Restriction map for
pFN21AE1847
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AvaII AvrII BamHI BbvCI BcgI
BfrI BglI BglII BlnI BpmI
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT22I FseI
HgaI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MamI MfeI
MflI MluI MroI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PleI PmeI PpuMI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 655 GTAGAC GT^[AC][GT]AC
AccII 39 CGCG CG^CG
AspI 405 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 800 TGCGCA TGC^GCA
BalI 735 TGGCCA TGG^CCA
BanII 77 GAGCCC G[GA]GC[CT]^C
BbrPI 1006 CACGTG CAC^GTG
BbsI 717 GTCTTC GAAGAC(2/6)
BciVI 767 GTATCC GTATCC(6/5)
BclI 278 TGATCA T^GATCA
BcnI 221 CCCGG CC^[GC]GG
BfaI 547 CTAG C^TAG
BmyI 77 GAGCCC G[AGT]GC[ACT]^C
BpuAI 717 GTCTTC GAAGAC(2/6)
BsaAI 1006 CACGTG [CT]AC^GT[GA]
BseRI 964 CTCCTC GAGGAG(10/8)
Bsp1286I 77 GAGCCC G[AGT]GC[ACT]^C
BspMI 194 ACCTGC ACCTGC(4/8)
BssHII 38 GCGCGC G^CGCGC
BstAPI 920 GCAGAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 39 CGCG CG^CG
Cfr13I 27 GGCCC G^G[ACGT]CC
EcoRV 774 GATATC GAT^ATC
EcoT14I 81 CCAAGG C^C[AT][AT]GG
FbaI 278 TGATCA T^GATCA
FspI 800 TGCGCA TGC^GCA
HinfI 902 GAATC G^A[ACGT]TC
HphI 281 TCACC GGTGA(8/7)
MaeI 547 CTAG C^TAG
MaeII 1007 ACGT A^CGT
MluNI 735 TGGCCA TGG^CCA
MscI 735 TGGCCA TGG^CCA
MseI 860 TTAA T^TAA
MvnI 39 CGCG CG^CG
NciI 221 CCCGG CC^[GC]GG
PflFI 405 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1006 CACGTG CAC^GTG
PmlI 1006 CACGTG CAC^GTG
PshAI 1074 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 383 CTGCAG CTGCA^G
Sau96I 27 GGCCC G^G[ACGT]CC
ScaI 588 AGTACT AGT^ACT
SexAI 406 ACCAGGT A^CC[AT]GGT
SfaNI 206 GCATC GCATC(5/9)
SfcI 383 CTGCAG C^T[GA][CT]AG
StuI 387 AGGCCT AGG^CCT
StyI 81 CCAAGG C^C[AT][AT]GG
TfiI 902 GAATC G^A[AT]TC
Tru9I 860 TTAA T^TAA
Tsp509I 145 AATT ^AATT
Tth111I 405 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 217 GGCGCC G[GA]^CG[CT]C
310 GGCGCC
BbeI 217 GGCGCC GGCGC^C
310 GGCGCC
BlpI 336 GCTGAGC GC^T[ACGT]AGC
470 GCTCAGC
Bpu1102I 336 GCTGAGC GC^T[ACGT]AGC
470 GCTCAGC
BsaHI 217 GGCGCC G[GA]^CG[CT]C
310 GGCGCC
BsgI 419 GTGCAG GTGCAG(16/14)
947 GTGCAG
BsmAI 355 GAGAC GTCTC(1/5)
867 GTCTC
BsmFI 21 GGGAC GGGAC(10/14)
910 GGGAC
BsrDI 105 CATTGC GCAATG(2/0)
598 GCAATG
BssSI 259 CACGAG CACGAG(-5/-1)
58 CTCGTG
CelII 336 GCTGAGC GC^T[ACGT]AGC
470 GCTCAGC
DdeI 337 CTGAG C^T[ACGT]AG
471 CTCAG
DsaI 569 CCACGG C^C[GA][CT]GG
669 CCACGG
EaeI 735 TGGCCA [CT]^GGCC[GA]
975 TGGCCG
FauI 29 CCCGC CCCGC(4/6)
242 GCGGG
HapII 222 CCGG C^CGG
680 CCGG
Hin1I 217 GGCGCC G[GA]^CG[CT]C
310 GGCGCC
HpaII 222 CCGG C^CGG
680 CCGG
KasI 217 GGCGCC G^GCGCC
310 GGCGCC
MboII 886 GAAGA GAAGA(8/7)
718 TCTTC
MspI 222 CCGG C^CGG
680 CCGG
NarI 217 GGCGCC GG^CGCC
310 GGCGCC
NspI 524 GCATGT [GA]CATG^[CT]
700 ACATGC
SfoI 217 GGCGCC GGC^GCC
310 GGCGCC
Tsp45I 403 GTGAC ^GT[GC]AC
446 GTGAC
TspRI 417 CAGTG CA[GC]TG[ACGT][ACGT]^
490 CACTG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AfaI AluI BanI BbvI
BsaJI BsiYI BslI BsmI BsrI
BssKI BstF5I BstNI Cac8I CfoI
DpnII EcoRII Fnu4HI FokI HaeII
HaeIII HhaI HinP1I ItaI MaeIII
MboI MnlI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI ScrFI TseI