################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HaeII HapII Hin1I HincII HindII HpaI HpaII KasI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 943 CGCG CG^CG AocI 97 CCTAAGG CC^T[ACGT]AGG Asp700I 844 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 529 GAGCTC G[AT]GC[AT]^C AspI 637 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 529 GAGCTC G[GA]GC[CT]^C BglII 854 AGATCT A^GATCT BmyI 529 GAGCTC G[AGT]GC[ACT]^C BsiHKAI 529 GAGCTC G[AT]GC[AT]^C BsmI 50 GCATTC GAATGC(1/-1) Bsp1286I 529 GAGCTC G[AGT]GC[ACT]^C BspHI 966 TCATGA T^CATGA BstUI 943 CGCG CG^CG Bsu36I 97 CCTAAGG CC^T[ACGT]AGG CfoI 942 GCGC GCG^C DsaI 365 CCATGG C^C[GA][CT]GG Eco81I 97 CCTAAGG CC^T[ACGT]AGG EcoRV 241 GATATC GAT^ATC EcoT14I 365 CCATGG C^C[AT][AT]GG HgaI 475 GACGC GACGC(5/10) HhaI 942 GCGC GCG^C HinP1I 942 GCGC G^CGC MaeIII 20 GTCAC ^GT[ACGT]AC MslI 151 CATATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 523 CAGCTG C[AC]G^C[GT]G MvnI 943 CGCG CG^CG NcoI 365 CCATGG C^CATGG PflFI 637 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 551 GACTC GAGTC(4/5) PpuMI 320 GGGTCCT [GA]G^G[AT]CC[CT] PvuII 523 CAGCTG CAG^CTG RcaI 966 TCATGA T^CATGA SacI 529 GAGCTC GAGCT^C SexAI 575 ACCAGGT A^CC[AT]GGT SfaNI 721 GATGC GCATC(5/9) StyI 365 CCATGG C^C[AT][AT]GG TaqI 91 TCGA T^CGA TfiI 678 GAATC G^A[AT]TC Tsp45I 20 GTCAC ^GT[GC]AC Tth111I 637 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 91 TCGA T^CGA XmnI 844 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### Acc65I 70 GGTACC G^GTACC 143 GGTACC AcsI 619 AAATTT [GA]^AATT[CT] 846 AAATTT ApoI 619 AAATTT [GA]^AATT[CT] 846 AAATTT Asp718I 70 GGTACC G^GTACC 143 GGTACC BanI 70 GGTACC G^G[CT][GA]CC 143 GGTACC BfaI 331 CTAG C^TAG 398 CTAG BsmFI 667 GGGAC GGGAC(10/14) 280 GTCCC BsrI 565 CCAGT ACTGG(1/-1) 671 CCAGT DraII 160 GGGGCCT [GA]G^G[ACGT]CC[CT] 320 GGGTCCT EcoO109I 160 GGGGCCT [GA]G^G[ACGT]CC[CT] 320 GGGTCCT HindIII 464 AAGCTT A^AGCTT 819 AAGCTT HinfI 551 GACTC G^A[ACGT]TC 678 GAATC KpnI 70 GGTACC GGTAC^C 143 GGTACC MaeI 331 CTAG C^TAG 398 CTAG TspRI 9 CAGTG CA[GC]TG[ACGT][ACGT]^ 873 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbvI BpmI BsaJI BsiYI BslI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HphI ItaI MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI Tru9I TseI Tsp509I XhoII