(Circular) (Six-base) MAPSORT of: pFN21AE4441.seq Check: 6043 from: 1 to: 6079 pFN21AE4441 6079 bp With 182 enzymes: SgfI * March 28, 2012 14:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2475 3889 278 Size: 53 83 186 936 939 1414 2468 Fragments arranged by size: 2468 1414 939 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2574 3422 1937 Size: 637 848 4594 Fragments arranged by size: 4594 848 637 Acc65I G'GTAC_C Cuts at: 3400 3400 Size: 6079 AclI AA'CG_TT Cuts at: 1563 2961 4206 4579 1563 Size: 1398 1245 373 3063 Fragments arranged by size: 3063 1398 1245 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4142 5526 5794 1632 Size: 2510 1384 268 1917 Fragments arranged by size: 2510 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 3217 4978 5955 1203 Size: 2014 1761 977 1327 Fragments arranged by size: 2014 1761 1327 977 AgeI A'CCGG_T Cuts at: 4910 4910 Size: 6079 AhdI GACnn_n'nnGTC Cuts at: 4808 4808 Size: 6079 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6079 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6079 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3147 3179 3147 Size: 32 6047 AlwNI CAG_nnn'CTG Cuts at: 3822 5394 3822 Size: 1572 4507 ApaLI G'TGCA_C Cuts at: 4136 5292 4136 Size: 1156 4923 ApoI r'AATT_y Cuts at: 1493 3263 3388 3614 3766 5875 1493 Size: 1770 125 226 152 2109 1697 Fragments arranged by size: 2109 1770 1697 226 152 125 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 3404 1870 Size: 73 1461 4545 Fragments arranged by size: 4545 1461 73 BamHI G'GATC_C Cuts at: 2301 2519 3409 3776 2301 Size: 218 890 367 4604 Fragments arranged by size: 4604 890 367 218 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2537 3400 4855 618 Size: 325 217 627 750 863 1455 1842 Fragments arranged by size: 1842 1455 863 750 627 325 217 BanII G_rGCy'C Cuts at: 1391 3398 1391 Size: 2007 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2862 928 Size: 971 963 4145 Fragments arranged by size: 4145 971 963 BbvCI CC'TCA_GC Cuts at: 2053 2053 Size: 6079 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2484 2518 3103 3137 4270 Size: 34 50 34 463 34 585 34 1133 Cuts at: 4270 4304 1903 Size: 34 3678 Fragments arranged by size: 3678 1133 585 463 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3971 5181 3971 Size: 1210 4869 BclI T'GATC_A Cuts at: 1548 2559 1548 Size: 1011 5068 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4690 136 Size: 107 122 71 631 605 3018 1525 Fragments arranged by size: 3018 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2972 6074 2972 Size: 3102 2977 BlpI GC'TnA_GC Cuts at: 3496 3496 Size: 6079 Bme1580I G_kGCm'C Cuts at: 1790 4140 5296 1790 Size: 2350 1156 2573 Fragments arranged by size: 2573 2350 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6079 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3810 4768 442 Size: 416 53 83 399 24 2393 958 1753 Fragments arranged by size: 2393 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4739 1239 Size: 3500 2579 Bpu10I CC'TnA_GC Cuts at: 2053 2053 Size: 6079 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3258 4204 5069 5367 3258 Size: 946 865 298 3970 Fragments arranged by size: 3970 946 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4742 882 Size: 617 3243 2219 Fragments arranged by size: 3243 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2676 2985 3870 493 Size: 711 1472 309 885 2702 Fragments arranged by size: 2702 1472 885 711 309 BsaBI GATnn'nnATC Cuts at: 6073 6073 Size: 6079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2472 2695 3886 4268 Size: 53 83 186 936 939 223 1191 382 Cuts at: 4268 4903 275 Size: 635 1451 Fragments arranged by size: 1451 1191 939 936 635 382 223 186 83 53 BsaWI w'CCGG_w Cuts at: 4512 4910 5184 5331 5742 4512 Size: 398 274 147 411 4849 Fragments arranged by size: 4849 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2441 2471 3147 3177 1178 Size: 30 165 30 1038 30 676 30 4080 Fragments arranged by size: 4080 1038 676 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 3104 1186 Size: 904 1014 4161 Fragments arranged by size: 4161 1014 904 BseYI C'CCAG_C Cuts at: 2193 5282 5778 2193 Size: 3089 496 2494 Fragments arranged by size: 3089 2494 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2446 1163 Size: 573 710 4796 Fragments arranged by size: 4796 710 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2374 2494 3529 4290 5318 1540 Size: 275 186 373 120 1035 761 1028 2301 Fragments arranged by size: 2301 1035 1028 761 373 275 186 120 BsiHKAI G_wGCw'C Cuts at: 4140 4225 5296 4140 Size: 85 1071 4923 Fragments arranged by size: 4923 1071 85 BsmI GAATG_Cn' Cuts at: 2623 3590 3683 2623 Size: 967 93 5019 Fragments arranged by size: 5019 967 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2686 2686 Size: 6079 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3398 4140 4225 5296 1391 Size: 399 1608 742 85 1071 2174 Fragments arranged by size: 2174 1608 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5742 5742 Size: 6079 BspHI T'CATG_A Cuts at: 2913 3968 5698 2913 Size: 1055 1730 3294 Fragments arranged by size: 3294 1730 1055 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2910 3434 844 Size: 2066 524 3489 Fragments arranged by size: 3489 2066 524 BsrBI CCG'CTC Cuts at: 1394 1823 3966 1394 Size: 429 2143 3507 Fragments arranged by size: 3507 2143 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4574 4748 65 Size: 1183 3326 174 1396 Fragments arranged by size: 3326 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4723 4910 1700 Size: 110 2913 187 2869 Fragments arranged by size: 2913 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6079 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6079 BssSI C'ACGA_G Cuts at: 4139 5151 4139 Size: 1012 5067 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6079 BstBI TT'CG_AA Cuts at: 2379 4883 2379 Size: 2504 3575 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6079 BstYI r'GATC_y Cuts at: 1972 2301 2519 2972 3409 3776 4161 4178 5630 Size: 329 218 453 437 367 385 17 1452 Cuts at: 5630 5716 5728 6074 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 453 437 385 367 346 329 218 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2466 2541 2994 513 Size: 552 1401 75 453 3598 Fragments arranged by size: 3598 1401 552 453 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5340 Fragments arranged by size: 5340 683 56 BtsI GCAGTG_nn' Cuts at: 3595 4389 4409 3595 Size: 794 20 5265 Fragments arranged by size: 5265 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5086 809 Size: 507 3770 1802 Fragments arranged by size: 3770 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2371 3526 4415 Size: 54 1425 50 275 18 541 1155 889 Cuts at: 4415 6044 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1155 889 541 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2371 3526 1537 Size: 275 559 1155 4090 Fragments arranged by size: 4090 1155 559 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4009 1353 Size: 2656 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2126 4666 5040 5186 387 Size: 1064 138 537 2540 374 146 1280 Fragments arranged by size: 2540 1280 1064 537 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4142 4739 5526 5794 1239 Size: 393 2510 597 787 268 1524 Fragments arranged by size: 2510 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3388 3766 3388 Size: 378 5701 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3034 3034 Size: 6079 FspI TGC'GCA Cuts at: 4585 4585 Size: 6079 HaeII r_GCGC'y Cuts at: 1678 5226 1678 Size: 3548 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3056 3088 4720 4752 4794 Size: 32 135 32 1484 32 1632 32 42 Cuts at: 4794 4826 1373 Size: 32 2626 Fragments arranged by size: 2626 1632 1484 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2548 2575 3098 3423 3669 669 Size: 988 281 610 27 523 325 246 3079 Fragments arranged by size: 3079 988 610 523 325 281 246 27 HindIII A'AGCT_T Cuts at: 748 748 Size: 6079 HpaI GTT'AAC Cuts at: 3669 3669 Size: 6079 KpnI G_GTAC'C Cuts at: 3404 3404 Size: 6079 MfeI C'AATT_G Cuts at: 3678 3678 Size: 6079 MluI A'CGCG_T Cuts at: 5955 5955 Size: 6079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5193 5377 1305 Size: 3888 184 2007 Fragments arranged by size: 3888 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4257 Fragments arranged by size: 4257 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2154 4037 4396 4555 518 Size: 648 988 1883 359 159 2042 Fragments arranged by size: 2042 1883 988 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2224 3495 4172 5320 5565 1606 Size: 618 1271 677 1148 245 2120 Fragments arranged by size: 2120 1271 1148 677 618 245 NcoI C'CATG_G Cuts at: 513 1065 2466 2541 2994 513 Size: 552 1401 75 453 3598 Fragments arranged by size: 3598 1401 552 453 75 NdeI CA'TA_TG Cuts at: 387 387 Size: 6079 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6079 NotI GC'GGCC_GC Cuts at: 3526 3526 Size: 6079 NspI r_CATG'y Cuts at: 1132 1229 3221 3437 4982 1132 Size: 97 1992 216 1545 2229 Fragments arranged by size: 2229 1992 1545 216 97 PasI CC'CwG_GG Cuts at: 1348 3003 1348 Size: 1655 4424 PciI A'CATG_T Cuts at: 3217 4978 3217 Size: 1761 4318 PflMI CCAn_nnn'nTGG Cuts at: 1108 2241 2995 1108 Size: 1133 754 4192 Fragments arranged by size: 4192 1133 754 PfoI T'CCnGG_A Cuts at: 2522 2522 Size: 6079 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3147 3179 4148 4180 5661 5693 3147 Size: 32 969 32 1481 32 3533 Fragments arranged by size: 3533 1481 969 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2399 1613 Size: 786 5293 PsiI TTA'TAA Cuts at: 3649 3649 Size: 6079 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2891 2923 3261 3293 2891 Size: 32 338 32 5677 Fragments arranged by size: 5677 338 32 32 PstI C_TGCA'G Cuts at: 830 3027 3431 830 Size: 2197 404 3478 Fragments arranged by size: 3478 2197 404 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 PvuII CAG'CTG Cuts at: 2224 2224 Size: 6079 SalI G'TCGA_C Cuts at: 1936 2573 3421 1936 Size: 637 848 4594 Fragments arranged by size: 4594 848 637 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 4327 6039 4327 Size: 1712 4367 SfcI C'TryA_G Cuts at: 826 1045 3023 3427 4562 5243 5434 826 Size: 219 1978 404 1135 681 191 1471 Fragments arranged by size: 1978 1471 1135 681 404 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6079 SmaI CCC'GGG Cuts at: 1872 3406 1872 Size: 1534 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3273 4183 5084 5346 820 Size: 197 926 1330 910 901 262 1553 Fragments arranged by size: 1553 1330 926 910 901 262 197 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 3437 1132 Size: 2305 3774 SspI AAT'ATT Cuts at: 5 52 4003 5 Size: 47 3951 2081 Fragments arranged by size: 3951 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6079 StyI C'CwwG_G Cuts at: 513 1065 2038 2116 2397 2466 2541 2994 3518 Size: 552 973 78 281 69 75 453 524 Cuts at: 3518 513 Size: 3074 Fragments arranged by size: 3074 973 552 524 453 281 78 75 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2508 2803 3300 4106 4123 4276 4461 Size: 1255 928 295 497 806 17 153 185 Cuts at: 4461 4906 325 Size: 445 1498 Fragments arranged by size: 1498 1255 928 806 497 445 295 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2655 3250 4325 Size: 274 80 33 51 1443 678 595 1075 Cuts at: 4325 6037 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1075 678 595 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6039 6071 6039 Size: 32 6047 Tth111I GACn'n_nGTC Cuts at: 1316 2929 1316 Size: 1613 4466 XbaI T'CTAG_A Cuts at: 3415 3415 Size: 6079 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2815 2815 Size: 6079 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6079 XmaI C'CCGG_G Cuts at: 1870 3404 1870 Size: 1534 4545 XmnI GAAnn'nnTTC Cuts at: 3162 4208 3162 Size: 1046 5033 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2473 3887 276 Size: 53 83 186 936 939 1414 2468 Fragments arranged by size: 2468 1414 939 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI