(Circular) (Six-base) MAPSORT of: pFN21AE3261.seq Check: 5653 from: 1 to: 6583 pFN21AE3261 6583 bp With 182 enzymes: SgfI * March 28, 2012 14:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2409 2409 Size: 6583 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4393 278 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3926 1937 Size: 1989 4594 Acc65I G'GTAC_C Cuts at: 3904 3904 Size: 6583 AclI AA'CG_TT Cuts at: 1563 4710 5083 1563 Size: 3147 373 3063 Fragments arranged by size: 3147 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3323 4646 6030 6298 1632 Size: 1691 1323 1384 268 1917 Fragments arranged by size: 1917 1691 1384 1323 268 AfeI AGC'GCT Cuts at: 2555 2555 Size: 6583 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6386 AflIII A'CryG_T Cuts at: 1203 5482 6459 1203 Size: 4279 977 1327 Fragments arranged by size: 4279 1327 977 AgeI A'CCGG_T Cuts at: 5414 5414 Size: 6583 AhdI GACnn_n'nnGTC Cuts at: 5312 5312 Size: 6583 AleI CACnn'nnGTG Cuts at: 1166 3431 1166 Size: 2265 4318 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6583 AlwNI CAG_nnn'CTG Cuts at: 2121 4326 5898 2121 Size: 2205 1572 2806 Fragments arranged by size: 2806 2205 1572 ApaI G_GGCC'C Cuts at: 2188 2244 3869 2188 Size: 56 1625 4902 Fragments arranged by size: 4902 1625 56 ApaLI G'TGCA_C Cuts at: 4640 5796 4640 Size: 1156 5427 ApoI r'AATT_y Cuts at: 1493 2330 3059 3249 3523 3892 4118 4270 6379 Size: 837 729 190 274 369 226 152 2109 Cuts at: 6379 1493 Size: 1697 Fragments arranged by size: 2109 1697 837 729 369 274 226 190 152 AseI AT'TA_AT Cuts at: 160 1032 5137 160 Size: 872 4105 1606 Fragments arranged by size: 4105 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 AvaI C'yCGr_G Cuts at: 1870 1943 2259 2877 3385 3597 3908 1870 Size: 73 316 618 508 212 311 4545 Fragments arranged by size: 4545 618 508 316 311 212 73 BamHI G'GATC_C Cuts at: 3913 4280 3913 Size: 367 6216 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 3368 3904 5359 618 Size: 325 217 627 224 1357 536 1455 1842 Fragments arranged by size: 1842 1455 1357 627 536 325 224 217 BanII G_rGCy'C Cuts at: 1391 2067 2188 2244 2627 2763 2884 3869 3902 Size: 676 121 56 383 136 121 985 33 Cuts at: 3902 1391 Size: 4072 Fragments arranged by size: 4072 985 676 383 136 121 121 56 33 BbeI G_GCGC'C Cuts at: 2015 2015 Size: 6583 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2275 928 Size: 971 376 5236 Fragments arranged by size: 5236 971 376 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2796 2830 4774 4808 1903 Size: 34 859 34 1944 34 3678 Fragments arranged by size: 3678 1944 859 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2762 4475 5685 2762 Size: 1713 1210 3660 Fragments arranged by size: 3660 1713 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6583 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5194 136 Size: 107 122 71 631 605 337 3185 1525 Fragments arranged by size: 3185 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6578 6578 Size: 6583 BlpI GC'TnA_GC Cuts at: 3035 3576 4000 3035 Size: 541 424 5618 Fragments arranged by size: 5618 541 424 Bme1580I G_kGCm'C Cuts at: 1790 2105 2141 2188 2244 2500 3869 3878 4644 Size: 315 36 47 56 256 1369 9 766 Cuts at: 4644 5800 1790 Size: 1156 2573 Fragments arranged by size: 2573 1369 1156 766 315 256 56 47 36 9 BmgBI CAC'GTC Cuts at: 1318 2173 3619 1318 Size: 855 1446 4282 Fragments arranged by size: 4282 1446 855 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2456 2593 2745 Size: 416 53 83 399 24 1039 137 152 Cuts at: 2745 3863 4314 5272 442 Size: 1118 451 958 1753 Fragments arranged by size: 1753 1118 1039 958 451 416 399 152 137 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6583 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2696 2778 3704 3857 5243 1239 Size: 1457 82 926 153 1386 2579 Fragments arranged by size: 2579 1457 1386 926 153 82 Bpu10I CC'TnA_GC Cuts at: 2626 2883 2961 2626 Size: 257 78 6248 Fragments arranged by size: 6248 257 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3773 4708 5573 5871 3773 Size: 935 865 298 4485 Fragments arranged by size: 4485 935 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2414 5246 882 Size: 617 915 2832 2219 Fragments arranged by size: 2832 2219 915 617 BsaAI yAC'GTr Cuts at: 493 1204 4374 493 Size: 711 3170 2702 Fragments arranged by size: 3170 2702 711 BsaBI GATnn'nnATC Cuts at: 6577 6577 Size: 6583 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2280 2528 4390 Size: 53 83 186 936 479 268 248 1862 Cuts at: 4390 4772 5407 275 Size: 382 635 1451 Fragments arranged by size: 1862 1451 936 635 479 382 268 248 186 83 53 BsaWI w'CCGG_w Cuts at: 3135 5016 5414 5688 5835 6246 3135 Size: 1881 398 274 147 411 3472 Fragments arranged by size: 3472 1881 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2067 2097 3752 3782 1178 Size: 30 165 30 664 30 1655 30 3979 Fragments arranged by size: 3979 1655 664 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2339 2825 3848 1186 Size: 1153 486 1023 3921 Fragments arranged by size: 3921 1153 1023 486 BseYI C'CCAG_C Cuts at: 2134 2375 3283 5786 6282 2134 Size: 241 908 2503 496 2435 Fragments arranged by size: 2503 2435 908 496 241 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3631 1163 Size: 573 1895 4115 Fragments arranged by size: 4115 1895 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4033 4794 5822 1540 Size: 275 186 2032 761 1028 2301 Fragments arranged by size: 2301 2032 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2067 2763 4644 4729 5800 2067 Size: 696 1881 85 1071 2850 Fragments arranged by size: 2850 1881 1071 696 85 BsmI GAATG_Cn' Cuts at: 4094 4187 4094 Size: 93 6490 BsmBI CGTCTCn'nnnn_ Cuts at: 2029 2161 2386 2029 Size: 132 225 6226 Fragments arranged by size: 6226 225 132 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2105 2141 2188 2244 2500 2627 Size: 399 277 38 36 47 56 256 127 Cuts at: 2627 2763 2884 3869 3878 3902 4644 4729 5800 Size: 136 121 985 9 24 742 85 1071 Cuts at: 5800 1391 Size: 2174 Fragments arranged by size: 2174 1071 985 742 399 277 256 136 127 121 85 56 47 38 36 24 9 BspEI T'CCGG_A Cuts at: 3135 6246 3135 Size: 3111 3472 BspHI T'CATG_A Cuts at: 4472 6202 4472 Size: 1730 4853 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2409 2985 3099 3938 844 Size: 1565 576 114 839 3489 Fragments arranged by size: 3489 1565 839 576 114 BsrBI CCG'CTC Cuts at: 1394 1823 2264 3810 4470 1394 Size: 429 441 1546 660 3507 Fragments arranged by size: 3507 1546 660 441 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5078 5252 65 Size: 1183 3830 174 1396 Fragments arranged by size: 3830 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2312 5227 5414 1700 Size: 110 502 2915 187 2869 Fragments arranged by size: 2915 2869 502 187 110 BsrGI T'GTAC_A Cuts at: 96 2646 96 Size: 2550 4033 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6583 BssSI C'ACGA_G Cuts at: 4643 5655 4643 Size: 1012 5571 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6583 BstBI TT'CG_AA Cuts at: 5387 5387 Size: 6583 BstEII G'GTnAC_C Cuts at: 1184 3652 1184 Size: 2468 4115 BstXI CCAn_nnnn'nTGG Cuts at: 2699 3682 2699 Size: 983 5600 BstYI r'GATC_y Cuts at: 1972 3913 4280 4665 4682 6134 6220 6232 6578 Size: 1941 367 385 17 1452 86 12 346 Cuts at: 6578 1972 Size: 1977 Fragments arranged by size: 1977 1941 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2051 2087 2425 2904 3518 2051 Size: 36 338 479 614 5116 Fragments arranged by size: 5116 614 479 338 36 BtgI C'CryG_G Cuts at: 513 1065 2004 2244 2537 2925 3255 513 Size: 552 939 240 293 388 330 3841 Fragments arranged by size: 3841 939 552 388 330 293 240 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2124 487 Size: 683 56 898 4946 Fragments arranged by size: 4946 898 683 56 BtsI GCAGTG_nn' Cuts at: 2664 2937 4099 4893 4913 2664 Size: 273 1162 794 20 4334 Fragments arranged by size: 4334 1162 794 273 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6583 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6548 DraI TTT'AAA Cuts at: 3887 3887 Size: 6583 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5590 809 Size: 507 4274 1802 Fragments arranged by size: 4274 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2782 2893 3438 Size: 54 1425 50 275 18 952 111 545 Cuts at: 3438 3642 3805 4030 4919 6548 8 Size: 204 163 225 889 1629 43 Fragments arranged by size: 1629 1425 952 889 545 275 225 204 163 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4030 1537 Size: 275 2218 4090 Fragments arranged by size: 4090 2218 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3207 4513 1353 Size: 1854 1306 3423 Fragments arranged by size: 3423 1854 1306 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2285 3428 3512 5170 5544 5690 Size: 1064 138 696 1143 84 1658 374 146 Cuts at: 5690 387 Size: 1280 Fragments arranged by size: 1658 1280 1143 1064 696 374 146 138 84 EcoICRI GAG'CTC Cuts at: 2065 2761 2065 Size: 696 5887 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2696 2778 3323 3704 3857 4646 5243 Size: 393 1064 82 545 381 153 789 597 Cuts at: 5243 6030 6298 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1064 789 787 597 545 393 381 268 153 82 EcoNI CCTnn'n_nnAGG Cuts at: 2564 3362 2564 Size: 798 5785 EcoO109I rG'GnC_Cy Cuts at: 2091 2184 2185 2240 2267 2706 2887 3508 3849 Size: 93 1 55 27 439 181 621 341 Cuts at: 3849 2091 Size: 4825 Fragments arranged by size: 4825 621 439 341 181 93 55 27 1 EcoRI G'AATT_C Cuts at: 3249 3892 4270 3249 Size: 643 378 5562 Fragments arranged by size: 5562 643 378 FspI TGC'GCA Cuts at: 5089 5089 Size: 6583 HaeII r_GCGC'y Cuts at: 1678 2015 2319 2557 5730 1678 Size: 337 304 238 3173 2531 Fragments arranged by size: 3173 2531 337 304 238 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2061 2067 2093 2099 3574 Size: 32 135 32 489 6 26 6 1475 Cuts at: 3574 3606 5224 5256 5298 5330 1373 Size: 32 1618 32 42 32 2626 Fragments arranged by size: 2626 1618 1475 489 135 42 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3130 3927 4173 669 Size: 988 281 1192 797 246 3079 Fragments arranged by size: 3079 1192 988 797 281 246 HindIII A'AGCT_T Cuts at: 748 3689 3749 748 Size: 2941 60 3582 Fragments arranged by size: 3582 2941 60 HpaI GTT'AAC Cuts at: 4173 4173 Size: 6583 KasI G'GCGC_C Cuts at: 2011 2011 Size: 6583 KpnI G_GTAC'C Cuts at: 3908 3908 Size: 6583 MfeI C'AATT_G Cuts at: 4182 4182 Size: 6583 MluI A'CGCG_T Cuts at: 6459 6459 Size: 6583 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2836 3103 3774 5697 5881 1305 Size: 1531 267 671 1923 184 2007 Fragments arranged by size: 2007 1923 1531 671 267 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2895 3644 10 Size: 54 1425 343 1063 749 2949 Fragments arranged by size: 2949 1425 1063 749 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2924 3431 3545 3650 3788 4541 4900 Size: 648 1758 507 114 105 138 753 359 Cuts at: 4900 5059 518 Size: 159 2042 Fragments arranged by size: 2042 1758 753 648 507 359 159 138 114 105 MspA1I CmG'CkG Cuts at: 1606 2217 2223 2246 3403 3463 3999 4676 5824 Size: 611 6 23 1157 60 536 677 1148 Cuts at: 5824 6069 1606 Size: 245 2120 Fragments arranged by size: 2120 1157 1148 677 611 536 245 60 23 6 NaeI GCC'GGC Cuts at: 2314 2314 Size: 6583 NarI GG'CG_CC Cuts at: 2012 2012 Size: 6583 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5092 Fragments arranged by size: 5092 939 552 NdeI CA'TA_TG Cuts at: 387 2970 387 Size: 2583 4000 NgoMIV G'CCGG_C Cuts at: 2312 2312 Size: 6583 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6583 NotI GC'GGCC_GC Cuts at: 4030 4030 Size: 6583 NruI TCG'CGA Cuts at: 2109 2109 Size: 6583 NspI r_CATG'y Cuts at: 1132 1229 2519 3298 3941 5486 1132 Size: 97 1290 779 643 1545 2229 Fragments arranged by size: 2229 1545 1290 779 643 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6583 PciI A'CATG_T Cuts at: 5482 5482 Size: 6583 PflMI CCAn_nnn'nTGG Cuts at: 1108 2391 3571 1108 Size: 1283 1180 4120 Fragments arranged by size: 4120 1283 1180 PfoI T'CCnGG_A Cuts at: 2673 3834 2673 Size: 1161 5422 PmeI GTTT'AAAC Cuts at: 3887 3887 Size: 6583 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4652 4684 6165 6197 4652 Size: 32 1481 32 5038 Fragments arranged by size: 5038 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2267 2887 2267 Size: 620 5963 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6583 PsiI TTA'TAA Cuts at: 4153 4153 Size: 6583 PspOMI G'GGCC_C Cuts at: 2184 2240 3865 2184 Size: 56 1625 4902 Fragments arranged by size: 4902 1625 56 PspXI vC'TCGA_Gb Cuts at: 3385 3385 Size: 6583 PstI C_TGCA'G Cuts at: 830 2222 3408 3507 3935 830 Size: 1392 1186 99 428 3478 Fragments arranged by size: 3478 1392 1186 428 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6583 PvuII CAG'CTG Cuts at: 3403 3403 Size: 6583 SacI G_AGCT'C Cuts at: 2067 2763 2067 Size: 696 5887 SacII CC_GC'GG Cuts at: 2247 2247 Size: 6583 SalI G'TCGA_C Cuts at: 1936 3925 1936 Size: 1989 4594 SbfI CC_TGCA'GG Cuts at: 3935 3935 Size: 6583 ScaI AGT'ACT Cuts at: 4831 6543 4831 Size: 1712 4871 SexAI A'CCwGG_T Cuts at: 2850 2850 Size: 6583 SfcI C'TryA_G Cuts at: 826 1045 2218 3404 3503 3931 5066 5747 5938 Size: 219 1173 1186 99 428 1135 681 191 Cuts at: 5938 826 Size: 1471 Fragments arranged by size: 1471 1186 1173 1135 681 428 219 191 99 SfoI GGC'GCC Cuts at: 2013 2013 Size: 6583 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 SgrAI Cr'CCGG_yG Cuts at: 1700 2312 1700 Size: 612 5971 SmaI CCC'GGG Cuts at: 1872 3599 3910 1872 Size: 1727 311 4545 Fragments arranged by size: 4545 1727 311 SmlI C'TyrA_G Cuts at: 820 1017 1943 3385 3752 4687 5588 5850 820 Size: 197 926 1442 367 935 901 262 1553 Fragments arranged by size: 1553 1442 935 926 901 367 262 197 SnaBI TAC'GTA Cuts at: 493 4374 493 Size: 3881 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6583 SphI G_CATG'C Cuts at: 1132 3941 1132 Size: 2809 3774 SrfI GCCC'GGGC Cuts at: 3599 3599 Size: 6583 SspI AAT'ATT Cuts at: 5 52 4507 5 Size: 47 4455 2081 Fragments arranged by size: 4455 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6583 StyI C'CwwG_G Cuts at: 513 1065 2004 2148 2773 4022 513 Size: 552 939 144 625 1249 3074 Fragments arranged by size: 3074 1249 939 625 552 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2165 4610 4627 4780 4965 5410 325 Size: 1255 585 2445 17 153 185 445 1498 Fragments arranged by size: 2445 1498 1255 585 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2646 4829 6541 Size: 274 80 33 51 1443 669 2183 1712 Cuts at: 6541 96 Size: 138 Fragments arranged by size: 2183 1712 1443 669 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3240 3272 6543 6575 3240 Size: 32 3271 32 3248 Fragments arranged by size: 3271 3248 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2687 1316 Size: 1371 5212 XbaI T'CTAG_A Cuts at: 2821 3112 3919 2821 Size: 291 807 5485 Fragments arranged by size: 5485 807 291 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3027 3193 3790 3027 Size: 166 597 5820 Fragments arranged by size: 5820 597 166 XhoI C'TCGA_G Cuts at: 1943 3385 1943 Size: 1442 5141 XmaI C'CCGG_G Cuts at: 1870 3597 3908 1870 Size: 1727 311 4545 Fragments arranged by size: 4545 1727 311 XmnI GAAnn'nnTTC Cuts at: 2333 4712 2333 Size: 2379 4204 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4391 276 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NsiI PacI PmlI PsrI RsrII SanDI SapI SfiI SwaI