Restriction map for
pFN21AB7991
(Linear) (Six-base) MAPSORT of: KIBB7991.seq Check: 5236 from: 1 to: 1326
KIBB7991.orf
With 182 enzymes: SgfI *
November 19, 2010 15:54 ..
AccI GT'mk_AC
Cuts at: 0 110 1326
Size: 110 1216
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 986 1228 1326
Size: 986 242 98
Fragments arranged by size:
986 242 98
ApaI G_GGCC'C
Cuts at: 0 239 1181 1326
Size: 239 942 145
Fragments arranged by size:
942 239 145
AscI GG'CGCG_CC
Cuts at: 0 450 1326
Size: 450 876
AvaI C'yCGr_G
Cuts at: 0 198 238 258 330 377 1326
Size: 198 40 20 72 47 949
Fragments arranged by size:
949 198 72 47 40 20
BamHI G'GATC_C
Cuts at: 0 941 1326
Size: 941 385
BanI G'GyrC_C
Cuts at: 0 46 70 91 129 193 845 1326
Size: 46 24 21 38 64 652 481
Fragments arranged by size:
652 481 64 46 38 24 21
BanII G_rGCy'C
Cuts at: 0 181 239 469 1181 1326
Size: 181 58 230 712 145
Fragments arranged by size:
712 230 181 145 58
BbeI G_GCGC'C
Cuts at: 0 74 95 197 849 1326
Size: 74 21 102 652 477
Fragments arranged by size:
652 477 102 74 21
BbvCI CC'TCA_GC
Cuts at: 0 770 1326
Size: 770 556
BglI GCCn_nnn'nGGC
Cuts at: 0 55 431 578 592 800 1326
Size: 55 376 147 14 208 526
Fragments arranged by size:
526 376 208 147 55 14
Bme1580I G_kGCm'C
Cuts at: 0 119 134 239 1127 1181 1326
Size: 119 15 105 888 54 145
Fragments arranged by size:
888 145 119 105 54 15
BmgBI CAC'GTC
Cuts at: 0 504 1326
Size: 504 822
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 820 1037 1326
Size: 820 217 289
Fragments arranged by size:
820 289 217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 459 702 1032 1326
Size: 145 314 243 330 294
Fragments arranged by size:
330 314 294 243 145
Bpu10I CC'TnA_GC
Cuts at: 0 770 1326
Size: 770 556
BsaAI yAC'GTr
Cuts at: 0 114 1326
Size: 114 1212
BsaHI Gr'CG_yC
Cuts at: 0 71 92 194 846 1326
Size: 71 21 102 652 480
Fragments arranged by size:
652 480 102 71 21
BsaWI w'CCGG_w
Cuts at: 0 187 211 1326
Size: 187 24 1115
Fragments arranged by size:
1115 187 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1117 1147 1326
Size: 1117 30 179
Fragments arranged by size:
1117 179 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 126 1090 1326
Size: 126 964 236
Fragments arranged by size:
964 236 126
BseYI C'CCAG_C
Cuts at: 0 144 245 1326
Size: 144 101 1081
Fragments arranged by size:
1081 144 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 1326
Size: 550 776
BsiEI CG_ry'CG
Cuts at: 0 356 582 596 757 1326
Size: 356 226 14 161 569
Fragments arranged by size:
569 356 226 161 14
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1238 1326
Size: 1238 88
Bsp1286I G_dGCh'C
Cuts at: 0 119 134 181 239 469 1127 1181 1326
Size: 119 15 47 58 230 658 54 145
Fragments arranged by size:
658 230 145 119 58 54 47 15
BspEI T'CCGG_A
Cuts at: 0 187 1326
Size: 187 1139
BsrBI CCG'CTC
Cuts at: 0 412 678 759 1326
Size: 412 266 81 567
Fragments arranged by size:
567 412 266 81
BsrDI GCAATG_nn'
Cuts at: 0 858 882 932 1326
Size: 858 24 50 394
Fragments arranged by size:
858 394 50 24
BsrFI r'CCGG_y
Cuts at: 0 306 454 541 752 776 1326
Size: 306 148 87 211 24 550
Fragments arranged by size:
550 306 211 148 87 24
BssHII G'CGCG_C
Cuts at: 0 450 1326
Size: 450 876
BstAPI GCAn_nnn'nTGC
Cuts at: 0 573 719 881 1326
Size: 573 146 162 445
Fragments arranged by size:
573 445 162 146
BstYI r'GATC_y
Cuts at: 0 941 1326
Size: 941 385
BtgI C'CryG_G
Cuts at: 0 17 30 124 371 903 1173 1326
Size: 17 13 94 247 532 270 153
Fragments arranged by size:
532 270 247 153 94 17 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1177 1326
Size: 1177 149
BtsI GCAGTG_nn'
Cuts at: 0 405 1014 1319 1326
Size: 405 609 305 7
Fragments arranged by size:
609 405 305 7
DraIII CAC_nnn'GTG
Cuts at: 0 842 1326
Size: 842 484
DrdI GACnn_nn'nnGTC
Cuts at: 0 342 559 1326
Size: 342 217 767
Fragments arranged by size:
767 342 217
EaeI y'GGCC_r
Cuts at: 0 14 20 353 497 579 593 754 1170
Size: 14 6 333 144 82 14 161 416
Cuts at: 1170 1295 1326
Size: 125 31
Fragments arranged by size:
416 333 161 144 125 82 31 14
14 6
EagI C'GGCC_G
Cuts at: 0 353 579 593 754 1326
Size: 353 226 14 161 572
Fragments arranged by size:
572 353 226 161 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 182 422 528 774 1326
Size: 182 240 106 246 552
Fragments arranged by size:
552 246 240 182 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 459 702 986 1032 1228 1326
Size: 145 314 243 284 46 196 98
Fragments arranged by size:
314 284 243 196 145 98 46
EcoO109I rG'GnC_Cy
Cuts at: 0 235 242 344 909 1178 1326
Size: 235 7 102 565 269 148
Fragments arranged by size:
565 269 235 148 102 7
HaeII r_GCGC'y
Cuts at: 0 74 95 197 777 849 1326
Size: 74 21 102 580 72 477
Fragments arranged by size:
580 477 102 74 72 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 616 635 648 667 1326
Size: 616 19 13 19 659
Fragments arranged by size:
659 616 19 19 13
HincII GTy'rAC
Cuts at: 0 654 1326
Size: 654 672
KasI G'GCGC_C
Cuts at: 0 70 91 193 845 1326
Size: 70 21 102 652 481
Fragments arranged by size:
652 481 102 70 21
MscI TGG'CCA
Cuts at: 0 16 1172 1326
Size: 16 1156 154
Fragments arranged by size:
1156 154 16
MspA1I CmG'CkG
Cuts at: 0 126 367 373 1031 1326
Size: 126 241 6 658 295
Fragments arranged by size:
658 295 241 126 6
NaeI GCC'GGC
Cuts at: 0 456 543 778 1326
Size: 456 87 235 548
Fragments arranged by size:
548 456 235 87
NarI GG'CG_CC
Cuts at: 0 71 92 194 846 1326
Size: 71 21 102 652 480
Fragments arranged by size:
652 480 102 71 21
NcoI C'CATG_G
Cuts at: 0 17 1173 1326
Size: 17 1156 153
Fragments arranged by size:
1156 153 17
NgoMIV G'CCGG_C
Cuts at: 0 454 541 776 1326
Size: 454 87 235 550
Fragments arranged by size:
550 454 235 87
NotI GC'GGCC_GC
Cuts at: 0 353 1326
Size: 353 973
PflMI CCAn_nnn'nTGG
Cuts at: 0 842 1326
Size: 842 484
PpuMI rG'GwC_Cy
Cuts at: 0 242 344 909 1326
Size: 242 102 565 417
Fragments arranged by size:
565 417 242 102
PspOMI G'GGCC_C
Cuts at: 0 235 1177 1326
Size: 235 942 149
Fragments arranged by size:
942 235 149
SacII CC_GC'GG
Cuts at: 0 127 374 1326
Size: 127 247 952
Fragments arranged by size:
952 247 127
SanDI GG'GwC_CC
Cuts at: 0 242 344 909 1326
Size: 242 102 565 417
Fragments arranged by size:
565 417 242 102
SfcI C'TryA_G
Cuts at: 0 393 1326
Size: 393 933
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 431 592 1326
Size: 431 161 734
Fragments arranged by size:
734 431 161
SfoI GGC'GCC
Cuts at: 0 72 93 195 847 1326
Size: 72 21 102 652 479
Fragments arranged by size:
652 479 102 72 21
SgrAI Cr'CCGG_yG
Cuts at: 0 306 1326
Size: 306 1020
SmaI CCC'GGG
Cuts at: 0 240 260 332 379 1326
Size: 240 20 72 47 947
Fragments arranged by size:
947 240 72 47 20
StuI AGG'CCT
Cuts at: 0 918 1326
Size: 918 408
StyI C'CwwG_G
Cuts at: 0 17 1173 1326
Size: 17 1156 153
Fragments arranged by size:
1156 153 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1117 1149 1326
Size: 1117 32 177
Fragments arranged by size:
1117 177 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 345 1326
Size: 345 981
XmaI C'CCGG_G
Cuts at: 0 238 258 330 377 1326
Size: 238 20 72 47 949
Fragments arranged by size:
949 238 72 47 20
Enzymes that do cut:
AccI AcuI ApaI AscI AvaI BamHI BanI BanII
BbeI BbvCI BglI Bme1580I BmgBI BplI BpmI Bpu10I
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI BssHII BstAPI
BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EagI
EciI Eco57MI EcoO109I HaeII Hin4I HincII KasI MscI
MspA1I NaeI NarI NcoI NgoMIV NotI PflMI PpuMI
PspOMI SacII SanDI SfcI SfiI SfoI SgrAI SmaI
StuI StyI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvrII BaeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BpuEI BsaI BsaBI BsiHKAI
BsiWI BsmI BspHI BspMI BsrGI BssSI BstBI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraI EarI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MmeI MslI NdeI NheI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SgfI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI Tth111I XbaI XhoI XmnI ZraI