################################## # NO CUT ENZYMES ( 199 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AluI 458 AGCT AG^CT AspHI 322 GTGCTC G[AT]GC[AT]^C AviII 277 TGCGCA TGC^GCA BbvI 167 GCTGC GCAGC(8/12) BfaI 413 CTAG C^TAG BlpI 150 GCTCAGC GC^T[ACGT]AGC BmyI 322 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 150 GCTCAGC GC^T[ACGT]AGC BsaJI 39 CCTGGG C^C[ACGT][ACGT]GG BsiHKAI 322 GTGCTC G[AT]GC[AT]^C BsiYI 39 CCTGGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 39 CCTGGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 322 GTGCTC G[AGT]GC[ACT]^C BspMI 274 ACCTGC ACCTGC(4/8) BsrBI 100 CCGCTC CCGCTC(-3/-3) BsrDI 408 CATTGC GCAATG(2/0) BstXI 7 CCATTCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 320 GCGTGC GC[ACGT]^[ACGT]GC CelII 150 GCTCAGC GC^T[ACGT]AGC CfoI 278 GCGC GCG^C DraIII 200 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG Fnu4HI 167 GCTGC GC^[ACGT]GC FspI 277 TGCGCA TGC^GCA HhaI 278 GCGC GCG^C HinP1I 278 GCGC G^CGC HinfI 444 GATTC G^A[ACGT]TC ItaI 167 GCTGC GC^[ACGT]GC MaeI 413 CTAG C^TAG MseI 236 TTAA T^TAA MwoI 380 GCTGTGTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 191 GGAACC GG[ACGT]^[ACGT]CC TaqI 70 TCGA T^CGA TfiI 444 GATTC G^A[AT]TC Tru9I 236 TTAA T^TAA TseI 167 GCTGC G^C[AT]GC Tsp509I 260 AATT ^AATT TspRI 13 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 70 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BbsI 292 GAAGAC GAAGAC(2/6) 142 GTCTTC BpuAI 292 GAAGAC GAAGAC(2/6) 142 GTCTTC BsmFI 42 GGGAC GGGAC(10/14) 416 GTCCC BsrI 45 ACTGG ACTGG(1/-1) 12 CCAGT BssKI 39 CCTGG ^CC[ACGT]GG 195 CCAGG BstF5I 309 GGATG GGATG(2/0) 453 GGATG BstNI 39 CCTGG CC^[AT]GG 195 CCAGG DdeI 151 CTCAG C^T[ACGT]AG 420 CTCAG EcoRII 39 CCTGG ^CC[AT]GG 195 CCAGG FokI 309 GGATG GGATG(9/13) 453 GGATG HaeIII 37 GGCC GG^CC 426 GGCC MslI 8 CATTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 108 CATCTATATG MvaI 39 CCTGG CC^[AT]GG 195 CCAGG NlaIII 147 CATG CATG^ 393 CATG ScrFI 39 CCTGG CC^[ACGT]GG 195 CCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### AciI HphI MaeIII MboII MnlI SfaNI