Restriction map for
pFN21AB7991
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI HphI KpnI Ksp632I
MaeI MamI MboII MfeI MluI
MseI MslI MunI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 109 GTCTAC GT^[AC][GT]AC
AccIII 187 TCCGGA T^CCGGA
AfaI 444 GTAC GT^AC
AscI 449 GGCGCGCC GG^CGCGCC
BamHI 941 GGATCC G^GATCC
BbvCI 769 CCTCAGC CCTCAGC(-5/-2)
BsaAI 112 TACGTG [CT]AC^GT[GA]
BseAI 187 TCCGGA T^CCGGA
BsgI 565 CTGCAC GTGCAG(16/14)
BsmBI 1232 CGTCTC CGTCTC(1/5)
BspEI 187 TCCGGA T^CCGGA
BssHII 450 GCGCGC G^CGCGC
BstYI 941 GGATCC [GA]^GATC[CT]
DpnII 942 GATC ^GATC
DraIII 837 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
HincII 652 GTCAAC GT[CT]^[GA]AC
HindII 652 GTCAAC GT[CT]^[GA]AC
HinfI 1273 GACTC G^A[ACGT]TC
MboI 942 GATC ^GATC
MflI 941 GGATCC [GA]^GATC[CT]
MroI 187 TCCGGA T^CCGGA
NdeII 942 GATC ^GATC
NotI 352 GCGGCCGC GC^GGCCGC
PflMI 836 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1273 GACTC GAGTC(4/5)
RsaI 444 GTAC GT^AC
Sau3AI 942 GATC ^GATC
SfcI 393 CTACAG C^T[GA][CT]AG
SgrAI 305 CGCCGGTG C[GA]^CCGG[CT]G
StuI 916 AGGCCT AGG^CCT
Van91I 836 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 338 CCACCGGGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 941 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 942 GATCC GGATC(4/5)
941 GGATC
ApaI 235 GGGCCC GGGCC^C
1177 GGGCCC
BalI 14 TGGCCA TGG^CCA
1170 TGGCCA
BsaWI 187 TCCGGA [AT]^CCGG[AT]
211 TCCGGT
BseRI 135 CTCCTC GAGGAG(10/8)
1075 GAGGAG
BsmAI 1233 GTCTC GTCTC(1/5)
1248 GTCTC
BsrI 106 CCAGT ACTGG(1/-1)
1245 CCAGT
BstF5I 1185 CATCC GGATG(2/0)
262 GGATG
DrdI 336 GACCACCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
553 GACAGCCCGGTC
EcoT14I 17 CCATGG C^C[AT][AT]GG
1173 CCATGG
FokI 1185 CATCC GGATG(9/13)
262 GGATG
KspI 124 CCGCGG CCGC^GG
371 CCGCGG
MaeIII 701 GTCAC ^GT[ACGT]AC
1304 GTCAC
MluNI 14 TGGCCA TGG^CCA
1170 TGGCCA
MscI 14 TGGCCA TGG^CCA
1170 TGGCCA
NcoI 17 CCATGG C^CATGG
1173 CCATGG
SacII 124 CCGCGG CCGC^GG
371 CCGCGG
SfaNI 1080 GATGC GCATC(5/9)
746 GCATC
SfiI 424 GGCCTGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
585 GGCCAACCCGGCC
StyI 17 CCATGG C^C[AT][AT]GG
1173 CCATGG
TaqI 551 TCGA T^CGA
614 TCGA
Tsp45I 701 GTCAC ^GT[GC]AC
1304 GTCAC
TthHB8I 551 TCGA T^CGA
614 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrBI BsrDI BsrFI
BssKI BstAPI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI KasI MaeII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NgoMIV
NlaIII NlaIV PpuMI Sau96I ScrFI
SfoI SmaI TseI TspRI XmaCI
XmaI