(Circular) (Six-base) MAPSORT of: pFN21AB7991.seq Check: 4392 from: 1 to: 6031 pFN21AB7991 6031 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3841 278 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2115 3374 1937 Size: 178 1259 4594 Fragments arranged by size: 4594 1259 178 Acc65I G'GTAC_C Cuts at: 3352 3352 Size: 6031 AclI AA'CG_TT Cuts at: 1563 4158 4531 1563 Size: 2595 373 3063 Fragments arranged by size: 3063 2595 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2991 3233 4094 5478 5746 1632 Size: 1359 242 861 1384 268 1917 Fragments arranged by size: 1917 1384 1359 861 268 242 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5834 AflIII A'CryG_T Cuts at: 1203 4930 5907 1203 Size: 3727 977 1327 Fragments arranged by size: 3727 1327 977 AgeI A'CCGG_T Cuts at: 4862 4862 Size: 6031 AhdI GACnn_n'nnGTC Cuts at: 4760 4760 Size: 6031 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6031 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6031 AlwNI CAG_nnn'CTG Cuts at: 3774 5346 3774 Size: 1572 4459 ApaI G_GGCC'C Cuts at: 2244 3186 2244 Size: 942 5089 ApaLI G'TGCA_C Cuts at: 4088 5244 4088 Size: 1156 4875 ApoI r'AATT_y Cuts at: 1493 3340 3566 3718 5827 1493 Size: 1847 226 152 2109 1697 Fragments arranged by size: 2109 1847 1697 226 152 AscI GG'CGCG_CC Cuts at: 2455 2455 Size: 6031 AseI AT'TA_AT Cuts at: 160 1032 4585 160 Size: 872 3553 1606 Fragments arranged by size: 3553 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 AvaI C'yCGr_G Cuts at: 1870 1943 2203 2243 2263 2335 2382 3356 1870 Size: 73 260 40 20 72 47 974 4545 Fragments arranged by size: 4545 974 260 73 72 47 40 20 BamHI G'GATC_C Cuts at: 2946 3361 3728 2946 Size: 415 367 5249 Fragments arranged by size: 5249 415 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2075 2096 2134 2198 Size: 325 217 627 264 24 21 38 64 Cuts at: 2198 2850 3352 4807 618 Size: 652 502 1455 1842 Fragments arranged by size: 1842 1455 652 627 502 325 264 217 64 38 24 21 BanII G_rGCy'C Cuts at: 1391 2186 2244 2474 3186 3350 1391 Size: 795 58 230 712 164 4072 Fragments arranged by size: 4072 795 712 230 164 58 BbeI G_GCGC'C Cuts at: 2079 2100 2202 2854 2079 Size: 21 102 652 5256 Fragments arranged by size: 5256 652 102 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5060 BbvCI CC'TCA_GC Cuts at: 2775 2775 Size: 6031 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4222 4256 1903 Size: 34 2285 34 3678 Fragments arranged by size: 3678 2285 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3923 5133 3923 Size: 1210 4821 BclI T'GATC_A Cuts at: 1548 1548 Size: 6031 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2060 2436 2583 Size: 107 122 71 631 605 388 376 147 Cuts at: 2583 2597 2805 4642 136 Size: 14 208 1837 1525 Fragments arranged by size: 1837 1525 631 605 388 376 208 147 122 107 71 14 BglII A'GATC_T Cuts at: 6026 6026 Size: 6031 BlpI GC'TnA_GC Cuts at: 3448 3448 Size: 6031 Bme1580I G_kGCm'C Cuts at: 1790 2124 2139 2244 3132 3186 4092 5248 1790 Size: 334 15 105 888 54 906 1156 2573 Fragments arranged by size: 2573 1156 906 888 334 105 54 15 BmgBI CAC'GTC Cuts at: 1318 2509 1318 Size: 1191 4840 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3762 4720 442 Size: 416 53 83 399 24 2345 958 1753 Fragments arranged by size: 2345 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6031 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2825 3042 2825 Size: 217 5814 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2464 2707 3037 4691 1239 Size: 911 314 243 330 1654 2579 Fragments arranged by size: 2579 1654 911 330 314 243 Bpu10I CC'TnA_GC Cuts at: 2775 2775 Size: 6031 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4156 5021 5319 4156 Size: 865 298 4868 Fragments arranged by size: 4868 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4694 882 Size: 617 3195 2219 Fragments arranged by size: 3195 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2119 3822 493 Size: 711 915 1703 2702 Fragments arranged by size: 2702 1703 915 711 BsaBI GATnn'nnATC Cuts at: 6025 6025 Size: 6031 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2076 2097 2199 2851 Size: 53 83 186 936 543 21 102 652 Cuts at: 2851 3838 4220 4855 275 Size: 987 382 635 1451 Fragments arranged by size: 1451 987 936 652 635 543 382 186 102 83 53 21 BsaWI w'CCGG_w Cuts at: 2192 2216 4464 4862 5136 5283 5694 2192 Size: 24 2248 398 274 147 411 2529 Fragments arranged by size: 2529 2248 411 398 274 147 24 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3122 3152 1178 Size: 30 165 30 1719 30 4057 Fragments arranged by size: 4057 1719 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2131 3095 1186 Size: 945 964 4122 Fragments arranged by size: 4122 964 945 BseYI C'CCAG_C Cuts at: 2149 2250 5234 5730 2149 Size: 101 2984 496 2450 Fragments arranged by size: 2984 2450 496 101 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2555 1163 Size: 573 819 4639 Fragments arranged by size: 4639 819 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2361 2587 2601 2762 3481 4242 Size: 275 186 360 226 14 161 719 761 Cuts at: 4242 5270 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 719 360 275 226 186 161 14 BsiHKAI G_wGCw'C Cuts at: 4092 4177 5248 4092 Size: 85 1071 4875 Fragments arranged by size: 4875 1071 85 BsmI GAATG_Cn' Cuts at: 3542 3635 3542 Size: 93 5938 BsmBI CGTCTCn'nnnn_ Cuts at: 3243 3243 Size: 6031 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2139 2186 2244 2474 3132 3186 Size: 399 334 15 47 58 230 658 54 Cuts at: 3186 3350 4092 4177 5248 1391 Size: 164 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 658 399 334 230 164 85 58 54 47 15 BspEI T'CCGG_A Cuts at: 2192 5694 2192 Size: 3502 2529 BspHI T'CATG_A Cuts at: 3920 5650 3920 Size: 1730 4301 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3386 844 Size: 2542 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2417 2683 2764 3918 1394 Size: 429 594 266 81 1154 3507 Fragments arranged by size: 3507 1154 594 429 266 81 BsrDI GCAATG_nn' Cuts at: 65 1248 2863 2887 2937 4526 4700 65 Size: 1183 1615 24 50 1589 174 1396 Fragments arranged by size: 1615 1589 1396 1183 174 50 24 BsrFI r'CCGG_y Cuts at: 1700 1810 2311 2459 2546 2757 2781 4675 4862 Size: 110 501 148 87 211 24 1894 187 Cuts at: 4862 1700 Size: 2869 Fragments arranged by size: 2869 1894 501 211 187 148 110 87 24 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6031 BssHII G'CGCG_C Cuts at: 1925 2455 1925 Size: 530 5501 BssSI C'ACGA_G Cuts at: 4091 5103 4091 Size: 1012 5019 BstAPI GCAn_nnn'nTGC Cuts at: 794 2578 2724 2886 794 Size: 1784 146 162 3939 Fragments arranged by size: 3939 1784 162 146 BstBI TT'CG_AA Cuts at: 4835 4835 Size: 6031 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6031 BstYI r'GATC_y Cuts at: 1972 2946 3361 3728 4113 4130 5582 5668 5680 Size: 974 415 367 385 17 1452 86 12 Cuts at: 5680 6026 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 974 415 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2035 2129 2376 2908 3178 513 Size: 552 957 13 94 247 532 270 3366 Fragments arranged by size: 3366 957 552 532 270 247 94 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3182 487 Size: 683 56 1956 3336 Fragments arranged by size: 3336 1956 683 56 BtsI GCAGTG_nn' Cuts at: 2410 3019 3324 3547 4341 4361 2410 Size: 609 305 223 794 20 4080 Fragments arranged by size: 4080 794 609 305 223 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6031 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5996 DraI TTT'AAA Cuts at: 3335 3335 Size: 6031 DraIII CAC_nnn'GTG Cuts at: 2847 2847 Size: 6031 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2347 2564 5038 809 Size: 507 1031 217 2474 1802 Fragments arranged by size: 2474 1802 1031 507 217 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2025 2358 Size: 54 1425 50 275 18 189 6 333 Cuts at: 2358 2502 2584 2598 2759 3175 3300 3478 4367 Size: 144 82 14 161 416 125 178 889 Cuts at: 4367 5996 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 416 333 275 189 178 161 144 125 82 54 50 43 18 14 6 EagI C'GGCC_G Cuts at: 1537 1812 2358 2584 2598 2759 3478 1537 Size: 275 546 226 14 161 719 4090 Fragments arranged by size: 4090 719 546 275 226 161 14 EarI CTCTTCn'nnn_ Cuts at: 1353 3961 1353 Size: 2608 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2187 2427 2533 2779 4618 4992 Size: 1064 138 598 240 106 246 1839 374 Cuts at: 4992 5138 387 Size: 146 1280 Fragments arranged by size: 1839 1280 1064 598 374 246 240 146 138 106 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2464 2707 2991 3037 3233 4094 Size: 393 518 314 243 284 46 196 861 Cuts at: 4094 4691 5478 5746 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 861 787 597 518 393 314 284 268 243 196 46 EcoO109I rG'GnC_Cy Cuts at: 2240 2247 2349 2914 3183 2240 Size: 7 102 565 269 5088 Fragments arranged by size: 5088 565 269 102 7 EcoRI G'AATT_C Cuts at: 3340 3718 3340 Size: 378 5653 FspI TGC'GCA Cuts at: 4537 4537 Size: 6031 HaeII r_GCGC'y Cuts at: 1678 2079 2100 2202 2782 2854 5178 1678 Size: 401 21 102 580 72 2324 2531 Fragments arranged by size: 2531 2324 580 401 102 72 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2621 2640 2653 2672 4672 Size: 32 135 32 1049 19 13 19 2000 Cuts at: 4672 4704 4746 4778 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2000 1049 135 42 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 2659 3375 3621 669 Size: 988 281 721 716 246 3079 Fragments arranged by size: 3079 988 721 716 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6031 HpaI GTT'AAC Cuts at: 3621 3621 Size: 6031 KasI G'GCGC_C Cuts at: 2075 2096 2198 2850 2075 Size: 21 102 652 5256 Fragments arranged by size: 5256 652 102 21 KpnI G_GTAC'C Cuts at: 3356 3356 Size: 6031 MfeI C'AATT_G Cuts at: 3630 3630 Size: 6031 MluI A'CGCG_T Cuts at: 5907 5907 Size: 6031 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5145 5329 1305 Size: 3840 184 2007 Fragments arranged by size: 3840 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2021 3177 10 Size: 54 1425 343 189 1156 2864 Fragments arranged by size: 2864 1425 1156 343 189 54 MslI CAynn'nnrTG Cuts at: 518 1166 3989 4348 4507 518 Size: 648 2823 359 159 2042 Fragments arranged by size: 2823 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2131 2372 2378 3036 3447 4124 5272 5517 Size: 525 241 6 658 411 677 1148 245 Cuts at: 5517 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 658 525 411 245 241 6 NaeI GCC'GGC Cuts at: 2461 2548 2783 2461 Size: 87 235 5709 Fragments arranged by size: 5709 235 87 NarI GG'CG_CC Cuts at: 2076 2097 2199 2851 2076 Size: 21 102 652 5256 Fragments arranged by size: 5256 652 102 21 NcoI C'CATG_G Cuts at: 513 1065 2022 3178 513 Size: 552 957 1156 3366 Fragments arranged by size: 3366 1156 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6031 NgoMIV G'CCGG_C Cuts at: 2459 2546 2781 2459 Size: 87 235 5709 Fragments arranged by size: 5709 235 87 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6031 NotI GC'GGCC_GC Cuts at: 2358 3478 2358 Size: 1120 4911 NspI r_CATG'y Cuts at: 1132 1229 3389 4934 1132 Size: 97 2160 1545 2229 Fragments arranged by size: 2229 2160 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6031 PciI A'CATG_T Cuts at: 4930 4930 Size: 6031 PflMI CCAn_nnn'nTGG Cuts at: 1108 2847 1108 Size: 1739 4292 PmeI GTTT'AAAC Cuts at: 3335 3335 Size: 6031 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4100 4132 5613 5645 4100 Size: 32 1481 32 4486 Fragments arranged by size: 4486 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2247 2349 2914 2247 Size: 102 565 5364 Fragments arranged by size: 5364 565 102 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6031 PsiI TTA'TAA Cuts at: 3601 3601 Size: 6031 PspOMI G'GGCC_C Cuts at: 2240 3182 2240 Size: 942 5089 PstI C_TGCA'G Cuts at: 830 3383 830 Size: 2553 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6031 SacII CC_GC'GG Cuts at: 2132 2379 2132 Size: 247 5784 SalI G'TCGA_C Cuts at: 1936 3373 1936 Size: 1437 4594 SanDI GG'GwC_CC Cuts at: 2247 2349 2914 2247 Size: 102 565 5364 Fragments arranged by size: 5364 565 102 SbfI CC_TGCA'GG Cuts at: 3383 3383 Size: 6031 ScaI AGT'ACT Cuts at: 4279 5991 4279 Size: 1712 4319 SfcI C'TryA_G Cuts at: 826 1045 2398 3379 4514 5195 5386 826 Size: 219 1353 981 1135 681 191 1471 Fragments arranged by size: 1471 1353 1135 981 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2436 2597 2436 Size: 161 5870 SfoI GGC'GCC Cuts at: 2077 2098 2200 2852 2077 Size: 21 102 652 5256 Fragments arranged by size: 5256 652 102 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 SgrAI Cr'CCGG_yG Cuts at: 1700 2311 1700 Size: 611 5420 SmaI CCC'GGG Cuts at: 1872 2245 2265 2337 2384 3358 1872 Size: 373 20 72 47 974 4545 Fragments arranged by size: 4545 974 373 72 47 20 SmlI C'TyrA_G Cuts at: 820 1017 1943 4135 5036 5298 820 Size: 197 926 2192 901 262 1553 Fragments arranged by size: 2192 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3822 493 Size: 3329 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6031 SphI G_CATG'C Cuts at: 1132 3389 1132 Size: 2257 3774 SspI AAT'ATT Cuts at: 5 52 3955 5 Size: 47 3903 2081 Fragments arranged by size: 3903 2081 47 StuI AGG'CCT Cuts at: 1517 2923 1517 Size: 1406 4625 StyI C'CwwG_G Cuts at: 513 1065 2022 3178 3470 513 Size: 552 957 1156 292 3074 Fragments arranged by size: 3074 1156 957 552 292 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4058 4075 4228 4413 4858 325 Size: 1255 2478 17 153 185 445 1498 Fragments arranged by size: 2478 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4277 5989 96 Size: 274 80 33 51 1443 2300 1712 138 Fragments arranged by size: 2300 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3122 3154 5991 6023 3122 Size: 32 2837 32 3130 Fragments arranged by size: 3130 2837 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6031 XbaI T'CTAG_A Cuts at: 3367 3367 Size: 6031 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2350 2350 Size: 6031 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6031 XmaI C'CCGG_G Cuts at: 1870 2243 2263 2335 2382 3356 1870 Size: 373 20 72 47 974 4545 Fragments arranged by size: 4545 974 373 72 47 20 XmnI GAAnn'nnTTC Cuts at: 4160 4160 Size: 6031 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3839 276 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SapI SexAI SrfI SwaI