################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## Acc65I AccII AccIII AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AvaI AviII AvrII BalI BanII BbeI BbrPI BciVI BcnI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaI BsaWI BseAI BsiHKAI BsiWI BsoBI Bsp1286I Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HapII HhaI HinP1I HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI PvuI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TspRI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 466 GACGTC GACGT^C AccI 568 GTCGAC GT^[AC][GT]AC AclI 955 AACGTT AA^CGTT AcsI 1258 AAATTT [GA]^AATT[CT] AflIII 1212 ACATGT A^C[GA][CT]GT ApoI 1258 AAATTT [GA]^AATT[CT] Asp700I 1153 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 921 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 532 GGCACC G^G[CT][GA]CC BbsI 864 GTCTTC GAAGAC(2/6) BbvCI 47 CCTCAGC CCTCAGC(-5/-2) BbvI 1183 GCTGC GCAGC(8/12) BclI 554 TGATCA T^GATCA BfaI 1162 CTAG C^TAG BglII 967 AGATCT A^GATCT BpuAI 864 GTCTTC GAAGAC(2/6) BsgI 456 CTGCAC GTGCAG(16/14) BsmAI 687 GAGAC GTCTC(1/5) BsmBI 687 GAGACG CGTCTC(1/5) BsmFI 641 GGGAC GGGAC(10/14) BsmI 612 GAATGC GAATGC(1/-1) BspHI 908 TCATGA T^CATGA BspLU11I 1212 ACATGT A^CATGT BspMI 896 ACCTGC ACCTGC(4/8) BstBI 373 TTCGAA TT^CGAA EaeI 366 CGGCCG [CT]^GGCC[GA] EagI 366 CGGCCG C^GGCCG EclXI 366 CGGCCG C^GGCCG Eco52I 366 CGGCCG C^GGCCG FauI 436 CCCGC CCCGC(4/6) FbaI 554 TGATCA T^GATCA HgaI 689 GACGC GACGC(5/10) HphI 960 TCACC GGTGA(8/7) MaeI 1162 CTAG C^TAG MslI 145 CATTCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 217 CAGCTG C[AC]G^C[GT]G NspI 1212 ACATGT [GA]CATG^[CT] NspV 373 TTCGAA TT^CGAA PflFI 921 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1089 GAGTC GAGTC(4/5) PshAI 390 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 955 AACGTT AA^CGTT PstI 1018 CTGCAG CTGCA^G PvuII 217 CAGCTG CAG^CTG RcaI 908 TCATGA T^CATGA SalI 568 GTCGAC G^TCGAC SfcI 1018 CTGCAG C^T[GA][CT]AG SfuI 373 TTCGAA TT^CGAA SmlI 1268 CTCAAG C^T[CT][GA]AG TseI 1183 GCTGC G^C[AT]GC Tth111I 921 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 803 CCACATCGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1153 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 466 GACGTC G[GA]^CG[CT]C 689 GACGCC AvaII 129 GGTCC G^G[AT]CC 428 GGACC BamHI 296 GGATCC G^GATCC 514 GGATCC BcgI 490 CGACAGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1109 GCAAAGTCCTCG BsaAI 669 CACGTA [CT]AC^GT[GA] 978 CACGTA BsaHI 466 GACGTC G[GA]^CG[CT]C 689 GACGCC BseRI 94 CTCCTC GAGGAG(10/8) 1084 GAGGAG BsiEI 366 CGGCCG CG[GA][CT]^CG 486 CGACCG BsrI 1253 ACTGG ACTGG(1/-1) 1096 CCAGT Cac8I 439 GCCAGC GC[ACGT]^[ACGT]GC 692 GCCTGC Fnu4HI 368 GCCGC GC^[ACGT]GC 1183 GCTGC HaeIII 367 GGCC GG^CC 993 GGCC Hin1I 466 GACGTC G[GA]^CG[CT]C 689 GACGCC ItaI 368 GCCGC GC^[ACGT]GC 1183 GCTGC PflMI 230 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 984 CCACCCCATGG SfaNI 88 GATGC GCATC(5/9) 38 GCATC Tsp45I 469 GTCAC ^GT[GC]AC 919 GTGAC Tsp509I 1034 AATT ^AATT 1259 AATT Van91I 230 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 984 CCACCCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AfaI AluI AlwI BsaJI BsiYI BslI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DsaI EcoRII EcoT14I FokI HincII HindII HinfI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NcoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI StyI TaqI TfiI Tru9I TthHB8I XhoII