Restriction map for
pFN21AE4475
(Linear) (Six-base) MAPSORT of: KIEE4475.seq Check: 7960 from: 1 to: 1482
KIEE4475.orf
With 182 enzymes: SgfI *
March 22, 2012 18:09 ..
AclI AA'CG_TT
Cuts at: 0 161 1482
Size: 161 1321
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1301 1482
Size: 1057 244 181
Fragments arranged by size:
1057 244 181
AlwNI CAG_nnn'CTG
Cuts at: 0 1056 1099 1482
Size: 1056 43 383
Fragments arranged by size:
1056 383 43
ApoI r'AATT_y
Cuts at: 0 523 1263 1482
Size: 523 740 219
Fragments arranged by size:
740 523 219
AseI AT'TA_AT
Cuts at: 0 734 1482
Size: 734 748
BamHI G'GATC_C
Cuts at: 0 291 1482
Size: 291 1191
BbsI GAAGACnn'nnnn_
Cuts at: 0 1030 1482
Size: 1030 452
BclI T'GATC_A
Cuts at: 0 501 740 1482
Size: 501 239 742
Fragments arranged by size:
742 501 239
Bme1580I G_kGCm'C
Cuts at: 0 1093 1112 1482
Size: 1093 19 370
Fragments arranged by size:
1093 370 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1088 1482
Size: 1088 394
BsaAI yAC'GTr
Cuts at: 0 1374 1482
Size: 1374 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 429 459 1482
Size: 429 30 1023
Fragments arranged by size:
1023 429 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 1482
Size: 115 1367
BseYI C'CCAG_C
Cuts at: 0 203 287 1482
Size: 203 84 1195
Fragments arranged by size:
1195 203 84
BsiHKAI G_wGCw'C
Cuts at: 0 1274 1482
Size: 1274 208
BsmI GAATG_Cn'
Cuts at: 0 1186 1482
Size: 1186 296
Bsp1286I G_dGCh'C
Cuts at: 0 1093 1112 1274 1482
Size: 1093 19 162 208
Fragments arranged by size:
1093 208 162 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 926 1482
Size: 926 556
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1008 1482
Size: 1008 474
BstXI CCAn_nnnn'nTGG
Cuts at: 0 891 1250 1386 1482
Size: 891 359 136 96
Fragments arranged by size:
891 359 136 96
BstYI r'GATC_y
Cuts at: 0 291 1482
Size: 291 1191
BtgI C'CryG_G
Cuts at: 0 1155 1379 1482
Size: 1155 224 103
Fragments arranged by size:
1155 224 103
BtsI GCAGTG_nn'
Cuts at: 0 1112 1482
Size: 1112 370
DraI TTT'AAA
Cuts at: 0 75 1482
Size: 75 1407
EaeI y'GGCC_r
Cuts at: 0 194 209 1376 1482
Size: 194 15 1167 106
Fragments arranged by size:
1167 194 106 15
EarI CTCTTCn'nnn_
Cuts at: 0 115 326 419 665 1324 1482
Size: 115 211 93 246 659 158
Fragments arranged by size:
659 246 211 158 115 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1301 1482
Size: 1057 244 181
Fragments arranged by size:
1057 244 181
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1136 1482
Size: 1136 346
EcoO109I rG'GnC_Cy
Cuts at: 0 1053 1482
Size: 1053 429
EcoRV GAT'ATC
Cuts at: 0 483 1482
Size: 483 999
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 433 835 1162 1482
Size: 433 402 327 320
Fragments arranged by size:
433 402 327 320
HindIII A'AGCT_T
Cuts at: 0 1144 1482
Size: 1144 338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1482
Size: 191 1291
MscI TGG'CCA
Cuts at: 0 196 211 1378 1482
Size: 196 15 1167 104
Fragments arranged by size:
1167 196 104 15
MslI CAynn'nnrTG
Cuts at: 0 955 1248 1384 1482
Size: 955 293 136 98
Fragments arranged by size:
955 293 136 98
MspA1I CmG'CkG
Cuts at: 0 207 1482
Size: 207 1275
NcoI C'CATG_G
Cuts at: 0 1155 1379 1482
Size: 1155 224 103
Fragments arranged by size:
1155 224 103
PflMI CCAn_nnn'nTGG
Cuts at: 0 237 251 1462 1482
Size: 237 14 1211 20
Fragments arranged by size:
1211 237 20 14
PfoI T'CCnGG_A
Cuts at: 0 532 1482
Size: 532 950
PpuMI rG'GwC_Cy
Cuts at: 0 1053 1482
Size: 1053 429
PsiI TTA'TAA
Cuts at: 0 278 1414 1482
Size: 278 1136 68
Fragments arranged by size:
1136 278 68
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 907 939 967 999 1482
Size: 907 32 28 32 483
Fragments arranged by size:
907 483 32 32 28
PstI C_TGCA'G
Cuts at: 0 336 1440 1482
Size: 336 1104 42
Fragments arranged by size:
1104 336 42
PvuII CAG'CTG
Cuts at: 0 207 1482
Size: 207 1275
SapI GCTCTTCn'nnn_
Cuts at: 0 115 419 1324 1482
Size: 115 304 905 158
Fragments arranged by size:
905 304 158 115
SfcI C'TryA_G
Cuts at: 0 332 1436 1450 1482
Size: 332 1104 14 32
Fragments arranged by size:
1104 332 32 14
StuI AGG'CCT
Cuts at: 0 150 1118 1131 1482
Size: 150 968 13 351
Fragments arranged by size:
968 351 150 13
StyI C'CwwG_G
Cuts at: 0 1155 1346 1379 1482
Size: 1155 191 33 103
Fragments arranged by size:
1155 191 103 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 217 898 1008 1242 1482
Size: 217 681 110 234 240
Fragments arranged by size:
681 240 234 217 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 566 598 1482
Size: 566 32 884
Fragments arranged by size:
884 566 32
Tth111I GACn'n_nGTC
Cuts at: 0 172 1482
Size: 172 1310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 204 891 1482
Size: 204 687 591
Fragments arranged by size:
687 591 204
Enzymes that do cut:
AclI AcuI AlwNI ApoI AseI BamHI BbsI BclI
Bme1580I BplI BsaAI BsaXI BseRI BseYI BsiHKAI BsmI
Bsp1286I BspMI BstAPI BstXI BstYI BtgI BtsI DraI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRV FalI HindIII
MmeI MscI MslI MspA1I NcoI PflMI PfoI PpuMI
PsiI PsrI PstI PvuII SapI SfcI StuI StyI
TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BanI BanII BbeI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoRI
FseI FspI FspAI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PshAI PspOMI PspXI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TatI XbaI XhoI XmaI XmnI ZraI