(Linear) (Six-base) MAPSORT of: KIEE5757.seq Check: 650 from: 1 to: 2277 KIEE5757.orf With 182 enzymes: SgfI * December 5, 2014 13:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1962 2277 Size: 1962 315 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1261 1435 1729 1982 2277 Size: 1261 174 294 253 295 Fragments arranged by size: 1261 295 294 253 174 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1117 1670 2277 Size: 1117 553 607 Fragments arranged by size: 1117 607 553 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 52 84 2277 Size: 52 32 2193 Fragments arranged by size: 2193 52 32 AlwNI CAG_nnn'CTG Cuts at: 0 553 829 1466 1617 1843 2277 Size: 553 276 637 151 226 434 Fragments arranged by size: 637 553 434 276 226 151 ApaI G_GGCC'C Cuts at: 0 183 2277 Size: 183 2094 ApoI r'AATT_y Cuts at: 0 797 2277 Size: 797 1480 AseI AT'TA_AT Cuts at: 0 346 2277 Size: 346 1931 AvaI C'yCGr_G Cuts at: 0 1328 2277 Size: 1328 949 AvrII C'CTAG_G Cuts at: 0 436 2277 Size: 436 1841 BamHI G'GATC_C Cuts at: 0 1772 2277 Size: 1772 505 BanI G'GyrC_C Cuts at: 0 1306 1899 2277 Size: 1306 593 378 Fragments arranged by size: 1306 593 378 BanII G_rGCy'C Cuts at: 0 183 1930 2277 Size: 183 1747 347 Fragments arranged by size: 1747 347 183 BbsI GAAGACnn'nnnn_ Cuts at: 0 397 2277 Size: 397 1880 BclI T'GATC_A Cuts at: 0 843 2277 Size: 843 1434 BglI GCCn_nnn'nGGC Cuts at: 0 325 2277 Size: 325 1952 Bme1580I G_kGCm'C Cuts at: 0 183 292 1904 2277 Size: 183 109 1612 373 Fragments arranged by size: 1612 373 183 109 BmrI ACTGGGnnnn_n' Cuts at: 0 1137 2277 Size: 1137 1140 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 1175 2277 Size: 79 1096 1102 Fragments arranged by size: 1102 1096 79 Bpu10I CC'TnA_GC Cuts at: 0 361 2162 2277 Size: 361 1801 115 Fragments arranged by size: 1801 361 115 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 1139 1219 1414 2277 Size: 169 970 80 195 863 Fragments arranged by size: 970 863 195 169 80 BsaI GGTCTCn'nnnn_ Cuts at: 0 813 1571 1707 2277 Size: 813 758 136 570 Fragments arranged by size: 813 758 570 136 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 52 82 2277 Size: 52 30 2195 Fragments arranged by size: 2195 52 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 868 943 2271 2277 Size: 868 75 1328 6 Fragments arranged by size: 1328 868 75 6 BseYI C'CCAG_C Cuts at: 0 1348 2277 Size: 1348 929 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1456 2277 Size: 1456 821 BsiHKAI G_wGCw'C Cuts at: 0 1649 2244 2277 Size: 1649 595 33 Fragments arranged by size: 1649 595 33 BsmI GAATG_Cn' Cuts at: 0 1119 2277 Size: 1119 1158 Bsp1286I G_dGCh'C Cuts at: 0 183 292 1649 1904 1930 2244 2277 Size: 183 109 1357 255 26 314 33 Fragments arranged by size: 1357 314 255 183 109 33 26 BspHI T'CATG_A Cuts at: 0 1148 2277 Size: 1148 1129 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 490 1962 2133 2277 Size: 490 1472 171 144 Fragments arranged by size: 1472 490 171 144 BsrFI r'CCGG_y Cuts at: 0 4 84 1226 2277 Size: 4 80 1142 1051 Fragments arranged by size: 1142 1051 80 4 BssSI C'ACGA_G Cuts at: 0 1648 2277 Size: 1648 629 BstAPI GCAn_nnn'nTGC Cuts at: 0 2248 2277 Size: 2248 29 BstEII G'GTnAC_C Cuts at: 0 780 1251 2277 Size: 780 471 1026 Fragments arranged by size: 1026 780 471 BstXI CCAn_nnnn'nTGG Cuts at: 0 1072 2277 Size: 1072 1205 BstYI r'GATC_y Cuts at: 0 1772 1798 2277 Size: 1772 26 479 Fragments arranged by size: 1772 479 26 BtgI C'CryG_G Cuts at: 0 566 2277 Size: 566 1711 BtsI GCAGTG_nn' Cuts at: 0 54 100 846 2277 Size: 54 46 746 1431 Fragments arranged by size: 1431 746 54 46 EaeI y'GGCC_r Cuts at: 0 2 1224 1388 2277 Size: 2 1222 164 889 Fragments arranged by size: 1222 889 164 2 EarI CTCTTCn'nnn_ Cuts at: 0 668 680 749 755 799 964 1705 1909 Size: 668 12 69 6 44 165 741 204 Cuts at: 1909 1990 2277 Size: 81 287 Fragments arranged by size: 741 668 287 204 165 81 69 44 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1660 2277 Size: 1660 617 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 1175 1261 1435 1729 1982 2277 Size: 79 1096 86 174 294 253 295 Fragments arranged by size: 1096 295 294 253 174 86 79 EcoNI CCTnn'n_nnAGG Cuts at: 0 2234 2277 Size: 2234 43 EcoO109I rG'GnC_Cy Cuts at: 0 179 180 2007 2032 2227 2277 Size: 179 1 1827 25 195 50 Fragments arranged by size: 1827 195 179 50 25 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 114 1066 1260 2277 Size: 114 952 194 1017 Fragments arranged by size: 1017 952 194 114 FseI GG_CCGG'CC Cuts at: 0 8 2277 Size: 8 2269 FspI TGC'GCA Cuts at: 0 1552 2277 Size: 1552 725 FspAI rTGC'GCAy Cuts at: 0 1552 2277 Size: 1552 725 HaeII r_GCGC'y Cuts at: 0 2017 2277 Size: 2017 260 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 580 612 2131 2163 2277 Size: 580 32 1519 32 114 Fragments arranged by size: 1519 580 114 32 32 HincII GTy'rAC Cuts at: 0 1242 2277 Size: 1242 1035 HindIII A'AGCT_T Cuts at: 0 1273 2277 Size: 1273 1004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1089 2277 Size: 1089 1188 MscI TGG'CCA Cuts at: 0 1390 2277 Size: 1390 887 MslI CAynn'nnrTG Cuts at: 0 523 1652 2277 Size: 523 1129 625 Fragments arranged by size: 1129 625 523 MspA1I CmG'CkG Cuts at: 0 57 2196 2277 Size: 57 2139 81 Fragments arranged by size: 2139 81 57 NaeI GCC'GGC Cuts at: 0 6 1228 2277 Size: 6 1222 1049 Fragments arranged by size: 1222 1049 6 NgoMIV G'CCGG_C Cuts at: 0 4 1226 2277 Size: 4 1222 1051 Fragments arranged by size: 1222 1051 4 NspI r_CATG'y Cuts at: 0 131 1657 2277 Size: 131 1526 620 Fragments arranged by size: 1526 620 131 PasI CC'CwG_GG Cuts at: 0 1617 2277 Size: 1617 660 PfoI T'CCnGG_A Cuts at: 0 419 2277 Size: 419 1858 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 52 84 2277 Size: 52 32 2193 Fragments arranged by size: 2193 52 32 PpuMI rG'GwC_Cy Cuts at: 0 2007 2227 2277 Size: 2007 220 50 Fragments arranged by size: 2007 220 50 PspOMI G'GGCC_C Cuts at: 0 179 2277 Size: 179 2098 PstI C_TGCA'G Cuts at: 0 1365 2277 Size: 1365 912 PvuII CAG'CTG Cuts at: 0 57 2196 2277 Size: 57 2139 81 Fragments arranged by size: 2139 81 57 SfcI C'TryA_G Cuts at: 0 608 1361 1490 2277 Size: 608 753 129 787 Fragments arranged by size: 787 753 608 129 SmlI C'TyrA_G Cuts at: 0 184 1154 1234 1429 2277 Size: 184 970 80 195 848 Fragments arranged by size: 970 848 195 184 80 SpeI A'CTAG_T Cuts at: 0 1531 2277 Size: 1531 746 SphI G_CATG'C Cuts at: 0 1657 2277 Size: 1657 620 StyI C'CwwG_G Cuts at: 0 436 1955 2277 Size: 436 1519 322 Fragments arranged by size: 1519 436 322 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1962 2277 Size: 1962 315 XmnI GAAnn'nnTTC Cuts at: 0 170 2277 Size: 170 2107 Enzymes that do cut: AarI AcuI AlfI AloI AlwNI ApaI ApoI AseI AvaI AvrII BamHI BanI BanII BbsI BclI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI FseI FspI FspAI HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I NaeI NgoMIV NspI PasI PfoI PpiI PpuMI PspOMI PstI PvuII SfcI SmlI SpeI SphI StyI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI ApaLI AscI AsiSI BaeI BbeI BbvCI BcgI BciVI BfrBI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI ZraI