################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI ClaI CpoI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 232 GTCTAC GT^[AC][GT]AC AciI 366 GCGG CCGC(-3/-1) AcsI 48 AAATTT [GA]^AATT[CT] AgeI 643 ACCGGT A^CCGGT AlwI 384 GATCC GGATC(4/5) AlwNI 282 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 48 AAATTT [GA]^AATT[CT] AseI 571 ATTAAT AT^TAAT AsnI 571 ATTAAT AT^TAAT AspHI 549 GAGCTC G[AT]GC[AT]^C AvaI 276 CTCGAG C^[CT]CG[GA]G BanII 549 GAGCTC G[GA]GC[CT]^C BlpI 447 GCTTAGC GC^T[ACGT]AGC BmyI 549 GAGCTC G[AGT]GC[ACT]^C Bpu1102I 447 GCTTAGC GC^T[ACGT]AGC BsaWI 643 ACCGGT [AT]^CCGG[AT] BsiHKAI 549 GAGCTC G[AT]GC[AT]^C BsiYI 644 CCGGTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 644 CCGGTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 207 CGTCTC CGTCTC(1/5) BsoBI 276 CTCGAG C^[CT]CG[GA]G Bsp1286I 549 GAGCTC G[AGT]GC[ACT]^C Bsp1407I 437 TGTACA T^GTACA BsrFI 643 ACCGGT [GA]^CCGG[CT] BsrGI 437 TGTACA T^GTACA BstYI 383 AGATCC [GA]^GATC[CT] Cac8I 399 GCTGGC GC[ACGT]^[ACGT]GC CelII 447 GCTTAGC GC^T[ACGT]AGC Cfr10I 643 ACCGGT [GA]^CCGG[CT] DpnII 384 GATC ^GATC DraI 45 TTTAAA TTT^AAA DraIII 343 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG HaeIII 402 GGCC GG^CC HapII 644 CCGG C^CGG HpaII 644 CCGG C^CGG MboI 384 GATC ^GATC MfeI 90 CAATTG C^AATTG MflI 383 AGATCC [GA]^GATC[CT] MspI 644 CCGG C^CGG MunI 90 CAATTG C^AATTG MwoI 25 GCTATTATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 384 GATC ^GATC NlaIV 778 GGGACC GG[ACGT]^[ACGT]CC PaeR7I 276 CTCGAG C^TCGAG PinAI 643 ACCGGT A^CCGGT PleI 274 GACTC GAGTC(4/5) PshBI 571 ATTAAT AT^TAAT SacI 549 GAGCTC GAGCT^C Sau3AI 384 GATC ^GATC SexAI 160 ACCAGGT A^CC[AT]GGT SspBI 437 TGTACA T^GTACA TaqI 277 TCGA T^CGA TthHB8I 277 TCGA T^CGA XcmI 389 CCAGGCTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 276 CTCGAG C^TCGAG XhoII 383 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 315 GTAC GT^AC 438 GTAC AvaII 741 GGTCC G^G[AT]CC 779 GGACC BbvI 393 GCTGC GCAGC(8/12) 396 GCTGC BpmI 559 CTCCAG CTGGAG(16/14) 818 CTCCAG BseRI 123 CTCCTC GAGGAG(10/8) 609 GAGGAG BsmFI 177 GGGAC GGGAC(10/14) 778 GGGAC BsrI 561 CCAGT ACTGG(1/-1) 744 CCAGT Cfr13I 741 GGTCC G^G[ACGT]CC 779 GGACC EarI 126 CTCTTC CTCTTC(1/4) 210 CTCTTC Fnu4HI 393 GCTGC GC^[ACGT]GC 396 GCTGC ItaI 393 GCTGC GC^[ACGT]GC 396 GCTGC Ksp632I 126 CTCTTC CTCTTC(1/4) 210 CTCTTC MslI 570 CATTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 673 CATGTAGGTG NlaIII 264 CATG CATG^ 673 CATG RsaI 315 GTAC GT^AC 438 GTAC Sau96I 741 GGTCC G^G[ACGT]CC 779 GGACC TseI 393 GCTGC G^C[AT]GC 396 GCTGC Tsp509I 49 AATT ^AATT 91 AATT TspRI 83 CAGTG CA[GC]TG[ACGT][ACGT]^ 347 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AluI BsaJI BsmAI BssKI BstF5I BstNI DdeI EcoRII FokI HinfI HphI MaeIII MboII MnlI MseI MvaI ScrFI SfaNI SmlI TfiI Tru9I Tsp45I