################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AviII BbeI BbsI BcgI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaBI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FspI HpaI KasI KspI MamI MfeI MluI MroI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspI PacI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 1530 GGTACC G^GTACC AccI 1114 GTAGAC GT^[AC][GT]AC AcsI 655 GAATTC [GA]^AATT[CT] AcyI 336 GGCGTC G[GA]^CG[CT]C AlwNI 1283 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 616 CCTGAGG CC^T[ACGT]AGG ApoI 655 GAATTC [GA]^AATT[CT] Asp718I 1530 GGTACC G^GTACC AspI 895 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1560 CCTAGG C^CTAGG BamHI 621 GGATCC G^GATCC BanII 208 GAGCTC G[GA]GC[CT]^C BbrPI 1486 CACGTG CAC^GTG BciVI 1594 GTATCC GTATCC(6/5) BclI 287 TGATCA T^GATCA BlnI 1560 CCTAGG C^CTAGG BpmI 118 CTGGAG CTGGAG(16/14) BsaHI 336 GGCGTC G[GA]^CG[CT]C BsaWI 1502 TCCGGT [AT]^CCGG[AT] BseRI 915 CTCCTC GAGGAG(10/8) BsmI 106 GCATTC GAATGC(1/-1) BstBI 652 TTCGAA TT^CGAA BstYI 621 GGATCC [GA]^GATC[CT] Bsu36I 616 CCTGAGG CC^T[ACGT]AGG DraI 498 TTTAAA TTT^AAA DraII 611 AGGCCCC [GA]G^G[ACGT]CC[CT] Eco81I 616 CCTGAGG CC^T[ACGT]AGG EcoO109I 611 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 655 GAATTC G^AATTC FbaI 287 TGATCA T^GATCA FseI 1422 GGCCGGCC GGCCGG^CC HaeII 1285 GGCGCT [GA]GCGC^[CT] Hin1I 336 GGCGTC G[GA]^CG[CT]C HincII 1473 GTTGAC GT[CT]^[GA]AC HindII 1473 GTTGAC GT[CT]^[GA]AC HindIII 1099 AAGCTT A^AGCTT KpnI 1530 GGTACC GGTAC^C MflI 621 GGATCC [GA]^GATC[CT] MspA1I 1534 CCGCTG C[AC]G^C[GT]G NaeI 1423 GCCGGC GCC^GGC NgoMIV 1423 GCCGGC G^CCGGC NspV 652 TTCGAA TT^CGAA PaeR7I 1159 CTCGAG C^TCGAG PflFI 895 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1567 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 601 GACTC GAGTC(4/5) PmaCI 1486 CACGTG CAC^GTG PmlI 1486 CACGTG CAC^GTG SacI 208 GAGCTC GAGCT^C SfuI 652 TTCGAA TT^CGAA Tth111I 895 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1567 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1159 CTCGAG C^TCGAG XhoII 621 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 622 GATCC GGATC(4/5) 621 GGATC AspHI 208 GAGCTC G[AT]GC[AT]^C 1330 GAGCAC AvaI 1159 CTCGAG C^[CT]CG[GA]G 1441 CTCGGG BbvCI 1203 GCTGAGG CCTCAGC(-5/-2) 1375 GCTGAGG BsaI 1348 GAGACC GGTCTC(1/5) 1522 GAGACC BsgI 1315 CTGCAC GTGCAG(16/14) 1492 CTGCAC BsiHKAI 208 GAGCTC G[AT]GC[AT]^C 1330 GAGCAC BsoBI 1159 CTCGAG C^[CT]CG[GA]G 1441 CTCGGG BspMI 1409 ACCTGC ACCTGC(4/8) 294 GCAGGT BsrFI 1394 ACCGGC [GA]^CCGG[CT] 1423 GCCGGC CfoI 1272 GCGC GCG^C 1286 GCGC Cfr10I 1394 ACCGGC [GA]^CCGG[CT] 1423 GCCGGC EarI 400 GAAGAG CTCTTC(1/4) 817 GAAGAG HhaI 1272 GCGC GCG^C 1286 GCGC HinP1I 1272 GCGC G^CGC 1286 GCGC Ksp632I 400 GAAGAG CTCTTC(1/4) 817 GAAGAG NcoI 44 CCATGG C^CATGG 875 CCATGG PstI 1152 CTGCAG CTGCA^G 1537 CTGCAG SfcI 1152 CTGCAG C^T[GA][CT]AG 1537 CTGCAG TaqI 653 TCGA T^CGA 1160 TCGA Tsp45I 49 GTGAC ^GT[GC]AC 386 GTGAC TthHB8I 653 TCGA T^CGA 1160 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AfaI AluI AvaII BalI BanI BbvI BcnI BfaI BmyI BsaAI BsaJI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StuI StyI TfiI Tru9I TseI Tsp509I TspRI XcmI