Restriction map for
pFN21AE1892
(Circular) (Six-base) MAPSORT of: pFN21AE1892.seq Check: 7430 from: 1 to: 5968
pFN21AE1892 5968 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3077 3077
Size: 5968
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3778 278
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2693 3311 1937
Size: 756 618 4594
Fragments arranged by size:
4594 756 618
Acc65I G'GTAC_C
Cuts at: 3289 3289
Size: 5968
AclI AA'CG_TT
Cuts at: 1563 4095 4468 1563
Size: 2532 373 3063
Fragments arranged by size:
3063 2532 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2507 3251 4031 5415 5683 1632
Size: 875 744 780 1384 268 1917
Fragments arranged by size:
1917 1384 875 780 744 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5771
AflIII A'CryG_T
Cuts at: 1203 4867 5844 1203
Size: 3664 977 1327
Fragments arranged by size:
3664 1327 977
AgeI A'CCGG_T
Cuts at: 4799 4799
Size: 5968
AhdI GACnn_n'nnGTC
Cuts at: 4697 4697
Size: 5968
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5968
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5968
AlwNI CAG_nnn'CTG
Cuts at: 2049 2944 3711 5283 2049
Size: 895 767 1572 2734
Fragments arranged by size:
2734 1572 895 767
ApaI G_GGCC'C
Cuts at: 2032 2032
Size: 5968
ApaLI G'TGCA_C
Cuts at: 4025 5181 4025
Size: 1156 4812
ApoI r'AATT_y
Cuts at: 1493 2063 2137 2902 3131 3277 3503 3655 5764
Size: 570 74 765 229 146 226 152 2109
Cuts at: 5764 1493
Size: 1697
Fragments arranged by size:
2109 1697 765 570 229 226 152 146
74
AseI AT'TA_AT
Cuts at: 160 1032 2091 4522 160
Size: 872 1059 2431 1606
Fragments arranged by size:
2431 1606 1059 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
AvaI C'yCGr_G
Cuts at: 1870 1943 2550 3293 1870
Size: 73 607 743 4545
Fragments arranged by size:
4545 743 607 73
BamHI G'GATC_C
Cuts at: 2617 3298 3665 2617
Size: 681 367 4920
Fragments arranged by size:
4920 681 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3289 4744 618
Size: 325 217 627 1502 1455 1842
Fragments arranged by size:
1842 1502 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2032 3287 1391
Size: 641 1255 4072
Fragments arranged by size:
4072 1255 641
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2729 3239 928
Size: 971 830 510 3657
Fragments arranged by size:
3657 971 830 510
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4159 4193 1903
Size: 34 2222 34 3678
Fragments arranged by size:
3678 2222 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2007 2961 3860 5070 2007
Size: 954 899 1210 2905
Fragments arranged by size:
2905 1210 954 899
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5968
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4579 136
Size: 107 122 71 631 605 2907 1525
Fragments arranged by size:
2907 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5963 5963
Size: 5968
BlpI GC'TnA_GC
Cuts at: 3385 3385
Size: 5968
Bme1580I G_kGCm'C
Cuts at: 1790 2032 3047 4029 5185 1790
Size: 242 1015 982 1156 2573
Fragments arranged by size:
2573 1156 1015 982 242
BmgBI CAC'GTC
Cuts at: 1318 3219 1318
Size: 1901 4067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2508 3118 3699
Size: 416 53 83 399 24 1091 610 581
Cuts at: 3699 4657 442
Size: 958 1753
Fragments arranged by size:
1753 1091 958 610 581 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3093 1056
Size: 2037 3931
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 5968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4628 1239
Size: 3389 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4093 4958 5256 4093
Size: 865 298 4805
Fragments arranged by size:
4805 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4631 882
Size: 617 3132 2219
Fragments arranged by size:
3132 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3759 493
Size: 711 2555 2702
Fragments arranged by size:
2702 2555 711
BsaBI GATnn'nnATC
Cuts at: 5962 5962
Size: 5968
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3775 4157 4792 275
Size: 53 83 186 936 2242 382 635 1451
Fragments arranged by size:
2242 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4401 4799 5073 5220 5631 4401
Size: 398 274 147 411 4738
Fragments arranged by size:
4738 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2807 2837 1178
Size: 30 165 30 1404 30 4309
Fragments arranged by size:
4309 1404 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2167 2452 3227 1186
Size: 981 285 775 3927
Fragments arranged by size:
3927 981 775 285
BseYI C'CCAG_C
Cuts at: 3223 5171 5667 3223
Size: 1948 496 3524
Fragments arranged by size:
3524 1948 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2909 3055 1163
Size: 573 1173 146 4076
Fragments arranged by size:
4076 1173 573 146
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2474 2519 2523 3418 4179 5207
Size: 275 186 473 45 4 895 761 1028
Cuts at: 5207 1540
Size: 2301
Fragments arranged by size:
2301 1028 895 761 473 275 186 45
4
BsiHKAI G_wGCw'C
Cuts at: 4029 4114 5185 4029
Size: 85 1071 4812
Fragments arranged by size:
4812 1071 85
BsmI GAATG_Cn'
Cuts at: 2539 3137 3479 3572 2539
Size: 598 342 93 4935
Fragments arranged by size:
4935 598 342 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2100 3209 2100
Size: 1109 4859
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 3047 3287 4029 4114 5185 1391
Size: 399 242 1015 240 742 85 1071 2174
Fragments arranged by size:
2174 1071 1015 742 399 242 240 85
BspEI T'CCGG_A
Cuts at: 5631 5631
Size: 5968
BspHI T'CATG_A
Cuts at: 3857 5587 3857
Size: 1730 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2292 2931 3077 3323 844
Size: 1448 639 146 246 3489
Fragments arranged by size:
3489 1448 639 246 146
BsrBI CCG'CTC
Cuts at: 1394 1823 3855 1394
Size: 429 2032 3507
Fragments arranged by size:
3507 2032 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2409 4463 4637 65
Size: 1183 1161 2054 174 1396
Fragments arranged by size:
2054 1396 1183 1161 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2469 2518 4612 4799 1700
Size: 110 659 49 2094 187 2869
Fragments arranged by size:
2869 2094 659 187 110 49
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5968
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5968
BssSI C'ACGA_G
Cuts at: 2396 4028 5040 2396
Size: 1632 1012 3324
Fragments arranged by size:
3324 1632 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5968
BstBI TT'CG_AA
Cuts at: 4772 4772
Size: 5968
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5968
BstXI CCAn_nnnn'nTGG
Cuts at: 2897 3028 2897
Size: 131 5837
BstYI r'GATC_y
Cuts at: 1972 2246 2559 2617 3298 3665 4050 4067 5519
Size: 274 313 58 681 367 385 17 1452
Cuts at: 5519 5605 5617 5963 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 681 385 367 346 313 274
86 58 17 12
BstZ17I GTA'TAC
Cuts at: 2694 2694
Size: 5968
BtgI C'CryG_G
Cuts at: 513 1065 2836 513
Size: 552 1771 3645
Fragments arranged by size:
3645 1771 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5229
Fragments arranged by size:
5229 683 56
BtsI GCAGTG_nn'
Cuts at: 2274 2571 3204 3484 4278 4298 2274
Size: 297 633 280 794 20 3944
Fragments arranged by size:
3944 794 633 297 280 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5968
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5933
DraI TTT'AAA
Cuts at: 2233 3272 2233
Size: 1039 4929
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4975 809
Size: 507 3659 1802
Fragments arranged by size:
3659 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 2471 2516
Size: 54 1425 50 275 18 223 418 45
Cuts at: 2516 2520 2670 3018 3415 4304 5933 8
Size: 4 150 348 397 889 1629 43
Fragments arranged by size:
1629 1425 889 418 397 348 275 223
150 54 50 45 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2471 2516 2520 3415 1537
Size: 275 659 45 4 895 4090
Fragments arranged by size:
4090 895 659 275 45 4
EarI CTCTTCn'nnn_
Cuts at: 1353 3898 1353
Size: 2545 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4555 4929 5075 387
Size: 1064 138 2966 374 146 1280
Fragments arranged by size:
2966 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2507 3251 4031 4628 5415 5683 1239
Size: 393 875 744 780 597 787 268 1524
Fragments arranged by size:
1524 875 787 780 744 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2028 2029 2281 2290 2977 3062 3165 2028
Size: 1 252 9 687 85 103 4831
Fragments arranged by size:
4831 687 252 103 85 9 1
EcoRI G'AATT_C
Cuts at: 2137 3277 3655 2137
Size: 1140 378 4450
Fragments arranged by size:
4450 1140 378
FseI GG_CCGG'CC
Cuts at: 2522 2522
Size: 5968
FspI TGC'GCA
Cuts at: 4474 4474
Size: 5968
HaeII r_GCGC'y
Cuts at: 1678 5115 1678
Size: 3437 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2097 2129 2544 2576 2855
Size: 32 135 32 525 32 415 32 279
Cuts at: 2855 2887 4609 4641 4683 4715 1373
Size: 32 1722 32 42 32 2626
Fragments arranged by size:
2626 1722 525 415 279 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2129 3312 3558 669
Size: 988 281 191 1183 246 3079
Fragments arranged by size:
3079 1183 988 281 246 191
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5968
HpaI GTT'AAC
Cuts at: 3558 3558
Size: 5968
KpnI G_GTAC'C
Cuts at: 3293 3293
Size: 5968
MfeI C'AATT_G
Cuts at: 2274 3567 2274
Size: 1293 4675
MluI A'CGCG_T
Cuts at: 5844 5844
Size: 5968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5082 5266 1305
Size: 3777 184 2007
Fragments arranged by size:
3777 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2055 2672 10
Size: 54 1425 343 223 617 3306
Fragments arranged by size:
3306 1425 617 343 223 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3926 4285 4444 518
Size: 648 2760 359 159 2042
Fragments arranged by size:
2760 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2941 3384 4061 5209 5454 1606
Size: 1335 443 677 1148 245 2120
Fragments arranged by size:
2120 1335 1148 677 443 245
NaeI GCC'GGC
Cuts at: 2520 2520
Size: 5968
NcoI C'CATG_G
Cuts at: 513 1065 2836 513
Size: 552 1771 3645
Fragments arranged by size:
3645 1771 552
NdeI CA'TA_TG
Cuts at: 387 2956 387
Size: 2569 3399
NgoMIV G'CCGG_C
Cuts at: 2518 2518
Size: 5968
NheI G'CTAG_C
Cuts at: 1052 3089 1052
Size: 2037 3931
NotI GC'GGCC_GC
Cuts at: 3415 3415
Size: 5968
NspI r_CATG'y
Cuts at: 1132 1229 3251 3326 4871 1132
Size: 97 2022 75 1545 2229
Fragments arranged by size:
2229 2022 1545 97 75
PasI CC'CwG_GG
Cuts at: 1348 2251 1348
Size: 903 5065
PciI A'CATG_T
Cuts at: 4867 4867
Size: 5968
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2380 2401 1108
Size: 902 370 21 4675
Fragments arranged by size:
4675 902 370 21
PfoI T'CCnGG_A
Cuts at: 2740 2740
Size: 5968
PmeI GTTT'AAAC
Cuts at: 3272 3272
Size: 5968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 5550 5582 4037
Size: 32 1481 32 4423
Fragments arranged by size:
4423 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2281 3062 3165 2281
Size: 781 103 5084
Fragments arranged by size:
5084 781 103
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5968
PsiI TTA'TAA
Cuts at: 3538 3538
Size: 5968
PspOMI G'GGCC_C
Cuts at: 2028 2028
Size: 5968
PstI C_TGCA'G
Cuts at: 830 2226 2289 3211 3320 830
Size: 1396 63 922 109 3478
Fragments arranged by size:
3478 1396 922 109 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5968
PvuII CAG'CTG
Cuts at: 2941 2941
Size: 5968
SalI G'TCGA_C
Cuts at: 1936 3310 1936
Size: 1374 4594
SanDI GG'GwC_CC
Cuts at: 3062 3062
Size: 5968
SbfI CC_TGCA'GG
Cuts at: 2289 3320 2289
Size: 1031 4937
ScaI AGT'ACT
Cuts at: 4216 5928 4216
Size: 1712 4256
SfcI C'TryA_G
Cuts at: 826 1045 2222 2285 3207 3316 4451 5132 5323
Size: 219 1177 63 922 109 1135 681 191
Cuts at: 5323 826
Size: 1471
Fragments arranged by size:
1471 1177 1135 922 681 219 191 109
63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5968
SmaI CCC'GGG
Cuts at: 1872 3295 1872
Size: 1423 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4072 4973 5235 820
Size: 197 926 2129 901 262 1553
Fragments arranged by size:
2129 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3759 493
Size: 3266 2702
SpeI A'CTAG_T
Cuts at: 152 2131 152
Size: 1979 3989
SphI G_CATG'C
Cuts at: 1132 3326 1132
Size: 2194 3774
SspI AAT'ATT
Cuts at: 5 52 3892 5
Size: 47 3840 2081
Fragments arranged by size:
3840 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5968
StyI C'CwwG_G
Cuts at: 513 1065 2836 3407 513
Size: 552 1771 571 3074
Fragments arranged by size:
3074 1771 571 552
SwaI ATTT'AAAT
Cuts at: 2233 2233
Size: 5968
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3995 4012 4165 4350 4795 325
Size: 1255 2415 17 153 185 445 1498
Fragments arranged by size:
2415 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2482 4214 5926
Size: 274 80 33 51 1443 505 1732 1712
Cuts at: 5926 96
Size: 138
Fragments arranged by size:
1732 1712 1443 505 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3067 3099 5928 5960 3067
Size: 32 2829 32 3075
Fragments arranged by size:
3075 2829 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2728 1316
Size: 1412 4556
XbaI T'CTAG_A
Cuts at: 3304 3304
Size: 5968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2775 2857 2775
Size: 82 5886
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5968
XmaI C'CCGG_G
Cuts at: 1870 3293 1870
Size: 1423 4545
XmnI GAAnn'nnTTC
Cuts at: 4097 4097
Size: 5968
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3776 276
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI