(Linear) (Six-base) MAPSORT of: KIEE6462.seq Check: 7840 from: 1 to: 1482 KIEE6462.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 69 586 1482 Size: 69 517 896 Fragments arranged by size: 896 517 69 AflIII A'CryG_T Cuts at: 0 173 1482 Size: 173 1309 AhdI GACnn_n'nnGTC Cuts at: 0 990 1482 Size: 990 492 ApoI r'AATT_y Cuts at: 0 327 1482 Size: 327 1155 AseI AT'TA_AT Cuts at: 0 757 761 1423 1482 Size: 757 4 662 59 Fragments arranged by size: 757 662 59 4 AvaI C'yCGr_G Cuts at: 0 708 1482 Size: 708 774 BanII G_rGCy'C Cuts at: 0 1244 1482 Size: 1244 238 BbsI GAAGACnn'nnnn_ Cuts at: 0 746 785 1482 Size: 746 39 697 Fragments arranged by size: 746 697 39 BciVI GTATCCnnnnn_n' Cuts at: 0 255 1482 Size: 255 1227 BglII A'GATC_T Cuts at: 0 197 1482 Size: 197 1285 BmgBI CAC'GTC Cuts at: 0 176 1482 Size: 176 1306 BmrI ACTGGGnnnn_n' Cuts at: 0 36 218 1482 Size: 36 182 1264 Fragments arranged by size: 1264 182 36 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1481 1482 Size: 1481 1 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 222 564 890 1482 Size: 222 342 326 592 Fragments arranged by size: 592 342 326 222 Bpu10I CC'TnA_GC Cuts at: 0 1453 1482 Size: 1453 29 BsaI GGTCTCn'nnnn_ Cuts at: 0 35 544 709 1482 Size: 35 509 165 773 Fragments arranged by size: 773 509 165 35 BsaBI GATnn'nnATC Cuts at: 0 664 1482 Size: 664 818 BsaHI Gr'CG_yC Cuts at: 0 637 1482 Size: 637 845 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 537 567 1301 1331 1482 Size: 537 30 734 30 151 Fragments arranged by size: 734 537 151 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 539 1482 Size: 539 943 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 248 664 1482 Size: 248 416 818 Fragments arranged by size: 818 416 248 BsiHKAI G_wGCw'C Cuts at: 0 361 1244 1482 Size: 361 883 238 Fragments arranged by size: 883 361 238 Bsp1286I G_dGCh'C Cuts at: 0 361 1244 1482 Size: 361 883 238 Fragments arranged by size: 883 361 238 BspHI T'CATG_A Cuts at: 0 1388 1482 Size: 1388 94 BsrDI GCAATG_nn' Cuts at: 0 132 1482 Size: 132 1350 BsrFI r'CCGG_y Cuts at: 0 1071 1482 Size: 1071 411 BsrGI T'GTAC_A Cuts at: 0 1399 1482 Size: 1399 83 BssSI C'ACGA_G Cuts at: 0 1447 1482 Size: 1447 35 BstYI r'GATC_y Cuts at: 0 197 1482 Size: 197 1285 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 348 1482 Size: 348 1134 BtsI GCAGTG_nn' Cuts at: 0 157 1263 1322 1482 Size: 157 1106 59 160 Fragments arranged by size: 1106 160 157 59 EaeI y'GGCC_r Cuts at: 0 7 1268 1482 Size: 7 1261 214 Fragments arranged by size: 1261 214 7 EarI CTCTTCn'nnn_ Cuts at: 0 280 366 1057 1482 Size: 280 86 691 425 Fragments arranged by size: 691 425 280 86 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 991 1482 Size: 991 491 EcoICRI GAG'CTC Cuts at: 0 1242 1482 Size: 1242 240 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 69 222 564 586 890 1482 Size: 69 153 342 22 304 592 Fragments arranged by size: 592 342 304 153 69 22 EcoO109I rG'GnC_Cy Cuts at: 0 1106 1482 Size: 1106 376 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 259 1482 Size: 259 1223 HaeII r_GCGC'y Cuts at: 0 1072 1482 Size: 1072 410 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 976 1008 1482 Size: 976 32 474 Fragments arranged by size: 976 474 32 HindIII A'AGCT_T Cuts at: 0 843 1262 1482 Size: 843 419 220 Fragments arranged by size: 843 419 220 MfeI C'AATT_G Cuts at: 0 1403 1482 Size: 1403 79 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 990 1482 Size: 990 492 MscI TGG'CCA Cuts at: 0 9 1270 1482 Size: 9 1261 212 Fragments arranged by size: 1261 212 9 MslI CAynn'nnrTG Cuts at: 0 56 1387 1482 Size: 56 1331 95 Fragments arranged by size: 1331 95 56 NspI r_CATG'y Cuts at: 0 357 860 1482 Size: 357 503 622 Fragments arranged by size: 622 503 357 PacI TTA_AT'TAA Cuts at: 0 761 1482 Size: 761 721 PflMI CCAn_nnn'nTGG Cuts at: 0 473 1482 Size: 473 1009 PfoI T'CCnGG_A Cuts at: 0 867 1343 1482 Size: 867 476 139 Fragments arranged by size: 867 476 139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1058 1090 1482 Size: 1058 32 392 Fragments arranged by size: 1058 392 32 PpuMI rG'GwC_Cy Cuts at: 0 1106 1482 Size: 1106 376 PshAI GACnn'nnGTC Cuts at: 0 1229 1482 Size: 1229 253 PstI C_TGCA'G Cuts at: 0 464 1317 1482 Size: 464 853 165 Fragments arranged by size: 853 464 165 SacI G_AGCT'C Cuts at: 0 1244 1482 Size: 1244 238 SanDI GG'GwC_CC Cuts at: 0 1106 1482 Size: 1106 376 SapI GCTCTTCn'nnn_ Cuts at: 0 366 1482 Size: 366 1116 SfcI C'TryA_G Cuts at: 0 460 1057 1313 1482 Size: 460 597 256 169 Fragments arranged by size: 597 460 256 169 SspI AAT'ATT Cuts at: 0 1439 1482 Size: 1439 43 TatI w'GTAC_w Cuts at: 0 428 1053 1399 1482 Size: 428 625 346 83 Fragments arranged by size: 625 428 346 83 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 147 179 537 569 668 700 1008 1040 Size: 147 32 358 32 99 32 308 32 Cuts at: 1040 1301 1333 1482 Size: 261 32 149 Fragments arranged by size: 358 308 261 149 147 99 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 636 1482 Size: 636 846 Enzymes that do cut: AcuI AflIII AhdI ApoI AseI AvaI BanII BbsI BciVI BglII BmgBI BmrI BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI BsrDI BsrFI BsrGI BssSI BstYI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HindIII MfeI MmeI MscI MslI NspI PacI PflMI PfoI PpiI PpuMI PshAI PstI SacI SanDI SapI SfcI SspI TatI TstI Tth111I Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BclI BfrBI BglI BlpI Bme1580I BmtI BpuEI BsaAI BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HpaI KasI KpnI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PasI PciI PmeI PmlI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII XbaI XcmI XhoI XmaI XmnI ZraI