(Circular) (Six-base) MAPSORT of: pFN21AE5757.seq Check: 5545 from: 1 to: 6982 pFN21AE5757 6982 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3967 3967 Size: 6982 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4792 278 Size: 53 83 186 936 3256 2468 Fragments arranged by size: 3256 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4325 1937 Size: 2388 4594 Acc65I G'GTAC_C Cuts at: 4303 4303 Size: 6982 AclI AA'CG_TT Cuts at: 1563 5109 5482 1563 Size: 3546 373 3063 Fragments arranged by size: 3546 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3266 3440 3734 3987 5045 6429 6697 1632 Size: 1634 174 294 253 1058 1384 268 1917 Fragments arranged by size: 1917 1634 1384 1058 294 268 253 174 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6785 AflIII A'CryG_T Cuts at: 1203 5881 6858 1203 Size: 4678 977 1327 Fragments arranged by size: 4678 1327 977 AgeI A'CCGG_T Cuts at: 5813 5813 Size: 6982 AhdI GACnn_n'nnGTC Cuts at: 5711 5711 Size: 6982 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6982 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3122 3675 1184 Size: 1938 553 4491 Fragments arranged by size: 4491 1938 553 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2057 2089 2057 Size: 32 6950 AlwNI CAG_nnn'CTG Cuts at: 2558 2834 3471 3622 3848 4725 6297 2558 Size: 276 637 151 226 877 1572 3243 Fragments arranged by size: 3243 1572 877 637 276 226 151 ApaI G_GGCC'C Cuts at: 2188 2188 Size: 6982 ApaLI G'TGCA_C Cuts at: 5039 6195 5039 Size: 1156 5826 ApoI r'AATT_y Cuts at: 1493 2802 4291 4517 4669 6778 1493 Size: 1309 1489 226 152 2109 1697 Fragments arranged by size: 2109 1697 1489 1309 226 152 AseI AT'TA_AT Cuts at: 160 1032 2351 5536 160 Size: 872 1319 3185 1606 Fragments arranged by size: 3185 1606 1319 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6982 AvaI C'yCGr_G Cuts at: 1870 1943 3333 4307 1870 Size: 73 1390 974 4545 Fragments arranged by size: 4545 1390 974 73 AvrII C'CTAG_G Cuts at: 2441 2441 Size: 6982 BamHI G'GATC_C Cuts at: 3777 4312 4679 3777 Size: 535 367 6080 Fragments arranged by size: 6080 535 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3311 3904 4303 5758 618 Size: 325 217 627 1524 593 399 1455 1842 Fragments arranged by size: 1842 1524 1455 627 593 399 325 217 BanII G_rGCy'C Cuts at: 1391 2188 3935 4301 1391 Size: 797 1747 366 4072 Fragments arranged by size: 4072 1747 797 366 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2402 928 Size: 971 503 5508 Fragments arranged by size: 5508 971 503 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5173 5207 1903 Size: 34 3236 34 3678 Fragments arranged by size: 3678 3236 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4874 6084 4874 Size: 1210 5772 BclI T'GATC_A Cuts at: 1548 2848 1548 Size: 1300 5682 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2330 5593 136 Size: 107 122 71 631 605 658 3263 1525 Fragments arranged by size: 3263 1525 658 631 605 122 107 71 BglII A'GATC_T Cuts at: 6977 6977 Size: 6982 BlpI GC'TnA_GC Cuts at: 4399 4399 Size: 6982 Bme1580I G_kGCm'C Cuts at: 1790 2188 2297 3909 5043 6199 1790 Size: 398 109 1612 1134 1156 2573 Fragments arranged by size: 2573 1612 1156 1134 398 109 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6982 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3142 4713 5671 Size: 416 53 83 399 24 1725 1571 958 Cuts at: 5671 442 Size: 1753 Fragments arranged by size: 1753 1725 1571 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6982 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 3180 5642 1239 Size: 845 1096 2462 2579 Fragments arranged by size: 2579 2462 1096 845 Bpu10I CC'TnA_GC Cuts at: 2366 4167 2366 Size: 1801 5181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2174 3144 3224 3419 5107 5972 6270 2174 Size: 970 80 195 1688 865 298 2886 Fragments arranged by size: 2886 1688 970 865 298 195 80 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2818 3576 3712 5645 882 Size: 617 1319 758 136 1933 2219 Fragments arranged by size: 2219 1933 1319 758 617 136 BsaAI yAC'GTr Cuts at: 493 1204 4773 493 Size: 711 3569 2702 Fragments arranged by size: 3569 2702 711 BsaBI GATnn'nnATC Cuts at: 6976 6976 Size: 6982 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4789 5171 5806 275 Size: 53 83 186 936 3256 382 635 1451 Fragments arranged by size: 3256 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5415 5813 6087 6234 6645 5415 Size: 398 274 147 411 5752 Fragments arranged by size: 5752 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2057 2087 1178 Size: 30 165 30 654 30 6073 Fragments arranged by size: 6073 654 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2873 2948 4276 1186 Size: 1687 75 1328 3892 Fragments arranged by size: 3892 1687 1328 75 BseYI C'CCAG_C Cuts at: 3353 6185 6681 3353 Size: 2832 496 3654 Fragments arranged by size: 3654 2832 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3461 1163 Size: 573 1725 4684 Fragments arranged by size: 4684 1725 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4432 5193 6221 1540 Size: 275 186 2431 761 1028 2301 Fragments arranged by size: 2431 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3654 4249 5043 5128 6199 3654 Size: 595 794 85 1071 4437 Fragments arranged by size: 4437 1071 794 595 85 BsmI GAATG_Cn' Cuts at: 3124 4493 4586 3124 Size: 1369 93 5520 Fragments arranged by size: 5520 1369 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2188 2297 3654 3909 3935 4249 4301 Size: 399 398 109 1357 255 26 314 52 Cuts at: 4301 5043 5128 6199 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1357 1071 742 399 398 314 255 109 85 52 26 BspEI T'CCGG_A Cuts at: 6645 6645 Size: 6982 BspHI T'CATG_A Cuts at: 3153 4871 6601 3153 Size: 1718 1730 3534 Fragments arranged by size: 3534 1730 1718 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2495 3967 4138 4337 844 Size: 1651 1472 171 199 3489 Fragments arranged by size: 3489 1651 1472 199 171 BsrBI CCG'CTC Cuts at: 1394 1823 4869 1394 Size: 429 3046 3507 Fragments arranged by size: 3507 3046 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5477 5651 65 Size: 1183 4229 174 1396 Fragments arranged by size: 4229 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 2089 3231 5626 5813 1700 Size: 110 199 80 1142 2395 187 2869 Fragments arranged by size: 2869 2395 1142 199 187 110 80 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6982 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6982 BssSI C'ACGA_G Cuts at: 3653 5042 6054 3653 Size: 1389 1012 4581 Fragments arranged by size: 4581 1389 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4253 794 Size: 3459 3523 BstBI TT'CG_AA Cuts at: 5786 5786 Size: 6982 BstEII G'GTnAC_C Cuts at: 1184 2785 3256 1184 Size: 1601 471 4910 Fragments arranged by size: 4910 1601 471 BstXI CCAn_nnnn'nTGG Cuts at: 3077 3077 Size: 6982 BstYI r'GATC_y Cuts at: 1972 3777 3803 4312 4679 5064 5081 6533 6619 Size: 1805 26 509 367 385 17 1452 86 Cuts at: 6619 6631 6977 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1805 1452 509 385 367 346 86 26 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2571 513 Size: 552 939 567 4924 Fragments arranged by size: 4924 939 567 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6243 Fragments arranged by size: 6243 683 56 BtsI GCAGTG_nn' Cuts at: 2059 2105 2851 4498 5292 5312 2059 Size: 46 746 1647 794 20 3729 Fragments arranged by size: 3729 1647 794 746 46 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6982 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6947 DraI TTT'AAA Cuts at: 4286 4286 Size: 6982 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5989 809 Size: 507 4673 1802 Fragments arranged by size: 4673 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3229 3393 Size: 54 1425 50 275 18 177 1222 164 Cuts at: 3393 4429 5318 6947 8 Size: 1036 889 1629 43 Fragments arranged by size: 1629 1425 1222 1036 889 275 177 164 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4429 1537 Size: 275 2617 4090 Fragments arranged by size: 4090 2617 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2673 2685 2754 2760 2804 2969 3710 3914 Size: 1320 12 69 6 44 165 741 204 Cuts at: 3914 3995 4912 1353 Size: 81 917 3423 Fragments arranged by size: 3423 1320 917 741 204 165 81 69 44 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3665 5569 5943 6089 387 Size: 1064 138 2076 1904 374 146 1280 Fragments arranged by size: 2076 1904 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 3180 3266 3440 3734 3987 5045 Size: 393 452 1096 86 174 294 253 1058 Cuts at: 5045 5642 6429 6697 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1096 1058 787 597 452 393 294 268 253 174 86 EcoNI CCTnn'n_nnAGG Cuts at: 4239 4239 Size: 6982 EcoO109I rG'GnC_Cy Cuts at: 2184 2185 4012 4037 4232 2184 Size: 1 1827 25 195 4934 Fragments arranged by size: 4934 1827 195 25 1 EcoRI G'AATT_C Cuts at: 4291 4669 4291 Size: 378 6604 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2119 3071 3265 2119 Size: 952 194 5836 Fragments arranged by size: 5836 952 194 FseI GG_CCGG'CC Cuts at: 2013 2013 Size: 6982 FspI TGC'GCA Cuts at: 3557 5488 3557 Size: 1931 5051 FspAI rTGC'GCAy Cuts at: 3557 3557 Size: 6982 HaeII r_GCGC'y Cuts at: 1678 4022 6129 1678 Size: 2344 2107 2531 Fragments arranged by size: 2531 2344 2107 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2585 2617 4136 4168 5623 Size: 32 135 32 1013 32 1519 32 1455 Cuts at: 5623 5655 5697 5729 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1519 1455 1013 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3247 4326 4572 669 Size: 988 281 1309 1079 246 3079 Fragments arranged by size: 3079 1309 1079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3278 748 Size: 2530 4452 HpaI GTT'AAC Cuts at: 4572 4572 Size: 6982 KpnI G_GTAC'C Cuts at: 4307 4307 Size: 6982 MfeI C'AATT_G Cuts at: 4581 4581 Size: 6982 MluI A'CGCG_T Cuts at: 6858 6858 Size: 6982 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3094 6096 6280 1305 Size: 1789 3002 184 2007 Fragments arranged by size: 3002 2007 1789 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3395 10 Size: 54 1425 343 1563 3597 Fragments arranged by size: 3597 1563 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2528 3657 4940 5299 5458 518 Size: 648 1362 1129 1283 359 159 2042 Fragments arranged by size: 2042 1362 1283 1129 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2062 4201 4398 5075 6223 6468 1606 Size: 456 2139 197 677 1148 245 2120 Fragments arranged by size: 2139 2120 1148 677 456 245 197 NaeI GCC'GGC Cuts at: 2011 3233 2011 Size: 1222 5760 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5491 Fragments arranged by size: 5491 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6982 NgoMIV G'CCGG_C Cuts at: 2009 3231 2009 Size: 1222 5760 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6982 NotI GC'GGCC_GC Cuts at: 4429 4429 Size: 6982 NspI r_CATG'y Cuts at: 1132 1229 2136 3662 4340 5885 1132 Size: 97 907 1526 678 1545 2229 Fragments arranged by size: 2229 1545 1526 907 678 97 PasI CC'CwG_GG Cuts at: 1348 3622 1348 Size: 2274 4708 PciI A'CATG_T Cuts at: 5881 5881 Size: 6982 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6982 PfoI T'CCnGG_A Cuts at: 2424 2424 Size: 6982 PmeI GTTT'AAAC Cuts at: 4286 4286 Size: 6982 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2057 2089 5051 5083 6564 6596 2057 Size: 32 2962 32 1481 32 2443 Fragments arranged by size: 2962 2443 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4012 4232 4012 Size: 220 6762 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6982 PsiI TTA'TAA Cuts at: 4552 4552 Size: 6982 PspOMI G'GGCC_C Cuts at: 2184 2184 Size: 6982 PstI C_TGCA'G Cuts at: 830 3370 4334 830 Size: 2540 964 3478 Fragments arranged by size: 3478 2540 964 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6982 PvuII CAG'CTG Cuts at: 2062 4201 2062 Size: 2139 4843 SalI G'TCGA_C Cuts at: 1936 4324 1936 Size: 2388 4594 SbfI CC_TGCA'GG Cuts at: 4334 4334 Size: 6982 ScaI AGT'ACT Cuts at: 5230 6942 5230 Size: 1712 5270 SfcI C'TryA_G Cuts at: 826 1045 2613 3366 3495 4330 5465 6146 6337 Size: 219 1568 753 129 835 1135 681 191 Cuts at: 6337 826 Size: 1471 Fragments arranged by size: 1568 1471 1135 835 753 681 219 191 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6982 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6982 SmaI CCC'GGG Cuts at: 1872 4309 1872 Size: 2437 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2189 3159 3239 3434 5086 5987 Size: 197 926 246 970 80 195 1652 901 Cuts at: 5987 6249 820 Size: 262 1553 Fragments arranged by size: 1652 1553 970 926 901 262 246 197 195 80 SnaBI TAC'GTA Cuts at: 493 4773 493 Size: 4280 2702 SpeI A'CTAG_T Cuts at: 152 3536 152 Size: 3384 3598 SphI G_CATG'C Cuts at: 1132 3662 4340 1132 Size: 2530 678 3774 Fragments arranged by size: 3774 2530 678 SspI AAT'ATT Cuts at: 5 52 4906 5 Size: 47 4854 2081 Fragments arranged by size: 4854 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6982 StyI C'CwwG_G Cuts at: 513 1065 2004 2441 3960 4421 513 Size: 552 939 437 1519 461 3074 Fragments arranged by size: 3074 1519 939 552 461 437 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5009 5026 5179 5364 5809 325 Size: 1255 3429 17 153 185 445 1498 Fragments arranged by size: 3429 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5228 6940 96 Size: 274 80 33 51 1443 3251 1712 138 Fragments arranged by size: 3251 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6942 6974 6942 Size: 32 6950 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6982 XbaI T'CTAG_A Cuts at: 4318 4318 Size: 6982 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3967 3967 Size: 6982 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6982 XmaI C'CCGG_G Cuts at: 1870 4307 1870 Size: 2437 4545 XmnI GAAnn'nnTTC Cuts at: 2175 5111 2175 Size: 2936 4046 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4790 276 Size: 53 83 186 936 3256 2468 Fragments arranged by size: 3256 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI