################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspEI AspI BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I HgaI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 460 CGCG CG^CG AcsI 797 AAATTC [GA]^AATT[CT] ApaI 179 GGGCCC GGGCC^C ApoI 797 AAATTC [GA]^AATT[CT] AseI 345 ATTAAT AT^TAAT AsnI 345 ATTAAT AT^TAAT Asp700I 166 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1328 CCCGAG C^[CT]CG[GA]G AviII 1550 TGCGCA TGC^GCA AvrII 436 CCTAGG C^CTAGG BalI 1388 TGGCCA TGG^CCA BamHI 1772 GGATCC G^GATCC BbsI 390 GAAGAC GAAGAC(2/6) BclI 843 TGATCA T^GATCA BglI 319 GCCAAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 436 CCTAGG C^CTAGG BpuAI 390 GAAGAC GAAGAC(2/6) BsgI 1471 CTGCAC GTGCAG(16/14) BsmI 1119 GCATTC GAATGC(1/-1) BsoBI 1328 CCCGAG C^[CT]CG[GA]G BspHI 1148 TCATGA T^CATGA BssSI 1648 CACGAG CACGAG(-5/-1) BstAPI 2242 GCACCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 460 CGCG CG^CG BstXI 1065 CCAACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 566 CCGTGG C^C[GA][CT]GG EcoNI 2230 CCTTCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 843 TGATCA T^GATCA FseI 3 GGCCGGCC GGCCGG^CC FspI 1550 TGCGCA TGC^GCA HaeII 2013 GGCGCT [GA]GCGC^[CT] HincII 1240 GTTGAC GT[CT]^[GA]AC HindII 1240 GTTGAC GT[CT]^[GA]AC HindIII 1273 AAGCTT A^AGCTT MaeII 221 ACGT A^CGT MluNI 1388 TGGCCA TGG^CCA MscI 1388 TGGCCA TGG^CCA MvnI 460 CGCG CG^CG PshBI 345 ATTAAT AT^TAAT PstI 1361 CTGCAG CTGCA^G RcaI 1148 TCATGA T^CATGA SpeI 1531 ACTAGT A^CTAGT SphI 1653 GCATGC GCATG^C XcmI 1955 CCAAGGCTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 166 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AspHI 1645 GAGCAC G[AT]GC[AT]^C 2240 GAGCAC BanI 1306 GGCACC G^G[CT][GA]CC 1899 GGTGCC BanII 179 GGGCCC G[GA]GC[CT]^C 1926 GGGCTC BcnI 1310 CCCGG CC^[GC]GG 1943 CCGGG BpmI 1190 CTCCAG CTGGAG(16/14) 58 CTGGAG BsiHKAI 1645 GAGCAC G[AT]GC[AT]^C 2240 GAGCAC BstEII 780 GGTGACC G^GT[ACGT]ACC 1251 GGTGACC BstPI 780 GGTGACC G^GT[ACGT]ACC 1251 GGTGACC BstYI 1772 GGATCC [GA]^GATC[CT] 1798 AGATCC EcoO65I 780 GGTGACC G^GT[ACGT]ACC 1251 GGTGACC EcoT14I 436 CCTAGG C^C[AT][AT]GG 1955 CCAAGG MflI 1772 GGATCC [GA]^GATC[CT] 1798 AGATCC MslI 519 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1648 CACGAGCATG MspA1I 55 CAGCTG C[AC]G^C[GT]G 2194 CAGCTG NaeI 4 GCCGGC GCC^GGC 1226 GCCGGC NciI 1310 CCCGG CC^[GC]GG 1943 CCGGG NgoMIV 4 GCCGGC G^CCGGC 1226 GCCGGC NspI 127 GCATGT [GA]CATG^[CT] 1653 GCATGC PleI 1033 GACTC GAGTC(4/5) 1152 GACTC PpuMI 2006 AGGTCCT [GA]G^G[AT]CC[CT] 2226 GGGACCT PvuII 55 CAGCTG CAG^CTG 2194 CAGCTG StyI 436 CCTAGG C^C[AT][AT]GG 1955 CCAAGG XhoII 1772 GGATCC [GA]^GATC[CT] 1798 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BbvI BfaI BmyI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I