^TAAT AsnI 466 ATTAAT AT^TAAT Asp700I 193 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 2 TGGCCA TGG^CCA BanI 322 GGTGCC G^G[CT][GA]CC BbvI 223 GCTGC GCAGC(8/12) BclI 429 TGATCA T^GATCA BcnI 288 CCCGG CC^[GC]GG BglII 502 AGATCT A^GATCT BsaI 295 GAGACC GGTCTC(1/5) BsaJI 116 CCCTGG C^C[ACGT][ACGT]GG BsaWI 479 ACCGGA [AT]^CCGG[AT] BsgI 271 CTGCAC GTGCAG(16/14) BsmFI 260 GTCCC GGGAC(10/14) BsmI 550 GCATTC GAATGC(1/-1) Bsp1407I 141 TGTACA T^GTACA BspLU11I 251 ACATGT A^CATGT BspMI 219 ACCTGC ACCTGC(4/8) BsrGI 141 TGTACA T^GTACA BstAPI 217 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 396 GGTAACC G^GT[ACGT]ACC BstPI 396 GGTAACC G^GT[ACGT]ACC Cfr13I 576 GGGCC G^G[ACGT]CC DraII 575 GGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 2 TGGCCA [CT]^GGCC[GA] EcoO109I 575 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 396 GGTAACC G^GT[ACGT]ACC EcoRI 677 GAATTC G^AATTC EcoT22I 548 ATGCAT ATGCA^T FbaI 429 TGATCA T^GATCA Fnu4HI 223 GCTGC GC^[ACGT]GC ItaI 223 GCTGC GC^[ACGT]GC MaeIII 397 GTAAC ^GT[ACGT]AC MboII 424 GAAGA GAAGA(8/7) MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MslI 246 CACTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 288 CCCGG CC^[GC]GG NsiI 548 ATGCAT ATGCA^T PshBI 466 ATTAAT AT^TAAT Sau96I 576 GGGCC G^G[ACGT]CC ScaI 75 AGTACT AGT^ACT SfcI 384 CTGTAG C^T[GA][CT]AG SphI 546 GCATGC GCATG^C SspBI 141 TGTACA T^GTACA TseI 223 GCTGC G^C[AT]GC TspRI 246 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 500 CCAGATCTATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 193 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BsmAI 295 GAGAC GTCTC(1/5) 435 GTCTC BsrI 36 ACTGG ACTGG(1/-1) 341 ACTGG BstNI 117 CCTGG CC^[AT]GG 168 CCAGG BstYI 502 AGATCT [GA]^GATC[CT] 525 GGATCT EcoRII 117 CCTGG ^CC[AT]GG 168 CCAGG HaeIII 3 GGCC GG^CC 577 GGCC HapII 289 CCGG C^CGG 480 CCGG HinfI 46 GATTC G^A[ACGT]TC 485 GATTC HpaII 289 CCGG C^CGG 480 CCGG MflI 502 AGATCT [GA]^GATC[CT] 525 GGATCT MseI 467 TTAA T^TAA 616 TTAA MspI 289 CCGG C^CGG 480 CCGG MvaI 117 CCTGG CC^[AT]GG 168 CCAGG MwoI 103 GCTTTGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 217 GCACCTGCTGC NlaIV 322 GGTGCC GG[ACGT]^[ACGT]CC 575 GGGGCC NspI 251 ACATGT [GA]CATG^[CT] 546 GCATGC TfiI 46 GATTC G^A[AT]TC 485 GATTC Tru9I 467 TTAA T^TAA 616 TTAA Tsp509I 519 AATT ^AATT 678 AATT XbaI 648 TCTAGA T^CTAGA 690 TCTAGA XhoII 502 AGATCT [GA]^GATC[CT] 525 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AfaI AluI BfaI BssKI BstF5I Cac8I DdeI DpnII FokI HphI MaeI MboI MnlI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI TaqI TthHB8I