(Circular) (Six-base) MAPSORT of: pFN21AE1969.seq Check: 7720 from: 1 to: 6307 pFN21AE1969 6307 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2290 2290 Size: 6307 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4117 278 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3120 3650 1937 Size: 1183 530 4594 Fragments arranged by size: 4594 1183 530 Acc65I G'GTAC_C Cuts at: 3535 3628 3535 Size: 93 6214 AclI AA'CG_TT Cuts at: 1563 4434 4807 1563 Size: 2871 373 3063 Fragments arranged by size: 3063 2871 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2302 4370 5754 6022 1632 Size: 670 2068 1384 268 1917 Fragments arranged by size: 2068 1917 1384 670 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6110 AflIII A'CryG_T Cuts at: 1203 5206 6183 1203 Size: 4003 977 1327 Fragments arranged by size: 4003 1327 977 AgeI A'CCGG_T Cuts at: 5138 5138 Size: 6307 AhdI GACnn_n'nnGTC Cuts at: 5036 5036 Size: 6307 AleI CACnn'nnGTG Cuts at: 1166 2570 2600 1166 Size: 1404 30 4873 Fragments arranged by size: 4873 1404 30 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6307 AlwNI CAG_nnn'CTG Cuts at: 3293 4050 5622 3293 Size: 757 1572 3978 Fragments arranged by size: 3978 1572 757 ApaLI G'TGCA_C Cuts at: 4364 5520 4364 Size: 1156 5151 ApoI r'AATT_y Cuts at: 1493 2660 3616 3842 3994 6103 1493 Size: 1167 956 226 152 2109 1697 Fragments arranged by size: 2109 1697 1167 956 226 152 AseI AT'TA_AT Cuts at: 160 1032 4861 160 Size: 872 3829 1606 Fragments arranged by size: 3829 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 AvaI C'yCGr_G Cuts at: 1870 1943 3164 3446 3632 1870 Size: 73 1221 282 186 4545 Fragments arranged by size: 4545 1221 282 186 73 AvrII C'CTAG_G Cuts at: 3565 3565 Size: 6307 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3519 3552 3519 Size: 33 6274 BamHI G'GATC_C Cuts at: 2626 3637 4004 2626 Size: 1011 367 4929 Fragments arranged by size: 4929 1011 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2417 2594 3314 3535 3628 Size: 325 217 627 630 177 720 221 93 Cuts at: 3628 5083 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 720 630 627 325 221 217 177 93 BanII G_rGCy'C Cuts at: 1391 2217 3626 1391 Size: 826 1409 4072 Fragments arranged by size: 4072 1409 826 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5336 BbvCI CC'TCA_GC Cuts at: 3209 3381 3209 Size: 172 6135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4498 4532 1903 Size: 34 2561 34 3678 Fragments arranged by size: 3678 2561 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3610 4199 5409 3610 Size: 589 1210 4508 Fragments arranged by size: 4508 1210 589 BclI T'GATC_A Cuts at: 1548 2292 1548 Size: 744 5563 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4918 136 Size: 107 122 71 631 605 3246 1525 Fragments arranged by size: 3246 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6302 6302 Size: 6307 BlpI GC'TnA_GC Cuts at: 3724 3724 Size: 6307 Bme1580I G_kGCm'C Cuts at: 1790 2422 3319 4368 5524 1790 Size: 632 897 1049 1156 2573 Fragments arranged by size: 2573 1156 1049 897 632 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6307 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2937 4038 4996 Size: 416 53 83 399 24 1520 1101 958 Cuts at: 4996 442 Size: 1753 Fragments arranged by size: 1753 1520 1101 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6307 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2858 2858 Size: 6307 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 4967 1239 Size: 905 2823 2579 Fragments arranged by size: 2823 2579 905 Bpu10I CC'TnA_GC Cuts at: 3209 3252 3333 3381 3209 Size: 43 81 48 6135 Fragments arranged by size: 6135 81 48 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2101 3488 4432 5297 5595 2101 Size: 1387 944 865 298 2813 Fragments arranged by size: 2813 1387 944 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3347 3521 4970 882 Size: 617 1848 174 1449 2219 Fragments arranged by size: 2219 1848 1449 617 174 BsaAI yAC'GTr Cuts at: 493 1204 2390 3411 3493 4098 493 Size: 711 1186 1021 82 605 2702 Fragments arranged by size: 2702 1186 1021 711 605 82 BsaBI GATnn'nnATC Cuts at: 6301 6301 Size: 6307 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2342 4114 4496 5131 Size: 53 83 186 936 809 1772 382 635 Cuts at: 5131 275 Size: 1451 Fragments arranged by size: 1772 1451 936 809 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3507 4740 5138 5412 5559 5970 3507 Size: 1233 398 274 147 411 3844 Fragments arranged by size: 3844 1233 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2345 2375 2903 2933 1178 Size: 30 165 30 942 30 528 30 4552 Fragments arranged by size: 4552 942 528 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2911 1186 Size: 1725 4582 BseYI C'CCAG_C Cuts at: 2963 3268 5510 6006 2963 Size: 305 2242 496 3264 Fragments arranged by size: 3264 2242 496 305 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3305 3482 1163 Size: 573 1569 177 3988 Fragments arranged by size: 3988 1569 573 177 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3757 4518 5546 1540 Size: 275 186 1756 761 1028 2301 Fragments arranged by size: 2301 1756 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2217 3339 4368 4453 5524 2217 Size: 1122 1029 85 1071 3000 Fragments arranged by size: 3000 1122 1071 1029 85 BsmI GAATG_Cn' Cuts at: 2111 3818 3911 2111 Size: 1707 93 4507 Fragments arranged by size: 4507 1707 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2745 2904 3197 2745 Size: 159 293 5855 Fragments arranged by size: 5855 293 159 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2422 3319 3339 3626 4368 4453 Size: 399 427 205 897 20 287 742 85 Cuts at: 4453 5524 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 897 742 427 399 287 205 85 20 BspEI T'CCGG_A Cuts at: 5970 5970 Size: 6307 BspHI T'CATG_A Cuts at: 4196 5926 4196 Size: 1730 4577 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2290 3423 3662 844 Size: 1446 1133 239 3489 Fragments arranged by size: 3489 1446 1133 239 BsrBI CCG'CTC Cuts at: 1394 1823 4194 1394 Size: 429 2371 3507 Fragments arranged by size: 3507 2371 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4802 4976 65 Size: 1183 3554 174 1396 Fragments arranged by size: 3554 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3399 3428 4951 5138 1700 Size: 110 1589 29 1523 187 2869 Fragments arranged by size: 2869 1589 1523 187 110 29 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6307 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6307 BssSI C'ACGA_G Cuts at: 4367 5379 4367 Size: 1012 5295 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6307 BstBI TT'CG_AA Cuts at: 2658 5111 2658 Size: 2453 3854 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6307 BstXI CCAn_nnnn'nTGG Cuts at: 2338 2572 2971 2338 Size: 234 399 5674 Fragments arranged by size: 5674 399 234 BstYI r'GATC_y Cuts at: 1972 2626 3637 4004 4389 4406 5858 5944 5956 Size: 654 1011 367 385 17 1452 86 12 Cuts at: 5956 6302 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1011 654 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2622 2622 Size: 6307 BtgI C'CryG_G Cuts at: 513 1065 2049 2880 3309 3434 513 Size: 552 984 831 429 125 3386 Fragments arranged by size: 3386 984 831 552 429 125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3010 487 Size: 683 56 1784 3784 Fragments arranged by size: 3784 1784 683 56 BtsI GCAGTG_nn' Cuts at: 2641 3482 3823 4617 4637 2641 Size: 841 341 794 20 4311 Fragments arranged by size: 4311 841 794 341 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6307 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6272 DraI TTT'AAA Cuts at: 2505 3611 2505 Size: 1106 5201 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5314 809 Size: 507 3998 1802 Fragments arranged by size: 3998 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2973 3078 3306 Size: 54 1425 50 275 18 1143 105 228 Cuts at: 3306 3341 3754 4643 6272 8 Size: 35 413 889 1629 43 Fragments arranged by size: 1629 1425 1143 889 413 275 228 105 54 50 43 35 18 EagI C'GGCC_G Cuts at: 1537 1812 3754 1537 Size: 275 1942 4090 Fragments arranged by size: 4090 1942 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2400 2817 4237 1353 Size: 1047 417 1420 3423 Fragments arranged by size: 3423 1420 1047 417 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2174 2845 4894 5268 5414 387 Size: 1064 138 585 671 2049 374 146 1280 Fragments arranged by size: 2049 1280 1064 671 585 374 146 138 EcoICRI GAG'CTC Cuts at: 2215 2215 Size: 6307 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2302 4370 4967 5754 6022 1239 Size: 393 512 158 2068 597 787 268 1524 Fragments arranged by size: 2068 1524 787 597 512 393 268 158 EcoO109I rG'GnC_Cy Cuts at: 2617 2617 Size: 6307 EcoRI G'AATT_C Cuts at: 2660 3616 3994 2660 Size: 956 378 4973 Fragments arranged by size: 4973 956 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2671 3122 2671 Size: 451 5856 FseI GG_CCGG'CC Cuts at: 3432 3432 Size: 6307 FspI TGC'GCA Cuts at: 4813 4813 Size: 6307 HaeII r_GCGC'y Cuts at: 1678 3294 5454 1678 Size: 1616 2160 2531 Fragments arranged by size: 2531 2160 1616 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2903 2935 4948 Size: 32 135 32 773 32 526 32 2013 Cuts at: 4948 4980 5022 5054 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2013 773 526 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3480 3651 3897 669 Size: 988 281 1542 171 246 3079 Fragments arranged by size: 3079 1542 988 281 246 171 HindIII A'AGCT_T Cuts at: 748 3104 748 Size: 2356 3951 HpaI GTT'AAC Cuts at: 3897 3897 Size: 6307 KpnI G_GTAC'C Cuts at: 3539 3632 3539 Size: 93 6214 MfeI C'AATT_G Cuts at: 3906 3906 Size: 6307 MluI A'CGCG_T Cuts at: 6183 6183 Size: 6307 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2240 5421 5605 1305 Size: 935 3181 184 2007 Fragments arranged by size: 3181 2007 935 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2975 3080 3343 10 Size: 54 1425 343 1143 105 263 2974 Fragments arranged by size: 2974 1425 1143 343 263 105 54 MslI CAynn'nnrTG Cuts at: 518 1166 2570 2600 2885 4265 4624 4783 518 Size: 648 1404 30 285 1380 359 159 2042 Fragments arranged by size: 2042 1404 1380 648 359 285 159 30 MspA1I CmG'CkG Cuts at: 1606 3541 3723 4400 5548 5793 1606 Size: 1935 182 677 1148 245 2120 Fragments arranged by size: 2120 1935 1148 677 245 182 NaeI GCC'GGC Cuts at: 3430 3430 Size: 6307 NcoI C'CATG_G Cuts at: 513 1065 2049 2880 513 Size: 552 984 831 3940 Fragments arranged by size: 3940 984 831 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6307 NgoMIV G'CCGG_C Cuts at: 3428 3428 Size: 6307 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6307 NotI GC'GGCC_GC Cuts at: 3754 3754 Size: 6307 NspI r_CATG'y Cuts at: 1132 1229 3665 5210 1132 Size: 97 2436 1545 2229 Fragments arranged by size: 2436 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6307 PciI A'CATG_T Cuts at: 5206 5206 Size: 6307 PflMI CCAn_nnn'nTGG Cuts at: 1108 3578 1108 Size: 2470 3837 PfoI T'CCnGG_A Cuts at: 2853 2853 Size: 6307 PmeI GTTT'AAAC Cuts at: 3611 3611 Size: 6307 PmlI CAC'GTG Cuts at: 3493 3493 Size: 6307 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4376 4408 5889 5921 4376 Size: 32 1481 32 4762 Fragments arranged by size: 4762 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6307 PsiI TTA'TAA Cuts at: 3877 3877 Size: 6307 PspXI vC'TCGA_Gb Cuts at: 3164 3164 Size: 6307 PstI C_TGCA'G Cuts at: 830 3161 3546 3659 830 Size: 2331 385 113 3478 Fragments arranged by size: 3478 2331 385 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6307 SacI G_AGCT'C Cuts at: 2217 2217 Size: 6307 SalI G'TCGA_C Cuts at: 1936 3649 1936 Size: 1713 4594 SbfI CC_TGCA'GG Cuts at: 3659 3659 Size: 6307 ScaI AGT'ACT Cuts at: 4555 6267 4555 Size: 1712 4595 SfcI C'TryA_G Cuts at: 826 1045 3157 3542 3655 4790 5471 5662 826 Size: 219 2112 385 113 1135 681 191 1471 Fragments arranged by size: 2112 1471 1135 681 385 219 191 113 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6307 SmaI CCC'GGG Cuts at: 1872 3634 1872 Size: 1762 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2116 3164 3467 4411 5312 5574 Size: 197 926 173 1048 303 944 901 262 Cuts at: 5574 820 Size: 1553 Fragments arranged by size: 1553 1048 944 926 901 303 262 197 173 SnaBI TAC'GTA Cuts at: 493 4098 493 Size: 3605 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6307 SphI G_CATG'C Cuts at: 1132 3665 1132 Size: 2533 3774 SspI AAT'ATT Cuts at: 5 52 4231 5 Size: 47 4179 2081 Fragments arranged by size: 4179 2081 47 StuI AGG'CCT Cuts at: 1517 2136 2980 3011 3174 1517 Size: 619 844 31 163 4650 Fragments arranged by size: 4650 844 619 163 31 StyI C'CwwG_G Cuts at: 513 1065 2016 2049 2880 3565 3746 513 Size: 552 951 33 831 685 181 3074 Fragments arranged by size: 3074 951 831 685 552 181 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2919 3302 4334 4351 4504 4689 5134 Size: 1255 1339 383 1032 17 153 185 445 Cuts at: 5134 325 Size: 1498 Fragments arranged by size: 1498 1339 1255 1032 445 383 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4553 6265 96 Size: 274 80 33 51 1443 2576 1712 138 Fragments arranged by size: 2576 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6267 6299 6267 Size: 32 6275 Tth111I GACn'n_nGTC Cuts at: 1316 2903 1316 Size: 1587 4720 XbaI T'CTAG_A Cuts at: 3643 3643 Size: 6307 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2056 2680 3073 2056 Size: 624 393 5290 Fragments arranged by size: 5290 624 393 XhoI C'TCGA_G Cuts at: 1943 3164 1943 Size: 1221 5086 XmaI C'CCGG_G Cuts at: 1870 3632 1870 Size: 1762 4545 XmnI GAAnn'nnTTC Cuts at: 4436 4436 Size: 6307 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4115 276 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BbeI BfrBI BsiWI BstZ17I DraIII EcoNI EcoRV FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI