(Circular) (Six-base) MAPSORT of: pFN21AE6676.seq Check: 5871 from: 1 to: 5563 pFN21AE6676 5563 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3373 278 Size: 53 83 186 936 1837 2468 Fragments arranged by size: 2468 1837 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2906 1937 Size: 969 4594 Acc65I G'GTAC_C Cuts at: 2884 2884 Size: 5563 AclI AA'CG_TT Cuts at: 1563 3690 4063 1563 Size: 2127 373 3063 Fragments arranged by size: 3063 2127 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3626 5010 5278 1632 Size: 1994 1384 268 1917 Fragments arranged by size: 1994 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5366 AflIII A'CryG_T Cuts at: 1203 2210 2601 2634 4462 5439 1203 Size: 1007 391 33 1828 977 1327 Fragments arranged by size: 1828 1327 1007 977 391 33 AgeI A'CCGG_T Cuts at: 4394 4394 Size: 5563 AhdI GACnn_n'nnGTC Cuts at: 4292 4292 Size: 5563 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5563 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5563 AlwNI CAG_nnn'CTG Cuts at: 2590 2845 3306 4878 2590 Size: 255 461 1572 3275 Fragments arranged by size: 3275 1572 461 255 ApaLI G'TGCA_C Cuts at: 3620 4776 3620 Size: 1156 4407 ApoI r'AATT_y Cuts at: 1493 2872 3098 3250 5359 1493 Size: 1379 226 152 2109 1697 Fragments arranged by size: 2109 1697 1379 226 152 AseI AT'TA_AT Cuts at: 160 1032 2739 4117 160 Size: 872 1707 1378 1606 Fragments arranged by size: 1707 1606 1378 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5563 AvaI C'yCGr_G Cuts at: 1870 1943 2888 1870 Size: 73 945 4545 Fragments arranged by size: 4545 945 73 BamHI G'GATC_C Cuts at: 2070 2893 3260 2070 Size: 823 367 4373 Fragments arranged by size: 4373 823 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2884 4339 618 Size: 325 217 627 1097 1455 1842 Fragments arranged by size: 1842 1455 1097 627 325 217 BanII G_rGCy'C Cuts at: 1391 2257 2882 1391 Size: 866 625 4072 Fragments arranged by size: 4072 866 625 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2095 2182 2768 928 Size: 971 196 87 586 3723 Fragments arranged by size: 3723 971 586 196 87 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3754 3788 1903 Size: 34 1817 34 3678 Fragments arranged by size: 3678 1817 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3455 4665 3455 Size: 1210 4353 BclI T'GATC_A Cuts at: 1548 1548 Size: 5563 BfrBI ATG'CAT Cuts at: 2060 2060 Size: 5563 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2288 4174 136 Size: 107 122 71 631 605 616 1886 1525 Fragments arranged by size: 1886 1525 631 616 605 122 107 71 BglII A'GATC_T Cuts at: 5558 5558 Size: 5563 BlpI GC'TnA_GC Cuts at: 2980 2980 Size: 5563 Bme1580I G_kGCm'C Cuts at: 1790 2030 3624 4780 1790 Size: 240 1594 1156 2573 Fragments arranged by size: 2573 1594 1156 240 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5563 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2668 3294 4252 Size: 416 53 83 399 24 1251 626 958 Cuts at: 4252 442 Size: 1753 Fragments arranged by size: 1753 1251 958 626 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5563 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2522 4223 1239 Size: 1283 1701 2579 Fragments arranged by size: 2579 1701 1283 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3688 4553 4851 3688 Size: 865 298 4400 Fragments arranged by size: 4400 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4226 882 Size: 617 2727 2219 Fragments arranged by size: 2727 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3354 493 Size: 711 2150 2702 Fragments arranged by size: 2702 2150 711 BsaBI GATnn'nnATC Cuts at: 5557 5557 Size: 5563 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2115 3370 3752 4387 Size: 53 83 186 936 582 1255 382 635 Cuts at: 4387 275 Size: 1451 Fragments arranged by size: 1451 1255 936 635 582 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2388 2782 3996 4394 4668 4815 5226 2388 Size: 394 1214 398 274 147 411 2725 Fragments arranged by size: 2725 1214 411 398 394 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5338 Fragments arranged by size: 5338 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5563 BseYI C'CCAG_C Cuts at: 2260 4766 5262 2260 Size: 2506 496 2561 Fragments arranged by size: 2561 2506 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2235 1163 Size: 573 499 4491 Fragments arranged by size: 4491 573 499 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3013 3774 4802 1540 Size: 275 186 1012 761 1028 2301 Fragments arranged by size: 2301 1028 1012 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2237 2513 3624 3709 4780 2237 Size: 276 1111 85 1071 3020 Fragments arranged by size: 3020 1111 1071 276 85 BsmI GAATG_Cn' Cuts at: 2158 3074 3167 2158 Size: 916 93 4554 Fragments arranged by size: 4554 916 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2237 2257 2513 2882 3624 3709 Size: 399 240 207 20 256 369 742 85 Cuts at: 3709 4780 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 369 256 240 207 85 20 BspEI T'CCGG_A Cuts at: 2388 5226 2388 Size: 2838 2725 BspHI T'CATG_A Cuts at: 3452 5182 3452 Size: 1730 3833 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2531 2918 844 Size: 1687 387 3489 Fragments arranged by size: 3489 1687 387 BsrBI CCG'CTC Cuts at: 1394 1823 2076 3450 1394 Size: 429 253 1374 3507 Fragments arranged by size: 3507 1374 429 253 BsrDI GCAATG_nn' Cuts at: 65 1248 4058 4232 65 Size: 1183 2810 174 1396 Fragments arranged by size: 2810 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4207 4394 1700 Size: 110 2397 187 2869 Fragments arranged by size: 2869 2397 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5563 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5563 BssSI C'ACGA_G Cuts at: 3623 4635 3623 Size: 1012 4551 BstAPI GCAn_nnn'nTGC Cuts at: 794 2845 794 Size: 2051 3512 BstBI TT'CG_AA Cuts at: 4367 4367 Size: 5563 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5563 BstXI CCAn_nnnn'nTGG Cuts at: 2086 2086 Size: 5563 BstYI r'GATC_y Cuts at: 1972 2070 2893 3260 3645 3662 5114 5200 5212 Size: 98 823 367 385 17 1452 86 12 Cuts at: 5212 5558 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 823 385 367 346 98 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5011 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2034 487 Size: 683 56 808 4016 Fragments arranged by size: 4016 808 683 56 BtsI GCAGTG_nn' Cuts at: 2608 3079 3873 3893 2608 Size: 471 794 20 4278 Fragments arranged by size: 4278 794 471 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5563 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2275 2310 529 Size: 35 1711 35 3782 Fragments arranged by size: 3782 1711 35 35 DraI TTT'AAA Cuts at: 2867 2867 Size: 5563 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2669 4570 809 Size: 507 1353 1901 1802 Fragments arranged by size: 1901 1802 1353 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2205 2296 Size: 54 1425 50 275 18 345 30 91 Cuts at: 2296 2470 3010 3899 5528 8 Size: 174 540 889 1629 43 Fragments arranged by size: 1629 1425 889 540 345 275 174 91 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 3010 1537 Size: 275 1198 4090 Fragments arranged by size: 4090 1198 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2149 2849 3493 1353 Size: 796 700 644 3423 Fragments arranged by size: 3423 796 700 644 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4150 4524 4670 387 Size: 1064 138 2561 374 146 1280 Fragments arranged by size: 2561 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2522 3626 4223 5010 5278 1239 Size: 393 890 1104 597 787 268 1524 Fragments arranged by size: 1524 1104 890 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2197 2266 2197 Size: 69 5494 EcoRI G'AATT_C Cuts at: 2872 3250 2872 Size: 378 5185 FspI TGC'GCA Cuts at: 4069 4069 Size: 5563 HaeII r_GCGC'y Cuts at: 1678 4710 1678 Size: 3032 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2045 2077 2172 2204 4204 Size: 32 135 32 473 32 95 32 2000 Cuts at: 4204 4236 4278 4310 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2000 473 135 95 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2907 3153 669 Size: 988 281 969 246 3079 Fragments arranged by size: 3079 988 969 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5563 HpaI GTT'AAC Cuts at: 3153 3153 Size: 5563 KpnI G_GTAC'C Cuts at: 2888 2888 Size: 5563 MfeI C'AATT_G Cuts at: 3162 3162 Size: 5563 MluI A'CGCG_T Cuts at: 2210 5439 2210 Size: 3229 2334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2400 2692 4677 4861 1305 Size: 1095 292 1985 184 2007 Fragments arranged by size: 2007 1985 1095 292 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2177 2298 2472 10 Size: 54 1425 343 345 121 174 3101 Fragments arranged by size: 3101 1425 345 343 174 121 54 MslI CAynn'nnrTG Cuts at: 518 1166 2084 3521 3880 4039 518 Size: 648 918 1437 359 159 2042 Fragments arranged by size: 2042 1437 918 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2842 2979 3656 4804 5049 1606 Size: 531 705 137 677 1148 245 2120 Fragments arranged by size: 2120 1148 705 677 531 245 137 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5011 NdeI CA'TA_TG Cuts at: 387 387 Size: 5563 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5563 NotI GC'GGCC_GC Cuts at: 3010 3010 Size: 5563 NsiI A_TGCA'T Cuts at: 2062 2062 Size: 5563 NspI r_CATG'y Cuts at: 1132 1229 2282 2605 2638 2921 4466 1132 Size: 97 1053 323 33 283 1545 2229 Fragments arranged by size: 2229 1545 1053 323 283 97 33 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5563 PciI A'CATG_T Cuts at: 2601 2634 4462 2601 Size: 33 1828 3702 Fragments arranged by size: 3702 1828 33 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5563 PfoI T'CCnGG_A Cuts at: 2499 2531 2499 Size: 32 5531 PmeI GTTT'AAAC Cuts at: 2867 2867 Size: 5563 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3632 3664 5145 5177 3632 Size: 32 1481 32 4018 Fragments arranged by size: 4018 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2197 2266 2197 Size: 69 5494 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5563 PsiI TTA'TAA Cuts at: 3133 3133 Size: 5563 PstI C_TGCA'G Cuts at: 830 2915 830 Size: 2085 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5563 PvuII CAG'CTG Cuts at: 2137 2842 2137 Size: 705 4858 SalI G'TCGA_C Cuts at: 1936 2905 1936 Size: 969 4594 SanDI GG'GwC_CC Cuts at: 2266 2266 Size: 5563 SbfI CC_TGCA'GG Cuts at: 2915 2915 Size: 5563 ScaI AGT'ACT Cuts at: 3811 5523 3811 Size: 1712 3851 SfcI C'TryA_G Cuts at: 826 1045 2911 4046 4727 4918 826 Size: 219 1866 1135 681 191 1471 Fragments arranged by size: 1866 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5563 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5563 SmaI CCC'GGG Cuts at: 1872 2890 1872 Size: 1018 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3667 4568 4830 820 Size: 197 926 1724 901 262 1553 Fragments arranged by size: 1724 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3354 493 Size: 2861 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5563 SphI G_CATG'C Cuts at: 1132 2282 2921 1132 Size: 1150 639 3774 Fragments arranged by size: 3774 1150 639 SspI AAT'ATT Cuts at: 5 52 3487 5 Size: 47 3435 2081 Fragments arranged by size: 3435 2081 47 StuI AGG'CCT Cuts at: 1517 2247 1517 Size: 730 4833 StyI C'CwwG_G Cuts at: 513 1065 3002 513 Size: 552 1937 3074 Fragments arranged by size: 3074 1937 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2632 3590 3607 3760 3945 4390 325 Size: 1255 1052 958 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1052 958 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3809 5521 96 Size: 274 80 33 51 1443 1832 1712 138 Fragments arranged by size: 1832 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2123 2155 5523 5555 2123 Size: 32 3368 32 2131 Fragments arranged by size: 3368 2131 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5563 XbaI T'CTAG_A Cuts at: 2899 2899 Size: 5563 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2497 2497 Size: 5563 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5563 XmaI C'CCGG_G Cuts at: 1870 2888 1870 Size: 1018 4545 XmnI GAAnn'nnTTC Cuts at: 2189 3692 2189 Size: 1503 4060 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3371 276 Size: 53 83 186 936 1837 2468 Fragments arranged by size: 2468 1837 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI