(Linear) (Six-base) MAPSORT of: KIEE6763.seq Check: 6846 from: 1 to: 2520 KIEE6763.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AatII G_ACGT'C Cuts at: 0 947 1808 2156 2520 Size: 947 861 348 364 Fragments arranged by size: 947 861 364 348 AccI GT'mk_AC Cuts at: 0 737 914 2520 Size: 737 177 1606 Fragments arranged by size: 1606 737 177 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 1680 2284 2285 2520 Size: 412 1268 604 1 235 Fragments arranged by size: 1268 604 412 235 1 AflIII A'CryG_T Cuts at: 0 587 929 997 1853 2520 Size: 587 342 68 856 667 Fragments arranged by size: 856 667 587 342 68 AlwNI CAG_nnn'CTG Cuts at: 0 487 849 2520 Size: 487 362 1671 Fragments arranged by size: 1671 487 362 ApaI G_GGCC'C Cuts at: 0 2238 2520 Size: 2238 282 ApoI r'AATT_y Cuts at: 0 148 647 1111 2520 Size: 148 499 464 1409 Fragments arranged by size: 1409 499 464 148 AvaI C'yCGr_G Cuts at: 0 241 2520 Size: 241 2279 BamHI G'GATC_C Cuts at: 0 627 2024 2520 Size: 627 1397 496 Fragments arranged by size: 1397 627 496 BanII G_rGCy'C Cuts at: 0 86 95 2118 2238 2520 Size: 86 9 2023 120 282 Fragments arranged by size: 2023 282 120 86 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 212 1312 2520 Size: 212 1100 1208 Fragments arranged by size: 1208 1100 212 BbvCI CC'TCA_GC Cuts at: 0 1672 2239 2520 Size: 1672 567 281 Fragments arranged by size: 1672 567 281 BciVI GTATCCnnnnn_n' Cuts at: 0 1756 2520 Size: 1756 764 BclI T'GATC_A Cuts at: 0 527 1778 2381 2520 Size: 527 1251 603 139 Fragments arranged by size: 1251 603 527 139 BglI GCCn_nnn'nGGC Cuts at: 0 2084 2520 Size: 2084 436 BglII A'GATC_T Cuts at: 0 710 971 2520 Size: 710 261 1549 Fragments arranged by size: 1549 710 261 BlpI GC'TnA_GC Cuts at: 0 80 2520 Size: 80 2440 Bme1580I G_kGCm'C Cuts at: 0 1014 2238 2520 Size: 1014 1224 282 Fragments arranged by size: 1224 1014 282 BmrI ACTGGGnnnn_n' Cuts at: 0 2436 2520 Size: 2436 84 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 706 2412 2520 Size: 706 1706 108 Fragments arranged by size: 1706 706 108 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 202 307 490 643 1036 2049 2520 Size: 34 168 105 183 153 393 1013 471 Fragments arranged by size: 1013 471 393 183 168 153 105 34 Bpu10I CC'TnA_GC Cuts at: 0 691 1672 2239 2520 Size: 691 981 567 281 Fragments arranged by size: 981 691 567 281 BsaAI yAC'GTr Cuts at: 0 1000 1812 2520 Size: 1000 812 708 Fragments arranged by size: 1000 812 708 BsaBI GATnn'nnATC Cuts at: 0 1744 2520 Size: 1744 776 BsaHI Gr'CG_yC Cuts at: 0 614 944 1805 2153 2520 Size: 614 330 861 348 367 Fragments arranged by size: 861 614 367 348 330 BsaWI w'CCGG_w Cuts at: 0 499 2520 Size: 499 2021 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 32 62 862 892 1071 1101 1393 1423 Size: 32 30 800 30 179 30 292 30 Cuts at: 1423 2200 2230 2309 2339 2520 Size: 777 30 79 30 181 Fragments arranged by size: 800 777 292 181 179 79 32 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 37 1032 2520 Size: 37 995 1488 Fragments arranged by size: 1488 995 37 BseYI C'CCAG_C Cuts at: 0 950 1176 1518 1828 2006 2312 2520 Size: 950 226 342 310 178 306 208 Fragments arranged by size: 950 342 310 306 226 208 178 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 916 1247 2392 2407 2520 Size: 916 331 1145 15 113 Fragments arranged by size: 1145 916 331 113 15 BsiHKAI G_wGCw'C Cuts at: 0 86 95 2273 2520 Size: 86 9 2178 247 Fragments arranged by size: 2178 247 86 9 BsmBI CGTCTCn'nnnn_ Cuts at: 0 409 1281 2144 2520 Size: 409 872 863 376 Fragments arranged by size: 872 863 409 376 Bsp1286I G_dGCh'C Cuts at: 0 86 95 1014 2118 2238 2273 2520 Size: 86 9 919 1104 120 35 247 Fragments arranged by size: 1104 919 247 120 86 35 9 BspHI T'CATG_A Cuts at: 0 1058 1775 2520 Size: 1058 717 745 Fragments arranged by size: 1058 745 717 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2231 2520 Size: 2231 289 BsrBI CCG'CTC Cuts at: 0 264 558 2520 Size: 264 294 1962 Fragments arranged by size: 1962 294 264 BsrFI r'CCGG_y Cuts at: 0 520 2520 Size: 520 2000 BstAPI GCAn_nnn'nTGC Cuts at: 0 487 2415 2520 Size: 487 1928 105 Fragments arranged by size: 1928 487 105 BstBI TT'CG_AA Cuts at: 0 15 2520 Size: 15 2505 BstYI r'GATC_y Cuts at: 0 627 710 971 2024 2520 Size: 627 83 261 1053 496 Fragments arranged by size: 1053 627 496 261 83 BtgI C'CryG_G Cuts at: 0 268 1046 1338 1954 2109 2127 2520 Size: 268 778 292 616 155 18 393 Fragments arranged by size: 778 616 393 292 268 155 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 948 2520 Size: 948 1572 BtsI GCAGTG_nn' Cuts at: 0 488 961 2520 Size: 488 473 1559 Fragments arranged by size: 1559 488 473 DraI TTT'AAA Cuts at: 0 119 2490 2520 Size: 119 2371 30 Fragments arranged by size: 2371 119 30 DrdI GACnn_nn'nnGTC Cuts at: 0 2158 2520 Size: 2158 362 EaeI y'GGCC_r Cuts at: 0 2085 2520 Size: 2085 435 EarI CTCTTCn'nnn_ Cuts at: 0 1440 1884 2129 2520 Size: 1440 444 245 391 Fragments arranged by size: 1440 444 391 245 EcoICRI GAG'CTC Cuts at: 0 84 93 2520 Size: 84 9 2427 Fragments arranged by size: 2427 84 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 202 307 412 490 643 1036 1680 Size: 34 168 105 105 78 153 393 644 Cuts at: 1680 2049 2284 2285 2520 Size: 369 235 1 235 Fragments arranged by size: 644 393 369 235 235 168 153 105 105 78 34 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 853 2520 Size: 853 1667 EcoO109I rG'GnC_Cy Cuts at: 0 2235 2391 2520 Size: 2235 156 129 Fragments arranged by size: 2235 156 129 HaeII r_GCGC'y Cuts at: 0 849 1347 2520 Size: 849 498 1173 Fragments arranged by size: 1173 849 498 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 719 751 1280 1312 1393 1425 2309 2341 Size: 719 32 529 32 81 32 884 32 Cuts at: 2341 2520 Size: 179 Fragments arranged by size: 884 719 529 179 81 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 357 2520 Size: 357 2163 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 758 1473 1907 2520 Size: 758 715 434 613 Fragments arranged by size: 758 715 613 434 MscI TGG'CCA Cuts at: 0 2087 2520 Size: 2087 433 MslI CAynn'nnrTG Cuts at: 0 892 1852 2520 Size: 892 960 668 Fragments arranged by size: 960 892 668 MspA1I CmG'CkG Cuts at: 0 865 1120 1512 2010 2294 2520 Size: 865 255 392 498 284 226 Fragments arranged by size: 865 498 392 284 255 226 NcoI C'CATG_G Cuts at: 0 1046 2127 2520 Size: 1046 1081 393 Fragments arranged by size: 1081 1046 393 NspI r_CATG'y Cuts at: 0 933 1857 2520 Size: 933 924 663 Fragments arranged by size: 933 924 663 PciI A'CATG_T Cuts at: 0 929 1853 2520 Size: 929 924 667 Fragments arranged by size: 929 924 667 PfoI T'CCnGG_A Cuts at: 0 467 2020 2498 2520 Size: 467 1553 478 22 Fragments arranged by size: 1553 478 467 22 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 961 993 2520 Size: 961 32 1527 Fragments arranged by size: 1527 961 32 PshAI GACnn'nnGTC Cuts at: 0 1235 2520 Size: 1235 1285 PspOMI G'GGCC_C Cuts at: 0 2234 2520 Size: 2234 286 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 583 615 2520 Size: 583 32 1905 Fragments arranged by size: 1905 583 32 PstI C_TGCA'G Cuts at: 0 857 1511 2015 2103 2520 Size: 857 654 504 88 417 Fragments arranged by size: 857 654 504 417 88 PvuII CAG'CTG Cuts at: 0 1120 1512 2010 2520 Size: 1120 392 498 510 Fragments arranged by size: 1120 510 498 392 SacI G_AGCT'C Cuts at: 0 86 95 2520 Size: 86 9 2425 Fragments arranged by size: 2425 86 9 SapI GCTCTTCn'nnn_ Cuts at: 0 1440 2520 Size: 1440 1080 ScaI AGT'ACT Cuts at: 0 2188 2520 Size: 2188 332 SexAI A'CCwGG_T Cuts at: 0 1766 2520 Size: 1766 754 SfcI C'TryA_G Cuts at: 0 853 1507 1869 2011 2099 2520 Size: 853 654 362 142 88 421 Fragments arranged by size: 853 654 421 362 142 88 SmlI C'TyrA_G Cuts at: 0 241 2520 Size: 241 2279 SspI AAT'ATT Cuts at: 0 1134 2520 Size: 1134 1386 StuI AGG'CCT Cuts at: 0 346 2520 Size: 346 2174 StyI C'CwwG_G Cuts at: 0 678 1046 2127 2520 Size: 678 368 1081 393 Fragments arranged by size: 1081 678 393 368 SwaI ATTT'AAAT Cuts at: 0 119 2520 Size: 119 2401 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 964 2520 Size: 964 1556 TatI w'GTAC_w Cuts at: 0 590 2186 2520 Size: 590 1596 334 Fragments arranged by size: 1596 590 334 XbaI T'CTAG_A Cuts at: 0 297 2520 Size: 297 2223 XhoI C'TCGA_G Cuts at: 0 241 2520 Size: 241 2279 XmnI GAAnn'nnTTC Cuts at: 0 647 661 1330 2446 2520 Size: 647 14 669 1116 74 Fragments arranged by size: 1116 669 647 74 14 ZraI GAC'GTC Cuts at: 0 945 1806 2154 2520 Size: 945 861 348 366 Fragments arranged by size: 945 861 366 348 Enzymes that do cut: AatII AccI AcuI AflIII AlwNI ApaI ApoI AvaI BamHI BanII BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI NspI PciI PfoI PpiI PshAI PspOMI PsrI PstI PvuII SacI SapI ScaI SexAI SfcI SmlI SspI StuI StyI SwaI TaqII TatI XbaI XhoI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI BcgI BfrBI BmgBI BmtI BpuEI BsaI BsiEI BsiWI BsmI BspEI BsrDI BsrGI BssHII BssSI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpuMI PsiI PspXI PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI TstI Tth111I XcmI XmaI