(Circular) (Six-base) MAPSORT of: pFN21AE5324.seq Check: 7767 from: 1 to: 5602 pFN21AE5324 5602 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3412 278 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2591 2945 1937 Size: 654 354 4594 Fragments arranged by size: 4594 654 354 Acc65I G'GTAC_C Cuts at: 2923 2923 Size: 5602 AclI AA'CG_TT Cuts at: 1563 3729 4102 1563 Size: 2166 373 3063 Fragments arranged by size: 3063 2166 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2378 2687 3665 5049 5317 1632 Size: 746 309 978 1384 268 1917 Fragments arranged by size: 1917 1384 978 746 309 268 AfeI AGC'GCT Cuts at: 2014 2014 Size: 5602 AflII C'TTAA_G Cuts at: 820 1017 2200 820 Size: 197 1183 4222 Fragments arranged by size: 4222 1183 197 AflIII A'CryG_T Cuts at: 1203 4501 5478 1203 Size: 3298 977 1327 Fragments arranged by size: 3298 1327 977 AgeI A'CCGG_T Cuts at: 4433 4433 Size: 5602 AhdI GACnn_n'nnGTC Cuts at: 4331 4331 Size: 5602 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5602 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5602 AlwNI CAG_nnn'CTG Cuts at: 2185 2506 3345 4917 2185 Size: 321 839 1572 2870 Fragments arranged by size: 2870 1572 839 321 ApaI G_GGCC'C Cuts at: 2900 2900 Size: 5602 ApaLI G'TGCA_C Cuts at: 3659 4815 3659 Size: 1156 4446 ApoI r'AATT_y Cuts at: 1493 2051 2117 2911 3137 3289 5398 1493 Size: 558 66 794 226 152 2109 1697 Fragments arranged by size: 2109 1697 794 558 226 152 66 AseI AT'TA_AT Cuts at: 160 1032 4156 160 Size: 872 3124 1606 Fragments arranged by size: 3124 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 AvaI C'yCGr_G Cuts at: 1870 1943 2927 1870 Size: 73 984 4545 Fragments arranged by size: 4545 984 73 AvrII C'CTAG_G Cuts at: 2020 2834 2020 Size: 814 4788 BamHI G'GATC_C Cuts at: 2932 3299 2932 Size: 367 5235 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2388 2923 4378 618 Size: 325 217 627 601 535 1455 1842 Fragments arranged by size: 1842 1455 627 601 535 325 217 BanII G_rGCy'C Cuts at: 1391 2900 2921 1391 Size: 1509 21 4072 Fragments arranged by size: 4072 1509 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2299 2758 2876 928 Size: 971 400 459 118 3654 Fragments arranged by size: 3654 971 459 400 118 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3793 3827 1903 Size: 34 50 34 1772 34 3678 Fragments arranged by size: 3678 1772 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2493 3494 4704 2493 Size: 1001 1210 3391 Fragments arranged by size: 3391 1210 1001 BclI T'GATC_A Cuts at: 1548 2187 2820 1548 Size: 639 633 4330 Fragments arranged by size: 4330 639 633 BfrBI ATG'CAT Cuts at: 2513 2513 Size: 5602 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2160 2397 4213 Size: 107 122 71 631 605 488 237 1816 Cuts at: 4213 136 Size: 1525 Fragments arranged by size: 1816 1525 631 605 488 237 122 107 71 BglII A'GATC_T Cuts at: 5597 5597 Size: 5602 BlpI GC'TnA_GC Cuts at: 2009 3019 2009 Size: 1010 4592 Bme1580I G_kGCm'C Cuts at: 1790 2900 3663 4819 1790 Size: 1110 763 1156 2573 Fragments arranged by size: 2573 1156 1110 763 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5602 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2894 3333 4291 Size: 416 53 83 399 24 1477 439 958 Cuts at: 4291 442 Size: 1753 Fragments arranged by size: 1753 1477 958 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2113 4262 1239 Size: 874 2149 2579 Fragments arranged by size: 2579 2149 874 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3727 4592 4890 3727 Size: 865 298 4439 Fragments arranged by size: 4439 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4265 882 Size: 617 2766 2219 Fragments arranged by size: 2766 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3393 493 Size: 711 2189 2702 Fragments arranged by size: 2702 2189 711 BsaBI GATnn'nnATC Cuts at: 5596 5596 Size: 5602 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3409 3791 4426 275 Size: 53 83 186 936 1876 382 635 1451 Fragments arranged by size: 1876 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4035 4433 4707 4854 5265 4035 Size: 398 274 147 411 4372 Fragments arranged by size: 4372 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2731 2761 1178 Size: 30 165 30 1328 30 4019 Fragments arranged by size: 4019 1328 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5602 BseYI C'CCAG_C Cuts at: 2892 4805 5301 2892 Size: 1913 496 3193 Fragments arranged by size: 3193 1913 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5029 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2338 3052 3813 4841 1540 Size: 275 186 337 714 761 1028 2301 Fragments arranged by size: 2301 1028 761 714 337 275 186 BsiHKAI G_wGCw'C Cuts at: 2884 3663 3748 4819 2884 Size: 779 85 1071 3667 Fragments arranged by size: 3667 1071 779 85 BsmI GAATG_Cn' Cuts at: 2475 3113 3206 2475 Size: 638 93 4871 Fragments arranged by size: 4871 638 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2884 2900 2921 3663 3748 4819 1391 Size: 399 1094 16 21 742 85 1071 2174 Fragments arranged by size: 2174 1094 1071 742 399 85 21 16 BspEI T'CCGG_A Cuts at: 5265 5265 Size: 5602 BspHI T'CATG_A Cuts at: 3491 5221 3491 Size: 1730 3872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2957 844 Size: 2113 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3489 1394 Size: 429 1666 3507 Fragments arranged by size: 3507 1666 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4097 4271 65 Size: 1183 2849 174 1396 Fragments arranged by size: 2849 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4246 4433 1700 Size: 110 2436 187 2869 Fragments arranged by size: 2869 2436 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5602 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5602 BssSI C'ACGA_G Cuts at: 3662 4674 3662 Size: 1012 4590 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5602 BstBI TT'CG_AA Cuts at: 4406 4406 Size: 5602 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5602 BstXI CCAn_nnnn'nTGG Cuts at: 2624 2624 Size: 5602 BstYI r'GATC_y Cuts at: 1972 2859 2932 3299 3684 3701 5153 5239 5251 Size: 887 73 367 385 17 1452 86 12 Cuts at: 5251 5597 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 887 385 367 346 86 73 17 12 BstZ17I GTA'TAC Cuts at: 2592 2592 Size: 5602 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5050 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4863 Fragments arranged by size: 4863 683 56 BtsI GCAGTG_nn' Cuts at: 2049 2186 2658 3118 3912 3932 2049 Size: 137 472 460 794 20 3719 Fragments arranged by size: 3719 794 472 460 137 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5602 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5567 DraI TTT'AAA Cuts at: 2906 2906 Size: 5602 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4609 809 Size: 507 3293 1802 Fragments arranged by size: 3293 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2712 3049 3938 Size: 54 1425 50 275 18 882 337 889 Cuts at: 3938 5567 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 882 337 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3049 1537 Size: 275 1237 4090 Fragments arranged by size: 4090 1237 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2808 3532 1353 Size: 1455 724 3423 Fragments arranged by size: 3423 1455 724 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4189 4563 4709 387 Size: 1064 138 2600 374 146 1280 Fragments arranged by size: 2600 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2113 2378 2687 3665 4262 5049 5317 Size: 393 481 265 309 978 597 787 268 Cuts at: 5317 1239 Size: 1524 Fragments arranged by size: 1524 978 787 597 481 393 309 268 265 EcoNI CCTnn'n_nnAGG Cuts at: 2460 2460 Size: 5602 EcoO109I rG'GnC_Cy Cuts at: 2260 2367 2896 2260 Size: 107 529 4966 Fragments arranged by size: 4966 529 107 EcoRI G'AATT_C Cuts at: 2911 3289 2911 Size: 378 5224 FspI TGC'GCA Cuts at: 4108 4108 Size: 5602 HaeII r_GCGC'y Cuts at: 1678 2016 4749 1678 Size: 338 2733 2531 Fragments arranged by size: 2733 2531 338 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2094 2126 2320 2352 2731 Size: 32 135 32 522 32 194 32 379 Cuts at: 2731 2763 4243 4275 4317 4349 1373 Size: 32 1480 32 42 32 2626 Fragments arranged by size: 2626 1480 522 379 194 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2946 3192 669 Size: 988 281 1008 246 3079 Fragments arranged by size: 3079 1008 988 281 246 HindIII A'AGCT_T Cuts at: 748 2203 748 Size: 1455 4147 HpaI GTT'AAC Cuts at: 3192 3192 Size: 5602 KpnI G_GTAC'C Cuts at: 2927 2927 Size: 5602 MfeI C'AATT_G Cuts at: 3201 3201 Size: 5602 MluI A'CGCG_T Cuts at: 5478 5478 Size: 5602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4716 4900 1305 Size: 3411 184 2007 Fragments arranged by size: 3411 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2714 10 Size: 54 1425 343 882 2898 Fragments arranged by size: 2898 1425 882 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3560 3919 4078 518 Size: 648 2394 359 159 2042 Fragments arranged by size: 2394 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2624 3018 3695 4843 5088 1606 Size: 1018 394 677 1148 245 2120 Fragments arranged by size: 2120 1148 1018 677 394 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5050 NdeI CA'TA_TG Cuts at: 387 387 Size: 5602 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5602 NotI GC'GGCC_GC Cuts at: 3049 3049 Size: 5602 NsiI A_TGCA'T Cuts at: 2515 2515 Size: 5602 NspI r_CATG'y Cuts at: 1132 1229 2960 4505 1132 Size: 97 1731 1545 2229 Fragments arranged by size: 2229 1731 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5602 PciI A'CATG_T Cuts at: 4501 4501 Size: 5602 PflMI CCAn_nnn'nTGG Cuts at: 1108 2283 1108 Size: 1175 4427 PmeI GTTT'AAAC Cuts at: 2906 2906 Size: 5602 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3671 3703 5184 5216 3671 Size: 32 1481 32 4057 Fragments arranged by size: 4057 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2260 2260 Size: 5602 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5602 PsiI TTA'TAA Cuts at: 2408 3172 2408 Size: 764 4838 PspOMI G'GGCC_C Cuts at: 2896 2896 Size: 5602 PstI C_TGCA'G Cuts at: 830 2181 2954 830 Size: 1351 773 3478 Fragments arranged by size: 3478 1351 773 PvuI CG_AT'CG Cuts at: 2001 2338 2001 Size: 337 5265 PvuII CAG'CTG Cuts at: 2624 2624 Size: 5602 SalI G'TCGA_C Cuts at: 1936 2944 1936 Size: 1008 4594 SbfI CC_TGCA'GG Cuts at: 2954 2954 Size: 5602 ScaI AGT'ACT Cuts at: 3850 5562 3850 Size: 1712 3890 SexAI A'CCwGG_T Cuts at: 2697 2697 Size: 5602 SfcI C'TryA_G Cuts at: 826 1045 2177 2950 4085 4766 4957 826 Size: 219 1132 773 1135 681 191 1471 Fragments arranged by size: 1471 1135 1132 773 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5602 SmaI CCC'GGG Cuts at: 1872 2929 1872 Size: 1057 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2200 3706 4607 4869 820 Size: 197 926 257 1506 901 262 1553 Fragments arranged by size: 1553 1506 926 901 262 257 197 SnaBI TAC'GTA Cuts at: 493 3393 493 Size: 2900 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5602 SphI G_CATG'C Cuts at: 1132 2960 1132 Size: 1828 3774 SspI AAT'ATT Cuts at: 5 52 3526 5 Size: 47 3474 2081 Fragments arranged by size: 3474 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5602 StyI C'CwwG_G Cuts at: 513 1065 2020 2461 2646 2673 2834 3041 513 Size: 552 955 441 185 27 161 207 3074 Fragments arranged by size: 3074 955 552 441 207 185 161 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3629 3646 3799 3984 4429 325 Size: 1255 2049 17 153 185 445 1498 Fragments arranged by size: 2049 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3848 5560 96 Size: 274 80 33 51 1443 1871 1712 138 Fragments arranged by size: 1871 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2070 2102 5562 5594 2070 Size: 32 3460 32 2078 Fragments arranged by size: 3460 2078 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5602 XbaI T'CTAG_A Cuts at: 2938 2938 Size: 5602 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5602 XmaI C'CCGG_G Cuts at: 1870 2927 1870 Size: 1057 4545 XmnI GAAnn'nnTTC Cuts at: 3731 3731 Size: 5602 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3410 276 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI