(Linear) (Six-base) MAPSORT of: KIEE6473.seq Check: 9708 from: 1 to: 1359 KIEE6473.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AccI GT'mk_AC Cuts at: 0 341 1359 Size: 341 1018 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 1147 1359 Size: 403 744 212 Fragments arranged by size: 744 403 212 AlwNI CAG_nnn'CTG Cuts at: 0 291 350 1092 1255 1359 Size: 291 59 742 163 104 Fragments arranged by size: 742 291 163 104 59 AvaI C'yCGr_G Cuts at: 0 64 637 671 1359 Size: 64 573 34 688 Fragments arranged by size: 688 573 64 34 AvrII C'CTAG_G Cuts at: 0 1240 1359 Size: 1240 119 BanI G'GyrC_C Cuts at: 0 168 1359 Size: 168 1191 BanII G_rGCy'C Cuts at: 0 548 1061 1359 Size: 548 513 298 Fragments arranged by size: 548 513 298 BbeI G_GCGC'C Cuts at: 0 172 1359 Size: 172 1187 BbsI GAAGACnn'nnnn_ Cuts at: 0 366 1359 Size: 366 993 BbvCI CC'TCA_GC Cuts at: 0 437 479 1359 Size: 437 42 880 Fragments arranged by size: 880 437 42 BciVI GTATCCnnnnn_n' Cuts at: 0 1161 1359 Size: 1161 198 BglI GCCn_nnn'nGGC Cuts at: 0 811 976 1359 Size: 811 165 383 Fragments arranged by size: 811 383 165 BlpI GC'TnA_GC Cuts at: 0 181 547 1359 Size: 181 366 812 Fragments arranged by size: 812 366 181 BmrI ACTGGGnnnn_n' Cuts at: 0 153 1359 Size: 153 1206 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 680 1159 1359 Size: 680 479 200 Fragments arranged by size: 680 479 200 Bpu10I CC'TnA_GC Cuts at: 0 437 479 1359 Size: 437 42 880 Fragments arranged by size: 880 437 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 376 838 1294 1359 Size: 376 462 456 65 Fragments arranged by size: 462 456 376 65 BsaI GGTCTCn'nnnn_ Cuts at: 0 35 696 854 1359 Size: 35 661 158 505 Fragments arranged by size: 661 505 158 35 BsaAI yAC'GTr Cuts at: 0 682 1359 Size: 682 677 BsaHI Gr'CG_yC Cuts at: 0 169 1359 Size: 169 1190 BsaWI w'CCGG_w Cuts at: 0 929 1359 Size: 929 430 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 76 106 1289 1319 1359 Size: 76 30 1183 30 40 Fragments arranged by size: 1183 76 40 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 88 97 132 454 496 507 1359 Size: 88 9 35 322 42 11 852 Fragments arranged by size: 852 322 88 42 35 11 9 BseYI C'CCAG_C Cuts at: 0 30 264 414 979 1359 Size: 30 234 150 565 380 Fragments arranged by size: 565 380 234 150 30 BsiHKAI G_wGCw'C Cuts at: 0 548 993 1061 1359 Size: 548 445 68 298 Fragments arranged by size: 548 445 298 68 Bsp1286I G_dGCh'C Cuts at: 0 548 993 1061 1359 Size: 548 445 68 298 Fragments arranged by size: 548 445 298 68 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 299 1359 Size: 299 1060 BsrBI CCG'CTC Cuts at: 0 15 1359 Size: 15 1344 BssSI C'ACGA_G Cuts at: 0 1039 1359 Size: 1039 320 BstAPI GCAn_nnn'nTGC Cuts at: 0 291 1359 Size: 291 1068 BstYI r'GATC_y Cuts at: 0 353 932 1049 1188 1244 1359 Size: 353 579 117 139 56 115 Fragments arranged by size: 579 353 139 117 115 56 BtgI C'CryG_G Cuts at: 0 275 527 1359 Size: 275 252 832 Fragments arranged by size: 832 275 252 DraIII CAC_nnn'GTG Cuts at: 0 528 1359 Size: 528 831 EaeI y'GGCC_r Cuts at: 0 272 332 1359 Size: 272 60 1027 Fragments arranged by size: 1027 272 60 EarI CTCTTCn'nnn_ Cuts at: 0 616 1359 Size: 616 743 EcoICRI GAG'CTC Cuts at: 0 546 1059 1359 Size: 546 513 300 Fragments arranged by size: 546 513 300 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 680 1147 1159 1359 Size: 403 277 467 12 200 Fragments arranged by size: 467 403 277 200 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 1162 1359 Size: 1162 197 EcoO109I rG'GnC_Cy Cuts at: 0 27 288 444 584 1359 Size: 27 261 156 140 775 Fragments arranged by size: 775 261 156 140 27 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 177 1288 1359 Size: 177 1111 71 Fragments arranged by size: 1111 177 71 HaeII r_GCGC'y Cuts at: 0 172 1359 Size: 172 1187 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 76 108 757 789 1267 1289 1299 1321 Size: 76 32 649 32 478 22 10 22 Cuts at: 1321 1359 Size: 38 Fragments arranged by size: 649 478 76 38 32 32 22 22 10 KasI G'GCGC_C Cuts at: 0 168 1359 Size: 168 1191 MfeI C'AATT_G Cuts at: 0 1333 1359 Size: 1333 26 MscI TGG'CCA Cuts at: 0 274 334 1359 Size: 274 60 1025 Fragments arranged by size: 1025 274 60 MspA1I CmG'CkG Cuts at: 0 264 315 350 436 609 729 837 1359 Size: 264 51 35 86 173 120 108 522 Fragments arranged by size: 522 264 173 120 108 86 51 35 NarI GG'CG_CC Cuts at: 0 169 1359 Size: 169 1190 NruI TCG'CGA Cuts at: 0 125 1359 Size: 125 1234 NspI r_CATG'y Cuts at: 0 93 414 818 898 1359 Size: 93 321 404 80 461 Fragments arranged by size: 461 404 321 93 80 PflMI CCAn_nnn'nTGG Cuts at: 0 204 528 1359 Size: 204 324 831 Fragments arranged by size: 831 324 204 PfoI T'CCnGG_A Cuts at: 0 923 1359 Size: 923 436 PmlI CAC'GTG Cuts at: 0 682 1359 Size: 682 677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 734 766 1359 Size: 734 32 593 Fragments arranged by size: 734 593 32 PpuMI rG'GwC_Cy Cuts at: 0 288 444 584 1359 Size: 288 156 140 775 Fragments arranged by size: 775 288 156 140 PspXI vC'TCGA_Gb Cuts at: 0 637 1359 Size: 637 722 PstI C_TGCA'G Cuts at: 0 287 314 608 614 1359 Size: 287 27 294 6 745 Fragments arranged by size: 745 294 287 27 6 PvuII CAG'CTG Cuts at: 0 350 436 609 729 837 1359 Size: 350 86 173 120 108 522 Fragments arranged by size: 522 350 173 120 108 86 SacI G_AGCT'C Cuts at: 0 548 1061 1359 Size: 548 513 298 Fragments arranged by size: 548 513 298 SfcI C'TryA_G Cuts at: 0 283 310 604 610 1359 Size: 283 27 294 6 749 Fragments arranged by size: 749 294 283 27 6 SfoI GGC'GCC Cuts at: 0 170 1359 Size: 170 1189 SmlI C'TyrA_G Cuts at: 0 391 637 853 1273 1359 Size: 391 246 216 420 86 Fragments arranged by size: 420 391 246 216 86 SphI G_CATG'C Cuts at: 0 818 898 1359 Size: 818 80 461 Fragments arranged by size: 818 461 80 StuI AGG'CCT Cuts at: 0 238 889 1359 Size: 238 651 470 Fragments arranged by size: 651 470 238 StyI C'CwwG_G Cuts at: 0 686 1240 1359 Size: 686 554 119 Fragments arranged by size: 686 554 119 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1084 1359 Size: 1084 275 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1182 1359 Size: 1182 177 XhoI C'TCGA_G Cuts at: 0 637 1359 Size: 637 722 XmnI GAAnn'nnTTC Cuts at: 0 746 1359 Size: 746 613 Enzymes that do cut: AccI AcuI AlwNI AvaI AvrII BanI BanII BbeI BbsI BbvCI BciVI BglI BlpI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI BssSI BstAPI BstYI BtgI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MfeI MscI MspA1I NarI NruI NspI PflMI PfoI PmlI PpiI PpuMI PspXI PstI PvuII SacI SfcI SfoI SmlI SphI StuI StyI TaqII XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI BaeI BamHI BcgI BclI BfrBI BglII Bme1580I BmgBI BmtI BplI BsaBI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MluI MmeI MslI NaeI NcoI NdeI NgoMIV NheI NotI NsiI PacI PasI PciI PmeI PshAI PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I XbaI XmaI ZraI