################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AvaI AviII AvrII BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII HapII Hin1I HincII HindII HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MluI MroI MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 325 CGCG CG^CG AciI 987 CCGC CCGC(-3/-1) AclI 160 AACGTT AA^CGTT AseI 733 ATTAAT AT^TAAT AsnI 733 ATTAAT AT^TAAT AspHI 1270 GAGCAC G[AT]GC[AT]^C AspI 169 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 291 GGATCC G^GATCC BbsI 1023 GAAGAC GAAGAC(2/6) BpuAI 1023 GAAGAC GAAGAC(2/6) BsaAI 1372 TACGTG [CT]AC^GT[GA] BseRI 100 GAGGAG GAGGAG(10/8) BsiHKAI 1270 GAGCAC G[AT]GC[AT]^C BsmFI 898 GTCCC GGGAC(10/14) BsmI 1180 GAATGC GAATGC(1/-1) BspMI 917 ACCTGC ACCTGC(4/8) BstAPI 1002 GCACACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 325 CGCG CG^CG BstYI 291 GGATCC [GA]^GATC[CT] CfoI 285 GCGC GCG^C DdeI 98 CTGAG C^T[ACGT]AG DraI 73 TTTAAA TTT^AAA DraII 1052 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoNI 1132 CCTGCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1052 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRV 481 GATATC GAT^ATC HhaI 285 GCGC GCG^C HinP1I 285 GCGC G^CGC HindIII 1144 AAGCTT A^AGCTT MflI 291 GGATCC [GA]^GATC[CT] MspA1I 205 CAGCTG C[AC]G^C[GT]G MvnI 325 CGCG CG^CG PflFI 169 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1052 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 733 ATTAAT AT^TAAT Psp1406I 160 AACGTT AA^CGTT PvuII 205 CAGCTG CAG^CTG TfiI 23 GATTC G^A[AT]TC Tth111I 169 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 291 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 523 AAATTT [GA]^AATT[CT] 1263 AAATTC AfaI 181 GTAC GT^AC 915 GTAC AlwNI 1051 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG 1094 CAGGCTCTG ApoI 523 AAATTT [GA]^AATT[CT] 1263 AAATTC AvaII 897 GGTCC G^G[AT]CC 1053 GGTCC BclI 501 TGATCA T^GATCA 740 TGATCA Cfr13I 897 GGTCC G^G[ACGT]CC 1053 GGTCC DsaI 1155 CCATGG C^C[GA][CT]GG 1379 CCATGG FbaI 501 TGATCA T^GATCA 740 TGATCA HgaI 816 GACGC GACGC(5/10) 258 GCGTC MwoI 80 GCTGTGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1002 GCACACAATGC NcoI 1155 CCATGG C^CATGG 1379 CCATGG PleI 318 GACTC GAGTC(4/5) 444 GACTC PstI 332 CTGCAG CTGCA^G 1436 CTGCAG RsaI 181 GTAC GT^AC 915 GTAC Sau96I 897 GGTCC G^G[ACGT]CC 1053 GGTCC SfaNI 628 GATGC GCATC(5/9) 1102 GATGC XcmI 197 CCATCACCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 884 CCACATCTCTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AluI AlwI BalI BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I DpnII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MvaI NdeII NlaIII NlaIV PflMI SapI Sau3AI ScrFI SfcI StuI StyI Tru9I TseI Tsp45I Tsp509I TspRI Van91I