################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI TseI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AfaI 204 GTAC GT^AC AlwI 270 GATCC GGATC(4/5) AspHI 283 GAGCAC G[AT]GC[AT]^C AviII 317 TGCGCA TGC^GCA BciVI 123 GGATAC GTATCC(6/5) BcnI 171 CCGGG CC^[GC]GG BmyI 283 GAGCAC G[AGT]GC[ACT]^C BpmI 265 CTGGAG CTGGAG(16/14) BsiHKAI 283 GAGCAC G[AT]GC[AT]^C BsmAI 35 GAGAC GTCTC(1/5) BsmBI 35 GAGACG CGTCTC(1/5) Bsp1286I 283 GAGCAC G[AGT]GC[ACT]^C BspMI 350 ACCTGC ACCTGC(4/8) BstXI 101 CCAATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 269 AGATCC [GA]^GATC[CT] Cac8I 225 GCATGC GC[ACGT]^[ACGT]GC Cfr13I 323 GGGCC G^G[ACGT]CC FspI 317 TGCGCA TGC^GCA HaeIII 324 GGCC GG^CC HinfI 307 GACTC G^A[ACGT]TC HphI 48 GGTGA GGTGA(8/7) MaeIII 49 GTGAC ^GT[ACGT]AC MflI 269 AGATCC [GA]^GATC[CT] MseI 83 TTAA T^TAA MspA1I 257 CCGCTG C[AC]G^C[GT]G MwoI 229 GCCCTGGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 171 CCGGG CC^[GC]GG NdeI 290 CATATG CA^TATG PleI 307 GACTC GAGTC(4/5) RsaI 204 GTAC GT^AC Sau96I 323 GGGCC G^G[ACGT]CC SfaNI 185 GCATC GCATC(5/9) SphI 225 GCATGC GCATG^C TaqI 143 TCGA T^CGA Tru9I 83 TTAA T^TAA Tsp45I 49 GTGAC ^GT[GC]AC TthHB8I 143 TCGA T^CGA XcmI 101 CCAATTTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 269 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 235 GACGCC G[GA]^CG[CT]C 295 GACGCC AluI 17 AGCT AG^CT 80 AGCT BbsI 145 GAAGAC GAAGAC(2/6) 346 GAAGAC BpuAI 145 GAAGAC GAAGAC(2/6) 346 GAAGAC BsaHI 235 GACGCC G[GA]^CG[CT]C 295 GACGCC BsrI 52 ACTGG ACTGG(1/-1) 201 CCAGT CfoI 318 GCGC GCG^C 354 GCGC EcoT14I 278 CCAAGG C^C[AT][AT]GG 302 CCAAGG FauI 256 CCCGC CCCGC(4/6) 4 GCGGG HapII 156 CCGG C^CGG 171 CCGG HgaI 235 GACGC GACGC(5/10) 295 GACGC HhaI 318 GCGC GCG^C 354 GCGC Hin1I 235 GACGCC G[GA]^CG[CT]C 295 GACGCC HinP1I 318 GCGC G^CGC 354 GCGC HpaII 156 CCGG C^CGG 171 CCGG MboII 145 GAAGA GAAGA(8/7) 346 GAAGA MslI 21 CATCGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 286 CACCCATATG MspI 156 CCGG C^CGG 171 CCGG NlaIII 226 CATG CATG^ 333 CATG NspI 225 GCATGC [GA]CATG^[CT] 332 GCATGT StyI 278 CCAAGG C^C[AT][AT]GG 302 CCAAGG Tsp509I 103 AATT ^AATT 161 AATT TspRI 26 CAGTG CA[GC]TG[ACGT][ACGT]^ 339 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AciI BsaJI BsiYI BslI BssKI BstF5I BstNI DpnII EcoRII Fnu4HI FokI ItaI MboI MnlI MvaI NdeII Sau3AI ScrFI