(Circular) (Six-base) MAPSORT of: pFN21AE6462.seq Check: 5572 from: 1 to: 6187 pFN21AE6462 6187 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3997 278 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3530 1937 Size: 1593 4594 Acc65I G'GTAC_C Cuts at: 3508 3508 Size: 6187 AclI AA'CG_TT Cuts at: 1563 4314 4687 1563 Size: 2751 373 3063 Fragments arranged by size: 3063 2751 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2074 2591 4250 5634 5902 1632 Size: 442 517 1659 1384 268 1917 Fragments arranged by size: 1917 1659 1384 517 442 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5990 AflIII A'CryG_T Cuts at: 1203 2178 5086 6063 1203 Size: 975 2908 977 1327 Fragments arranged by size: 2908 1327 977 975 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 2995 4916 2995 Size: 1921 4266 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6187 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6187 AlwNI CAG_nnn'CTG Cuts at: 3930 5502 3930 Size: 1572 4615 ApaLI G'TGCA_C Cuts at: 4244 5400 4244 Size: 1156 5031 ApoI r'AATT_y Cuts at: 1493 2332 3496 3722 3874 5983 1493 Size: 839 1164 226 152 2109 1697 Fragments arranged by size: 2109 1697 1164 839 226 152 AseI AT'TA_AT Cuts at: 160 1032 2762 2766 3428 4741 160 Size: 872 1730 4 662 1313 1606 Fragments arranged by size: 1730 1606 1313 872 662 4 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 2713 3512 1870 Size: 73 770 799 4545 Fragments arranged by size: 4545 799 770 73 BamHI G'GATC_C Cuts at: 3517 3884 3517 Size: 367 5820 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3508 4963 618 Size: 325 217 627 1721 1455 1842 Fragments arranged by size: 1842 1721 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3249 3506 1391 Size: 1858 257 4072 Fragments arranged by size: 4072 1858 257 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2751 2790 928 Size: 971 852 39 4325 Fragments arranged by size: 4325 971 852 39 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4378 4412 1903 Size: 34 2441 34 3678 Fragments arranged by size: 3678 2441 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2260 4079 5289 2260 Size: 1819 1210 3158 Fragments arranged by size: 3158 1819 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4798 136 Size: 107 122 71 631 605 3126 1525 Fragments arranged by size: 3126 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2202 6182 2202 Size: 3980 2207 BlpI GC'TnA_GC Cuts at: 3604 3604 Size: 6187 Bme1580I G_kGCm'C Cuts at: 1790 4248 5404 1790 Size: 2458 1156 2573 Fragments arranged by size: 2573 2458 1156 BmgBI CAC'GTC Cuts at: 1318 2181 1318 Size: 863 5324 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2041 2223 3918 Size: 416 53 83 399 24 624 182 1695 Cuts at: 3918 4876 442 Size: 958 1753 Fragments arranged by size: 1753 1695 958 624 416 399 182 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6187 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3486 3486 Size: 6187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2227 2569 2895 4847 1239 Size: 988 342 326 1952 2579 Fragments arranged by size: 2579 1952 988 342 326 Bpu10I CC'TnA_GC Cuts at: 3458 3458 Size: 6187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4312 5177 5475 4312 Size: 865 298 5024 Fragments arranged by size: 5024 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2040 2549 2714 4850 882 Size: 617 541 509 165 2136 2219 Fragments arranged by size: 2219 2136 617 541 509 165 BsaAI yAC'GTr Cuts at: 493 1204 3978 493 Size: 711 2774 2702 Fragments arranged by size: 2774 2702 711 BsaBI GATnn'nnATC Cuts at: 2669 6181 2669 Size: 3512 2675 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2642 3994 4376 5011 Size: 53 83 186 936 1109 1352 382 635 Cuts at: 5011 275 Size: 1451 Fragments arranged by size: 1451 1352 1109 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4620 5018 5292 5439 5850 4620 Size: 398 274 147 411 4957 Fragments arranged by size: 4957 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2542 2572 3306 3336 1178 Size: 30 165 30 1139 30 734 30 4029 Fragments arranged by size: 4029 1139 734 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2544 1186 Size: 1358 4829 BseYI C'CCAG_C Cuts at: 5390 5886 5390 Size: 496 5691 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2253 2669 1163 Size: 573 517 416 4681 Fragments arranged by size: 4681 573 517 416 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3637 4398 5426 1540 Size: 275 186 1636 761 1028 2301 Fragments arranged by size: 2301 1636 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2366 3249 4248 4333 5404 2366 Size: 883 999 85 1071 3149 Fragments arranged by size: 3149 1071 999 883 85 BsmI GAATG_Cn' Cuts at: 3698 3791 3698 Size: 93 6094 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2366 3249 3506 4248 4333 5404 1391 Size: 399 576 883 257 742 85 1071 2174 Fragments arranged by size: 2174 1071 883 742 576 399 257 85 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 3393 4076 5806 3393 Size: 683 1730 3774 Fragments arranged by size: 3774 1730 683 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3542 844 Size: 2698 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4074 1394 Size: 429 2251 3507 Fragments arranged by size: 3507 2251 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2137 4682 4856 65 Size: 1183 889 2545 174 1396 Fragments arranged by size: 2545 1396 1183 889 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3076 4831 5018 1700 Size: 110 1266 1755 187 2869 Fragments arranged by size: 2869 1755 1266 187 110 BsrGI T'GTAC_A Cuts at: 96 3404 96 Size: 3308 2879 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6187 BssSI C'ACGA_G Cuts at: 3452 4247 5259 3452 Size: 795 1012 4380 Fragments arranged by size: 4380 1012 795 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6187 BstBI TT'CG_AA Cuts at: 4991 4991 Size: 6187 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6187 BstYI r'GATC_y Cuts at: 1972 2202 3517 3884 4269 4286 5738 5824 5836 Size: 230 1315 367 385 17 1452 86 12 Cuts at: 5836 6182 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1315 385 367 346 230 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4696 Fragments arranged by size: 4696 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2353 487 Size: 683 56 1127 4321 Fragments arranged by size: 4321 1127 683 56 BtsI GCAGTG_nn' Cuts at: 2162 3268 3327 3703 4497 4517 2162 Size: 1106 59 376 794 20 3832 Fragments arranged by size: 3832 1106 794 376 59 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6152 DraI TTT'AAA Cuts at: 3491 3491 Size: 6187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5194 809 Size: 507 3878 1802 Fragments arranged by size: 3878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2012 3273 3634 Size: 54 1425 50 275 18 182 1261 361 Cuts at: 3634 4523 6152 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1261 889 361 275 182 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3634 1537 Size: 275 1822 4090 Fragments arranged by size: 4090 1822 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2285 2371 3062 4117 1353 Size: 932 86 691 1055 3423 Fragments arranged by size: 3423 1055 932 691 86 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2996 4774 5148 5294 387 Size: 1064 138 1407 1778 374 146 1280 Fragments arranged by size: 1778 1407 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3247 3247 Size: 6187 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2074 2227 2569 2591 2895 4250 4847 Size: 393 442 153 342 22 304 1355 597 Cuts at: 4847 5634 5902 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1355 787 597 442 393 342 304 268 153 22 EcoO109I rG'GnC_Cy Cuts at: 3111 3111 Size: 6187 EcoRI G'AATT_C Cuts at: 3496 3874 3496 Size: 378 5809 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2264 2264 Size: 6187 FspI TGC'GCA Cuts at: 4693 4693 Size: 6187 HaeII r_GCGC'y Cuts at: 1678 3077 5334 1678 Size: 1399 2257 2531 Fragments arranged by size: 2531 2257 1399 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2981 3013 4828 4860 4902 Size: 32 135 32 1409 32 1815 32 42 Cuts at: 4902 4934 1373 Size: 32 2626 Fragments arranged by size: 2626 1815 1409 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3531 3777 669 Size: 988 281 1593 246 3079 Fragments arranged by size: 3079 1593 988 281 246 HindIII A'AGCT_T Cuts at: 748 2848 3267 748 Size: 2100 419 3668 Fragments arranged by size: 3668 2100 419 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KpnI G_GTAC'C Cuts at: 3512 3512 Size: 6187 MfeI C'AATT_G Cuts at: 3408 3786 3408 Size: 378 5809 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2995 5301 5485 1305 Size: 1690 2306 184 2007 Fragments arranged by size: 2306 2007 1690 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2014 3275 10 Size: 54 1425 343 182 1261 2922 Fragments arranged by size: 2922 1425 1261 343 182 54 MslI CAynn'nnrTG Cuts at: 518 1166 2061 3392 4145 4504 4663 518 Size: 648 895 1331 753 359 159 2042 Fragments arranged by size: 2042 1331 895 753 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3603 4280 5428 5673 1606 Size: 1997 677 1148 245 2120 Fragments arranged by size: 2120 1997 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4696 Fragments arranged by size: 4696 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6187 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6187 NotI GC'GGCC_GC Cuts at: 3634 3634 Size: 6187 NspI r_CATG'y Cuts at: 1132 1229 2362 2865 3545 5090 1132 Size: 97 1133 503 680 1545 2229 Fragments arranged by size: 2229 1545 1133 680 503 97 PacI TTA_AT'TAA Cuts at: 2766 2766 Size: 6187 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6187 PciI A'CATG_T Cuts at: 5086 5086 Size: 6187 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2478 1108 Size: 902 468 4817 Fragments arranged by size: 4817 902 468 PfoI T'CCnGG_A Cuts at: 2872 3348 2872 Size: 476 5711 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3063 3095 4256 4288 5769 5801 3063 Size: 32 1161 32 1481 32 3449 Fragments arranged by size: 3449 1481 1161 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3111 3111 Size: 6187 PshAI GACnn'nnGTC Cuts at: 1613 3234 1613 Size: 1621 4566 PsiI TTA'TAA Cuts at: 3757 3757 Size: 6187 PstI C_TGCA'G Cuts at: 830 2469 3322 3539 830 Size: 1639 853 217 3478 Fragments arranged by size: 3478 1639 853 217 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 SacI G_AGCT'C Cuts at: 3249 3249 Size: 6187 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SanDI GG'GwC_CC Cuts at: 3111 3111 Size: 6187 SapI GCTCTTCn'nnn_ Cuts at: 2371 2371 Size: 6187 SbfI CC_TGCA'GG Cuts at: 3539 3539 Size: 6187 ScaI AGT'ACT Cuts at: 4435 6147 4435 Size: 1712 4475 SfcI C'TryA_G Cuts at: 826 1045 2465 3062 3318 3535 4670 5351 5542 Size: 219 1420 597 256 217 1135 681 191 Cuts at: 5542 826 Size: 1471 Fragments arranged by size: 1471 1420 1135 681 597 256 219 217 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6187 SmaI CCC'GGG Cuts at: 1872 3514 1872 Size: 1642 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4291 5192 5454 820 Size: 197 926 2348 901 262 1553 Fragments arranged by size: 2348 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3978 493 Size: 3485 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6187 SphI G_CATG'C Cuts at: 1132 3545 1132 Size: 2413 3774 SspI AAT'ATT Cuts at: 5 52 3444 4111 5 Size: 47 3392 667 2081 Fragments arranged by size: 3392 2081 667 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6187 StyI C'CwwG_G Cuts at: 513 1065 2004 3626 513 Size: 552 939 1622 3074 Fragments arranged by size: 3074 1622 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4214 4231 4384 4569 5014 325 Size: 1255 2634 17 153 185 445 1498 Fragments arranged by size: 2634 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2433 3058 3404 Size: 274 80 33 51 1443 456 625 346 Cuts at: 3404 4433 6145 96 Size: 1029 1712 138 Fragments arranged by size: 1712 1443 1029 625 456 346 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2152 2184 2542 2574 2673 2705 3013 3045 3306 Size: 32 358 32 99 32 308 32 261 Cuts at: 3306 3338 6147 6179 2152 Size: 32 2809 32 2160 Fragments arranged by size: 2809 2160 358 308 261 99 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2641 1316 Size: 1325 4862 XbaI T'CTAG_A Cuts at: 3523 3523 Size: 6187 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6187 XmaI C'CCGG_G Cuts at: 1870 3512 1870 Size: 1642 4545 XmnI GAAnn'nnTTC Cuts at: 4316 4316 Size: 6187 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3995 276 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI