(Circular) (Six-base) MAPSORT of: pFN21AE1175.seq Check: 7512 from: 1 to: 6697 pFN21AE1175 6697 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3392 3392 Size: 6697 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3819 4507 278 Size: 53 83 186 936 2283 688 2468 Fragments arranged by size: 2468 2283 936 688 186 83 53 AccI GT'mk_AC Cuts at: 1937 4040 1937 Size: 2103 4594 Acc65I G'GTAC_C Cuts at: 4018 4018 Size: 6697 AclI AA'CG_TT Cuts at: 1563 4824 5197 1563 Size: 3261 373 3063 Fragments arranged by size: 3261 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2221 3969 4760 6144 6412 1632 Size: 589 1748 791 1384 268 1917 Fragments arranged by size: 1917 1748 1384 791 589 268 AflII C'TTAA_G Cuts at: 820 1017 2174 820 Size: 197 1157 5343 Fragments arranged by size: 5343 1157 197 AflIII A'CryG_T Cuts at: 1203 5596 6573 1203 Size: 4393 977 1327 Fragments arranged by size: 4393 1327 977 AgeI A'CCGG_T Cuts at: 2548 5528 2548 Size: 2980 3717 AhdI GACnn_n'nnGTC Cuts at: 5426 5426 Size: 6697 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6697 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3100 1184 Size: 1916 4781 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3094 3126 3094 Size: 32 6665 AlwNI CAG_nnn'CTG Cuts at: 3248 4440 6012 3248 Size: 1192 1572 3933 Fragments arranged by size: 3933 1572 1192 ApaLI G'TGCA_C Cuts at: 4754 5910 4754 Size: 1156 5541 ApoI r'AATT_y Cuts at: 1493 2251 2270 2717 3554 4006 4232 4384 6493 Size: 758 19 447 837 452 226 152 2109 Cuts at: 6493 1493 Size: 1697 Fragments arranged by size: 2109 1697 837 758 452 447 226 152 19 AseI AT'TA_AT Cuts at: 160 1032 5251 160 Size: 872 4219 1606 Fragments arranged by size: 4219 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6697 AvaI C'yCGr_G Cuts at: 1870 1943 4022 1870 Size: 73 2079 4545 Fragments arranged by size: 4545 2079 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2550 2583 2550 Size: 33 6664 BamHI G'GATC_C Cuts at: 4027 4394 4027 Size: 367 6330 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4018 5473 618 Size: 325 217 627 2231 1455 1842 Fragments arranged by size: 2231 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2583 4016 1391 Size: 1192 1433 4072 Fragments arranged by size: 4072 1433 1192 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3039 928 Size: 971 1140 4586 Fragments arranged by size: 4586 1140 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4888 4922 1903 Size: 34 50 34 2867 34 3678 Fragments arranged by size: 3678 2867 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4589 5799 4589 Size: 1210 5487 BclI T'GATC_A Cuts at: 1548 1548 Size: 6697 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5308 136 Size: 107 122 71 631 605 3636 1525 Fragments arranged by size: 3636 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3980 6692 3980 Size: 2712 3985 BlpI GC'TnA_GC Cuts at: 4114 4114 Size: 6697 Bme1580I G_kGCm'C Cuts at: 1790 4758 5914 1790 Size: 2968 1156 2573 Fragments arranged by size: 2968 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6697 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4428 5386 442 Size: 416 53 83 399 24 3011 958 1753 Fragments arranged by size: 3011 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6697 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2103 2276 5357 1239 Size: 864 173 3081 2579 Fragments arranged by size: 3081 2579 864 173 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4822 5687 5985 4822 Size: 865 298 5534 Fragments arranged by size: 5534 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5360 882 Size: 617 3861 2219 Fragments arranged by size: 3861 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4488 493 Size: 711 3284 2702 Fragments arranged by size: 3284 2702 711 BsaBI GATnn'nnATC Cuts at: 6691 6691 Size: 6697 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3816 4504 4886 5521 Size: 53 83 186 936 2283 688 382 635 Cuts at: 5521 275 Size: 1451 Fragments arranged by size: 2283 1451 936 688 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2548 5130 5528 5802 5949 6360 2548 Size: 2582 398 274 147 411 2885 Fragments arranged by size: 2885 2582 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2274 2304 3265 3295 1178 Size: 30 165 30 871 30 961 30 4580 Fragments arranged by size: 4580 961 871 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6697 BseYI C'CCAG_C Cuts at: 5900 6396 5900 Size: 496 6201 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6124 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4147 4908 5936 1540 Size: 275 186 2146 761 1028 2301 Fragments arranged by size: 2301 2146 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4758 4843 5914 4758 Size: 85 1071 5541 Fragments arranged by size: 5541 1071 85 BsmI GAATG_Cn' Cuts at: 4208 4301 4208 Size: 93 6604 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2583 4016 4758 4843 5914 1391 Size: 399 793 1433 742 85 1071 2174 Fragments arranged by size: 2174 1433 1071 793 742 399 85 BspEI T'CCGG_A Cuts at: 6360 6360 Size: 6697 BspHI T'CATG_A Cuts at: 4586 6316 4586 Size: 1730 4967 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3392 4052 844 Size: 2548 660 3489 Fragments arranged by size: 3489 2548 660 BsrBI CCG'CTC Cuts at: 1394 1823 4584 1394 Size: 429 2761 3507 Fragments arranged by size: 3507 2761 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5192 5366 65 Size: 1183 3944 174 1396 Fragments arranged by size: 3944 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2548 3334 5341 5528 1700 Size: 110 738 786 2007 187 2869 Fragments arranged by size: 2869 2007 786 738 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6697 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6697 BssSI C'ACGA_G Cuts at: 4757 5769 4757 Size: 1012 5685 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6697 BstBI TT'CG_AA Cuts at: 2752 5501 2752 Size: 2749 3948 BstEII G'GTnAC_C Cuts at: 1184 2684 1184 Size: 1500 5197 BstYI r'GATC_y Cuts at: 1972 2086 3980 4027 4394 4779 4796 6248 6334 Size: 114 1894 47 367 385 17 1452 86 Cuts at: 6334 6346 6692 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1894 1452 385 367 346 114 86 47 17 12 Bsu36I CC'TnA_GG Cuts at: 2637 2637 Size: 6697 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6145 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5958 Fragments arranged by size: 5958 683 56 BtsI GCAGTG_nn' Cuts at: 4213 5007 5027 4213 Size: 794 20 5883 Fragments arranged by size: 5883 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6697 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3853 3888 529 Size: 35 3289 35 3338 Fragments arranged by size: 3338 3289 35 35 DraI TTT'AAA Cuts at: 4001 4001 Size: 6697 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5704 809 Size: 507 4388 1802 Fragments arranged by size: 4388 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3406 4144 5033 Size: 54 1425 50 275 18 1576 738 889 Cuts at: 5033 6662 8 Size: 1629 43 Fragments arranged by size: 1629 1576 1425 889 738 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4144 1537 Size: 275 2332 4090 Fragments arranged by size: 4090 2332 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2240 3858 4627 1353 Size: 887 1618 769 3423 Fragments arranged by size: 3423 1618 887 769 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5284 5658 5804 387 Size: 1064 138 3695 374 146 1280 Fragments arranged by size: 3695 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2103 2221 2276 3969 4760 5357 6144 Size: 393 471 118 55 1693 791 597 787 Cuts at: 6144 6412 1239 Size: 268 1524 Fragments arranged by size: 1693 1524 791 787 597 471 393 268 118 55 EcoO109I rG'GnC_Cy Cuts at: 3191 3731 3191 Size: 540 6157 EcoRI G'AATT_C Cuts at: 2717 4006 4384 2717 Size: 1289 378 5030 Fragments arranged by size: 5030 1289 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3750 3750 Size: 6697 FspI TGC'GCA Cuts at: 5203 5203 Size: 6697 HaeII r_GCGC'y Cuts at: 1678 5844 1678 Size: 4166 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2443 2475 2686 2718 5338 Size: 32 135 32 871 32 211 32 2620 Cuts at: 5338 5370 5412 5444 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2620 871 211 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4041 4287 669 Size: 988 281 2103 246 3079 Fragments arranged by size: 3079 2103 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6697 HpaI GTT'AAC Cuts at: 4287 4287 Size: 6697 KpnI G_GTAC'C Cuts at: 4022 4022 Size: 6697 MfeI C'AATT_G Cuts at: 4296 4296 Size: 6697 MluI A'CGCG_T Cuts at: 6573 6573 Size: 6697 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3572 5811 5995 1305 Size: 2267 2239 184 2007 Fragments arranged by size: 2267 2239 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3408 10 Size: 54 1425 343 1576 3299 Fragments arranged by size: 3299 1576 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2922 3082 3315 3531 4655 5014 5173 Size: 648 1756 160 233 216 1124 359 159 Cuts at: 5173 518 Size: 2042 Fragments arranged by size: 2042 1756 1124 648 359 233 216 160 159 MspA1I CmG'CkG Cuts at: 1606 3440 4113 4790 5938 6183 1606 Size: 1834 673 677 1148 245 2120 Fragments arranged by size: 2120 1834 1148 677 673 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6145 NdeI CA'TA_TG Cuts at: 387 3135 387 Size: 2748 3949 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6697 NotI GC'GGCC_GC Cuts at: 4144 4144 Size: 6697 NspI r_CATG'y Cuts at: 1132 1229 3081 4055 5600 1132 Size: 97 1852 974 1545 2229 Fragments arranged by size: 2229 1852 1545 974 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6697 PciI A'CATG_T Cuts at: 5596 5596 Size: 6697 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6697 PfoI T'CCnGG_A Cuts at: 2073 2997 2073 Size: 924 5773 PmeI GTTT'AAAC Cuts at: 4001 4001 Size: 6697 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4766 4798 6279 6311 4766 Size: 32 1481 32 5152 Fragments arranged by size: 5152 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6697 PsiI TTA'TAA Cuts at: 4267 4267 Size: 6697 PstI C_TGCA'G Cuts at: 830 3403 4049 830 Size: 2573 646 3478 Fragments arranged by size: 3478 2573 646 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6697 PvuII CAG'CTG Cuts at: 3440 3440 Size: 6697 SalI G'TCGA_C Cuts at: 1936 4039 1936 Size: 2103 4594 SbfI CC_TGCA'GG Cuts at: 4049 4049 Size: 6697 ScaI AGT'ACT Cuts at: 2488 4945 6657 2488 Size: 2457 1712 2528 Fragments arranged by size: 2528 2457 1712 SfcI C'TryA_G Cuts at: 826 1045 2111 2574 3249 3399 4045 5180 5861 Size: 219 1066 463 675 150 646 1135 681 Cuts at: 5861 6052 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1066 681 675 646 463 219 191 150 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6697 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6697 SmaI CCC'GGG Cuts at: 1872 4024 1872 Size: 2152 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2174 4801 5702 5964 820 Size: 197 926 231 2627 901 262 1553 Fragments arranged by size: 2627 1553 926 901 262 231 197 SnaBI TAC'GTA Cuts at: 493 4488 493 Size: 3995 2702 SpeI A'CTAG_T Cuts at: 152 3386 152 Size: 3234 3463 SphI G_CATG'C Cuts at: 1132 3081 4055 1132 Size: 1949 974 3774 Fragments arranged by size: 3774 1949 974 SspI AAT'ATT Cuts at: 5 52 4621 5 Size: 47 4569 2081 Fragments arranged by size: 4569 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6697 StyI C'CwwG_G Cuts at: 513 1065 4136 513 Size: 552 3071 3074 Fragments arranged by size: 3074 3071 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4724 4741 4894 5079 5524 325 Size: 1255 3144 17 153 185 445 1498 Fragments arranged by size: 3144 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2486 2758 2790 Size: 274 80 33 51 1443 509 272 32 Cuts at: 2790 3974 4943 6655 96 Size: 1184 969 1712 138 Fragments arranged by size: 1712 1443 1184 969 509 274 272 138 80 51 33 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2274 2306 6657 6689 2274 Size: 32 4351 32 2282 Fragments arranged by size: 4351 2282 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6697 XbaI T'CTAG_A Cuts at: 2726 4033 2726 Size: 1307 5390 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6697 XmaI C'CCGG_G Cuts at: 1870 4022 1870 Size: 2152 4545 XmnI GAAnn'nnTTC Cuts at: 4826 4826 Size: 6697 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3817 4505 276 Size: 53 83 186 936 2283 688 2468 Fragments arranged by size: 2468 2283 936 688 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI