################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MseI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AocI 582 CCTGAGG CC^T[ACGT]AGG AspI 187 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 196 TGGCCA TGG^CCA BbsI 133 GTCTTC GAAGAC(2/6) BglI 251 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 420 GCTGAGC GC^T[ACGT]AGC BmyI 3 GTGCCC G[AGT]GC[ACT]^C BpmI 94 CTGGAG CTGGAG(16/14) Bpu1102I 420 GCTGAGC GC^T[ACGT]AGC BpuAI 133 GTCTTC GAAGAC(2/6) BsmFI 88 GGGAC GGGAC(10/14) Bsp1286I 3 GTGCCC G[AGT]GC[ACT]^C BsrBI 145 GAGCGG CCGCTC(-3/-3) BsrFI 290 GCCGGT [GA]^CCGG[CT] BssSI 523 CTCGTG CACGAG(-5/-1) BstBI 77 TTCGAA TT^CGAA BstYI 440 AGATCC [GA]^GATC[CT] Bsu36I 582 CCTGAGG CC^T[ACGT]AGG CelII 420 GCTGAGC GC^T[ACGT]AGC CfoI 418 GCGC GCG^C Cfr10I 290 GCCGGT [GA]^CCGG[CT] DraII 88 GGGACCC [GA]G^G[ACGT]CC[CT] EaeI 196 TGGCCA [CT]^GGCC[GA] Eco81I 582 CCTGAGG CC^T[ACGT]AGG EcoNI 522 CCTCGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 88 GGGACCC [GA]G^G[ACGT]CC[CT] FauI 44 CCCGC CCCGC(4/6) HaeII 417 GGCGCT [GA]GCGC^[CT] HhaI 418 GCGC GCG^C HinP1I 418 GCGC G^CGC HinfI 358 GACTC G^A[ACGT]TC MaeII 269 ACGT A^CGT MflI 440 AGATCC [GA]^GATC[CT] MluNI 196 TGGCCA TGG^CCA MscI 196 TGGCCA TGG^CCA NspV 77 TTCGAA TT^CGAA PaeR7I 83 CTCGAG C^TCGAG PflFI 187 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 219 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 358 GACTC GAGTC(4/5) PpuMI 88 GGGACCC [GA]G^G[AT]CC[CT] SfuI 77 TTCGAA TT^CGAA Tth111I 187 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 219 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 479 CCACCCTGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 83 CTCGAG C^TCGAG XhoII 440 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 487 GTAC GT^AC 507 GTAC AlwI 441 GATCC GGATC(4/5) 473 GATCC AlwNI 589 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 619 CAGGGTCTG AvaI 83 CTCGAG C^[CT]CG[GA]G 307 CCCGAG AvaII 89 GGACC G^G[AT]CC 603 GGACC BsaI 215 GAGACC GGTCTC(1/5) 68 GGTCTC BseRI 151 CTCCTC GAGGAG(10/8) 97 GAGGAG BsmAI 215 GAGAC GTCTC(1/5) 69 GTCTC BsoBI 83 CTCGAG C^[CT]CG[GA]G 307 CCCGAG BsrI 14 ACTGG ACTGG(1/-1) 489 ACTGG DdeI 421 CTGAG C^T[ACGT]AG 583 CTGAG DpnII 441 GATC ^GATC 473 GATC MaeIII 11 GTAAC ^GT[ACGT]AC 176 GTAAC MboI 441 GATC ^GATC 473 GATC MboII 438 GAAGA GAAGA(8/7) 134 TCTTC NdeII 441 GATC ^GATC 473 GATC RsaI 487 GTAC GT^AC 507 GTAC Sau3AI 441 GATC ^GATC 473 GATC SmlI 63 CTCAAG C^T[CT][GA]AG 83 CTCGAG StuI 201 AGGCCT AGG^CCT 311 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI BbvI BcnI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI MnlI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI TaqI TseI TthHB8I