################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BcgI BfrI BlnI BsaI BseAI BsiEI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BsrDI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI Tth111I Van91I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccII 2306 CGCG CG^CG ApaI 2234 GGGCCC GGGCC^C AvaI 241 CTCGAG C^[CT]CG[GA]G BalI 2085 TGGCCA TGG^CCA BciVI 1762 GGATAC GTATCC(6/5) BglI 2078 GCCGTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 79 GCTGAGC GC^T[ACGT]AGC Bpu1102I 79 GCTGAGC GC^T[ACGT]AGC BsaBI 1740 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 499 ACCGGA [AT]^CCGG[AT] BsoBI 241 CTCGAG C^[CT]CG[GA]G BspMI 2222 ACCTGC ACCTGC(4/8) BsrFI 520 GCCGGT [GA]^CCGG[CT] BstBI 14 TTCGAA TT^CGAA BstUI 2306 CGCG CG^CG CelII 79 GCTGAGC GC^T[ACGT]AGC Cfr10I 520 GCCGGT [GA]^CCGG[CT] DrdI 2152 GACGTCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 2085 TGGCCA [CT]^GGCC[GA] EcoNI 849 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 261 CCCGC CCCGC(4/6) HindIII 357 AAGCTT A^AGCTT MamI 1740 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2085 TGGCCA TGG^CCA MscI 2085 TGGCCA TGG^CCA MvnI 2306 CGCG CG^CG NspV 14 TTCGAA TT^CGAA PaeR7I 241 CTCGAG C^TCGAG PshAI 1231 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 1433 GCTCTTC GCTCTTC(1/4) ScaI 2186 AGTACT AGT^ACT SexAI 1766 ACCTGGT A^CC[AT]GGT SfuI 14 TTCGAA TT^CGAA SmlI 241 CTCGAG C^T[CT][GA]AG SspI 1132 AATATT AAT^ATT StuI 344 AGGCCT AGG^CCT SwaI 116 ATTTAAAT ATTT^AAAT XbaI 297 TCTAGA T^CTAGA XhoI 241 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 736 GTCTAC GT^[AC][GT]AC 913 GTCTAC AfaI 591 GTAC GT^AC 2187 GTAC AlwNI 482 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG 844 CAGCGCCTG AvaII 1103 GGACC G^G[AT]CC 1515 GGACC BamHI 627 GGATCC G^GATCC 2024 GGATCC BbsI 205 GAAGAC GAAGAC(2/6) 1305 GAAGAC BbvCI 1671 CCTCAGC CCTCAGC(-5/-2) 2238 CCTCAGC BcnI 981 CCCGG CC^[GC]GG 1164 CCGGG BglII 710 AGATCT A^GATCT 971 AGATCT BpuAI 205 GAAGAC GAAGAC(2/6) 1305 GAAGAC BsaAI 998 CACGTA [CT]AC^GT[GA] 1810 CACGTA BspHI 1058 TCATGA T^CATGA 1775 TCATGA BspLU11I 929 ACATGT A^CATGT 1853 ACATGT BsrBI 262 CCGCTC CCGCTC(-3/-3) 556 GAGCGG BstAPI 481 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2409 GCACGCCCTGC DraI 117 TTTAAA TTT^AAA 2488 TTTAAA DraII 2234 GGGCCCT [GA]G^G[ACGT]CC[CT] 2390 AGGGCCT EcoO109I 2234 GGGCCCT [GA]G^G[ACGT]CC[CT] 2390 AGGGCCT HaeII 845 AGCGCC [GA]GCGC^[CT] 1343 GGCGCT MslI 888 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1848 CATCAACATG NciI 981 CCCGG CC^[GC]GG 1164 CCGGG NcoI 1046 CCATGG C^CATGG 2127 CCATGG NspI 929 ACATGT [GA]CATG^[CT] 1853 ACATGT PleI 1876 GACTC GAGTC(4/5) 832 GAGTC RcaI 1058 TCATGA T^CATGA 1775 TCATGA RsaI 591 GTAC GT^AC 2187 GTAC SacI 82 GAGCTC GAGCT^C 91 GAGCTC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AatII AciI AcsI AcyI AflIII AluI AlwI ApoI Asp700I AspHI BanII BbvI BclI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI