################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflIII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BamHI BbeI BbrPI BbvCI BcgI BglII BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV FauI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 585 GTATAC GT^[AC][GT]AC AfaI 498 GTAC GT^AC AflII 195 CTTAAG C^TTAAG Aor51HI 7 AGCGCT AGC^GCT ApaI 891 GGGCCC GGGCC^C AspHI 875 GTGCTC G[AT]GC[AT]^C BalI 707 TGGCCA TGG^CCA BanI 383 GGTGCC G^G[CT][GA]CC BanII 891 GGGCCC G[GA]GC[CT]^C BciVI 477 GTATCC GTATCC(6/5) BfrI 195 CTTAAG C^TTAAG BlpI 3 GCTGAGC GC^T[ACGT]AGC BpmI 87 CTGGAG CTGGAG(16/14) Bpu1102I 3 GCTGAGC GC^T[ACGT]AGC BsiEI 330 CGATCG CG[GA][CT]^CG BsiHKAI 875 GTGCTC G[AT]GC[AT]^C BsmI 470 GCATTC GAATGC(1/-1) Bst1107I 585 GTATAC GTA^TAC BstXI 612 CCATTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 854 AGATCC [GA]^GATC[CT] BstZ17I 585 GTATAC GTA^TAC CelII 3 GCTGAGC GC^T[ACGT]AGC EaeI 707 TGGCCA [CT]^GGCC[GA] EarI 797 CTCTTC CTCTTC(1/4) Eco47III 7 AGCGCT AGC^GCT EcoNI 451 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 506 ATGCAT ATGCA^T HaeII 7 AGCGCT [GA]GCGC^[CT] HindIII 198 AAGCTT A^AGCTT Ksp632I 797 CTCTTC CTCTTC(1/4) MaeIII 414 GTTAC ^GT[ACGT]AC MflI 854 AGATCC [GA]^GATC[CT] MluNI 707 TGGCCA TGG^CCA MscI 707 TGGCCA TGG^CCA MspA1I 617 CAGCTG C[AC]G^C[GT]G NsiI 506 ATGCAT ATGCA^T PflMI 272 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 375 GAGTC GAGTC(4/5) PpuMI 254 GGGTCCT [GA]G^G[AT]CC[CT] PstI 172 CTGCAG CTGCA^G PvuI 330 CGATCG CGAT^CG PvuII 617 CAGCTG CAG^CTG RsaI 498 GTAC GT^AC SexAI 692 ACCTGGT A^CC[AT]GGT SfcI 172 CTGCAG C^T[GA][CT]AG SmlI 195 CTTAAG C^T[CT][GA]AG TaqI 329 TCGA T^CGA TfiI 825 GATTC G^A[AT]TC TthHB8I 329 TCGA T^CGA Van91I 272 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 854 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 46 GAATTT [GA]^AATT[CT] 112 AAATTC AlwI 855 GATCC GGATC(4/5) 104 GGATC AlwNI 175 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 496 CAGTACCTG ApoI 46 GAATTT [GA]^AATT[CT] 112 AAATTC AvrII 15 CCTAGG C^CTAGG 829 CCTAGG BclI 182 TGATCA T^GATCA 815 TGATCA BcnI 336 CCCGG CC^[GC]GG 636 CCGGG BfaI 16 CTAG C^TAG 830 CTAG BglI 149 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 386 GCCTTCTGGGC BlnI 15 CCTAGG C^CTAGG 829 CCTAGG BmyI 875 GTGCTC G[AGT]GC[ACT]^C 891 GGGCCC Bsp1286I 875 GTGCTC G[AGT]GC[ACT]^C 891 GGGCCC BsrI 119 ACTGG ACTGG(1/-1) 755 CCAGT CfoI 8 GCGC GCG^C 658 GCGC FbaI 182 TGATCA T^GATCA 815 TGATCA HapII 337 CCGG C^CGG 636 CCGG HhaI 8 GCGC GCG^C 658 GCGC HinP1I 8 GCGC G^CGC 658 GCGC HinfI 375 GAGTC G^A[ACGT]TC 825 GATTC HpaII 337 CCGG C^CGG 636 CCGG MaeI 16 CTAG C^TAG 830 CTAG MspI 337 CCGG C^CGG 636 CCGG NciI 336 CCCGG CC^[GC]GG 636 CCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AluI AvaII BbsI BbvI BpuAI BsaJI BsiYI BslI BsmAI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HphI ItaI MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp509I TspRI