(Linear) (Six-base) MAPSORT of: KIEE3220.seq Check: 5744 from: 1 to: 1488 KIEE3220.orf With 182 enzymes: SgfI * July 3, 2012 17:28 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 457 825 875 1031 1488 Size: 457 368 50 156 457 Fragments arranged by size: 457 457 368 156 50 AflIII A'CryG_T Cuts at: 0 179 893 1488 Size: 179 714 595 Fragments arranged by size: 714 595 179 AlwNI CAG_nnn'CTG Cuts at: 0 237 848 1488 Size: 237 611 640 Fragments arranged by size: 640 611 237 ApoI r'AATT_y Cuts at: 0 669 1488 Size: 669 819 BbsI GAAGACnn'nnnn_ Cuts at: 0 602 1488 Size: 602 886 BciVI GTATCCnnnnn_n' Cuts at: 0 669 1488 Size: 669 819 BfrBI ATG'CAT Cuts at: 0 1345 1488 Size: 1345 143 BmgBI CAC'GTC Cuts at: 0 982 1488 Size: 982 506 BmrI ACTGGGnnnn_n' Cuts at: 0 1140 1488 Size: 1140 348 BmtI G_CTAG'C Cuts at: 0 589 1488 Size: 589 899 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1167 1488 Size: 1167 321 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 268 971 1488 Size: 153 115 703 517 Fragments arranged by size: 703 517 153 115 Bpu10I CC'TnA_GC Cuts at: 0 803 1437 1488 Size: 803 634 51 Fragments arranged by size: 803 634 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 1161 1435 1488 Size: 310 851 274 53 Fragments arranged by size: 851 310 274 53 BsaAI yAC'GTr Cuts at: 0 180 1107 1488 Size: 180 927 381 Fragments arranged by size: 927 381 180 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 968 1488 Size: 968 520 BseYI C'CCAG_C Cuts at: 0 1062 1488 Size: 1062 426 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 250 1488 Size: 214 36 1238 Fragments arranged by size: 1238 214 36 BsmI GAATG_Cn' Cuts at: 0 348 1488 Size: 348 1140 BsrBI CCG'CTC Cuts at: 0 161 210 1488 Size: 161 49 1278 Fragments arranged by size: 1278 161 49 BsrFI r'CCGG_y Cuts at: 0 1228 1488 Size: 1228 260 BssSI C'ACGA_G Cuts at: 0 459 1488 Size: 459 1029 BstAPI GCAn_nnn'nTGC Cuts at: 0 237 1488 Size: 237 1251 BstEII G'GTnAC_C Cuts at: 0 1039 1488 Size: 1039 449 BtgI C'CryG_G Cuts at: 0 710 1488 Size: 710 778 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 447 482 1488 Size: 447 35 1006 Fragments arranged by size: 1006 447 35 DraIII CAC_nnn'GTG Cuts at: 0 275 1488 Size: 275 1213 DrdI GACnn_nn'nnGTC Cuts at: 0 783 1488 Size: 783 705 EaeI y'GGCC_r Cuts at: 0 44 1488 Size: 44 1444 EarI CTCTTCn'nnn_ Cuts at: 0 64 168 626 914 1488 Size: 64 104 458 288 574 Fragments arranged by size: 574 458 288 104 64 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 268 457 825 875 971 1031 1488 Size: 153 115 189 368 50 96 60 457 Fragments arranged by size: 457 368 189 153 115 96 60 50 EcoO109I rG'GnC_Cy Cuts at: 0 116 807 1488 Size: 116 691 681 Fragments arranged by size: 691 681 116 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 18 50 1488 Size: 18 32 1438 Fragments arranged by size: 1438 32 18 HincII GTy'rAC Cuts at: 0 425 899 1488 Size: 425 474 589 Fragments arranged by size: 589 474 425 HindIII A'AGCT_T Cuts at: 0 286 1488 Size: 286 1202 HpaI GTT'AAC Cuts at: 0 425 899 1488 Size: 425 474 589 Fragments arranged by size: 589 474 425 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 636 1382 1488 Size: 636 746 106 Fragments arranged by size: 746 636 106 MslI CAynn'nnrTG Cuts at: 0 1462 1488 Size: 1462 26 MspA1I CmG'CkG Cuts at: 0 406 1488 Size: 406 1082 NaeI GCC'GGC Cuts at: 0 1230 1488 Size: 1230 258 NgoMIV G'CCGG_C Cuts at: 0 1228 1488 Size: 1228 260 NheI G'CTAG_C Cuts at: 0 585 1488 Size: 585 903 NsiI A_TGCA'T Cuts at: 0 1347 1488 Size: 1347 141 NspI r_CATG'y Cuts at: 0 1349 1488 Size: 1349 139 PfoI T'CCnGG_A Cuts at: 0 302 1488 Size: 302 1186 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 461 476 493 508 1488 Size: 461 15 17 15 980 Fragments arranged by size: 980 461 17 15 15 PpuMI rG'GwC_Cy Cuts at: 0 116 807 1488 Size: 116 691 681 Fragments arranged by size: 691 681 116 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1357 1389 1488 Size: 1357 32 99 Fragments arranged by size: 1357 99 32 PstI C_TGCA'G Cuts at: 0 124 1488 Size: 124 1364 SapI GCTCTTCn'nnn_ Cuts at: 0 64 168 1488 Size: 64 104 1320 Fragments arranged by size: 1320 104 64 SfcI C'TryA_G Cuts at: 0 120 616 646 914 1488 Size: 120 496 30 268 574 Fragments arranged by size: 574 496 268 120 30 SmlI C'TyrA_G Cuts at: 0 289 1176 1450 1488 Size: 289 887 274 38 Fragments arranged by size: 887 289 274 38 SspI AAT'ATT Cuts at: 0 1057 1488 Size: 1057 431 StuI AGG'CCT Cuts at: 0 931 1488 Size: 931 557 TatI w'GTAC_w Cuts at: 0 348 1373 1488 Size: 348 1025 115 Fragments arranged by size: 1025 348 115 XmnI GAAnn'nnTTC Cuts at: 0 565 1488 Size: 565 923 Enzymes that do cut: AcuI AflIII AlwNI ApoI BbsI BciVI BfrBI BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BseRI BseYI BsgI BsmI BsrBI BsrFI BssSI BstAPI BstEII BtgI CspCI DraIII DrdI EaeI EarI Eco57MI EcoO109I Hin4I HincII HindIII HpaI MmeI MslI MspA1I NaeI NgoMIV NheI NsiI NspI PfoI PpiI PpuMI PsrI PstI SapI SfcI SmlI SspI StuI TatI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BsaI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BssHII BstBI BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI DraI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII KasI KpnI MfeI MluI MscI NarI NcoI NdeI NotI NruI PacI PasI PciI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI