################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbrPI BbvCI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsiEI BsiWI BsmI BspDI BspHI BspLU11I BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstYI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FbaI FseI FspI HpaI KpnI MamI MfeI MflI MluI MunI NcoI NheI NotI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SfiI SfuI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XhoII ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccIII 1130 TCCGGA T^CCGGA AfaI 642 GTAC GT^AC AlwNI 111 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 548 AGCGCT AGC^GCT Asp700I 324 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 679 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 6 GGCGCC GGCGC^C BbsI 277 GTCTTC GAAGAC(2/6) BcgI 802 GCAGACCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 763 GGATAC GTATCC(6/5) BpuAI 277 GTCTTC GAAGAC(2/6) BsaI 415 GAGACC GGTCTC(1/5) BsaWI 1130 TCCGGA [AT]^CCGG[AT] BseAI 1130 TCCGGA T^CCGGA BsgI 1605 GTGCAG GTGCAG(16/14) Bsp1407I 641 TGTACA T^GTACA BspEI 1130 TCCGGA T^CCGGA BsrFI 307 GCCGGC [GA]^CCGG[CT] BsrGI 641 TGTACA T^GTACA BstEII 1647 GGTGACC G^GT[ACGT]ACC BstPI 1647 GGTGACC G^GT[ACGT]ACC Cfr10I 307 GCCGGC [GA]^CCGG[CT] EarI 1207 GAAGAG CTCTTC(1/4) Eco47III 548 AGCGCT AGC^GCT EcoO65I 1647 GGTGACC G^GT[ACGT]ACC EcoRI 1244 GAATTC G^AATTC HincII 1123 GTCAAC GT[CT]^[GA]AC HindII 1123 GTCAAC GT[CT]^[GA]AC KasI 6 GGCGCC G^GCGCC Ksp632I 1207 GAAGAG CTCTTC(1/4) KspI 239 CCGCGG CCGC^GG MroI 1130 TCCGGA T^CCGGA MseI 1718 TTAA T^TAA NaeI 307 GCCGGC GCC^GGC NarI 6 GGCGCC GG^CGCC NdeI 964 CATATG CA^TATG NgoMIV 307 GCCGGC G^CCGGC NruI 102 TCGCGA TCG^CGA PaeR7I 1380 CTCGAG C^TCGAG PflFI 679 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 1396 CAGCTG CAG^CTG RsaI 642 GTAC GT^AC SacII 239 CCGCGG CCGC^GG SexAI 845 ACCTGGT A^CC[AT]GGT SfoI 6 GGCGCC GGC^GCC SgrAI 306 CGCCGGCG C[GA]^CCGG[CT]G SmaI 1592 CCCGGG CCC^GGG SspBI 641 TGTACA T^GTACA TfiI 1104 GAATC G^A[AT]TC Tru9I 1718 TTAA T^TAA Tth111I 679 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1380 CTCGAG C^TCGAG XmaCI 1592 CCCGGG C^CCGGG XmaI 1592 CCCGGG C^CCGGG XmnI 324 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AspHI 58 GAGCTC G[AT]GC[AT]^C 754 GAGCTC BalI 888 TGGCCA TGG^CCA 1637 TGGCCA BanI 6 GGCGCC G^G[CT][GA]CC 1363 GGTGCC BlpI 1029 GCTTAGC GC^T[ACGT]AGC 1570 GCTGAGC Bpu1102I 1029 GCTTAGC GC^T[ACGT]AGC 1570 GCTGAGC BsiHKAI 58 GAGCTC G[AT]GC[AT]^C 754 GAGCTC BsrBI 1803 CCGCTC CCGCTC(-3/-3) 257 GAGCGG BstXI 687 CCAGAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1670 CCATCACCCTGG CelII 1029 GCTTAGC GC^T[ACGT]AGC 1570 GCTGAGC EcoNI 555 CCTTCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1353 CCTGGTTCAGG EcoT14I 143 CCAAGG C^C[AT][AT]GG 768 CCTTGG HindIII 1684 AAGCTT A^AGCTT 1744 AAGCTT MluNI 888 TGGCCA TGG^CCA 1637 TGGCCA MscI 888 TGGCCA TGG^CCA 1637 TGGCCA NspI 510 GCATGT [GA]CATG^[CT] 1289 ACATGC PflMI 380 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1560 CCAGAGAGTGG PpuMI 261 GGGACCT [GA]G^G[AT]CC[CT] 881 AGGTCCC SacI 58 GAGCTC GAGCT^C 754 GAGCTC SmlI 1380 CTCGAG C^T[CT][GA]AG 1747 CTTGAG StyI 143 CCAAGG C^C[AT][AT]GG 768 CCTTGG TaqI 56 TCGA T^CGA 1381 TCGA TthHB8I 56 TCGA T^CGA 1381 TCGA Van91I 380 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1560 CCAGAGAGTGG XbaI 816 TCTAGA T^CTAGA 1107 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AcsI AcyI AluI AlwI AocI ApaI ApoI AvaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp45I Tsp509I TspRI XcmI