(Circular) (Six-base) MAPSORT of: pFN21AE6763.seq Check: 8004 from: 1 to: 7225 pFN21AE6763 7225 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2952 3813 4161 5035 Size: 53 83 186 936 1416 861 348 874 Cuts at: 5035 278 Size: 2468 Fragments arranged by size: 2468 1416 936 874 861 348 186 83 53 AccI GT'mk_AC Cuts at: 1937 2742 2919 4568 1937 Size: 805 177 1649 4594 Fragments arranged by size: 4594 1649 805 177 Acc65I G'GTAC_C Cuts at: 4546 4546 Size: 7225 AclI AA'CG_TT Cuts at: 1563 5352 5725 1563 Size: 3789 373 3063 Fragments arranged by size: 3789 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2417 3685 4289 4290 5288 6672 6940 1632 Size: 785 1268 604 1 998 1384 268 1917 Fragments arranged by size: 1917 1384 1268 998 785 604 268 1 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7028 AflIII A'CryG_T Cuts at: 1203 2592 2934 3002 3858 6124 7101 1203 Size: 1389 342 68 856 2266 977 1327 Fragments arranged by size: 2266 1389 1327 977 856 342 68 AgeI A'CCGG_T Cuts at: 6056 6056 Size: 7225 AhdI GACnn_n'nnGTC Cuts at: 5954 5954 Size: 7225 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7225 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7225 AlwNI CAG_nnn'CTG Cuts at: 2492 2854 4968 6540 2492 Size: 362 2114 1572 3177 Fragments arranged by size: 3177 2114 1572 362 ApaI G_GGCC'C Cuts at: 4243 4243 Size: 7225 ApaLI G'TGCA_C Cuts at: 5282 6438 5282 Size: 1156 6069 ApoI r'AATT_y Cuts at: 1493 2153 2652 3116 4534 4760 4912 7021 1493 Size: 660 499 464 1418 226 152 2109 1697 Fragments arranged by size: 2109 1697 1418 660 499 464 226 152 AseI AT'TA_AT Cuts at: 160 1032 5779 160 Size: 872 4747 1606 Fragments arranged by size: 4747 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7225 AvaI C'yCGr_G Cuts at: 1870 1943 2246 4550 1870 Size: 73 303 2304 4545 Fragments arranged by size: 4545 2304 303 73 BamHI G'GATC_C Cuts at: 2632 4029 4555 4922 2632 Size: 1397 526 367 4935 Fragments arranged by size: 4935 1397 526 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4546 6001 618 Size: 325 217 627 2759 1455 1842 Fragments arranged by size: 2759 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2091 2100 4123 4243 4544 1391 Size: 700 9 2023 120 301 4072 Fragments arranged by size: 4072 2023 700 301 120 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2217 3317 928 Size: 971 318 1100 4836 Fragments arranged by size: 4836 1100 971 318 BbvCI CC'TCA_GC Cuts at: 3677 4244 3677 Size: 567 6658 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5416 5450 1903 Size: 34 3479 34 3678 Fragments arranged by size: 3678 3479 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3761 5117 6327 3761 Size: 1356 1210 4659 Fragments arranged by size: 4659 1356 1210 BclI T'GATC_A Cuts at: 1548 2532 3783 4386 1548 Size: 984 1251 603 4387 Fragments arranged by size: 4387 1251 984 603 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4089 5836 136 Size: 107 122 71 631 605 2417 1747 1525 Fragments arranged by size: 2417 1747 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2715 2976 7220 2715 Size: 261 4244 2720 Fragments arranged by size: 4244 2720 261 BlpI GC'TnA_GC Cuts at: 2085 4642 2085 Size: 2557 4668 Bme1580I G_kGCm'C Cuts at: 1790 3019 4243 5286 6442 1790 Size: 1229 1224 1043 1156 2573 Fragments arranged by size: 2573 1229 1224 1156 1043 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7225 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4441 4956 5914 Size: 416 53 83 399 24 3024 515 958 Cuts at: 5914 442 Size: 1753 Fragments arranged by size: 3024 1753 958 515 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7225 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2711 4417 2711 Size: 1706 5519 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 2207 2312 2495 2648 3041 4054 5885 Size: 800 168 105 183 153 393 1013 1831 Cuts at: 5885 1239 Size: 2579 Fragments arranged by size: 2579 1831 1013 800 393 183 168 153 105 Bpu10I CC'TnA_GC Cuts at: 2696 3677 4244 2696 Size: 981 567 5677 Fragments arranged by size: 5677 981 567 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5350 6215 6513 5350 Size: 865 298 6062 Fragments arranged by size: 6062 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5888 882 Size: 617 4389 2219 Fragments arranged by size: 4389 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3005 3817 5016 493 Size: 711 1801 812 1199 2702 Fragments arranged by size: 2702 1801 1199 812 711 BsaBI GATnn'nnATC Cuts at: 3749 7219 3749 Size: 3470 3755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2619 2949 3810 4158 Size: 53 83 186 936 1086 330 861 348 Cuts at: 4158 5032 5414 6049 275 Size: 874 382 635 1451 Fragments arranged by size: 1451 1086 936 874 861 635 382 348 330 186 83 53 BsaWI w'CCGG_w Cuts at: 2504 5658 6056 6330 6477 6888 2504 Size: 3154 398 274 147 411 2841 Fragments arranged by size: 3154 2841 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2037 2067 2867 2897 3076 Size: 30 165 30 634 30 800 30 179 Cuts at: 3076 3106 3398 3428 4205 4235 4314 4344 1178 Size: 30 292 30 777 30 79 30 4059 Fragments arranged by size: 4059 800 777 634 292 179 165 79 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 3037 4535 1186 Size: 856 995 1498 3876 Fragments arranged by size: 3876 1498 995 856 BseYI C'CCAG_C Cuts at: 2955 3181 3523 3833 4011 4317 6428 6924 2955 Size: 226 342 310 178 306 2111 496 3256 Fragments arranged by size: 3256 2111 496 342 310 306 226 178 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2921 3252 4397 4412 1163 Size: 573 1185 331 1145 15 3976 Fragments arranged by size: 3976 1185 1145 573 331 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4675 5436 6464 1540 Size: 275 186 2674 761 1028 2301 Fragments arranged by size: 2674 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2091 2100 4278 5286 5371 6442 2091 Size: 9 2178 1008 85 1071 2874 Fragments arranged by size: 2874 2178 1071 1008 85 9 BsmI GAATG_Cn' Cuts at: 4736 4829 4736 Size: 93 7132 BsmBI CGTCTCn'nnnn_ Cuts at: 2414 3286 4149 2414 Size: 872 863 5490 Fragments arranged by size: 5490 872 863 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2100 3019 4123 4243 4278 4544 Size: 399 301 9 919 1104 120 35 266 Cuts at: 4544 5286 5371 6442 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1104 1071 919 742 399 301 266 120 85 35 9 BspEI T'CCGG_A Cuts at: 6888 6888 Size: 7225 BspHI T'CATG_A Cuts at: 3063 3780 5114 6844 3063 Size: 717 1334 1730 3444 Fragments arranged by size: 3444 1730 1334 717 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4236 4580 844 Size: 3392 344 3489 Fragments arranged by size: 3489 3392 344 BsrBI CCG'CTC Cuts at: 1394 1823 2269 2563 5112 1394 Size: 429 446 294 2549 3507 Fragments arranged by size: 3507 2549 446 429 294 BsrDI GCAATG_nn' Cuts at: 65 1248 5720 5894 65 Size: 1183 4472 174 1396 Fragments arranged by size: 4472 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2525 5869 6056 1700 Size: 110 715 3344 187 2869 Fragments arranged by size: 3344 2869 715 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7225 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7225 BssSI C'ACGA_G Cuts at: 5285 6297 5285 Size: 1012 6213 BstAPI GCAn_nnn'nTGC Cuts at: 794 2492 4420 794 Size: 1698 1928 3599 Fragments arranged by size: 3599 1928 1698 BstBI TT'CG_AA Cuts at: 2020 6029 2020 Size: 4009 3216 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7225 BstYI r'GATC_y Cuts at: 1972 2632 2715 2976 4029 4555 4922 5307 5324 Size: 660 83 261 1053 526 367 385 17 Cuts at: 5324 6776 6862 6874 7220 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1053 660 526 385 367 346 261 86 83 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2273 3051 3343 3959 4114 4132 Size: 552 939 269 778 292 616 155 18 Cuts at: 4132 513 Size: 3606 Fragments arranged by size: 3606 939 778 616 552 292 269 155 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2953 487 Size: 683 56 1727 4759 Fragments arranged by size: 4759 1727 683 56 BtsI GCAGTG_nn' Cuts at: 2493 2966 4741 5535 5555 2493 Size: 473 1775 794 20 4163 Fragments arranged by size: 4163 1775 794 473 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7225 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7190 DraI TTT'AAA Cuts at: 2124 4495 4529 2124 Size: 2371 34 4820 Fragments arranged by size: 4820 2371 34 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4163 6232 809 Size: 507 2847 2069 1802 Fragments arranged by size: 2847 2069 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4090 4672 5561 Size: 54 1425 50 275 18 2260 582 889 Cuts at: 5561 7190 8 Size: 1629 43 Fragments arranged by size: 2260 1629 1425 889 582 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4672 1537 Size: 275 2860 4090 Fragments arranged by size: 4090 2860 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3445 3889 4134 5155 1353 Size: 2092 444 245 1021 3423 Fragments arranged by size: 3423 2092 1021 444 245 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5812 6186 6332 387 Size: 1064 138 4223 374 146 1280 Fragments arranged by size: 4223 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2089 2098 2089 Size: 9 7216 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2207 2312 2417 2495 2648 3041 Size: 393 407 168 105 105 78 153 393 Cuts at: 3041 3685 4054 4289 4290 5288 5885 6672 6940 Size: 644 369 235 1 998 597 787 268 Cuts at: 6940 1239 Size: 1524 Fragments arranged by size: 1524 998 787 644 597 407 393 393 369 268 235 168 153 105 105 78 1 EcoNI CCTnn'n_nnAGG Cuts at: 2858 2858 Size: 7225 EcoO109I rG'GnC_Cy Cuts at: 4240 4396 4240 Size: 156 7069 EcoRI G'AATT_C Cuts at: 4534 4912 4534 Size: 378 6847 FspI TGC'GCA Cuts at: 5731 5731 Size: 7225 HaeII r_GCGC'y Cuts at: 1678 2854 3352 6372 1678 Size: 1176 498 3020 2531 Fragments arranged by size: 3020 2531 1176 498 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2724 2756 3285 3317 3398 Size: 32 135 32 1152 32 529 32 81 Cuts at: 3398 3430 4314 4346 5866 5898 5940 5972 1373 Size: 32 884 32 1520 32 42 32 2626 Fragments arranged by size: 2626 1520 1152 884 529 135 81 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4569 4815 669 Size: 988 281 2631 246 3079 Fragments arranged by size: 3079 2631 988 281 246 HindIII A'AGCT_T Cuts at: 748 2362 748 Size: 1614 5611 HpaI GTT'AAC Cuts at: 4815 4815 Size: 7225 KpnI G_GTAC'C Cuts at: 4550 4550 Size: 7225 MfeI C'AATT_G Cuts at: 4824 4824 Size: 7225 MluI A'CGCG_T Cuts at: 7101 7101 Size: 7225 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2763 3478 3912 6339 6523 1305 Size: 1458 715 434 2427 184 2007 Fragments arranged by size: 2427 2007 1458 715 434 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4092 10 Size: 54 1425 343 2260 3143 Fragments arranged by size: 3143 2260 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2897 3857 5183 5542 5701 518 Size: 648 1731 960 1326 359 159 2042 Fragments arranged by size: 2042 1731 1326 960 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2870 3125 3517 4015 4299 4641 5318 6466 Size: 1264 255 392 498 284 342 677 1148 Cuts at: 6466 6711 1606 Size: 245 2120 Fragments arranged by size: 2120 1264 1148 677 498 392 342 284 255 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3051 4132 513 Size: 552 939 1047 1081 3606 Fragments arranged by size: 3606 1081 1047 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7225 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7225 NotI GC'GGCC_GC Cuts at: 4672 4672 Size: 7225 NspI r_CATG'y Cuts at: 1132 1229 2938 3862 4583 6128 1132 Size: 97 1709 924 721 1545 2229 Fragments arranged by size: 2229 1709 1545 924 721 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7225 PciI A'CATG_T Cuts at: 2934 3858 6124 2934 Size: 924 2266 4035 Fragments arranged by size: 4035 2266 924 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7225 PfoI T'CCnGG_A Cuts at: 2472 4025 4503 2472 Size: 1553 478 5194 Fragments arranged by size: 5194 1553 478 PmeI GTTT'AAAC Cuts at: 4529 4529 Size: 7225 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2966 2998 5294 5326 6807 6839 2966 Size: 32 2296 32 1481 32 3352 Fragments arranged by size: 3352 2296 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3240 1613 Size: 1627 5598 PsiI TTA'TAA Cuts at: 4795 4795 Size: 7225 PspOMI G'GGCC_C Cuts at: 4239 4239 Size: 7225 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2588 2620 2588 Size: 32 7193 PstI C_TGCA'G Cuts at: 830 2862 3516 4020 4108 4577 830 Size: 2032 654 504 88 469 3478 Fragments arranged by size: 3478 2032 654 504 469 88 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7225 PvuII CAG'CTG Cuts at: 3125 3517 4015 3125 Size: 392 498 6335 Fragments arranged by size: 6335 498 392 SacI G_AGCT'C Cuts at: 2091 2100 2091 Size: 9 7216 SalI G'TCGA_C Cuts at: 1936 4567 1936 Size: 2631 4594 SapI GCTCTTCn'nnn_ Cuts at: 3445 3445 Size: 7225 SbfI CC_TGCA'GG Cuts at: 4577 4577 Size: 7225 ScaI AGT'ACT Cuts at: 4193 5473 7185 4193 Size: 1280 1712 4233 Fragments arranged by size: 4233 1712 1280 SexAI A'CCwGG_T Cuts at: 3771 3771 Size: 7225 SfcI C'TryA_G Cuts at: 826 1045 2858 3512 3874 4016 4104 4573 5708 Size: 219 1813 654 362 142 88 469 1135 Cuts at: 5708 6389 6580 826 Size: 681 191 1471 Fragments arranged by size: 1813 1471 1135 681 654 469 362 219 191 142 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7225 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7225 SmaI CCC'GGG Cuts at: 1872 4552 1872 Size: 2680 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2246 5329 6230 6492 820 Size: 197 926 303 3083 901 262 1553 Fragments arranged by size: 3083 1553 926 901 303 262 197 SnaBI TAC'GTA Cuts at: 493 5016 493 Size: 4523 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7225 SphI G_CATG'C Cuts at: 1132 4583 1132 Size: 3451 3774 SspI AAT'ATT Cuts at: 5 52 3139 5149 5 Size: 47 3087 2010 2081 Fragments arranged by size: 3087 2081 2010 47 StuI AGG'CCT Cuts at: 1517 2351 1517 Size: 834 6391 StyI C'CwwG_G Cuts at: 513 1065 2004 2683 3051 4132 4664 513 Size: 552 939 679 368 1081 532 3074 Fragments arranged by size: 3074 1081 939 679 552 532 368 SwaI ATTT'AAAT Cuts at: 2124 2124 Size: 7225 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2969 5252 5269 5422 5607 6052 325 Size: 1255 1389 2283 17 153 185 445 1498 Fragments arranged by size: 2283 1498 1389 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2595 4191 5471 Size: 274 80 33 51 1443 618 1596 1280 Cuts at: 5471 7183 96 Size: 1712 138 Fragments arranged by size: 1712 1596 1443 1280 618 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7185 7217 7185 Size: 32 7193 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7225 XbaI T'CTAG_A Cuts at: 2302 4561 2302 Size: 2259 4966 XhoI C'TCGA_G Cuts at: 1943 2246 1943 Size: 303 6922 XmaI C'CCGG_G Cuts at: 1870 4550 1870 Size: 2680 4545 XmnI GAAnn'nnTTC Cuts at: 2652 2666 3335 4451 5354 2652 Size: 14 669 1116 903 4523 Fragments arranged by size: 4523 1116 903 669 14 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2950 3811 4159 5033 Size: 53 83 186 936 1416 861 348 874 Cuts at: 5033 276 Size: 2468 Fragments arranged by size: 2468 1416 936 874 861 348 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI RsrII SacII SanDI SfiI SfoI SrfI XcmI