Restriction map for
pFN21AE3419
(Circular) (Six-base) MAPSORT of: pFN21AE3419.seq Check: 9166 from: 1 to: 6130
pFN21AE3419 6130 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2181 2181
Size: 6130
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2625 3473 1937
Size: 688 848 4594
Fragments arranged by size:
4594 848 688
Acc65I G'GTAC_C
Cuts at: 3451 3451
Size: 6130
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4193 5577 5845 1632
Size: 2561 1384 268 1917
Fragments arranged by size:
2561 1917 1384 268
AfeI AGC'GCT
Cuts at: 2571 2571
Size: 6130
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 5029 6006 1203
Size: 3826 977 1327
Fragments arranged by size:
3826 1327 977
AgeI A'CCGG_T
Cuts at: 2062 4961 2062
Size: 2899 3231
AhdI GACnn_n'nnGTC
Cuts at: 4859 4859
Size: 6130
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6130
AlwNI CAG_nnn'CTG
Cuts at: 3873 5445 3873
Size: 1572 4558
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 3197 3439 3665 3817 5926 1493
Size: 1704 242 226 152 2109 1697
Fragments arranged by size:
2109 1704 1697 242 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 2017 2090 2513 2799 3343 3455 1870
Size: 73 74 73 423 286 544 112 4545
Fragments arranged by size:
4545 544 423 286 112 74 73 73
AvrII C'CTAG_G
Cuts at: 2269 2269
Size: 6130
BamHI G'GATC_C
Cuts at: 3460 3827 3460
Size: 367 5763
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 3451 4906 618
Size: 325 217 627 272 1392 1455 1842
Fragments arranged by size:
1842 1455 1392 627 325 272 217
BanII G_rGCy'C
Cuts at: 1391 2579 3449 1391
Size: 1188 870 4072
Fragments arranged by size:
4072 1188 870
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2287 3048 928
Size: 971 388 761 4010
Fragments arranged by size:
4010 971 761 388
BbvCI CC'TCA_GC
Cuts at: 3367 3367
Size: 6130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2818 2852 4321 4355 1903
Size: 34 881 34 1469 34 3678
Fragments arranged by size:
3678 1469 881 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4022 5232 4022
Size: 1210 4920
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4741 136
Size: 107 122 71 631 605 3069 1525
Fragments arranged by size:
3069 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6125 6125
Size: 6130
BlpI GC'TnA_GC
Cuts at: 3547 3547
Size: 6130
Bme1580I G_kGCm'C
Cuts at: 1790 2461 4191 5347 1790
Size: 671 1730 1156 2573
Fragments arranged by size:
2573 1730 1156 671
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6130
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2287 3861 4819
Size: 416 53 83 399 24 870 1574 958
Cuts at: 4819 442
Size: 1753
Fragments arranged by size:
1753 1574 958 870 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2518 2974 3320 4790 1239
Size: 1279 456 346 1470 2579
Fragments arranged by size:
2579 1470 1279 456 346
Bpu10I CC'TnA_GC
Cuts at: 3367 3367
Size: 6130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2642 2654 2774 3046 4255 5120 5418 2642
Size: 12 120 272 1209 865 298 3354
Fragments arranged by size:
3354 1209 865 298 272 120 12
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2438 2516 3146 4793 882
Size: 617 939 78 630 1647 2219
Fragments arranged by size:
2219 1647 939 630 617 78
BsaAI yAC'GTr
Cuts at: 493 1204 2557 2629 3921 493
Size: 711 1353 72 1292 2702
Fragments arranged by size:
2702 1353 1292 711 72
BsaBI GATnn'nnATC
Cuts at: 6124 6124
Size: 6130
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3414 3937 4319 4954
Size: 53 83 186 936 1881 523 382 635
Cuts at: 4954 275
Size: 1451
Fragments arranged by size:
1881 1451 936 635 523 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2062 4563 4961 5235 5382 5793 2062
Size: 2501 398 274 147 411 2399
Fragments arranged by size:
2501 2399 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2972 3002 3152 3182 3239
Size: 30 165 30 1569 30 150 30 57
Cuts at: 3239 3269 1178
Size: 30 4039
Fragments arranged by size:
4039 1569 165 150 57 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2440 2558 2777 1186
Size: 1254 118 219 4539
Fragments arranged by size:
4539 1254 219 118
BseYI C'CCAG_C
Cuts at: 2128 2572 2824 3092 3383 3425 5333 5829 2128
Size: 444 252 268 291 42 1908 496 2429
Fragments arranged by size:
2429 1908 496 444 291 268 252 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2029 2159 2317 3068 3325 1163
Size: 573 293 130 158 751 257 3968
Fragments arranged by size:
3968 751 573 293 257 158 130
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2374 3580 4341 5369 1540
Size: 275 186 373 1206 761 1028 2301
Fragments arranged by size:
2301 1206 1028 761 373 275 186
BsiHKAI G_wGCw'C
Cuts at: 2562 4191 4276 5347 2562
Size: 1629 85 1071 3345
Fragments arranged by size:
3345 1629 1071 85
BsmI GAATG_Cn'
Cuts at: 3641 3734 3641
Size: 93 6037
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2461 2562 2579 3449 4191 4276 5347
Size: 399 671 101 17 870 742 85 1071
Cuts at: 5347 1391
Size: 2174
Fragments arranged by size:
2174 1071 870 742 671 399 101 85
17
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 4019 5749 4019
Size: 1730 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2181 2595 3485 844
Size: 1337 414 890 3489
Fragments arranged by size:
3489 1337 890 414
BsrBI CCG'CTC
Cuts at: 1394 1823 4017 1394
Size: 429 2194 3507
Fragments arranged by size:
3507 2194 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4625 4799 65
Size: 1183 3377 174 1396
Fragments arranged by size:
3377 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2056 2062 2506 4774 4961 1700
Size: 110 246 6 444 2268 187 2869
Fragments arranged by size:
2869 2268 444 246 187 110 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6130
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 4190 5202 4190
Size: 1012 5118
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6130
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6130
BstXI CCAn_nnnn'nTGG
Cuts at: 2605 2824 3383 2605
Size: 219 559 5352
Fragments arranged by size:
5352 559 219
BstYI r'GATC_y
Cuts at: 1972 3165 3221 3460 3827 4212 4229 5681 5767
Size: 1193 56 239 367 385 17 1452 86
Cuts at: 5767 5779 6125 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1193 385 367 346 239 86
56 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2235 3102 3138 3217 513
Size: 552 939 231 867 36 79 3426
Fragments arranged by size:
3426 939 867 552 231 79 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 2322 3313 3325 3646 4440 4460 2322
Size: 991 12 321 794 20 3992
Fragments arranged by size:
3992 991 794 321 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6095
DraI TTT'AAA
Cuts at: 2929 3434 2929
Size: 505 5625
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2680 5137 809
Size: 507 1364 2457 1802
Fragments arranged by size:
2457 1802 1364 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 2595 2634
Size: 54 1425 50 275 18 384 381 39
Cuts at: 2634 2748 2814 3577 4466 6095 8
Size: 114 66 763 889 1629 43
Fragments arranged by size:
1629 1425 889 763 384 381 275 114
66 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2567 3211 4060 1353
Size: 1214 644 849 3423
Fragments arranged by size:
3423 1214 849 644
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2242 3326 4717 5091 5237 387
Size: 1064 138 653 1084 1391 374 146 1280
Fragments arranged by size:
1391 1280 1084 1064 653 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2518 2974 3320 4193 4790 5577 5845
Size: 393 886 456 346 873 597 787 268
Cuts at: 5845 1239
Size: 1524
Fragments arranged by size:
1524 886 873 787 597 456 393 346
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2287 3160 2287
Size: 873 5257
EcoO109I rG'GnC_Cy
Cuts at: 2107 2266 2653 2107
Size: 159 387 5584
Fragments arranged by size:
5584 387 159
EcoRI G'AATT_C
Cuts at: 3439 3817 3439
Size: 378 5752
EcoRV GAT'ATC
Cuts at: 2951 2951
Size: 6130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2788 2788
Size: 6130
FspI TGC'GCA
Cuts at: 3131 4636 3131
Size: 1505 4625
FspAI rTGC'GCAy
Cuts at: 3131 3131
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 2573 2757 5277 1678
Size: 895 184 2520 2531
Fragments arranged by size:
2531 2520 895 184
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2416 2448 3239 3265 3271
Size: 32 135 32 844 32 791 26 6
Cuts at: 3271 3297 3307 3339 4771 4803 4845 4877 1373
Size: 26 10 32 1432 32 42 32 2626
Fragments arranged by size:
2626 1432 844 791 135 42 32 32
32 32 32 32 26 26 10 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2203 3474 3720 669
Size: 988 281 265 1271 246 3079
Fragments arranged by size:
3079 1271 988 281 265 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6130
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KpnI G_GTAC'C
Cuts at: 3455 3455
Size: 6130
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2055 2064 2664 5244 5428 1305
Size: 750 9 600 2580 184 2007
Fragments arranged by size:
2580 2007 750 600 184 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2597 2636 2750 10
Size: 54 1425 343 765 39 114 3390
Fragments arranged by size:
3390 1425 765 343 114 54 39
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2384 3137 4088 4447 4606 518
Size: 648 975 243 753 951 359 159 2042
Fragments arranged by size:
2042 975 951 753 648 359 243 159
MspA1I CmG'CkG
Cuts at: 1606 2398 2742 2961 3383 3546 4223 5371 5616
Size: 792 344 219 422 163 677 1148 245
Cuts at: 5616 1606
Size: 2120
Fragments arranged by size:
2120 1148 792 677 422 344 245 219
163
NaeI GCC'GGC
Cuts at: 2058 2508 2058
Size: 450 5680
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4639
Fragments arranged by size:
4639 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6130
NgoMIV G'CCGG_C
Cuts at: 2056 2506 2056
Size: 450 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 3068 3488 5033 1132
Size: 97 1839 420 1545 2229
Fragments arranged by size:
2229 1839 1545 420 97
PasI CC'CwG_GG
Cuts at: 1348 3070 1348
Size: 1722 4408
PciI A'CATG_T
Cuts at: 5029 5029
Size: 6130
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3103 1108
Size: 1995 4135
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2266 2266
Size: 6130
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PstI C_TGCA'G
Cuts at: 830 3482 830
Size: 2652 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
PvuII CAG'CTG
Cuts at: 2961 3383 2961
Size: 422 5708
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SapI GCTCTTCn'nnn_
Cuts at: 2567 2567
Size: 6130
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SfcI C'TryA_G
Cuts at: 826 1045 3160 3390 3478 4613 5294 5485 826
Size: 219 2115 230 88 1135 681 191 1471
Fragments arranged by size:
2115 1471 1135 681 230 219 191 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 2062 1700
Size: 362 5768
SmaI CCC'GGG
Cuts at: 1872 2801 3345 3457 1872
Size: 929 544 112 4545
Fragments arranged by size:
4545 929 544 112
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2657 2669 2789 3025 4234 5135
Size: 197 926 714 12 120 236 1209 901
Cuts at: 5135 5397 820
Size: 262 1553
Fragments arranged by size:
1553 1209 926 901 714 262 236 197
120 12
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 4054 5
Size: 47 4002 2081
Fragments arranged by size:
4002 2081 47
StuI AGG'CCT
Cuts at: 1517 3011 1517
Size: 1494 4636
StyI C'CwwG_G
Cuts at: 513 1065 2004 2076 2269 3569 513
Size: 552 939 72 193 1300 3074
Fragments arranged by size:
3074 1300 939 552 193 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2063 2435 2690 4157 4174 4327 4512
Size: 1255 483 372 255 1467 17 153 185
Cuts at: 4512 4957 325
Size: 445 1498
Fragments arranged by size:
1498 1467 1255 483 445 372 255 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2613 4376 6088
Size: 274 80 33 51 1443 636 1763 1712
Cuts at: 6088 96
Size: 138
Fragments arranged by size:
1763 1712 1443 636 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2312 2344 6090 6122 2312
Size: 32 3746 32 2320
Fragments arranged by size:
3746 2320 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3277 1316
Size: 1961 4169
XbaI T'CTAG_A
Cuts at: 3466 3466
Size: 6130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3067 3100 3067
Size: 33 6097
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 2799 3343 3455 1870
Size: 929 544 112 4545
Fragments arranged by size:
4545 929 544 112
XmnI GAAnn'nnTTC
Cuts at: 2026 3200 4259 2026
Size: 1174 1059 3897
Fragments arranged by size:
3897 1174 1059
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI KasI NarI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI