################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflIII AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstUI BstXI BstZ17I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 1810 GACGTC GACGT^C AciI 222 CCGC CCGC(-3/-1) AcyI 1810 GACGTC G[GA]^CG[CT]C AflII 169 CTTAAG C^TTAAG AgeI 543 ACCGGT A^CCGGT AlwI 82 GATCC GGATC(4/5) AlwNI 1238 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 631 CCTCAGG CC^T[ACGT]AGG BalI 1401 TGGCCA TGG^CCA BanII 574 GAGCCC G[GA]GC[CT]^C BbsI 1041 GTCTTC GAAGAC(2/6) BfrI 169 CTTAAG C^TTAAG BglII 1975 AGATCT A^GATCT BmyI 574 GAGCCC G[AGT]GC[ACT]^C BpuAI 1041 GTCTTC GAAGAC(2/6) BsaHI 1810 GACGTC G[GA]^CG[CT]C BsaJI 976 CCTGGG C^C[ACGT][ACGT]GG BsaWI 543 ACCGGT [AT]^CCGG[AT] BsiYI 1728 CCCTTCGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1728 CCCTTCGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 11 GGGAC GGGAC(10/14) Bsp1286I 574 GAGCCC G[AGT]GC[ACT]^C BspMI 1396 GCAGGT ACCTGC(4/8) BstBI 746 TTCGAA TT^CGAA BstEII 679 GGTCACC G^GT[ACGT]ACC BstPI 679 GGTCACC G^GT[ACGT]ACC Bsu36I 631 CCTCAGG CC^T[ACGT]AGG EaeI 1401 TGGCCA [CT]^GGCC[GA] Eco81I 631 CCTCAGG CC^T[ACGT]AGG EcoO65I 679 GGTCACC G^GT[ACGT]ACC EcoRI 712 GAATTC G^AATTC HgaI 827 GACGC GACGC(5/10) Hin1I 1810 GACGTC G[GA]^CG[CT]C MluNI 1401 TGGCCA TGG^CCA MscI 1401 TGGCCA TGG^CCA MspA1I 1433 CAGCTG C[AC]G^C[GT]G NdeI 1129 CATATG CA^TATG NlaIV 72 GGAACC GG[ACGT]^[ACGT]CC NspI 1072 GCATGC [GA]CATG^[CT] NspV 746 TTCGAA TT^CGAA PinAI 543 ACCGGT A^CCGGT PstI 1394 CTGCAG CTGCA^G PvuII 1433 CAGCTG CAG^CTG ScaI 481 AGTACT AGT^ACT SfuI 746 TTCGAA TT^CGAA SmlI 169 CTTAAG C^T[CT][GA]AG SpeI 1381 ACTAGT A^CTAGT SphI 1072 GCATGC GCATG^C TspRI 522 CACTG CA[GC]TG[ACGT][ACGT]^ XbaI 721 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BbvI 1333 GCAGC GCAGC(8/12) 1393 GCTGC BpmI 286 CTCCAG CTGGAG(16/14) 77 CTGGAG BsmAI 227 GTCTC GTCTC(1/5) 284 GTCTC BsrFI 543 ACCGGT [GA]^CCGG[CT] 1329 ACCGGC BstYI 81 AGATCC [GA]^GATC[CT] 1975 AGATCT Cfr10I 543 ACCGGT [GA]^CCGG[CT] 1329 ACCGGC Cfr13I 1186 GGCCC G^G[ACGT]CC 1726 GGCCC DraII 1185 AGGCCCT [GA]G^G[ACGT]CC[CT] 1725 AGGCCCT EarI 229 CTCTTC CTCTTC(1/4) 1847 CTCTTC EcoO109I 1185 AGGCCCT [GA]G^G[ACGT]CC[CT] 1725 AGGCCCT HapII 544 CCGG C^CGG 1330 CCGG HpaII 544 CCGG C^CGG 1330 CCGG Ksp632I 229 CTCTTC CTCTTC(1/4) 1847 CTCTTC MflI 81 AGATCC [GA]^GATC[CT] 1975 AGATCT MspI 544 CCGG C^CGG 1330 CCGG MwoI 889 GCTACAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1082 GCCTCCTTTGC NlaIII 1073 CATG CATG^ 1765 CATG Sau96I 1186 GGCCC G^G[ACGT]CC 1726 GGCCC SfaNI 47 GATGC GCATC(5/9) 1856 GATGC TseI 1333 GCAGC G^C[AT]GC 1393 GCTGC XhoII 81 AGATCC [GA]^GATC[CT] 1975 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AcsI AfaI AluI ApoI BfaI BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI NdeII PleI RsaI Sau3AI ScrFI SfcI TaqI TfiI Tru9I Tsp45I Tsp509I TthHB8I