(Linear) (Six-base) MAPSORT of: KIEE3419.seq Check: 6950 from: 1 to: 1425 KIEE3419.orf With 182 enzymes: SgfI * January 29, 2014 11:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 176 1425 Size: 176 1249 AccI GT'mk_AC Cuts at: 0 620 1425 Size: 620 805 AfeI AGC'GCT Cuts at: 0 566 1425 Size: 566 859 AgeI A'CCGG_T Cuts at: 0 57 1425 Size: 57 1368 ApoI r'AATT_y Cuts at: 0 1192 1425 Size: 1192 233 AvaI C'yCGr_G Cuts at: 0 12 85 508 794 1338 1425 Size: 12 73 423 286 544 87 Fragments arranged by size: 544 423 286 87 73 12 AvrII C'CTAG_G Cuts at: 0 264 1425 Size: 264 1161 BanI G'GyrC_C Cuts at: 0 54 1425 Size: 54 1371 BanII G_rGCy'C Cuts at: 0 574 1425 Size: 574 851 BbsI GAAGACnn'nnnn_ Cuts at: 0 282 1043 1425 Size: 282 761 382 Fragments arranged by size: 761 382 282 BbvCI CC'TCA_GC Cuts at: 0 1362 1425 Size: 1362 63 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 813 847 1425 Size: 813 34 578 Fragments arranged by size: 813 578 34 Bme1580I G_kGCm'C Cuts at: 0 456 1425 Size: 456 969 BmrI ACTGGGnnnn_n' Cuts at: 0 282 1425 Size: 282 1143 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 513 969 1315 1425 Size: 513 456 346 110 Fragments arranged by size: 513 456 346 110 Bpu10I CC'TnA_GC Cuts at: 0 1362 1425 Size: 1362 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 637 649 769 1041 1425 Size: 637 12 120 272 384 Fragments arranged by size: 637 384 272 120 12 BsaI GGTCTCn'nnnn_ Cuts at: 0 433 511 1141 1425 Size: 433 78 630 284 Fragments arranged by size: 630 433 284 78 BsaAI yAC'GTr Cuts at: 0 552 624 1425 Size: 552 72 801 Fragments arranged by size: 801 552 72 BsaHI Gr'CG_yC Cuts at: 0 1409 1425 Size: 1409 16 BsaWI w'CCGG_w Cuts at: 0 57 1425 Size: 57 1368 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 967 997 1147 1177 1234 1264 1425 Size: 967 30 150 30 57 30 161 Fragments arranged by size: 967 161 150 57 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 435 553 772 1425 Size: 435 118 219 653 Fragments arranged by size: 653 435 219 118 BseYI C'CCAG_C Cuts at: 0 123 567 819 1087 1378 1420 1425 Size: 123 444 252 268 291 42 5 Fragments arranged by size: 444 291 268 252 123 42 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 24 154 312 1063 1320 1425 Size: 24 130 158 751 257 105 Fragments arranged by size: 751 257 158 130 105 24 BsiEI CG_ry'CG Cuts at: 0 369 1425 Size: 369 1056 BsiHKAI G_wGCw'C Cuts at: 0 557 1425 Size: 557 868 Bsp1286I G_dGCh'C Cuts at: 0 456 557 574 1425 Size: 456 101 17 851 Fragments arranged by size: 851 456 101 17 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 176 590 1425 Size: 176 414 835 Fragments arranged by size: 835 414 176 BsrFI r'CCGG_y Cuts at: 0 51 57 501 1425 Size: 51 6 444 924 Fragments arranged by size: 924 444 51 6 BstXI CCAn_nnnn'nTGG Cuts at: 0 600 819 1378 1425 Size: 600 219 559 47 Fragments arranged by size: 600 559 219 47 BstYI r'GATC_y Cuts at: 0 1160 1216 1425 Size: 1160 56 209 Fragments arranged by size: 1160 209 56 BtgI C'CryG_G Cuts at: 0 230 1097 1133 1212 1425 Size: 230 867 36 79 213 Fragments arranged by size: 867 230 213 79 36 BtsI GCAGTG_nn' Cuts at: 0 317 1308 1320 1425 Size: 317 991 12 105 Fragments arranged by size: 991 317 105 12 DraI TTT'AAA Cuts at: 0 924 1425 Size: 924 501 DrdI GACnn_nn'nnGTC Cuts at: 0 675 1425 Size: 675 750 EaeI y'GGCC_r Cuts at: 0 209 590 629 743 809 1425 Size: 209 381 39 114 66 616 Fragments arranged by size: 616 381 209 114 66 39 EarI CTCTTCn'nnn_ Cuts at: 0 562 1206 1425 Size: 562 644 219 Fragments arranged by size: 644 562 219 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 237 1321 1425 Size: 237 1084 104 Fragments arranged by size: 1084 237 104 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 513 969 1315 1425 Size: 513 456 346 110 Fragments arranged by size: 513 456 346 110 EcoNI CCTnn'n_nnAGG Cuts at: 0 282 1155 1425 Size: 282 873 270 Fragments arranged by size: 873 282 270 EcoO109I rG'GnC_Cy Cuts at: 0 102 261 648 1425 Size: 102 159 387 777 Fragments arranged by size: 777 387 159 102 EcoRV GAT'ATC Cuts at: 0 946 1425 Size: 946 479 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 783 1425 Size: 783 642 FspI TGC'GCA Cuts at: 0 1126 1425 Size: 1126 299 FspAI rTGC'GCAy Cuts at: 0 1126 1425 Size: 1126 299 HaeII r_GCGC'y Cuts at: 0 568 752 1425 Size: 568 184 673 Fragments arranged by size: 673 568 184 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 411 443 1234 1260 1266 1292 1302 1334 Size: 411 32 791 26 6 26 10 32 Cuts at: 1334 1425 Size: 91 Fragments arranged by size: 791 411 91 32 32 26 26 10 6 HincII GTy'rAC Cuts at: 0 198 1425 Size: 198 1227 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 50 59 659 1425 Size: 50 9 600 766 Fragments arranged by size: 766 600 50 9 MscI TGG'CCA Cuts at: 0 592 631 745 1425 Size: 592 39 114 680 Fragments arranged by size: 680 592 114 39 MslI CAynn'nnrTG Cuts at: 0 136 379 1132 1425 Size: 136 243 753 293 Fragments arranged by size: 753 293 243 136 MspA1I CmG'CkG Cuts at: 0 393 737 956 1378 1425 Size: 393 344 219 422 47 Fragments arranged by size: 422 393 344 219 47 NaeI GCC'GGC Cuts at: 0 53 503 1425 Size: 53 450 922 Fragments arranged by size: 922 450 53 NgoMIV G'CCGG_C Cuts at: 0 51 501 1425 Size: 51 450 924 Fragments arranged by size: 924 450 51 NspI r_CATG'y Cuts at: 0 1063 1425 Size: 1063 362 PasI CC'CwG_GG Cuts at: 0 1065 1425 Size: 1065 360 PflMI CCAn_nnn'nTGG Cuts at: 0 1098 1425 Size: 1098 327 PpuMI rG'GwC_Cy Cuts at: 0 261 1425 Size: 261 1164 PvuII CAG'CTG Cuts at: 0 956 1378 1425 Size: 956 422 47 Fragments arranged by size: 956 422 47 SapI GCTCTTCn'nnn_ Cuts at: 0 562 1425 Size: 562 863 SfcI C'TryA_G Cuts at: 0 1155 1385 1425 Size: 1155 230 40 Fragments arranged by size: 1155 230 40 SgrAI Cr'CCGG_yG Cuts at: 0 57 1425 Size: 57 1368 SmaI CCC'GGG Cuts at: 0 796 1340 1425 Size: 796 544 85 Fragments arranged by size: 796 544 85 SmlI C'TyrA_G Cuts at: 0 652 664 784 1020 1425 Size: 652 12 120 236 405 Fragments arranged by size: 652 405 236 120 12 StuI AGG'CCT Cuts at: 0 1006 1425 Size: 1006 419 StyI C'CwwG_G Cuts at: 0 71 264 1425 Size: 71 193 1161 Fragments arranged by size: 1161 193 71 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 58 430 685 1425 Size: 58 372 255 740 Fragments arranged by size: 740 372 255 58 TatI w'GTAC_w Cuts at: 0 608 1425 Size: 608 817 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 307 339 1425 Size: 307 32 1086 Fragments arranged by size: 1086 307 32 Tth111I GACn'n_nGTC Cuts at: 0 1272 1425 Size: 1272 153 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1062 1095 1425 Size: 1062 33 330 Fragments arranged by size: 1062 330 33 XmaI C'CCGG_G Cuts at: 0 794 1338 1425 Size: 794 544 87 Fragments arranged by size: 794 544 87 XmnI GAAnn'nnTTC Cuts at: 0 21 1195 1425 Size: 21 1174 230 Fragments arranged by size: 1174 230 21 Enzymes that do cut: AarI AccI AfeI AgeI ApoI AvaI AvrII BanI BanII BbsI BbvCI BcgI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BstXI BstYI BtgI BtsI DraI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI FspI FspAI HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NgoMIV NspI PasI PflMI PpuMI PvuII SapI SfcI SgrAI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AcuI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EagI EcoICRI EcoRI FseI HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI ZraI