################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI MwoI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TspRI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 478 GGTACC G^GTACC AlwNI 248 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 271 ATTAAT AT^TAAT AsnI 271 ATTAAT AT^TAAT Asp718I 478 GGTACC G^GTACC AspHI 501 GAGCTC G[AT]GC[AT]^C AspI 40 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 478 GGTACC G^G[CT][GA]CC BanII 501 GAGCTC G[GA]GC[CT]^C BfaI 262 CTAG C^TAG BmyI 501 GAGCTC G[AGT]GC[ACT]^C BseRI 451 GAGGAG GAGGAG(10/8) BsiHKAI 501 GAGCTC G[AT]GC[AT]^C BsmFI 485 GGGAC GGGAC(10/14) Bsp1286I 501 GAGCTC G[AGT]GC[ACT]^C BspHI 209 TCATGA T^CATGA BsrI 11 CCAGT ACTGG(1/-1) BstYI 357 AGATCC [GA]^GATC[CT] CfoI 457 GCGC GCG^C DdeI 329 CTCAG C^T[ACGT]AG DsaI 446 CCACGG C^C[GA][CT]GG EarI 472 GAAGAG CTCTTC(1/4) HaeII 456 GGCGCT [GA]GCGC^[CT] HhaI 457 GCGC GCG^C HinP1I 457 GCGC G^CGC HphI 370 GGTGA GGTGA(8/7) KpnI 478 GGTACC GGTAC^C Ksp632I 472 GAAGAG CTCTTC(1/4) MaeI 262 CTAG C^TAG MaeII 41 ACGT A^CGT MflI 357 AGATCC [GA]^GATC[CT] MslI 36 CACGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 134 CATATG CA^TATG NlaIV 478 GGTACC GG[ACGT]^[ACGT]CC PflFI 40 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 534 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 316 GAGTC GAGTC(4/5) PshBI 271 ATTAAT AT^TAAT PstI 377 CTGCAG CTGCA^G RcaI 209 TCATGA T^CATGA SacI 501 GAGCTC GAGCT^C TaqI 56 TCGA T^CGA TfiI 206 GATTC G^A[AT]TC Tsp45I 371 GTGAC ^GT[GC]AC Tth111I 40 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 56 TCGA T^CGA Van91I 534 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 261 TCTAGA T^CTAGA XhoII 357 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 27 GCGG CCGC(-3/-1) 143 GCGG AcsI 97 GAATTT [GA]^AATT[CT] 218 AAATTT AlwI 78 GATCC GGATC(4/5) 358 GATCC ApoI 97 GAATTT [GA]^AATT[CT] 218 AAATTT AvaII 118 GGACC G^G[AT]CC 165 GGACC BbvI 379 GCAGC GCAGC(8/12) 376 GCTGC BcnI 16 CCGGG CC^[GC]GG 482 CCGGG BstNI 81 CCTGG CC^[AT]GG 534 CCAGG EcoRII 81 CCTGG ^CC[AT]GG 534 CCAGG Fnu4HI 376 GCTGC GC^[ACGT]GC 379 GCAGC HaeIII 339 GGCC GG^CC 531 GGCC HapII 16 CCGG C^CGG 482 CCGG HgaI 373 GACGC GACGC(5/10) 244 GCGTC HinfI 206 GATTC G^A[ACGT]TC 316 GAGTC HpaII 16 CCGG C^CGG 482 CCGG ItaI 376 GCTGC GC^[ACGT]GC 379 GCAGC MaeIII 73 GTTAC ^GT[ACGT]AC 371 GTGAC MspI 16 CCGG C^CGG 482 CCGG MvaI 81 CCTGG CC^[AT]GG 534 CCAGG NciI 16 CCGGG CC^[GC]GG 482 CCGGG SfcI 200 CTATAG C^T[GA][CT]AG 377 CTGCAG TseI 376 GCTGC G^C[AT]GC 379 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AfaI AluI BsaJI BsiYI BslI BssKI BstF5I Cfr13I DpnII FokI MboI MboII MnlI MseI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI Tru9I Tsp509I