################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI BamHI BbeI BbrPI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoT22I FbaI FseI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 619 GTCTAC GT^[AC][GT]AC AcsI 1192 AAATTT [GA]^AATT[CT] AcyI 1408 GACGCC G[GA]^CG[CT]C AgeI 57 ACCGGT A^CCGGT Aor51HI 564 AGCGCT AGC^GCT ApoI 1192 AAATTT [GA]^AATT[CT] AspHI 553 GTGCTC G[AT]GC[AT]^C AspI 1269 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1124 TGCGCA TGC^GCA AvrII 264 CCTAGG C^CTAGG BanI 54 GGCACC G^G[CT][GA]CC BanII 570 GGGCTC G[GA]GC[CT]^C BbvCI 1361 CCTCAGC CCTCAGC(-5/-2) BcgI 824 GCATCGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 264 CCTAGG C^CTAGG BsaHI 1408 GACGCC G[GA]^CG[CT]C BsaWI 57 ACCGGT [AT]^CCGG[AT] BsiEI 366 CGACCG CG[GA][CT]^CG BsiHKAI 553 GTGCTC G[AT]GC[AT]^C BsmFI 1381 GGGAC GGGAC(10/14) DraI 922 TTTAAA TTT^AAA DrdI 669 GACCGACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 564 AGCGCT AGC^GCT EcoRV 944 GATATC GAT^ATC FspI 1124 TGCGCA TGC^GCA Hin1I 1408 GACGCC G[GA]^CG[CT]C HincII 196 GTCAAC GT[CT]^[GA]AC HindII 196 GTCAAC GT[CT]^[GA]AC MseI 923 TTAA T^TAA NspI 1059 ACATGC [GA]CATG^[CT] PflFI 1269 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1092 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 57 ACCGGT A^CCGGT PleI 682 GACTC GAGTC(4/5) PpuMI 260 AGGTCCT [GA]G^G[AT]CC[CT] SapI 555 GCTCTTC GCTCTTC(1/4) SgrAI 56 CACCGGTG C[GA]^CCGG[CT]G StuI 1004 AGGCCT AGG^CCT Tru9I 923 TTAA T^TAA Tsp509I 1193 AATT ^AATT Tth111I 1269 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1092 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccII 333 CGCG CG^CG 616 CGCG AfaI 609 GTAC GT^AC 1256 GTAC Asp700I 17 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1191 GAAATTTTTC AvaII 261 GGTCC G^G[AT]CC 478 GGACC BbsI 289 GTCTTC GAAGAC(2/6) 1050 GTCTTC BfaI 136 CTAG C^TAG 265 CTAG BpuAI 289 GTCTTC GAAGAC(2/6) 1050 GTCTTC BsaAI 550 TACGTG [CT]AC^GT[GA] 622 TACGTG BspMI 167 ACCTGC ACCTGC(4/8) 581 ACCTGC BsrI 272 ACTGG ACTGG(1/-1) 1029 CCAGT BstUI 333 CGCG CG^CG 616 CGCG BstYI 1160 GGATCT [GA]^GATC[CT] 1216 GGATCT EarI 556 CTCTTC CTCTTC(1/4) 1200 CTCTTC EcoNI 278 CCTTCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1151 CCTTCTACAGG EcoT14I 71 CCAAGG C^C[AT][AT]GG 264 CCTAGG HaeII 564 AGCGCT [GA]GCGC^[CT] 748 AGCGCC Ksp632I 556 CTCTTC CTCTTC(1/4) 1200 CTCTTC MaeI 136 CTAG C^TAG 265 CTAG MaeII 551 ACGT A^CGT 623 ACGT MflI 1160 GGATCT [GA]^GATC[CT] 1216 GGATCT MvnI 333 CGCG CG^CG 616 CGCG NaeI 51 GCCGGC GCC^GGC 501 GCCGGC NgoMIV 51 GCCGGC G^CCGGC 501 GCCGGC PvuII 954 CAGCTG CAG^CTG 1376 CAGCTG RsaI 609 GTAC GT^AC 1256 GTAC SfcI 1155 CTACAG C^T[GA][CT]AG 1385 CTACAG SmaI 794 CCCGGG CCC^GGG 1338 CCCGGG StyI 71 CCAAGG C^C[AT][AT]GG 264 CCTAGG TaqI 257 TCGA T^CGA 365 TCGA TfiI 1025 GAATC G^A[AT]TC 1351 GATTC TthHB8I 257 TCGA T^CGA 365 TCGA XcmI 1055 CCAAACATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1088 CCAGCCAGGCCGTGG XhoII 1160 GGATCT [GA]^GATC[CT] 1216 GGATCT XmaCI 794 CCCGGG C^CCGGG 1338 CCCGGG XmaI 794 CCCGGG C^CCGGG 1338 CCCGGG XmnI 17 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1191 GAAATTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AluI AlwI AvaI BalI BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SmlI TseI Tsp45I TspRI