(Circular) (Six-base) MAPSORT of: pFN21AE4475.seq Check: 7510 from: 1 to: 6187 pFN21AE4475 6187 bp With 182 enzymes: SgfI * March 28, 2012 14:04 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3997 278 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3530 1937 Size: 1593 4594 Acc65I G'GTAC_C Cuts at: 3508 3508 Size: 6187 AclI AA'CG_TT Cuts at: 1563 2166 4314 4687 1563 Size: 603 2148 373 3063 Fragments arranged by size: 3063 2148 603 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3062 3306 4250 5634 5902 1632 Size: 1430 244 944 1384 268 1917 Fragments arranged by size: 1917 1430 1384 944 268 244 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5990 AflIII A'CryG_T Cuts at: 1203 5086 6063 1203 Size: 3883 977 1327 Fragments arranged by size: 3883 1327 977 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 4916 4916 Size: 6187 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6187 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6187 AlwNI CAG_nnn'CTG Cuts at: 3061 3104 3930 5502 3061 Size: 43 826 1572 3746 Fragments arranged by size: 3746 1572 826 43 ApaLI G'TGCA_C Cuts at: 4244 5400 4244 Size: 1156 5031 ApoI r'AATT_y Cuts at: 1493 2528 3268 3496 3722 3874 5983 1493 Size: 1035 740 228 226 152 2109 1697 Fragments arranged by size: 2109 1697 1035 740 228 226 152 AseI AT'TA_AT Cuts at: 160 1032 2739 4741 160 Size: 872 1707 2002 1606 Fragments arranged by size: 2002 1707 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 3512 1870 Size: 73 1569 4545 Fragments arranged by size: 4545 1569 73 BamHI G'GATC_C Cuts at: 2296 3517 3884 2296 Size: 1221 367 4599 Fragments arranged by size: 4599 1221 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3508 4963 618 Size: 325 217 627 1721 1455 1842 Fragments arranged by size: 1842 1721 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3506 1391 Size: 2115 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3035 928 Size: 971 1136 4080 Fragments arranged by size: 4080 1136 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4378 4412 1903 Size: 34 50 34 2357 34 3678 Fragments arranged by size: 3678 2357 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4079 5289 4079 Size: 1210 4977 BclI T'GATC_A Cuts at: 1548 2506 2745 1548 Size: 958 239 4990 Fragments arranged by size: 4990 958 239 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4798 136 Size: 107 122 71 631 605 3126 1525 Fragments arranged by size: 3126 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6182 6182 Size: 6187 BlpI GC'TnA_GC Cuts at: 3604 3604 Size: 6187 Bme1580I G_kGCm'C Cuts at: 1790 3098 3117 4248 5404 1790 Size: 1308 19 1131 1156 2573 Fragments arranged by size: 2573 1308 1156 1131 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6187 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3918 4876 442 Size: 416 53 83 399 24 2501 958 1753 Fragments arranged by size: 2501 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6187 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3093 3093 Size: 6187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4847 1239 Size: 3608 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4312 5177 5475 4312 Size: 865 298 5024 Fragments arranged by size: 5024 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4850 882 Size: 617 3351 2219 Fragments arranged by size: 3351 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3379 3978 493 Size: 711 2175 599 2702 Fragments arranged by size: 2702 2175 711 599 BsaBI GATnn'nnATC Cuts at: 6181 6181 Size: 6187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3994 4376 5011 275 Size: 53 83 186 936 2461 382 635 1451 Fragments arranged by size: 2461 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4620 5018 5292 5439 5850 4620 Size: 398 274 147 411 4957 Fragments arranged by size: 4957 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2434 2464 1178 Size: 30 165 30 1031 30 4901 Fragments arranged by size: 4901 1031 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 1186 Size: 934 5253 BseYI C'CCAG_C Cuts at: 2208 2292 5390 5886 2208 Size: 84 3098 496 2509 Fragments arranged by size: 3098 2509 496 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5614 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3637 4398 5426 1540 Size: 275 186 1636 761 1028 2301 Fragments arranged by size: 2301 1636 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3279 4248 4333 5404 3279 Size: 969 85 1071 4062 Fragments arranged by size: 4062 1071 969 85 BsmI GAATG_Cn' Cuts at: 3191 3698 3791 3191 Size: 507 93 5587 Fragments arranged by size: 5587 507 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3098 3117 3279 3506 4248 4333 5404 Size: 399 1308 19 162 227 742 85 1071 Cuts at: 5404 1391 Size: 2174 Fragments arranged by size: 2174 1308 1071 742 399 227 162 85 19 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 4076 5806 4076 Size: 1730 4457 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2931 3542 844 Size: 2087 611 3489 Fragments arranged by size: 3489 2087 611 BsrBI CCG'CTC Cuts at: 1394 1823 4074 1394 Size: 429 2251 3507 Fragments arranged by size: 3507 2251 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4682 4856 65 Size: 1183 3434 174 1396 Fragments arranged by size: 3434 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4831 5018 1700 Size: 110 3021 187 2869 Fragments arranged by size: 3021 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6187 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6187 BssSI C'ACGA_G Cuts at: 4247 5259 4247 Size: 1012 5175 BstAPI GCAn_nnn'nTGC Cuts at: 794 3013 794 Size: 2219 3968 BstBI TT'CG_AA Cuts at: 4991 4991 Size: 6187 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6187 BstXI CCAn_nnnn'nTGG Cuts at: 2896 3255 3391 2896 Size: 359 136 5692 Fragments arranged by size: 5692 359 136 BstYI r'GATC_y Cuts at: 1972 2296 3517 3884 4269 4286 5738 5824 5836 Size: 324 1221 367 385 17 1452 86 12 Cuts at: 5836 6182 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1221 385 367 346 324 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3160 3384 513 Size: 552 2095 224 3316 Fragments arranged by size: 3316 2095 552 224 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5448 Fragments arranged by size: 5448 683 56 BtsI GCAGTG_nn' Cuts at: 3117 3703 4497 4517 3117 Size: 586 794 20 4787 Fragments arranged by size: 4787 794 586 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6152 DraI TTT'AAA Cuts at: 2080 3491 2080 Size: 1411 4776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5194 809 Size: 507 3878 1802 Fragments arranged by size: 3878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2214 3381 Size: 54 1425 50 275 18 369 15 1167 Cuts at: 3381 3634 4523 6152 8 Size: 253 889 1629 43 Fragments arranged by size: 1629 1425 1167 889 369 275 253 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3634 1537 Size: 275 1822 4090 Fragments arranged by size: 4090 1822 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2120 2331 2424 2670 3329 4117 1353 Size: 767 211 93 246 659 788 3423 Fragments arranged by size: 3423 788 767 659 246 211 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4774 5148 5294 387 Size: 1064 138 3185 374 146 1280 Fragments arranged by size: 3185 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3062 3306 4250 4847 5634 5902 1239 Size: 393 1430 244 944 597 787 268 1524 Fragments arranged by size: 1524 1430 944 787 597 393 268 244 EcoNI CCTnn'n_nnAGG Cuts at: 3141 3141 Size: 6187 EcoO109I rG'GnC_Cy Cuts at: 3058 3058 Size: 6187 EcoRI G'AATT_C Cuts at: 3496 3874 3496 Size: 378 5809 EcoRV GAT'ATC Cuts at: 2488 2488 Size: 6187 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2438 2840 3167 2438 Size: 402 327 5458 Fragments arranged by size: 5458 402 327 FspI TGC'GCA Cuts at: 4693 4693 Size: 6187 HaeII r_GCGC'y Cuts at: 1678 5334 1678 Size: 3656 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4828 4860 4902 4934 1373 Size: 32 135 32 3256 32 42 32 2626 Fragments arranged by size: 3256 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3531 3777 669 Size: 988 281 1593 246 3079 Fragments arranged by size: 3079 1593 988 281 246 HindIII A'AGCT_T Cuts at: 748 3149 748 Size: 2401 3786 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KpnI G_GTAC'C Cuts at: 3512 3512 Size: 6187 MfeI C'AATT_G Cuts at: 3786 3786 Size: 6187 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2196 5301 5485 1305 Size: 891 3105 184 2007 Fragments arranged by size: 3105 2007 891 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2201 2216 3383 10 Size: 54 1425 343 369 15 1167 2814 Fragments arranged by size: 2814 1425 1167 369 343 54 15 MslI CAynn'nnrTG Cuts at: 518 1166 2960 3253 3389 4145 4504 4663 518 Size: 648 1794 293 136 756 359 159 2042 Fragments arranged by size: 2042 1794 756 648 359 293 159 136 MspA1I CmG'CkG Cuts at: 1606 2212 3603 4280 5428 5673 1606 Size: 606 1391 677 1148 245 2120 Fragments arranged by size: 2120 1391 1148 677 606 245 NcoI C'CATG_G Cuts at: 513 1065 3160 3384 513 Size: 552 2095 224 3316 Fragments arranged by size: 3316 2095 552 224 NdeI CA'TA_TG Cuts at: 387 387 Size: 6187 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6187 NotI GC'GGCC_GC Cuts at: 3634 3634 Size: 6187 NspI r_CATG'y Cuts at: 1132 1229 3545 5090 1132 Size: 97 2316 1545 2229 Fragments arranged by size: 2316 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6187 PciI A'CATG_T Cuts at: 5086 5086 Size: 6187 PflMI CCAn_nnn'nTGG Cuts at: 1108 2242 2256 3467 1108 Size: 1134 14 1211 3828 Fragments arranged by size: 3828 1211 1134 14 PfoI T'CCnGG_A Cuts at: 2537 2537 Size: 6187 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4256 4288 5769 5801 4256 Size: 32 1481 32 4642 Fragments arranged by size: 4642 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3058 3058 Size: 6187 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6187 PsiI TTA'TAA Cuts at: 2283 3419 3757 2283 Size: 1136 338 4713 Fragments arranged by size: 4713 1136 338 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2912 2944 2972 3004 2912 Size: 32 28 32 6095 Fragments arranged by size: 6095 32 32 28 PstI C_TGCA'G Cuts at: 830 2341 3445 3539 830 Size: 1511 1104 94 3478 Fragments arranged by size: 3478 1511 1104 94 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 PvuII CAG'CTG Cuts at: 2212 2212 Size: 6187 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SapI GCTCTTCn'nnn_ Cuts at: 2120 2424 3329 2120 Size: 304 905 4978 Fragments arranged by size: 4978 905 304 SbfI CC_TGCA'GG Cuts at: 3539 3539 Size: 6187 ScaI AGT'ACT Cuts at: 4435 6147 4435 Size: 1712 4475 SfcI C'TryA_G Cuts at: 826 1045 2337 3441 3455 3535 4670 5351 5542 Size: 219 1292 1104 14 80 1135 681 191 Cuts at: 5542 826 Size: 1471 Fragments arranged by size: 1471 1292 1135 1104 681 219 191 80 14 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6187 SmaI CCC'GGG Cuts at: 1872 3514 1872 Size: 1642 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4291 5192 5454 820 Size: 197 926 2348 901 262 1553 Fragments arranged by size: 2348 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3978 493 Size: 3485 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6187 SphI G_CATG'C Cuts at: 1132 3545 1132 Size: 2413 3774 SspI AAT'ATT Cuts at: 5 52 4111 5 Size: 47 4059 2081 Fragments arranged by size: 4059 2081 47 StuI AGG'CCT Cuts at: 1517 2155 3123 3136 1517 Size: 638 968 13 4568 Fragments arranged by size: 4568 968 638 13 StyI C'CwwG_G Cuts at: 513 1065 3160 3351 3384 3626 513 Size: 552 2095 191 33 242 3074 Fragments arranged by size: 3074 2095 552 242 191 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2222 2903 3013 3247 4214 4231 4384 Size: 1255 642 681 110 234 967 17 153 Cuts at: 4384 4569 5014 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 967 681 642 445 234 185 153 110 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4433 6145 96 Size: 274 80 33 51 1443 2456 1712 138 Fragments arranged by size: 2456 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2571 2603 6147 6179 2571 Size: 32 3544 32 2579 Fragments arranged by size: 3544 2579 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2177 1316 Size: 861 5326 XbaI T'CTAG_A Cuts at: 3523 3523 Size: 6187 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2209 2896 2209 Size: 687 5500 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6187 XmaI C'CCGG_G Cuts at: 1870 3512 1870 Size: 1642 4545 XmnI GAAnn'nnTTC Cuts at: 4316 4316 Size: 6187 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3995 276 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI